A study on the needs to improve Korea abattoir's critical control point of HACCP system

Kim, Seongjoon,Choi, Kwanghoon,Myung, Donghoon,Chung, Hangsung,Kim, Sukwon,Choe, Nonghoon The Korean Society of Veterinary Science 2020 大韓獸醫學會誌 Vol.60 No.3

These days abattoirs' hygiene of Korea is regulated by Hazzard Analysis Critical Control Point (HACCP). Although 20 years have been left since first HACCP was adjusted in Korea, 12% of abattoirs got inconsistence on evaluations. Food poisoning caused by bacteria feces like pathogenic Escherichia coli and Salmonella has not decreased. These bacteria on meat cross-contaminate at the abattoir. Therefore, field verification of abattoir's critical control point (CCP) and experiments to find alternative ways of the CCP were conducted. The aerobic bacteria were measured before and after high-pressure water based washing process set as CCP in most abattoirs. Four parts of cattle carcasses were selected to apply sponge-swab method. The effects were < 1 log reduction which is not significant. Lactic acid (LC), chlorine dioxide (ClO₂) and slightly acidic electrolyzed water (SAEW) were used to measure the effect of reducing bacteria on beef by the different time. LC has 1.24-2.02 log reduction for aerobic bacteria. ClO₂ has 1.44-1.96 log reduction for aerobic bacteria. SAEW has 1.1-1.91 log reduction for aerobic bacteria. There was significant difference according to concentrations (p < 0.05). This study presents legitimacy for hygiene improvement of CCP by field verification. In addition, chemical disinfectants that can be mechanically applied have better reduction effects of high-pressure washing.

Tracing surrogate bacteria inoculated on hide through the beef slaughtering process

Kim, Seongjoon,Kim, Sukwon,Kim, Sung Kwan,Choi, Kwanghoon,Kim, Jinman,Choe, Nonghoon The Korean Society of Veterinary Science 2022 大韓獸醫學會誌 Vol.62 No.1

Many countries have imposed regulations relating to concerns that hide contamination will affect the cleanliness of abattoirs. However, South Korea has not indicated any clear criteria. The purpose of this study is to use surrogate bacteria to measure the contamination in abattoirs caused by contaminated cattle hides. The swab contact method and plate count method are used. Surrogate bacteria are found in most internal environments after the final process. These surrogates remained on the carcass even after the final washing process. This paper is the first study in South Korea that use surrogate bacteria to analyze contamination levels in abattoirs.

돼지 운송 차량과 운전자에 대한 도축장에서의 세척 및 소독 과정에 대한 고찰

김환주 ( Hwan-ju Kim ),김승채 ( Seung-chai Kim ),정창기 ( Chang-gi Jeong ),김재엽 ( Jae-yeob Kim ),김재홍 ( Jae-hong Kim ),백예찬 ( Ye-chan Baek ),정용진 ( Yong-jin Jung ),김원일 ( Won-il Kim ) 한국동물위생학회(구 한국가축위생학회) 2021 韓國家畜衛生學會誌 Vol.44 No.1

Various infectious diseases have threatened swine industries in Korea. One of the major risk factors of disease spread is livestock transport vehicle. For this reason, all abattoirs are required by law to have vehicle washing and disinfection facilities. However, detailed guidelines for cleaning and disinfection procedures were not provided. So in this study, assessment by visual observation was conducted to evaluate how well the cleaning and disinfection procedures of livestock transport vehicles have been performed in four different abattoirs in Jeonbuk, Korea. The observation included length of car washing time, the number of washing bay, type of washperson, type of disinfectant application, length of disinfection time, and compliance of driver’s hygienic procedure. Every vehicle in all abattoirs conducted washing and disinfection procedure. However, there were procedural differences for each abattoirs and defects in the washing and disinfection procedures. In addition to that, the compliance of driver’s hygienic procedure was not sufficient. Hence, detailed manuals on washing and disinfection procedures for involved people are required and it is necessary to establish an inspection and evaluation system.

