Emodin-induced Apoptosis in Human Breast Cancer BCap-37 Cells through the Mitochondrial Signaling Pathway

Zhiwei Huang,Guichen Chen,Ping Shi 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6

Emodin, a natural anthraquinone compound isolated from the rhizome of rhubarb, is reported to suppress the growth of tumor in many clinical situations. In this study, we focused on the effect of emodin in human breast cancer BCap-37 cells and further understand the underlying molecular mechanism in treating breast cancer. Using MTT assay and flow cytometry, we demonstrated the critical role of emodin in the suppression of the proliferation of BCap-37 cells based on a concentration- and time-dependent manner. The increase of apoptotic rate was also observed after incubation of BCap-37 cells on emodin at 20 μM and 50 μM for 48 h. The cells exhibited typical apoptotic features including cellular morphological change, chromatin condensation and membrane blebbing. The results of the study further showed that Bcl-2 level decreased, while Bax and cytosolic cytochrome c levels in sample cells increased after the emodin treatment by using Western blot. The decline in the Bcl-2/Bax ratio and the increase of cytosolic cytochrome c concentration were consistent with the increase of the apoptotic ratio. The results strongly suggest that the disruption of the mitochondrial signaling pathway was involved in emodin-induced apoptosis in BCap-37 cells.

Emodin-induced Apoptosis in Human Breast Cancer BCap-37 Cells through the Mitochondrial Signaling Pathway

Huang, Zhiwei,Chen, Guichen,Shi, Ping 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6

Emodin, a natural anthraquinone compound isolated from the rhizome of rhubarb, is reported to suppress the growth of tumor in many clinical situations. In this study, we focused on the effect of emodin in human breast cancer BCap-37 cells and further understand the underlying molecular mechanism in treating breast cancer. Using MTT assay and flow cytometry, we demonstrated the critical role of emodin in the suppression of the proliferation of BCap-37 cells based on a concentration- and time-dependent manner. The increase of apoptotic rate was also observed after incubation of BCap-37 cells on emodin at $20\;{\mu}M$ and $50\;{\mu}M$ for 48h. The cells exhibited typical apoptotic features including cellular morphological change, chromatin condensation and membrane blebbing. The results of the study further showed that Bcl-2 level decreased, while Bax and cytosolic cytochrome c levels in sample cells increased after the emodin treatment by using Western blot. The decline in the Bcl-2/Bax ratio and the increase of cytosolic cytochrome c concentration were consistent with the increase of the apoptotic ratio. The results strongly suggest that the disruption of the mitochondrial signaling pathway was involved in emodin-induced apoptosis in BCap-37 cells.

Excessive Daytime Sleepiness and Insomnia Symptoms in Adolescents With Major Depressive Disorder: Prevalence, Clinical Correlates, and the Relationship With Psychiatric Medications Use

Yudong Shi,Wei Li,Changhao Chen,Xiaoping Yuan,Yingying Yang,Song Wang,Zhiwei Liu,Feng Geng,Jiawei Wang,Xiangfen Luo,Xiangwang Wen,Lei Xia,Huanzhong Liu 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.11

Objective Excessive daytime sleepiness (EDS) and insomnia symptoms are common in patients with major depressive disorder (MDD), which might lead to a poor prognosis and an increased risk of depression relapse. The current study aimed to investigate the prevalence, and sociodemographic and clinical correlates of EDS and insomnia symptoms among adolescents with MDD.Methods The sample of this cross-sectional study included 297 adolescents (mean age=15.26 years; range=12–18 years; 218 females) with MDD recruited from three general and four psychiatric hospitals in five cities (Hefei, Bengbu, Fuyang, Suzhou, and Ma’anshan) in Anhui Province, China between January and August, 2021. EDS and insomnia symptoms, and clinical severity of depressive symptoms were assessed using Epworth sleepiness scale, Insomnia Severity Index, and Clinical Global Impression-Severity.Results The prevalence of EDS and insomnia symptoms in adolescents with MDD was 39.7% and 38.0%, respectively. Binary logistic regression analyses showed that EDS symptoms were significantly associated with higher body mass index (odds ratio [OR]=1.097, 95% confidence interval [CI]=1.027–1.172), more severe depressive symptoms (OR=1.313, 95% CI=1.028–1.679), and selective serotonin reuptake inhibitors use (OR=2.078, 95% CI=1.199–3.601). And insomnia symptoms were positively associated with female sex (OR=1.955, 95% CI=1.052–3.633), suicide attempts (OR=1.765, 95% CI=1.037–3.005), more severe depressive symptoms (OR=2.031, 95% CI=1.523–2.709), and negatively associated with antipsychotics use (OR=0.433, 95% CI=0.196–0.952).Conclusion EDS and insomnia symptoms are common among adolescents with MDD. Considering their negative effects on the clinical prognosis, regular screening and clinical managements should be developed for this patient population.

