추출 방법과 조건에 따른 소나무 지엽 추출효율 변화

김동성(Dong Sung Kim),김형민(Hyung Min Kim),성용주(Yong Joo Sung),강석구(Seog Goo Kang),강호양(Ho-Yang Kang),이준우(Jun-Woo Lee),김세빈(Se Bin Kim),박관수(Gwan-Soo Park) 한국펄프·종이공학회 2016 펄프.종이技術 Vol.48 No.1

The extraction efficiency depending on the extracting methods and the conditions of extraction was investigated. The common steam extraction was compared to the distillation extraction method. The effects of the samples size and the extraction time on the extract yield were also investigated by using UV-Vis spectrophotometer. One of the functional components of pine needle extract as the natural phenol base components were detected by the UV-VIS at around 235 nm wavelength range. The absorbance intensity at around 235 nm wavelength of the pine needle extract was used as the indicator of the extraction efficiency in this experiment. The distillation extraction showed the higher extract yield than the steam extraction. The grinding treatment of pine needles also resulted in the better extract performance, but the severe grinding showed a little decrease in the extract yield especially in case of the distillation extraction method. More than half of the extract was collected at the first stage of the extraction, that was the first 15 minutes in the total 60 minutes extraction.

잣송이 부산물로부터 수증기 증류법에 의한 피톤치드의 추출

김배용 ( Bae Yong Kim ),이철태 ( Chul Tae Lee ) 한국공업화학회 2015 공업화학 Vol.26 No.6

Extraction of phytoncide oil from korea pine cone waste without damaging the pine cone tree itself was investigated using a steam distillation method. Also various components in the extracted phytoncide oil were separated using a column chromatography method. The extraction of phytoncide oil was effectively proceeded, and the maximum production yield of phytoncide oil could be obtained under 100 ℃ of distillation temperature and within 30 minute of distillation time. According to chemical analysis, it was found that the phytoncide oil from korea pine cone waste was consisted of more than 12 components such as α-pinene, β-pinene, D-limonene, as main components. In addition, the aqueous hydrogel containing other components such as verbenone, α-terpinieol, fenchol, different from components of phytoncide oil itself could be obtained through the steam distillation.

딸기의 휘발성 향기성분의 초임계 유체 추출

이해창(Hae-Chang Lee),서혜영(Hye-Young Seo),신동빈(Dong-Bin Shin),박용곤(Yong-Kon Park),김윤숙(Yoon-Sook Kim),지중룡(Joong-Ryong Ji),최희돈(Hee-Don Choi) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.6

SFE법을 이용하여 딸기의 휘발성 향기성분을 추출하기 위한 시료의 적정 전처리 및 SFE 작동조건을 검토하였으며, SDE법 및 SE법 등의 추출방법과 휘발성 향기성분 조성을 비교하였다. 딸기 생시료, 동결건조시료, 그리고 생시료에 celite를 30, 70%를 혼합하여 제조한 시료를 이용하여 SFE 처리후 향기성분을 분석한 결과, 생시료에 celite를 70% 첨가하는 방법이 향기성분을 추출하는데 가장 효과적인 것으로 나타났다. 온도(40, 55℃)와 압력(3,000, 6,000 psi)을 달리하여 SFE 처리한 결과, 3000 psi, 55℃ 조건에서 alcohol류와 acid류의 추출효율이 증가하였으며, γ-dodecalactone의 함량도 3배 정도 높은 수준으로 나타났다. SDE법, SFE법 및 SE법 등의 추출방법별 딸기의 휘발성 향기성분을 분석한 결과, SFE법은 SDE법에 비해 추출한 휘발성 향기성분의 종류는 적지만 추출량이 비슷하면서 열분해 및 열변성이 발생하지 않아 딸기 고유의 향기에 가까운 향기패턴을 나타내었으며, 기호적으로 우수한 acid류가 다량 함유된 향기성분을 추출하기에 적합한 것으로 나타났다. In order to optimize the supercritical fluid extraction (SFE) conditions of volatile components from the strawberry, we conducted an evaluation of the sample preparation and SFE operating conditions. The analysis of the volatile components extracted by a variety of sample preparation protocols led to the identification of 30, 26, 30, and 34 volatile components in fresh, freeze-dried, 30% celite and 70% celite treatments, respectively. The 70% celite treatment was the most effective in extracting the volatile components from strawberry via SFE. Analysis of the volatile components extracted by a variety of SFE operating conditions yielded identifications of 34, 35, 34, and 35 volatile components at 3,000 psi (40, 50℃) and 6,000 psi (40, 50℃), respectively. The extraction yield of alcohols and acids, and the total volatile component contents, were highest under conditions of 3,000 psi and 55℃. Volatile components from the strawberry were extracted via SFE, simultaneous steam distillation and extraction (SDE), and solvent extraction (SE). The analysis of the volatile components extracted via different extraction methods resulted in the identification of 56, 34, and 32 volatile components in the SDE, SFE, and SE extracts, respectively. The total volatile component contents identified in the SDE, SFE, and SE extracts were 20.268±1.144, 21.627±1.215 and 2.476±0.177 mg/kg, respectively. The SFE extract evidenced higher contents of sweet flavors such as 2-methylbutanoic acid, 2-methylpropanoic acid, and hexanoic acid than the SDE and SE extracts. SFE proved to be the most appropriate method for the extraction of fresh volatile components from the strawberry.

