아토피 아동 가정내 집먼지 진드기 농도와 환경요인: 환자 대조군 연구

김성호,박동진,변혜정,이현수,오인보,심창선,김양호,윤충식,Kim, Sung-Ho,Park, Dong-Jin,Byun, Hyae-Jeong,Lee, Hyun-Soo,Oh, In-Bo,Sim, Chang-Sun,Kim, Yang-Ho,Yoon, Chung-Sik 한국환경보건학회 2012 한국환경보건학회지 Vol.38 No.3

Objectives: The purpose of this study was to determine house dust mite concentrations in living rooms and bedding materials in atopic case-control groups. Methods: Fifty four homes with children suffering atopic diseases as cases and fifty one homes without atopic diseases as controls were selected after diagnosis at three elementary schools in Ulsan City. Dust samples were collected from the living rooms and bedding materials in each home during summer and winter with a vacuum cleaner and analyzed for house dust mites using enzyme-linked immunosorbent assay (ELISA). Environmental factors and family history were surveyed during sampling. Results: Both species of house dust mite Dermatophagoides farina (Der f 1) and Dermatophagoides pteronyssinus (Der p 1) were found in all homes. Derf1 concentrations were much higher than Der P1 concentrations, both in atopic homes (1518.9 vs. 27.0 ng/g. dust, respectively) and non-atopic homes (810.8 vs. 44.3 ng/g. dust, respectively). Der f 1 concentrations were significantly higher in the atopic group than in the non-atopic group (atopic: 1518.9 ng/g.dust, non-atopic: 810.8 ng/g.dust, p = 0.035). However, total house dust mite Der p 1 concentrations were significantly higher in the non-atopic group than in the atopic group (atopic: 27.0 ng/g.dust, non-atopic: 44.3 ng/g.dust, p = 0.035). Multiple regression implied that mothers with family history of atopic diseases (OR = 4.79, 95% CI = 1.81-12.69), Der f 1 concentrations (OR = 1.74, 95% CI = 1.07-2.81), and air freshener use (OR = 4.60, 95% CI = 1.72-12.34) had significant associations with atopic children. Conclusion: House dust mite Der f 1 concentrations were associated with atopic children. This study suggests that parents should reduce house dust mite Der f 1 concentrations through environmental controls.

아토피 안심마을로 이주한 아토피 환아의 관찰연구

성현경,서경숙,손창규,Sung, Hyun Kyung,Seo, Kyoung Suk,Son, Chang Gue 대한한방소아과학회 2014 대한한방소아과학회지 Vol.28 No.1

Objectives This study aimed to evaluate the effects of atopic care program through observing atopic patients who have lived in atopic-free village located Geumsan of Chungnam province. Methods Seventeen children (boy 7, and girl 10) with atopic dermatitis had moved into atopic-free village of Geumsan, and they have been cared with atopic care program. Atopic dermatitis symptoms were measured every month using SCORAD (Scoring of Atopic Dermatitis) and EASI (Eczema Area and Severity Index) from March, 2012 to September, 2013. The changes of scores were analyzed using paired t-test. Results The median month of residence period was 19.9 months (range from 6 to 42 months). The atopic symptoms were significantly improved on SCORAD (p<0.01) and EASI (p<0.1) respectively. Conclusion Despite the limitation of the small number of study subjects, this study may provide the possibility of natural environment-based therapy for children with atopic dermatitis.

Oral administration of Lactococcus chungangensis inhibits 2,4-dinitrochlorobenzene-induced atopic-like dermatitis in NC/Nga mice

Choi, W.J.,Konkit, M.,Kim, Y.,Kim, M.K.,Kim, W. American Dairy Science Association 2016 Journal of dairy science Vol.99 No.9

<P>Interest is increasing in the potentially beneficial role of probiotics in the prevention and treatment of atopic diseases. In this study, we investigated the protective effects of Lactococcus chungangensis CAU 28(T) against atopic dermatitis using murine macrophage RAW 264.7 cells, human keratinocyte HaCaT cells, human mast cell line HMC-1 cells, and a 2,4-dinitrochlorobenzene-induced atopic dermatitis model (Nc/Nga mice). The results showed that L. chungangensis CAU 28(T) exhibited potent antiinflammatory activity by inhibiting the production of the proinflammatory mediators nitric oxide and prostaglandin E-2 in lipopolysaccharide-stimulated RAW 264.7 cells. Treatment with L. chungangensis CAU 28(T) reduced the release of beta-hexosaminidase and histamine in HMC-1 cells stimulated with mast cell activator compound 48/80. In addition, the back skin and ears of NC/Nga mice exhibited reduced histological manifestations of atopic skin lesions such as erosion, hyperplasia of the epidermis and dermis, and inflammatory cell infiltration. Oral administration of L. chungangensis CAU 28(T) suppressed the production of IL-4, IL-5, IL-12, IFN-gamma, tumor necrosis factor-alpha, and thymus- and activation-regulated chemokine (TARC) in skin lesions, indicating that it strongly drives the local immune system with efficacy comparable to that of tacrolimus, a topical immunomodulatory drug used for the treatment of atopic dermatitis. The findings indicate that L. chungangensis CAU 28(T) could be a novel probiotic candidate for controlling the symptoms of atopic dermatitis.</P>

