Surface modification of paclitaxel-loaded liposomes using d-α-tocopheryl polyethylene glycol 1000 succinate: Enhanced cellular uptake and cytotoxicity in multidrug resistant breast cancer cells

Han, Su-Min,Baek, Jong-Suep,Kim, Min-Soo,Hwang, Sung-Joo,Cho, Cheong-Weon Elsevier 2018 Chemistry and physics of lipids Vol.213 No.-

<P><B>Abstract</B></P> <P>Liposomes can achieve a controlled release and an improved bioavailability of water- insoluble drug with minimized side effects. Paclitaxel is an efficient anticancer drug for the treatment of various cancers. However, paclitaxel has a solubility of 0.5 mg/L in water and a low bioavailability of 6.5%. Moreover, paclitaxel is a substrate for p-glycoprotein, which shows a decreased accumulation of drug within the cancer cell expressed by a p-glycoprotein. Therefore, the purpose of this study is to prepare a paclitaxel-loaded liposome and evaluate the effect of d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) as an inhibitor of p-glycoprotein on the paclitaxel-loaded liposome. The paclitaxel-loaded liposome and TPGS coated paclitaxel-loaded liposome had spherical vesicles, with mean particle size 184.9 ± 18.45 nm with PDI 0.324 ± 0.018 and 282.6 ± 20.41 nm with PDI 0.269 ± 0.013, respectively. Paclitaxel-loaded liposome and TPGS coated paclitaxel-loaded liposome showed a controlled and sustained release of PTX over 72 h. The cellular uptake of paclitaxel from TPGS coated paclitaxel-loaded liposome was a 3.56-fold increase for 2 h and 5.75-fold increase for 4 h compared to that from paclitaxel-loaded liposome in MCF-7/ADR cells, resulting in improved cytotoxicity against MCF-7/ADR cells. Western blot assay revealed the P-gp inhibitory effect of TPGS-coated PTX-liposome. In conclusion, TPGS coated liposome with a sustained releasing capability and the inhibitory effect of p-glycoprotein may be a promising carrier for future applications in cancer therapy.</P> <P><B>Highlights</B></P> <P> <UL> <LI> TPGS coated PTX-liposome between the sizes of 220 nm to 280 nm were successfully prepared and TEM micrographs of the TPGS coated PTX-liposome showed a spherical shape and successful TPGS coating on its surface. </LI> <LI> The TPGS coated PTX-liposome allowed achievement of a more sustained release of PTX. </LI> <LI> An enhanced cellular uptake of PTX from the TPGS coated PTX-liposome was observed compared to that of PTX-liposome in MCF-7/ADR cells. </LI> <LI> The TPGS coated PTX-liposome showed a significantly higher cytotoxicity than the free PTX solution and PTX-liposome in all equivalent PTX solutions against MCF-7/ADR cells. </LI> <LI> Such a TPGS coated liposome may hold great advantages in terms of manipulating release profiles, and enhancing the stability and improving cytotoxicity of anticancer drugs for future exploitation in cancer therapy. </LI> </UL> </P>