Antioxidant Activities of Aroma Extracts in Commercially Available Red Wines in Korea

Koan Sik Woo,Geonuk Jeon,Hyang-Sik Yoon,Heon-Sang Jeong,Junsoo Lee 한국식품영양과학회 2008 Preventive Nutrition and Food Science Vol.13 No.3

The antioxidant activities of aroma extracts from commercially available red wines in Korea were evaluated. The aroma extracts of the red wines were extracted by simultaneous steam distillation. Antioxidant activity was measured by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity and ABTS (2,2-azino-bis-3-ethylbenzothiazoline- 6-sulphonic acid) radical cation scavenging activity. DPPH radical scavenging activity of the aroma extracts in the red wines increased with increases in the amount of wine used for aroma extraction. Antioxidant activities of domestic wine 1, imported wine 7, and imported wine 12 were 97.16, 96.72 and 94.52%/20 mL wine by DPPH assay and 7.09, 8.07 and 7.28 mg ascorbic acid equivalents per mL wine by ABTS assay, respectively. This study demonstrates potent antioxidant activities of the aroma extracts of commercially available red wines in Korea.

Antioxidant Activities of Aroma Extracts in Commercially Available Red Wines in Korea

Woo, Koan-Sik,Jeon, Geon-Uk,Yoon, Hyang-Sik,Jeong, Heon-Sang,Lee, Jun-Soo The Korean Society of Food Science and Nutrition 2008 Preventive Nutrition and Food Science Vol.13 No.3

The antioxidant activities of aroma extracts from commercially available red wines in Korea were evaluated. The aroma extracts of the red wines were extracted by simultaneous steam distillation. Antioxidant activity was measured by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity and ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid) radical cation scavenging activity. DPPH radical scavenging activity of the aroma extracts in the red wines increased with increases in the amount of wine used for aroma extraction. Antioxidant activities of domestic wine 1, imported wine 7, and imported wine 12 were 97.16, 96.72 and 94.52%/20 mL wine by DPPH assay and 7.09, 8.07 and 7.28 mg ascorbic acid equivalents per mL wine by ABTS assay, respectively. This study demonstrates potent antioxidant activities of the aroma extracts of commercially available red wines in Korea.

