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Increased Indigoid Accumulation by Plant Defense Activators in Polygonum tinctorium Lour.
Jin, Zhehao,Kim, Jin-Hee,Kim, Kwan-Su,Park, Sang-Un,Kim, Soo-Un The Korean Society for Applied Biological Chemisty 2012 Journal of Applied Biological Chemistry (J. Appl. Vol.58 No.3
Effect of plant defense activator on Polygonum tinctorium was evaluated to increase indigoid content in P. tinctorium. Three plant defense activators, 2,1,3-benzothiadiazole (BTH), 2,6-dichloroisonicotinic acid (INA), and acibenzolar-S methyl (ASM) were treated to two cultivars (Naju Local and Naram Blue) under the field condition at two different concentration regimens of 0.3 and 3 mM and harvested 7 days after treatment. The treatments increased indigo accumulation by 40 to 109% with statistical significance. Considering the treatment concentrations, BTH and INA at 0.3 mM were judged to be optimal with 2-fold increase in indigoid concentration. No statistical differences were found in the cultivars and maturity of leaves in the indigoid accumulation caused by plant activator treatment. This result is directly applicable to the P. tinctorium cultivation with high economic significance.
Jin Zhehao,Ro Dae-Kyun,Kim Soo-Un,Kwon Moonhyuk 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.2
Piperonal is a simple aromatic aldehyde compound with a characteristic cherry-like aroma and has been widely used in the flavor and fragrance industries. Despite piperonal being an important aroma in black pepper ( Piper nigrum ), its biosynthesis remains unknown. In this study, the bioinformatic analysis of the P. nigrum transcriptome identified a novel hydratase-lyase, displaying 72% amino acid identity with vanillin synthase, a member of the cysteine proteinase family. In in vivo substrate-feeding and in vitro enzyme assays, the hydratase-lyase catalyzed a side-chain cleavage of 3,4-methylenedioxycinnamic acid (3,4-MDCA) to produce 3,4-methylenedioxybenzaldehyde (piperonal) and thus was named piperonal synthase (PnPNS). The optimal pH for PnPNS activity was 7.0, and showed a K m of 317.2 μM and a k cat of 2.7 s −1 . The enzyme was most highly expressed in the leaves, followed by the fruit. This characterization allows for the implementation of PnPNS in various microbial platforms for the biological production of piperonal.
Characterization of a Piperonal Synthase in Pipper nigrum
Moonhyuk KWON,Zhehao JIN,Juraithip WUNGSINTAWEEKUL,Dae-Kyun RO,Seon-Won KIM,Soo-un KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Piperonal with a characteristic cherry-like aroma is a simple aromatic aldehyde compound, which has been widely used in flavor and fragrance industries. The aldehyde is also a crucial aroma-imparting molecule of black pepper (Piper nigrum). The bioinformatic analysis of P. nigrum transcriptome data predicted a hydratase-lyase with conserved cysteine proteinases in excess of 68% identity. The hydratase-lyase catalyzed specific side-chain cleavage of 3,4-methylenedioxycinnamic acid (3,4-MDCA) to produce 3,4-methylenedioxybenzaldehyde (piperonal) in vitro and in the gene-harboring yeast. The enzyme was named 3,4-MDCA hydratase-lyase (PnMCHL) or piperonal synthase. However, purported substrates such as cinnamic, coumaric, caffeic, ferulic, and piperic acids did not generate corresponding cleavage products in transgenic yeast. The optimal pH for in-vitro PnMHL was 7.0, and it had Km value for 317.2 μM and kcat for 2.7 s<SUP>-1</SUP>. The enzyme was highly expressed in the leaves, followed by fruits.