당귀 추출물이 liposome 의 안정성 및 유동성에 미치는 영향

배송자,김경옥,노승배 신라대학교 자연과학연구소 1997 自然科學論文集 Vol.4 No.-

인지질 liposome의 안정성과 유동성에 대한 당귀 추출물의 영향을 알아보기 위해 여러 종류의 인지질을 만들어 실험한 결과 PC계 인지질 중 DPPC를 사용하여 인지질 liposome을 만들고, 막안 정성에 대한 영향을 calcein을 봉입한 liposome에 fluorescence self-quenching방법으로 측정하였으며, 막유동성에 대한 영향은 fluorescence polarization방법으로 P값을 측정하였고 그 결과를 다음과 같이 요약한다. 1. Phosphatidylcholine(PC)계 인지질 중 acyl chain의 C수가 14개인 dimyristoyl phosphatidylcholine(DMPC)과 C수가 16개인 dipalmitoyl phosphatidylcholine (DPPC) 및 여러 인지질의 혼합물인 egg phospatidylcholine(egg PC)을 사용하여 liposome 형성의 안정화와 막안정화에 대한 비교 실험을 하였다. 이중 liposome의 안정성이 가장 좋은 것은 egg PC였고, DPPC 그리고 DMPC의 순으로 calcein의 leakage %가 저하하였다. 2. 막 안정성에 미치는 당귀 추출물의 영향 : DPPC liposome에 당귀의 ethyl alcohol과 hexane 추출물을 가한 결과, DPPC만으로 만든 liposome보다 calcein leakage%가 감소하였으며, 추출용매에 다른 막안정화에 미치는 영향은 거의 유사하였다. 이와 같은 경향은 또한 인지질 liposome에 다른 약물을 첨가했을 경우 calcein의 leakage%정도보다 훨씬 그 영향이 탁월함을 보여주었다. 즉 당귀 추출물의 소수성 부분은 인지질 liposome의 상전이온도 이하(Tm. 41∼42℃)인 25℃에서 지질 이중 층의 rigid한 부분의 공간 속으로 깊숙히 들어가 막안정성 증가에 의한 인지질막의 condensing effect에 현저한 영향을 미침을 알 수 있었다. 3. 막 유동성에 미치는 당귀 추출물의 영향 : DPPC로 만든 liposome에 DPH로 labeling한 후 온도 증가에 따른 DPH의 fluorescence polarization값(P)의 측정에서 DPPC만으로 만든 liposome의 경우 상전이온도 이전의 gel 상태에서 상전이온도(phase transition temperature, Tm)로 되면서 P값이 현저히 저하하였으며, 당귀의 ethyl alcohol과 hexane추출물을 농도 별로 첨가했을 때는 35℃에서 시작하여 상전이온도 이전까지 현저히 P값의 저하가 일어남으로서 인지질 막의 유동성 증가를 알 수 있었으며 상전이온도 근처에서는 P값의 저하 경사가 완만해져서 온도의 증가에 따른 P값의 변화는 미미하였으나 상전이온도 이후에서는 DPPC liposome의 P값보다 당귀 추출물 첨가의 경우가 상대적으로 그 정도가 훨씬 증가된 상태였다. 이 결과에서 상전이온도 이전에서의 당귀 추출물의 막유동성 증가 효과를 읽을 수 있었고, 상전이를 전후해서 증가된 유동성은 상전이온도 이후에서는 온도 증가에 따라 미미하였으며, 오히려 상전이온도 이후에서는 DPPC만의 liposome보다 상대적으로 추출물의 농도 증가에 따른 P값의 변화가 미미하여 막유동성의 감소를 볼 수 있었다. 이상의 결과에서 당귀의 ethyl alcohol과 hexane 추출물의 소수성 부분은 DPPC의 상전이온도 이전에서는 인지질 liposome의 이중층으로 깊숙히 들어가서 막안정성과 막유동성을 증가 시키므로써 옛부터 보혈강장 및 정혈제로서 민간약제로 쓰이던 당귀 추출물이 인지질 세포막에 대한 생리적 활성을 일으킬 수 있는 촉진제로서의 가능성을 시사해 주었다고 볼 수 있다. We investigated the effects of the Angelica Radix extract on the physicochemical properties of biological membranes such as membrane stability and fluidity employing the phospholipid liposomal membranes as a biomembrane-mimetic system. The leakage of encapsulated calcein from the liposome and the fluorescence polarization of DPH labelled in the liposomal membrane were employed as the parameters of the stability and fluidity of the liposomal membrance. The results of the experiments showed that the addition of the Angelica Radix extract to the phospholipid exerted some effects stabilizing the barrier function of the membrane and increasing the fluidity of the membrane in proportion to the concentration of additive. These results suggest that the activities of the Angelica Radix extract to enhance stability and fluidity of the liposomal membrane might have implication in their biological activities.