아토피성 피부염 동물모델에서 Black currant seed oil의 비장세포 cytokine 및 조직병리학적 변화에 미치는 영향

이예서 ( Ye Seo Lee ),박교현 ( Kyo Hyun Park ),김배환 ( Bae Hwan Kim ) 한국미용학회 2016 한국미용학회지 Vol.22 No.3

This study investigated the cytokine excretion of spleen cells and histopathological changes in atopic-like dermatitis model, and evaluated the anti-atopic effects of black currant seed oil. BALB/c mice (five-week old) were divided into four groups: N (normal group, nontreated), C (control group, induced atopic dermatitis), PC (positive control group, Tacrolimus ointment treatment for induced atopic dermatitis), and E (experiment group, Black currant seed oil treatment for induced atopic dermatitis, E). During the experiment, no significant changes were observed in feed intake, water, intake, body weight. The INF-γ levels of spleen cells in PC and E showed a significant increase compared to those of C, and. IL-4 levels in PC and E decreased significantly than those of C (p<0.05). In histological observation using H&E staining, the tissue thickness of the epidermis and the degree of inflammatory cell infiltration were improved in PC and E compared to C. The numbers of mast cells by toluidine blue staining were significantly decreased in PC and E than C (p<0.05). The results of this study indicated that black currant seed oil can improve the atopic dermatitis by regulating the Th1/Th2 cytokine ratio and histopathological mechanism.

연구노트 : 신선초 추출물의 아토피 억제활성

김민아 ( Min A Kim ),손형우 ( Hyeong U Son ),남동윤 ( Dong Yoon Nam ),차용수 ( Yong Su Cha ),신용규 ( Yong Kyu Shin ),최용희 ( Yong Hee Choi ),이상한 ( Sang Han Lee ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2012 한국식품저장유통학회지 Vol.19 No.5

The purpose of this study was to evaluate the anti-atopic-dermatitis effect of Angelica keiskei extract using a DNFB-induced animal model of atopic-dermatitis symptoms. A. keiskei was prepared via extraction with DW, 50% ethanol, and 100% ethanol in addition to fresh juice. All the extracts reduced the thickness levels of the ears and ear epidermis against swelling by DNFB inducement, especially in the aqueous-extract-treated group. The mRNA expressions of matrix metalloproteinases (MMPs), however, were not observed, which may indicate that Angelica keiskei extract alleviates the atopic-dermatitis symptoms in an MMP-independent manner. Finally, the level of the inflammatory cytokine IL-4 was inhibited only in the juice-treated group, although the IL-13 level was inhibited in the juice-, 50%-ethanol-extract-, and 100%-ethanol-extract-treated groups in a dose-dependent manner, and was not inhibited in the aqueous-extract-treated group. Taken together, these results suggest that Angelica keiskei extract has an inhibitory effect on atopic dermatitis, and may be a useful biomaterial for the development of cosmeceuticals.

아토피 피부염 환자에서 라텍스에 대한 피부단자검사와 RAST 양성율 조사

우혜진(Hye Jin Woo),박현정(Hyun Jeong Park),김형옥(Hyung Ok Kim) 대한피부과학회 2000 대한피부과학회지 Vol.38 No.11

Background:Natural rubber latex has become an important health concern in recent years, particularly in risk groups. Atopic dermatitis is well known risk factor for latex allergy, but there is no data or study of latex allergy in Korea. Objective:The purpose of this study was to investigate the prevalence of latex sensitization in atopic dermatitis patients. Methods:A total of 154 patients presenting with various diseases for allergologic evaluation was studied. A questionnaire-based history was taken, skin prick tests(SPT) with latex glove extract were done. In SPT positive patients, serum total IgE and latex-specific IgE by radioallergosorbent test(RAST) were measured, and a use test was also performed. Results:A total of 77 subjects(50%) was atopic. There were positive SPT reactions to latex in 13/77(16.9%). There were only 7 positive to the latex RAST in SPT positive patients. Except one patient, there were no clinically relevant latex allergy by history and challenge tests. Conclusion:The results of this study suggest that the prevalence of latex sensitization in atopic dermatitis patients is quite high(16.9%), and in latex allergy diagnosis, SPT is more sensitive than RAST. (Korean J Dermatol 2000;38(11):1475~1480)

