http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Increased Indigoid Accumulation by Plant Defense Activators in Polygonum tinctorium Lour.
Jin, Zhehao,Kim, Jin-Hee,Kim, Kwan-Su,Park, Sang-Un,Kim, Soo-Un The Korean Society for Applied Biological Chemisty 2012 Journal of Applied Biological Chemistry (J. Appl. Vol.58 No.3
Effect of plant defense activator on Polygonum tinctorium was evaluated to increase indigoid content in P. tinctorium. Three plant defense activators, 2,1,3-benzothiadiazole (BTH), 2,6-dichloroisonicotinic acid (INA), and acibenzolar-S methyl (ASM) were treated to two cultivars (Naju Local and Naram Blue) under the field condition at two different concentration regimens of 0.3 and 3 mM and harvested 7 days after treatment. The treatments increased indigo accumulation by 40 to 109% with statistical significance. Considering the treatment concentrations, BTH and INA at 0.3 mM were judged to be optimal with 2-fold increase in indigoid concentration. No statistical differences were found in the cultivars and maturity of leaves in the indigoid accumulation caused by plant activator treatment. This result is directly applicable to the P. tinctorium cultivation with high economic significance.
Jin Zhehao,Ro Dae-Kyun,Kim Soo-Un,Kwon Moonhyuk 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.2
Piperonal is a simple aromatic aldehyde compound with a characteristic cherry-like aroma and has been widely used in the flavor and fragrance industries. Despite piperonal being an important aroma in black pepper ( Piper nigrum ), its biosynthesis remains unknown. In this study, the bioinformatic analysis of the P. nigrum transcriptome identified a novel hydratase-lyase, displaying 72% amino acid identity with vanillin synthase, a member of the cysteine proteinase family. In in vivo substrate-feeding and in vitro enzyme assays, the hydratase-lyase catalyzed a side-chain cleavage of 3,4-methylenedioxycinnamic acid (3,4-MDCA) to produce 3,4-methylenedioxybenzaldehyde (piperonal) and thus was named piperonal synthase (PnPNS). The optimal pH for PnPNS activity was 7.0, and showed a K m of 317.2 μM and a k cat of 2.7 s −1 . The enzyme was most highly expressed in the leaves, followed by the fruit. This characterization allows for the implementation of PnPNS in various microbial platforms for the biological production of piperonal.
Assessment of Landslide Susceptibility using the PCA and ANFIS with Various Metaheuristic Algorithms
Zelu Chen,Hechun Quan,Ri Jin,Aifen Jin,Zhehao Lin,Guangri Jin,Guang-Zhu Jin 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.4
It is very important for the susceptibility assessment and disaster prediction of the region to effectively evaluate the landslide susceptibility. In this study, Particle Swarm Optimization (PSO), Artificial Bee Colony algorithm (ABC), Shuffled Frog Leaping Algorithm (SFLA) and Bat algorithm (BAT) are used to optimize Adaptive Neuro-Fuzzy Inference System (ANFIS) to evaluate the landslide susceptibility. 811 sample points were collected through remote sensing analysis and field investigation for susceptibility analysis. Fifteen landslide evaluation factors were quantified and normalized, and the Principal Component Analysis (PCA) method was used to compress them into 6 main factors. The accuracy analysis results of the area under the curve (AUC), Root Mean Square Error (RMSE) and Mean Absolute Error (MAE) evaluation models show that the AUC values of PSO, ABC, SFLA and BAT are 93.6%, 96.2%, 90.8% and 86.1%, respectively. Among them, the accuracy of ABC is the highest. This study effectively evaluates the landslide susceptibility through a new neural network hybrid method, which provides a theoretical basis for landslide disaster susceptibility management.