Lactococcus sp. CU216이 생산하는 박테리오신을 함유한 pH Sensitive Liposome의 응용

박성수,김명희,한경식,오세종 한국축산식품학회 2004 한국축산식품학회지 Vol.24 No.1

The objective of this study was to control Kimchi fermentation using pH sensitive bacteriocin entrapping liposome(bacteriocin-liposome). The liposomes were prepared by the reverse-phase evaporation method from a mixture of DPPC(dipalmitoyl phosphatidylcholine, DPPE(dipalmitoyl phosphatidylethanolamine), DOPC(dioleoyl phosphatidylcholine) and cholesterol in a molar ration of 4:2:1:4. The bacteriocin-liposome was disruptured at pH 4 of buffer and was stable at alkaline pHs(6 and 7). Irrespective of the addition of the bacteriocin-liposomes, the pH of every Kimchi sample decreased to 5 during 5 days storage at 5$^{\circ}C$. Kimchi samples treated with bacteriocin-liposomes maintained pH 4 or higher, while Kimchi samples not treated with bacteriocin-liposomes exhibited pH 3.58 or lower. In general, the pH of Kimchi samples stored at 20$^{\circ}C$ decreased faster, compared to that of Kimchi samples stored at 5$^{\circ}C$. The pH of Kimchi samples treated with the bacteriocin-liposomes was 3.9 during 90 days storage, while that of the samples not treated with the bacteriocin-liposomes was 3.68 and 3.32 during 30 days and 90 days storages, respectively. Lactic acid bacteria in Kimchi treated with the bacteriocin-liposome grew relatively slow at 5$^{\circ}C$. The viable cell number of lactic acid bacteria increased up to 4${\times}$10$\^$7/ cells/ml and then decreased to 8${\times}$10$\^$6/ cells/ml during 90 days storage at 5$^{\circ}C$. 발효식품의 후산발효를 조절하기 위하여 pH sensitive liposome의 이용 가능을 검토하였다. Lactococcm sp. CU216이 생산하는 박테리오신은 L.. acidophilus를 제외하고는 대부분의 유산균주들에 생육억제 효과가 있는 것으로 확인되었으며, 이를 Octyl-Sepharose column으로 분획하여 정제하였다. 정제된 박테리오신을 dipalmitoyl phosphocholine, dipalmitoyl phosphoethanolamine, dioleoyl phosphocholine 및 콜레스테롤를 각각 4:2:1:4(㏖ ratio)의 비율로 섞은 liposome을 제조하여 최종적으로 pH sensitive bacteriocin-liposome을 제조하였다. 이렇게 제조된 liposome은 pH 4부근에서 대부분 유리되는 것으로 확인되었으나 pH 6이상에서는 일어나지 않았다. 또한 pH sensitive bacteriocin-liposome 한국의 전통 발효식품인 김치에 적용하여 저장시 pH의 저하를 억제시키는 것으로 확인되었다. 본 실험 결과, pH sensitive bacteriocin-liposome은 발효식품의 후산발효 억제에 적용될 수 있으며 추후에 요구르트를 포함한 다른 발효식품에 대한 추가적인 실험이 필요한 것으로 생각되었다.

Human a-Synucleins을 위한 형광표지 리포좀 결합분석법의 개발

김시경,최웅 충북대학교 의과대학 충북대학교 의학연구소 2004 忠北醫大學術誌 Vol.14 No.2

Purpose : To understand the mechanism of interaction between a-Synuclein and phospholipid membrane, a quantitative method analyzing the binding of a-Synuclein to the phospholipid membrane was invented with fluorescence-labelled phospholipid liposome and this method was applied to the human a-Synuclein mutants. Materials and Methods : The phospholipid liposome was made of cholesterol, phosphatidylcholine and phosphatidylserine. Wild type and pathogenic mutnats (A30P, E46K. A53T) of human a-Synuclein was expressed and purified as GST-fusion proteins. Liposome binding assay was done with phospholipid liposome and GST-fusion human a-Synucleins. Results : Liposome binding assay was optimised for several parameters such as the concentration of protein and liposome, incubation time and temperature. At this optimised condition, the strength of binding to the phospholipid liposome was observed as such A30P < A53T .wild type < E46K. Conclusion : Liposome binding assay invented and optimized in this study is expected to be a useful tool for examining the relationship between the structure and the function of a-Synucleins. 연구목적: Human a-Synuclein과 phospholipid membrane과의 상호작용기전을 이해하고자, fluorescence-labelled phospholipld liposome을 이용하여 a-Synuclein 의 phospholipid membrane bmding을 정량분석하는 기술블 개발하고, 이를 human a-Synuc1ein 의 mulant 에 적용 하고자 히였다. 대상 및 방법: Cholesterol. phosphatidylcholine, phosphatidyserine으로 구성된 phospholipid liposome을 만들어 GST-융합 재조합 단백질로서 발현된 human a-Synuclein의 wild type 및 pathogenic mutant (A30P, E46K, A53T)와의 binding을 측정하였다. 결과: 형광표지를 이용한 liposome binding assay를 개발하고, 단백질 및 phospholipid 농도, 반응시간 및 온도 등 여러 가지 조건을 최적화 하였다. 이 조건에서 human a-Synuclein의 phospholipid liposome에 대한 binding 강도는 A30P < A53T ~ wild tupe M < E46의 순서였다. 결론: 본 연구에서 개발한 liposome binding assay는 앞으로 a-Synuclein의 구조와 기능을 연구하는데 유용한 연구수단으로 이용될 수 있을 것으로 예상된다.