Elucidating synthesis of noble metal nanoparticles/graphene oxide in freescavenger γ-irradiation

Jie Shi,Wanwei Jiang,Liangsen Liu,Miaolei Jing,Fengyan Li,Zhiwei Xu,Xing-xiang Zhang 한국물리학회 2019 Current Applied Physics Vol.19 No.7

Three representative noble metal (Ag, Au and Pt) nanoparticles decorated graphene oxide (NMNPs/GO) composites were fabricated via γ-irradiation without scavenger. The NMNPs/GO composites exhibited the pure and well-dispersed particles structure, which directly illustrated that the GO could be acted as scavenger to benefit reduction and growth of NMNPs. Compared with irradiated GO (IGO), the GO substrate of composites had the lower relative content of hydroxyl groups, meanwhile, the relative contents of carbonyl groups (Pt > Au > Ag) were increased with increasing valence of noble metal. Such results illustrated that the abundant hydroxyl groups could convert the hydrated electrons, hydroxyl and hydrogen radicals to promote the growth of NMNPs on the surface of GO. In addition, the Raman signals of Ag NPs/GO composites were significantly enhanced (6.18 fold more than pristine GO, respectively), exhibiting obvious surface-enhanced Raman scattering activity. Therefore, this paper revealed that the GO could convert the hydrated electrons and radicals to synthetizing NMNPs/GO composites during γ-ray irradiation.

Dual-anchor anti-corrosion coating of copper foil for high-speed interconnects

Huijuan Shi,Guoyun Zhou,Qin Zhang,Pengju Wang,Yan Hong,Wei He,Shouxu Wang,Chong Wang,Zhiwei Han 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.126 No.-

In this experiment, two similar molecules with bifunctional groups have been characterized to prove theadvantages of dual-anchor molecular coating in the interfacial modification of the copper foil. The physicochemicalproperties of the modified copper foils have been studied. The obtained results indicate thatthe anti-corrosion performance and peeling strength were significantly improved for the copper foil treatedwith (2-(Methylthio)pyrimidin-4-yl)methanamine (MET), whose bonding information was analyzedvia the density functional theory calculation. It was found that the S-Cu and N-Cu bonds were bothformed on the Cu (1 1 1) surface for MET coating, and the adsorption of the double anchor pointsimproves the stability of the interface. The peeling test exhibits the function of MET coating as the adhesionpromotor between low-surface profile copper and resin for high-speed signal transmission.

Characteristics and Optimization of New Combined Magnetic Pole Negative-Salient Permanent Magnet Motor

Wang Wenqiang,Shi Liwei,Qiao Zhiwei,Zhu Yingjie,Ding Hongshan 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.3

With a wide speed range, high overload capacity and preventing permanent magnet demagnetization, negative-salient permanent magnet motors have a wide range of applications in the feld of electric vehicles. In view of the multiple operating conditions of the electric vehicle drive motor, this paper proposes a new type of combined magnetic pole negative-salient permanent magnet motor considering the driving conditions. Firstly, the optimization parameters of the motors are determined according to the analysis of the mathematical model. The design parameters are layered by the comprehensive sensitivity analysis, and the response surface Box-Behnken Design method and multi-objective genetic algorithm-II are used for optimization. Then, the electromagnetic performance of the proposed motor and the traditional interior permanent magnet motors are compared and analyzed through the fnite element simulation. Finally, a prototype is manufactured and the rationality of the proposed motor structure is verifed by experiments.

Robust Finite-horizon l2-l∞ Filtering for Discrete-time Nonhomogeneous Markovian Jump Systems Under Denial-of-service Attacks

Quangui He,Zhiwei Shi,Dingli Wu,Shisheng Fan,Zhengkuan Zhang,Deshuang Liu 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.7

The problem of robust finite-horizon l2-l∞ filtering is investigated for a class of discrete-time Markovian jump systems under denial-of-service attacks. The considered dynamic model is more appropriate, on account of polyhedral feature of time-varying transition probabilities. Denial-of-service attack that may jam or compromise the measurement packets on communication channels is assumed as a Bernoulli distributed sequence. Based on stochastic Lyapunov function approach, sufficient criteria corresponding to the error system are established for designing a finite-horizon l2-l∞ filter in the light of linear matrix inequalities. In the end, three illustrative examples are worked out to substantiate the theoretical results.