목향과 향부자 추출물이 흰쥐의 만성 스트레스에 미치는 실험적 효과

최찬헌,홍준영,정현우 한의병리학회 2022 동의생리병리학회지 Vol.36 No.2

This study aimed to investigate the anti-stress function of Aucklandiae Radix (AR) and Cyperi Rhizoma (CR). The essential oils used in the experiment were extracted from AR and CR using Steam Distillation Extraction and Super critical CO2 extraction. To observe the effects of sample administration, we measured feed intake, leukocytes, red blood cells, hemoglobin, platelets, serum serotonin content, immobility time, climbing time, and swimming time in mice subjected to chronic restraint stress as behavioral changes. The average body weight of all experimental groups increased than the average body weight of the control group. The immobility and climbing times of experimental groups A and B administered with supercritical extraction samples were shorter than those of the other experimental groups and the control group, and the swimming time was longer. The serotonin content in the blood of all experimental groups decreased compared to the normal group, and the serotonin content of the control group was increased. The authors suggest that Korean herbal medicines AR and CR may be utilized as anti-stress flavoring agents based on the above results.

추출방법에 따른 섬백리향의 휘발성 향기성분 비교

이사은 ( Sa Eun Lee ),김성문 ( Songmun Kim ),임원철 ( Won Churl Lim ),강기춘 ( Ki Choon Kang ),표형배 ( Hyeong Bae Pyo ) 대한화장품학회 2014 대한화장품학회지 Vol.40 No.2

본 연구에서는 각 추출방법에 따른 섬백리향(Thymus magnus Nakai)의 향기 성분들을 분석하여, 이를 토대로 본연의 향에 아주 가까우면서 맡기에 좋은 향으로 재현하고자 초임계유체추출법, 혼합증류법, 동시증류추출법으로 정유를 추출한 후 gas chromatography-mass spectrometry (GC-MS)로 휘발성 향기성분을 분석하였다. 그 결과 먼저 초임계유체추출법의 40 ℃ - 400 bar 조건에서 추출한 정유 성분 중 linalool (0.1%), trans-sabinene hydrate (0.9%)가 다른 조건에서 추출한 정유성분과 비교하였을 때 상대적으로 더 많이 함유되어 있었으며, 50 ℃ - 400 bar 조건에서는 borneol (3.82%), terpinen-4-ol (0.3%), caryophyllene oxide (2.2%), 50 ℃ - 300 bar 조건에서는 β-bisabolene (5.88%), 1-octen-3 -ol (0.31%), caryophyllene (2.91%), p-cymene (2.04%), γ-terpinene (0.52%), 50 ℃ - 200 bar 조건에서는 thymol (77.63%), carvacrol (5.65%)이 상대적으로 더 많이 함유되어 있는 것으로 나타났다. 혼합증류법으로 추출한 정유 성분 중에는 α-bisabolol (0.17%), caryophyllene (6.46%), cis-α-bisabolene (1.52%), β-bisabolene (20.65%)이 초임계유체추출법으로 추출한 정유 성분 보다 상대적으로 더 많이 함유되어 있는 것으로 나타났으며, 동시증류추출법을 통해서는 정유를 얻지 못하였다. 이 같은 연구 결과는 각각의 추출법을 통해서 확인된 향기 성분들을 모두 취합하여 섬백리향 본연의 향에 좀 더 가깝도록 세밀하게 재현하는데 있어 유용한 자료로 활용될 수 있을 것으로 보인다. The purpose of this study was to analyse the volatile components of Thymus magnus Nakai extracted by different extraction methods and reproduce scent close to original plant based on the results. For this purpose, the essential oil of T. magnus was extracted by supercritical fluid extraction (SFE), water and steam distillation (WSD) and simultaneous steam distillation and extraction (SDE) methods. The compositions of the essential oil were analyzed by gas chromatography-mass spectrometry (GC-MS). Consequently, linalool (0.1%) and trans-sabinene hydrate (0.9%) contents in the essential oil extracted by SFE method of 40 ℃ - 400 bar condition were relatively higher than compositions of the essential oil extracted by different conditions. The contents of borneol (3.82%), terpinen-4-ol (0.3%) and caryophyllene oxide (2.2%) were relatively higher at 50 ℃ - 400 bar and the contents of β-bisabolene (5.88%), 1-octen-3-ol (0.31%), caryophyllene (2.91%), p-cymene (2.04%) and γ-terpinene (0.52%) were extracted relatively higher at 50 ℃ - 300 bar. The compositions of the essential oil extracted by SFE method of 50 ℃ - 200 bar condition contained relatively higher contents of thymol (77.63%) and carvacrol (5.65%). The contents of α-bisabolol (0.17%), caryophyllene (6.46%), cis-α-bisabolene (1.52%) and β-bisabolene (20.65%) in the essential oil extracted by WSD method were relatively higher than compositions of the essential oil extracted by SFE method, and by SDE method we couldn't obtained essential oil. The results of this study could be utilized to reproduce scent close to original scent of T. magnus.