Factors influencing the physicochemical characteristics of cationic polymer-coated liposomes prepared by high-pressure homogenization

Chung, S.K.,Shin, G.H.,Jung, M.K.,Hwang, I.C.,Park, H.J. Elsevier 2014 Colloids and surfaces. A, Physicochemical and engi Vol.454 No.-

Nano-liposomes were prepared by the modified reverse phase evaporation method using high-speed and high-pressure homogenizers. The optimal conditions for preparation of non-coated liposome, chitosan-coated liposome, and eudragit EPO-coated liposome were investigated. The liposomal membrane was composed of soybean lecithin. Chitosan and eudragit EPO coated the external surface of liposomes by electrostatic interaction. The results show that the physicochemical properties (e.g., mean size, polydispersity index, surface charge, and encapsulation efficiency) of non-coated liposomes were affected by homogenization pressure, number of homogenizing cycles, and ratio of core material to lecithin. Chitosan coating increased the mean size of liposome. In the case of eudragit EPO coating, the mean size of liposome increased up to 0.2%; a further increase in the eudragit EPO concentration led to some changes of the mean size of eudragit EPO-coated liposome. The highest stability for 30 day was achieved with 0.3% chitosan-coated liposome and 0.3% eudragit EPO-coated liposome, respectively. The release property was influenced by the type of coating material; the chitosan and eudragit EPO coating layers on the non-coated liposome surface delayed the release of the core material. Overall, a core material to lecithin ratio of 1:3 and three homogenizing cycles under 1000bar were selected as the optimal processing conditions. In addition, 0.3% eudragit EPO-coated liposome was selected as the optimal coating.

Physicochemical Characterization and Skin Permeation of Liposome Formulations Containing Clindamycin Phosphate

Srinivasan Shanmugam,송충길,Santhoshkumar Nagayya-Sriraman,Rengarajan Baskaran,용철순,최한곤,Dae-Duk Kim,우종수,유봉규 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.7

This study was undertaken to evaluate the physicochemical properties and skin permeation of liposome formulations containing clindamycin phosphate (CP), especially when charge was imparted to the liposome. Five different liposome formulations were prepared using Phospholipon 85G (PL) and cholesterol (CH) by conventional lipid film hydration technique. Molar ratio of CH to PL was varied in the range of 0.16-1.0. Charged liposomes were prepared in the same way with addition of 1,2-dioleoyl-3-trimethylammonium-propane chloride salt (DOTAP) and 1,2-dimyristoyl-sn-glycero-3-phosphate monosodium salt (DMPA) as charge carrier lipid for cationic or anionic charge of the liposome, respectively. Fresh full-thickness mice skin was taken and used for skin permeation study using Keshary-Chien diffusion cell with 1.77 ㎠ diffusion area at 37℃. All liposome formulations prepared showed homogeneous size distribution with mean particle size of about 1 μm or less. Among the five liposome formulations prepared, formulation with the molar ratio of 0.5 showed the best result in the physicochemical properties such as polydispersity index, entrapment efficiency, size evolution, and ability of the liposome to retain CP as of entrapped in the vesicles. Charge-impartation of the formulation with cationic charge carrier lipid resulted in additional benefit in terms of inhibition of size evolution, the ability of the liposome to retain CP in the vesicles, and skin permeation. Steady state flux of the drug through the mice skin in the cationic liposome vesicles was 0.75 ± 0.01 μg/㎠h while that in the control (dissolved into mixed alcohol solution) was 0.17 μg/㎠h. One half molar ratio of CH to PL was optimal in terms of physicochemical properties of the liposome formulation containing CP, and incorporation of cationic charge carrier lipid appeared to provide additional benefits for the stability of the liposome formulation and skin permeation of the drug.