Risk factors for COVID-19 outbreaks in livestock slaughtering and processing facilities in Republic of Korea

Seongju Choi,Tae Jong Son,Yeon-Kyung Lee 질병관리본부 2023 Osong Public Health and Research Persptectives Vol.14 No.3

Objectives: The goal of this study was to help prevent and control the spread of coronavirus disease 2019 (COVID-19) by identifying transmission routes and risk factors in livestock slaughtering and processing facilities (SPFs) and establishing an optimal intervention strategy for outbreaks.Methods: This case series study was a demographic analysis of patients with confirmed COVID-19 associated with 5 SPFs in Korea between January and June 2021. Additionally, in a retrospective cohort study, the association between COVID-19 infection and risk factors was analyzed for SPFs at which outbreaks occurred.Results: The COVID-19 attack rates were 11.2%, 24.5%, and 6.8% at 3 poultry SPFs (PSPFs) and 15.5% and 25.2% at 2 mammal SPFs (MSPFs). Regarding spatial risk factors, the COVID-19 risk levels were 12.1-, 5.2-, and 5.0-fold higher in the refrigeration/ freezing, by-product processing, and carcass cutting areas, respectively, than in the office area. The risk of COVID-19 infection was 2.1 times higher among employees of subcontractors than among employees of contractors. The COVID-19 risk levels were 5.3- and 3.0-fold higher in foreign workers than in native Korean workers in the PSPFs and MSPFs, respectively.Conclusion: As the COVID-19 pandemic continues, a detailed policy for infectious disease prevention and control intervention is needed, without interrupting economic activities. Thus, we propose an ideal intervention plan to prevent COVID-19 through disinfection and preemptive testing and to block its transmission through effective contact management during outbreaks at SPFs.

Bovine Tuberculosis of Korean Native Cattle in an Abattoir

Seong-Jun Jang(장성준),Sun-Hee Do(도선희),Mi-Ran Ki(기미란),Il-Hwa Hong(홍일화),Jin-Kyu Park(박진규),Ae Ri Ji(지애리),Kyu-Shik Jeong(정규식) 한국생명과학회 2009 생명과학회지 Vol.19 No.12

소 결핵은 만성적으로 서서히 진행 되어 쇠약해 지기 때문에 일반적으로 사후 검시 후에 확인 된다. 소결핵의 감염이 대부분 폐에서 발생하기 때문에 심한 경우에는 기침, 비루 및 호흡 곤란을 유발 한다. 본 고에서는 도축장에서 나온 사체의 심장, 간, 신장, 폐, 복수, 임파선 및 내장에서 얻은 시료를 분석한 것이다. 사체의 부검 검시도중 다양한 모양과 크기의 결핵 병변이 관찰 되었다. 백색 크림 색깔의 결핵은 화농성으로 임파선, 폐 및 장간막을 포함하여 흉막강에 퍼져 있었다. 폐와 임파선의 그램 염색과 Ziehl-Neelsen’s acid-fast 염색 결과 강한 조직화학적 양성 반응을 나타내었다. Acid-fast에 염색된 유기체들이 현미경 검경에서 발견 되었다. 본 고에서는 의심원인체로 사료되는 Mycobacterium bovis의 조직학적 발견을 근거로 이에 의한 소결핵의 조직병리학적 소견을 보고하는 바이다. Bovine tuberculosis is generally detected postmortem because it is a chronic debilitating disease. Since tuberculosis is mainly found in the lungs, clinical signs including coughing, nasal discharge, and difficulty breathing can occur in severe instances. In the present study, specimens were collected from the heart, liver, kidney, lung, pleural cavities, lymph nodes and intestines of carcasses found in an abattoir. According to post-mortem examination and inspection of carcasses, tuberculosis lesions were varied in appearance and size. Tubercles of a white cream color were disseminated throughout the pleural cavity including the lymph nodes, lungs and mesentery containing pus. Gram and Ziehl-Neelsen’s acid-fast staining for the lung and lymph nodes revealed a highly positive histochemical reaction. The acid-fast organisms were observed histologically in the lesions under a microscope. This report demonstrated the histopathology of bovine tuberculosis based on the histological findings of Mycobacterium bovis, which is a suspected causative agent.