수증기증류조건에 따른 꽃향유 추출물의 품질특성

윤광섭 ( Kwang Sup Youn ),홍주헌 ( Joo Heon Hong ),최용희 ( Yong Hee Choi ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2006 한국식품저장유통학회지 Vol.13 No.5

국내 자생식물로부터 얻어진 천연정유 추출물의 항산화 효과에 대한 연구

김소연 ( Kim,S. Y. ),김성문 ( S. M. Kim ),박세진 ( S. J. Park ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2019 강원 농업생명환경연구 Vol.31 No.1

Oxidative stress, which is induced by free radicals, causes various pathophysiological problems in the body. Natural essential oils derived from aromatic plants such as lavender and rosemary are used as additives in foods and cosmetics because of their aroma and antioxidant effects. The purpose of this study was to investigate the antioxidant activity of natural essential oils from Korean endemic plants. Natural essential oils including Mentha piperascens, Teucrium veronicoides, Citrus unshiu Peel, Angelica gigas Nakai, Zingiber officinale Roscoe, Artemisia annua L., Houttuynia cordata Thunb, Dendranthema zawadskii var. latilobum, Chrysanthemum morifolium, and Atractylodes macrocephala Koidz. were extracted by steam distillation extraction method, respectively. 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid) methods were performed to measure the free radical scavenging activity of each essential oil. We found that the essential oils from the Asteraceae family including Dendranthema zawadskii var. latilobum and Chrysanthemum morifolium showed more potent antioxidant activity than those from other plants. Our findings suggest that natural essential oils derived from Asteraceae family have free radical scavenging activity, which may be explored in the prevention of health problems.

Characteristics and Antioxidative Activity of Volatile Compounds in Heated Garlic (Allium sativum)

Koan Sik Woo,Hyang-Sik Yoon,Youn Ri Lee,Junsoo Lee,Dae Joong Kim,Jin Tae Hong,Heon Sang Jeong 한국식품과학회 2007 Food Science and Biotechnology Vol.16 No.5

The aroma characteristics and antioxidative activity of volatile compounds in heat-treated garlic (Allium sativum L.) were evaluated. The garlic was heated to various temperatures (100, 110, 120, and 130℃) for different lengths of time (1, 2, and 3 hr). The volatile compounds of heated garlic were extracted by simultaneous steam distillation extraction (SDE). Aroma compound profiles were analyzed by gas chromatography/mass spectrometry (GC/MS) and antioxidative activity was measured by 2,2-diphenyl-2-picrylhydrazyl (DPPH) assay and 2,2"-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) cation decolorization assay. The major aroma compounds were sulfur compounds such as dimethyl disulfide, 2-propen-1-ol, methyl-2-propenyl disulfide, dimethyl trisulfide, diallyl disulfide, methyl-2-propenyl trisulfide, and di-2-propenyl trisulfide. DPPH radical scavenging activity (EDA, %) and the ascorbic acid equivalent antioxidant activity (AEAC) of volatile compounds in heated garlic increased significantly with the increase of temperature and time (p<0.001). The EDA (%) and AEAC of raw garlic were 26.8%/10 mg garlic and 39.05 mg ascorbic acid equivalent per g sample. After heat treatment, the highest values were 40.50%/10 mg garlic for EDA (%) and 46.43 mg ascorbic acid equivalent per g sample for ABTS.