Geranium maculatum 리포좀의 미백 및 항염 효과

최인정 한국피부과학연구원 2023 아시안뷰티화장품학술지 Vol.21 No.1

Purpose: In this study, cytotoxicity and whitening effects of liposomes and non-liposomes containing Geranium maculatum (G. maculatum) were measured to confirm their functionality as a cosmetic material. Methods: The cytotoxicity test was used to measure the effect of G. maculatum in liposomes and non-liposomes on B16F10 cells, and the whitening test was performed to measure the inhibitory effect of G. maculatum in liposomes and non-liposomes on melanin and tyrosinase production. Furthermore, the effect of G. maculatum liposomes on LPS-induced NO production was determined in RAW 264.7 cells. Cell viability was measured using the MTT assay. Results: G. maculatum in liposomes showed higher a cytotoxicity to B16F10 cells than G. maculatum in non-liposomes. Examination of the inhibitory effect on melanin production and tyrosinase production revealed that G. maculatum in liposomes produced a stronger whitening effect than that in non-liposomes. In addition, the experiments using RAW 264.7 cells showed that liposomes containing G. maculatum not only inhibited NO production but also resulted in higher cell viability than non-liposomes containing G. maculatum. Conclusion: Liposomes containing G. maculatum exert an antiseptic effect through a significant inhibition of NO production. They are preferred as a cosmetic material owing to their inhibitory activity on tyrosinase and melanin production. 목적: 본 연구에서는 Geranium maculatum 리포좀이 화장품 원료로서의 기능성을 확인하기 위해 Geranium maculatum을 함유한liposome과 non liposome을 이용하여 세포독성 및 미백실험을 실시하였다. 방법: 세포 독성 실험에서는 B16F10에 대한 세포 활성을 측정하였으며, 미백 측정으로는 melanin 생성 억제 효과 및 tyrosinase 생성 저해 효과를 측정하였다. 또한, 세포실험에서는 RAW 264.7 cell을 사용하여 LPS로 유도된 NO 생성량에 미치는 영향을 확인하였으며, 생존율 실험에는 MTT assay를 실시하였다. 결과: 실험결과 B16F10에 대한 세포독성 측정에서는 non liposome보다 liposome 세포독성이 낮음을 확인할 수 있었다. Melanin 생성 억제효과 및 tyrosinase 생성 억제 효과에서는 non liposome보다 liposome이 더 높은 미백효과를 나타내었다. 또한 RAW 264.7 cell을 이용한 실험에서는 Geranium maculatum을 함유한 li posome이 non liposome에 비해 NO 생성을 억제할 뿐 아니라 높은 생존율을 확인할 수 있었다. 결론: 따라서 Geranium maculatum을 함유한 liposome이 NO 생성량을 유의적으로 억제하여 함염효과가 있는 것으로판단되며, tyrosinase 저해 활성과 melanin 생성 억제 활성을 보여 미백 화장품 소재로 사용하는 것이 바람직할 것으로 사료된다.

난황 유래 인지질로 합성한 리포좀의 물리적 특성과 안정성

박선현,김명희 한국축산식품학회 2008 한국축산식품학회지 Vol.28 No.5

Liposomes were prepared from egg yolk phospholipids to study their physical properties and stability at various storage conditions. Under storage conditions at different pH levels, the particle sizes of liposomes increased at the range of pH 1-2, and the absolute values of ζ-potentials were reduced at the range of pH 1-4. The leakage of sulforhodamine B (SRB), a fluorescent dye which is encapsulated in the liposome, increased greatly at pH 2-4. At different storage temperatures, the particle size of liposomes increased from the 10 days of storage at 4oC and the 40 days at 20 and 35oC. The ζ-potentials decreased slightly later during storage under 4, 20 and 35oC. At the storage temperature of 50oC, the leakage of SRB was the greatest. Therefore, we concluded that the pH conditions lower than pH 6 and high temperature of 50oC are not conducive to storing liposomes. The results obtained here may prove helpful in developing liposome-based encapsulation and diagnostic reagents Liposomes were prepared from egg yolk phospholipids to study their physical properties and stability at various storage conditions. Under storage conditions at different pH levels, the particle sizes of liposomes increased at the range of pH 1-2, and the absolute values of ζ-potentials were reduced at the range of pH 1-4. The leakage of sulforhodamine B (SRB), a fluorescent dye which is encapsulated in the liposome, increased greatly at pH 2-4. At different storage temperatures, the particle size of liposomes increased from the 10 days of storage at 4oC and the 40 days at 20 and 35oC. The ζ-potentials decreased slightly later during storage under 4, 20 and 35oC. At the storage temperature of 50oC, the leakage of SRB was the greatest. Therefore, we concluded that the pH conditions lower than pH 6 and high temperature of 50oC are not conducive to storing liposomes. The results obtained here may prove helpful in developing liposome-based encapsulation and diagnostic reagents