Use of Duckweed, Bentonite and Acid to Improve Water Quality of Effluent Discharge from Abattoirs

Goopy, J.P.,Murray, P.J.,Lisle, A.T.,Al Jassim, R.A.M. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.8

Intensive animal industries create large volumes of nutrient rich effluent, which, if untreated, has the potential for substantial environmental degradation. Aquatic plants in aerobic lagoon systems have the potential to achieve inexpensive and efficient remediation of effluent, and to recover valuable nutrients that would otherwise be lost. Members of the family Lemnaceae (duckweeds) are widely used in lagoon systems, but despite their widespread use in the cleansing of sewage, only limited research has been conducted into their growth in highly eutrophic media, and little has been done to systematically distinguish between different types of media. This study examined the growth characteristics of duckweed in abattoir effluent, and explored possible ways of ameliorating the inhibitory factors to growth on this medium. A series of pot trials was conducted to test the tolerance of duckweed to abattoir effluent partially remediated by a sojourn in anaerobic fermentation ponds, both in its unmodified form, and after the addition of acid to manipulate pH, and the addition of bentonite. Unmodified abattoir effluent was highly toxic to duckweed, although duckweed remained viable and grew sub optimally in media with total ammonia nitrogen (TAN) concentrations of up to 100 mg/l. Duckweed also grew vigorously in effluent diluted 1:4 v/v, containing 56 mg TAN/L and also modified by addition of acid to decrease pH to 7 and by adding bentonite (0.5%).

Use of Chemical and Biological Agents to Improve Water Quality of Effluent Discharge from Abattoirs

Goopy, J.P.,Murray, P.J.,Lisle, A.T.,Al Jassim, R.A.M. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.1

Intensive animal industries create large volumes of nutrient rich effluent which, if untreated, has the potential for substantial environmental degradationand to recover valuable nutrients that would otherwise be lost. Members of the family Lemnaceae are widely used in lagoon systems, to achieve inexpensive and efficient remediation of effluent. Only limited research has been conducted into their growth in highly eutrophic media and there has been little done to systematically distinguish between different types of media. This study examined the growth characteristics of duckweed in abattoir effluent and explored possible ways of ameliorating the inhibitory factors to growth on this medium. A series of pot trials was conducted to test the tolerance of duckweed to abattoir effluent partially remediated by a sojourn in anaerobic fermentation ponds, both in its unmodified form and after the addition of acid to manipulate pH, and the addition of bentonite. Unmodified abattoir effluent was highly toxic to duckweed, even at dilutions of 3:1. Duckweed remained viable and grew sub-optimally in simplified media with total ammonia nitrogen (TAN) concentrations of up to 100 mg/L. Duckweed grew vigorously in effluent diluted 1:4 v/v, containing 56 mg TAN/L when modified by addition of acid (to decrease pH to 7) and bentonite at 0.5%. The results of this study suggest that bentonite plays an important role in modifying the toxicity of abattoir effluent to duckweed.

The Effects of Pre-slaughter Stress and Season on the Activity of Plasma Creatine Kinase and Mutton Quality from Different Sheep Breeds Slaughtered at a Smallholder Abattoir

Chulayo, A.Y.,Muchenje, V. Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.12