Gastrodia elata Blume Attenuates 2, 4-Dinitrochlorobenzene-induced Atopic Dermatitis-like Skin Lesions in Balb/c Mice and SD Rats

김나형,이명로,이영미 한국생약학회 2015 Natural Product Sciences Vol.21 No.2

Gastrodia elata Blume is a well-known kind of natural products used as a folk medicine for thousands of years. However, anti-atopic dermatitis-like effects of G. elata Blume had not been evaluated until now. The aim of the present study is to investigate the protective effects of water extract from the roots of G. elata Blume (GE) on 2, 4-dinitrochlorobenzene-induced atopic dermatitis-like skin lesions using balb/c mice. Combination treatment of GE (at a dose of 12.5 mg/kg body weight by administrated per os + 0.5 mg/cm2 as ointment to apply on ear and dorsal skin) was significantly inhibited spleen weight, ear thickness, levels of serum immunoglobulin E and number of mast cells, compared with that of 2, 4-dinitrochlorobenzene-included groups without GE. Furthermore, combination application by oral administration plus by ointment of GE significantly inhibited the histamine release from rat peritoneal mast cells. These results suggest that combination treatment of oral administration plus ointment form of GE could be helpful as a potentially natural pharmaceutical treatment on atopic-like dermatitis.

The Genome-Wide Expression Profile of Saussurea lappa Extract on House Dust Mite- Induced Atopic Dermatitis in Nc/Nga Mice

임혜선,하혜경,신현규,정수진 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.9

Saussurea lappa has been reported to possess anti-atopic properties. In this study, we have confirmed the S. lappa’s anti-atopic properties in Nc/Nga mice and investigated the candidate gene related with its properties using microarray. We determined the target gene using real time PCR in in vitro experiment. S. lappa showed the significant reduction in atoptic dermatitis (AD) score and immunoglobulin E compared with the AD induced Nc/Nga mice. In the results of microarray using back skin obtained from animals, we found that S. lappa’s properties are closely associated with cytokine-cytokine receptor interaction and the JAKSTAT signaling pathway. Consistent with the microarray data, real-time RT-PCR confirmed these modulation at the mRNA level in skin tissues from S. lappa-treated mice. Among these genes, PI3Kca and IL20Rβ were significantly downregulated by S. lappa treatment in Nc/Nga mouse model. In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro. Therefore, our study suggests that PI3Kca-related signaling is closely related with the protective effects of S. lappa against the development of atopic-dermatitis.

Histopathological Comparison of Atopic-Like Dermatitis by Two Allergens in Nc/Nga Mice

Sehwan Shim,Sun Lim,Sung-Shik Shin,Jungkee Kwon 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.2

Atopic dermatitis (AD) consists of two main stages, the acute stage and the chronic stage. TH2 lymphocytes have a major contribution to AD immune response in acute stage, TH1 immune response in chronic stage. We here demonstrated the atopic-like dermatitis by repeated two allergens treatment, dermatophagoides pteronyssinus crude extract (DPE) and picryl chloride (PC), with histopathological investigation. Histopathologically, PC-treated Nc/Nga mice were demonstrated much more infiltration of macrophages and fibroblast, which are effecter cells in TH1 immune response. According to the differences of TH1/TH2 immune response, we suggest that AD model by DPE-treated, which are showed TH2 immune response, is more suitable for the observations of progressive histopathological change in AD skin lesions.

The Genome-Wide Expression Profile of Saussurea lappa Extract on House Dust Mite-Induced Atopic Dermatitis in Nc/Nga Mice

Lim, Hye-Sun,Ha, Hyekyung,Shin, Hyeun-Kyoo,Jeong, Soo-Jin Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.9

Saussurea lappa has been reported to possess anti-atopic properties. In this study, we have confirmed the S. lappa's anti-atopic properties in Nc/Nga mice and investigated the candidate gene related with its properties using microarray. We determined the target gene using real time PCR in in vitro experiment. S. lappa showed the significant reduction in atoptic dermatitis (AD) score and immunoglobulin E compared with the AD induced Nc/Nga mice. In the results of microarray using back skin obtained from animals, we found that S. lappa's properties are closely associated with cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway. Consistent with the microarray data, real-time RT-PCR confirmed these modulation at the mRNA level in skin tissues from S. lappa-treated mice. Among these genes, PI3Kca and $IL20R{\beta}$ were significantly downregulated by S. lappa treatment in Nc/Nga mouse model. In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not $IL20R{\beta}$ in vitro. Therefore, our study suggests that PI3Kca-related signaling is closely related with the protective effects of S. lappa against the development of atopic-dermatitis.