Characterization of a Piperonal Synthase in Pipper nigrum
Moonhyuk KWON,Zhehao JIN,Juraithip WUNGSINTAWEEKUL,Dae-Kyun RO,Seon-Won KIM,Soo-un KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Piperonal with a characteristic cherry-like aroma is a simple aromatic aldehyde compound, which has been widely used in flavor and fragrance industries. The aldehyde is also a crucial aroma-imparting molecule of black pepper (Piper nigrum). The bioinformatic analysis of P. nigrum transcriptome data predicted a hydratase-lyase with conserved cysteine proteinases in excess of 68% identity. The hydratase-lyase catalyzed specific side-chain cleavage of 3,4-methylenedioxycinnamic acid (3,4-MDCA) to produce 3,4-methylenedioxybenzaldehyde (piperonal) in vitro and in the gene-harboring yeast. The enzyme was named 3,4-MDCA hydratase-lyase (PnMCHL) or piperonal synthase. However, purported substrates such as cinnamic, coumaric, caffeic, ferulic, and piperic acids did not generate corresponding cleavage products in transgenic yeast. The optimal pH for in-vitro PnMHL was 7.0, and it had Km value for 317.2 μM and kcat for 2.7 s<SUP>-1</SUP>. The enzyme was highly expressed in the leaves, followed by fruits.
Hao Cui,He-Chun Quan,Ri Jin,Zhehao Lin 대한토목학회 2023 KSCE JOURNAL OF CIVIL ENGINEERING Vol.27 No.1
Flood susceptibility mapping is an important method for flood research. In this paper, we combine a backpropagation neural network (BPNN) with a genetic quantum algorithm (GQA) for the first time to develop flood susceptibility mapping. The area on the Chinese side of the Tumen River Basin was selected as the research object. A set of flood conditioning factors was selected based on relevant literature and an actual situation and then validated using the chi-square test and correlation analysis. Different weights were assigned using stepwise weight assessment ratio analysis. Finally, modeling and flood susceptibility mapping using GQA-BPNN. As a reference, the same work was performed with both the pure BPNN and optimized BPNN using a genetic algorithm (GA). The results show that the area under the curve, root mean squared error, Nash-Sutcliffe coefficient and percentage of bias are significantly better for the GQA-BPNN than for the BPNN and GA-BPNN and that the flood sensitivity maps constructed by the GQA-BPNN have more flood points in high flood sensitivity areas. Therefore, the GQA-BPNN method can be considered an effective method for flood susceptibility mapping.
Kang Min-Kyoung,Kim Ji-Young,Choi Young-Im,Hu Lujie,Yang Chaodong,Jin Zhehao,Park Yun Ji,Kim Soo-Un,Kim Sang-Min 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.4
Terpenoids are of great interests in a broad range of health-beneficial biological activities and various industrial applications. In plants, terpenoids are synthesized by two distinct pathways, methylerythritol phosphate (MEP) and mevalonate pathways in a separate location. MEP pathway supplies isoprene precursors isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP) of terpenoid biosynthesis in plant plastids. The MEP pathway has been an engineering target to increase the metabolic flux towards higher terpenoid production in plants. 1-Hydroxy-2-methyl-2-( E )-butenyl-4-diphosphate reductase (HDR) is the terminal step of the MEP pathway to regulate the terpenoid biosynthesis and is encoded by three paralogous genes in Ginkgo biloba. In this study, we assessed the effect of overexpression of GbHDR1 on terpenoid metabolism in poplar plants. Overexpression of GbHDR1 in poplar plants accelerated growth and delayed winter-bud formation. Transcript levels of gibberellin, chlorophylls, and carotenoid biosynthetic genes in GbHDR1- overexpressing ( GbHDR1 ox) poplars were up-regulated, suggesting metabolic flux enhancement. Moreover, enhanced contents of chlorophylls and carotenoids in the leaves of the GbHDR1 ox plants resulted in a higher photosynthetic rate as a consequence. Therefore, we expect the GbHDR1 overexpression will be a desirable engineering point of the MEP pathway for enhancing terpenoid metabolic flux and production in plants.