탄성 리포좀을 사용한 쿼세틴의 경피 전달

박수남(Soo Nam Park),임명선(Myoung Sun Lim),박민아(Min A Park),권순식(Soon Sik Kwon),한샛별(Seat Byeol Han) 한국고분자학회 2012 폴리머 Vol.36 No.6

본 연구에서는 일반 리포좀의 단점을 보완하기 위하여 인지질(PC)과 계면활성제(Tego(R) care 450)로 구성된 탄성 리포좀을 제조하였다. 탄성 리포좀의 유효성분으로 천연 항산화제로 알려진 쿼세틴을 담지하였고, 탄성 리포좀 의 입자크기 및 가변형성과 쿼세틴의 포집 효율, 안정성, in vitro 피부투과를 평가하였다. 쿼세틴을 담지한 탄성 리 포좀의 평균 입자크기는 208.2~303.4 nm이였고, 포집효율은 64.1~87.5%로 측정되었다. 0.1% 쿼세틴을 담지한 탄성 리포좀 중에서 인지질과 계면활성제 비율이 90: 10인 경우가 가장 높은 포집효율(87.5%)과 가변형성 지수(28.3)를 나타내었다. 이 제형을 대상으로 피부 투과 실험을 진행하였다. 그 결과 대조군으로 사용된 일반 리포좀(114.8 μg/ cm2)과 1,3-butylene glycol(75.1 μg/cm2) 용액보다 탄성 리포좀의 피부 투과능(164.6 μg/cm2)이 훨씬 더 크게 나타났 다. 이러한 결과들로 미루어 보아 Tego(R) care 450을 이용한 탄성 리포좀이 피부를 통한 유효성분 전달에 유용하게 이용될 수 있음을 확인하였다. In this study, the elastic liposome consisted of egg phospholipids and edge activator (Tego(R) care 450) was prepared in order to supplement the defect of the conventional liposome. We prepared elastic liposome containing quercetin, known as natural antioxidant, and evaluated the vesicles size, elasticity, loading efficiency, stability, and in vitro skin permeation. The mean diameter of quercetin loaded elastic liposome formulations ranged between 208.2~303.4 nm and loading efficiency was observed 64.1~87.5%. The highest loading efficiency (87.5%) and deformability (28.3) were observed at the optimal ratio of 90: 10 (egg phospholipids: Tego(R) care 450) among 0.1% quercetin loaded elastic liposome formulations. The elastic liposome formulation was selected for further transdermal permeation study. The elastic liposome (129.9 μg/cm2) exhibited more skin permeability than general liposome (114.8 μg/cm2) and 1,3-butylene glycol (75.1 μg/ cm2) solution. This results suggest that the elastic liposome formulation using Tego(R) care 450 as a major edge activator could be useful for the delivery of active ingredient through the skin transdermal.

Enhanced transdermal delivery and optimization of nano-liposome preparation using hydrophilic drug

박준범,Hyung-Gon Noh,Hyung-gon Noh,Jung-Mi Kim,강진양 한국약제학회 2012 Journal of Pharmaceutical Investigation Vol.42 No.2

The aim of this study is the appropriate manufacture of nano-liposome and evaluation of transdermal drug delivery after drug was loaded in liposome for topical site especially nose. To make small, stable, uniform and highly encapsulated nano-liposome, many factors including solvent, stabilizer, pH balance, homogenization speed,sonication time and filtration are critical. Especially, stearate series surfactants have positive effects on liposome size and long-term stability. In this experiment, unsaturated lecithin and Pseudoephedrine HCl (PSE) were selected for the surfactant and model drug, respectively. The unsaturated lecithin, one of the surfactants used to make the liposome, has excellent skin affinity and penetration. Tween 20 and Tween 80, support emulsifying agents,propylene glycol, a lecithin solvent and triethanolamine as stabilizer were also used as materials for liposome. Nanoliposome was made with a high shear homogenizer and pulverized by ultrasonicator to reduce the size and increase uniformity. After that, a transdermal experiment was conducted with Franz-cells on hairless mouse skin and PSE was determined by HPLC. Based on the results of the experiments, when the appropriate concentrations of support emulsifying agent and stabilizer were added, the average size of the liposome was about 300 nm and the encapsulation rate was close up to 40%. Moreover, with faster mixing speed and longer sonication time, more uniform and smaller particles tended to be manufactured. In the skin permeation test, PSE entrapped in liposome had 20 times higher permeability than PSE raw material.