The objective of the current study was to determine the effect of pre-slaughter stress, season and breed on the activity of plasma creatine kinase (CK) and the quality of mutton. One hundred and seventy-three (173) castrated sheep from Dormer (DM), South African Mutton Merino (SAMM), Dorper (DP) and Blackhead Persian (BP) sheep breeds were used in the study. The animals were grouped according to age-groups as follows: Group 1 (6 to 8 months), Group 2 (9 to 12 months) and Group 3 (13 to 16 months). Blood samples were collected during exsanguinations using disposable vacutainer tubes for CK analysis. Representative samples of the Muscularis longissimuss thoracis et. lumborum (LTL) were collected from 84 castrated sheep, of different breeds (28 per breed) 24 h after slaughter. The following physico-chemical characteristics of mutton were determined; meat pH ($pH_{24}$), color ($L^*$, $a^*$ and $b^*$), thawing and cooking losses and Warner Braztler Shear Force (WBSF). The activity of plasma CK was significantly higher (p<0.001) in summer ($1,026.3{\pm}105.06$) and lower in winter ($723.3{\pm}77.75$). There were higher values for $L^*$ ($33.7{\pm}0.94$), $b^*$ ($11.5{\pm}0.48$) and WBSF ($29.5{\pm}1.46$) in summer season than in winter season; $L^*$ ($29.4{\pm}0.64$), $b^*$ ($10.2{\pm}0.33$) and WBSF ($21.2{\pm}0.99$). The activity of plasma CK was influenced by the type of breed with Dormer having the highest (p>0.001) levels ($1,358.6{\pm}191.08$) of CK. South African Mutton Merino had higher values for $pH_{24}$ ($5.9{\pm}0.06$), $L^*$ ($34.2{\pm}0.97$), $b^*$ ($12.2{\pm}0.50$) and WBSF ($26.8{\pm}1.51$) and Blackhead Persian had higher values ($35.5{\pm}2.17$) for cooking loss (CL%) than the other breeds. Computed Principal Component Analyses (PCA) on the activity of plasma CK and physico-chemical characteristics of mutton revealed no correlations between these variables. However, positive correlations were observed between $pH_{24}$, $L^*$, $a^*$, $b^*$, CL% and WBSF. Relationships between pre-slaughter stress, CK activity and physico-chemical characteristics of mutton were also observed. It was therefore concluded that although mutton quality and creatine kinase were not related, pre-slaughter stress, season and breed affected the activity of creatine kinase and mutton quality.

도축한우의 신장에 대한 병리학적 연구

노영선 ( Young Sun Roh ),윤여백 ( Yea Baek Yoon ),김영진 ( Yong Jin Kim ),이성희 ( Seong Hee Rhee ),레브코로지스키 ( Lev Kolodzieyski ),임병무 ( Byung Moo Rim ),임채웅 ( Chae Woong Lim ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.2

To examine the prevalence and types of kidney lesions In Korean cattle, a survey was carried out at a abattoir in September 2000 and January 2001. The collected kidneys were examined grossly and histopathologically and investigated for the patterns of renal diseases with considering season and sex. Of 735 cattle (99 bulls and 246 cows in September and 140 bulls, 3 steers, and 247 cows in January) surveyed, 301 (41%) cattle had various renal lesions. The most common prominent finding was focal or multiple interstitial nephritis (36.2%). Other lesions included focal or multiple renal cyst (9.1%), infarction <0.1%), hemorrhage (0.3%), and renal atrophy (0.3%). Microscopically, main pathological lesions were acute or chronic interstitial nephritis with moderate infiltration of neutrophil or lymphocyte, respectively. Finally, the kidney lesions are more or less correlated with the meat quality, especially in female Korean cattle.

Beef Supply Chains in Australia: Implications for Korean Beef Industry

( Rodney J. Cox ),( Zhang Yue Zhou ),( Jung Sup Choi ) 한국농촌경제연구원 2003 Journal of Rural Development Vol.26 No.1

Australia has over time developed an entire of beef supply chains approach involving the production, processing and distribution sectors to provide consistent high quality beef to consumers both domestically and overseas. The Australian beef supply chains have proved to be effective, which in turn have contributed to the further development and success of the beef industry. The Australian experience in developing and managing its beef supply chains can be of great relevance to beef industries of other countries. This paper highlights the development and management of the Australian beef supply chains and draws implications for the future development of Korean beef industry.