배문기(Moonki Bae),윤철호(Zhehao Yin),김양수(Yangsu Kim),이기동(Keedong Lee),김영석(Youngsuk Kim) 한국자동차공학회 2009 한국자동차공학회 학술대회 및 전시회 Vol.2009 No.11
The CTBA(Coupled torsion beam axle) of automotive rear chassis parts connects to trailing arm which assembled on the rear axle by whelding process and achieves a task of absorbing torsion from wheel when automobile pass through the curves. In the welding process between CTBA and trailing arm, the welding deformation of trailing arm which connected to wheel hub axle module occurs, and it can effect to tow angle, camber angle, and caster of the wheel axle. Thus, the welding deformation should be controled strictly. In this paper, the welding process between CTBA and trailing arm was analyzed by FEM(Finite Element Method), the effect of welding time, temperature after welding process, clamping time and cooling time was grasped, and phase transformation after welding process was analyzed to minimize the after-machining(milling) of the welding part.
Li Xiaoyu,Zhang Zhehao,Zou Youlan,Li Zhaoyang,Duan Jinliang,Long Bo 한국탄소학회 2020 Carbon Letters Vol.30 No.5
We reported the synthesis of dendrite-like carbon nanotube-confned polymeric sulfur composite by modifying the surface of carbon nanotubes (CNTs) with trithiocyanuric acid (TTCA) and then copolymerizing with sulfur. DSC results show the successfully formation of robust chemical bonds between sulfur and TTCA modifed CNTs, which efectively avoid the dis-solution of polysulfde when used as cathodes for lithium–sulfur batteries. The composite with a high sulfur content of 78 wt% exhibits an initial charge capacity of 698 mAh g−1 and the residual capacity of 553 mAh g−1 after 1000 cycles at a rate of 1 C.
Park, Jihye,Lee, Minah,Feng, Dawei,Huang, Zhehao,Hinckley, Allison C.,Yakovenko, Andrey,Zou, Xiaodong,Cui, Yi,Bao, Zhenan American Chemical Society 2018 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.140 No.32
<P>Redox-active organic materials have gained growing attention as electrodes of rechargeable batteries. However, their key limitations are the low electronic conductivity and limited chemical and structural stability under redox conditions. Herein, we report a new cobalt-based 2D conductive metal-organic framework (MOF), Co-HAB, having stable, accessible, dense active sites for high-power energy storage device through conjugative coordination between a redox-active linker, hexaaminobenzene (HAB), and a Co(II) center. Given the exceptional capability of Co-HAB for stabilizing reactive HAB, a reversible three-electron redox reaction per HAB was successfully demonstrated for the first time, thereby presenting a promising new electrode material for sodium-ion storage. Specifically, through synthetic tunability of Co-HAB, the bulk electrical conductivity of 1.57 S cm<SUP>-1</SUP> was achieved, enabling an extremely high rate capability, delivering 214 mAh g<SUP>-1</SUP> within 7 min or 152 mAh g<SUP>-1</SUP> in 45 s. Meanwhile, an almost linear increase of the areal capacity upon increasing active mass loading up to 9.6 mg cm<SUP>-2</SUP> was obtained, demonstrating 2.6 mAh cm<SUP>-2</SUP> with a trace amount of conducting agent.</P> [FIG OMISSION]</BR>
Hao Cui,He-Chun Quan,Ri Jin,Zhehao Lin 대한토목학회 2023 KSCE JOURNAL OF CIVIL ENGINEERING Vol.27 No.1
Flood susceptibility mapping is an important method for flood research. In this paper, we combine a backpropagation neural network (BPNN) with a genetic quantum algorithm (GQA) for the first time to develop flood susceptibility mapping. The area on the Chinese side of the Tumen River Basin was selected as the research object. A set of flood conditioning factors was selected based on relevant literature and an actual situation and then validated using the chi-square test and correlation analysis. Different weights were assigned using stepwise weight assessment ratio analysis. Finally, modeling and flood susceptibility mapping using GQA-BPNN. As a reference, the same work was performed with both the pure BPNN and optimized BPNN using a genetic algorithm (GA). The results show that the area under the curve, root mean squared error, Nash-Sutcliffe coefficient and percentage of bias are significantly better for the GQA-BPNN than for the BPNN and GA-BPNN and that the flood sensitivity maps constructed by the GQA-BPNN have more flood points in high flood sensitivity areas. Therefore, the GQA-BPNN method can be considered an effective method for flood susceptibility mapping.