Acetylation of Smc3 by Eco1 Is Required for S Phase Sister Chromatid Cohesion in Both Human and Yeast

Zhang, Jinglan,Shi, Xiaomin,Li, Yehua,Kim, Beom-Jun,Jia, Junling,Huang, Zhiwei,Yang, Tao,Fu, Xiaoyong,Jung, Sung Yun,Wang, Yi,Zhang, Pumin,Kim, Seong-Tae,Pan, Xuewen,Qin, Jun Elsevier 2008 Molecular cell Vol.31 No.1

<P><B>Summary</B></P><P>Sister chromatid cohesion is normally established in S phase in a process that depends on the cohesion establishment factor Eco1, a conserved acetyltransferase. However, due to the lack of known in vivo substrates, how Eco1 regulates cohesion is not understood. Here we report that yeast Eco1 and its human ortholog, ESCO1, both acetylate Smc3, a component of the cohesin complex that physically holds the sister chromatid together, at two conserved lysine residues. Mutating these lysine residues to a nonacetylatable form leads to increased loss of sister chromatid cohesion and genome instability in both yeast and human. In addition, we clarified that the acetyltransferase activity of Eco1 is essential for its function. Our study thus identified a molecular target for the acetyltransferase Eco1 and revealed that Smc3 acetylation is a conserved mechanism in regulating sister chromatid cohesion.</P>

Electrospun non-leaching biocombatible antimicrobial cellulose acetate nanofibrous mats

Rong Li,Xuehong Ren,Qianyuan Jiang,Zhiwei Xie,Tung-Shi Huang 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.27 No.-

In this study cellulose acetate (CA) was modified with 4,40-diphenylmethane diisocyanate (MDI, an Nhalamineprecursor) in solution to form chemical bonds between CA and MDI to avoid the potentialrelease of biocides from CA. Nanofibers were obtained by electrospinning the CA–MDI solution. Theobtained CA–MDI nanofibers were characterized by FT-IR, SEM, TGA and DSC. TGA and DSC dataindicated that the CA–MDI nanofibers before and after chlorination had good thermal stability comparedwith CA nanofibers. 58% of active chlorine was lost after 2 h of UVA irradiation, and most of the activechlorine was lost after 4 h of UVA irradiation. However, about 85% of the chlorine could be regeneratedby rechlorination after 24 h of UVA irradiation. The active chlorine of the chlorinated nanofibers releasedslowly in water. The chlorinated CA–MDI nanofibers showed excellent antimicrobial properties againstEscherichia coli O157:H7 and Staphylococcus aureus within brief contact times. Also, the chlorinated CA–MDI nanofibers showed good biocompatibility.

Dark Septate Endophyte (DSE) Fungi Isolated from Metal Polluted Soils: Their Taxonomic Position, Tolerance, and Accumulation of Heavy Metals In Vitro

Yujie Zhang,Yan Zhang,Maojun Liu,Xiaodong Shi,Zhiwei Zhao 한국미생물학회 2008 The journal of microbiology Vol.46 No.6

To understand the possible role of the plant root associated fungi on metal tolerance, their role in the uptake of heavy metals and the potential transfer of these metal ions to the plant, three strains of dark septate endophytic (DSE) fungi were isolated from a waste smelter site in southwest China, and one strain was isolated from a non-contaminated site. According to molecular phylogenetic analysis of the ITS 1-5.8S rDNA-ITS 2 gene regions and morphological characteristics, one is identified as Exophiala pisciphila, and the other three are non-sporulating fungi under the experiment condition with the nearest phylogenetic affinities to the Thysanorea papuana strain EU041814. Tolerance and accumulation abilities of the three DSE strains for metals were investigated in liquid culture. Minimum inhibitory concentrations (MIC) of Pb, Zn, and Cd were determined. It was demonstrated that the tolerance of the DSE strains varied between metal species and strains. The E. pisciphila strain is able to accumulate lead and cadmium over 20% and 5% of dry weight of biomass, respectively. Partial of the sequestrated metals can be washed with CaCl2. Morphological and enzyme activity changes taking place in the presence of excessive Pb, Cd, and/or Zn also indicate that the mechanism of heavy metal tolerance and accumulation of the DSE strains would be a complex process. The findings indicated promising tolerance and accumulation of the DSE strains with potential values in metal cycling and restoration of soil and water system.