Phosphorylation of DYNLT1 at Serine 82 Regulates Microtubule Stability and Mitochondrial Permeabilization in Hypoxia

Xue Xu,Yue-sheng Huang,Qiong Zhang,Jiong-yu Hu,Dong-xia Zhang2,Xu-pin Jiang,jie-zhi Jia,Jing-ci Zhu 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.4

Hypoxia-induced microtubule disruption and mitochondrial permeability transition (mPT) are crucial events leading to fatal cell damage and recent studies showed that microtubules (MTs) are involved in the modulation of mitochondrial function. Dynein light chain Tctex-type 1 (DYNLT1) is thought to be associated with MTs and mitochondria. Previously we demonstrated that DYNLT1 knockdown aggravates hypoxia-induced mitochondrial permeabilization, which indicates a role of DYNLT1 in hypoxic cytoprotection. But the underlying regulatory mechanism of DYNLT1 remains illusive. Here we aimed to investigate the phosphorylation alteration of DYNLT1 at serine 82 (S82) in hypoxia (1% O2). We therefore constructed recombinant adenoviruses to generate S82E and S82A mutants, used to transfect H9c2 and HeLa cell lines. Development of hypoxia-induced mPT (MMP examining, Cyt c release and mPT pore opening assay), hypoxic energy metabolism (cellular viability and ATP quantification), and stability of MTs were examined. Our results showed that phosph-S82 (S82-P) expression was increased in early hypoxia; S82E mutation (phosphomimic) aggravated mitochondrial damage, ele-vated the free tubulin in cytoplasm and decreased the cellular viability; S82A mutation (dephosphomimic) seemed to diminish the hypoxia-induced injury. These data suggest that DYNLT1 phosphorylation at S82 is involved in MTs and mitochondria regulation, and their interaction and cooperation contribute to the cellular hypoxic tolerance. Thus, we provide new insights into a DYNLT1 mechanism in stabilizing MTs and mitochondria, and propose a potential therapeutic target for hypoxia cytoprotective studies.

Longitudinal Intrinsic Brain Activity Changes in Cirrhotic Patients before and One Month after Liver Transplantation

Yue Cheng,Li-Xiang Huang,Li Zhang,Ming Ma,Shuang-Shuang Xie,Qian Ji,Xiao-Dong Zhang,Gao-Yan Zhang,Xue-Ning Zhang,Hong-Yan Ni,Wen Shen 대한영상의학회 2017 Korean Journal of Radiology Vol.18 No.2

Objective: To evaluate the spontaneous brain activity alterations in liver transplantation (LT) recipients using resting-state functional MRI. Materials and Methods: Twenty cirrhotic patients as transplant candidates and 25 healthy controls (HCs) were included in this study. All patients repeated the MRI study one month after LT. Amplitude of low-frequency fluctuation (ALFF) values were compared between cirrhotic patients (both pre- and post-LT) and HCs as well as between the pre- and post-LT groups. The relationship between ALFF changes and venous blood ammonia levels and neuropsychological tests were investigated using Pearson’s correlation analysis. Results: In the cirrhotic patients, decreased ALFF in the vision-related regions (left lingual gyrus and calcarine), sensorimotor-related regions (left postcentral gyrus and middle cingulate cortex), and the default-mode network (bilateral precuneus and left inferior parietal lobule) were restored, and the increased ALFF in the temporal and frontal lobe improved in the early period after LT. The ALFF decreases persisted in the right supplementary motor area, inferior parietal lobule, and calcarine. The ALFF changes in the right precuneus were negatively correlated with changes in number connection test-A scores (r = 0.507, p < 0.05). Conclusion: LT improved spontaneous brain activity and the results for associated cognition tests. However, decreased ALFF in some areas persisted, and new-onset abnormal ALFF were possible, indicating that complete cognitive function recovery may need more time.

Magnesium isoglycyrrhizinate inhibits L-type Ca2+ channels, Ca2+ transients, and contractility but not hERG K+ channels

Yue Lin,Yuanyuan Zhang,Qiongtao Song,Tao Song,Xue Han,Ying Zhang,Xuan Zhang,Xi Chu,Fenghua Zhang,Li Chu,Jianping Zhang 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.10

To explore the cardiovascular protective effectsof Magnesium isoglycyrrhizinate (MI), especially theunderlying cellular mechanisms related to L-type calciumchannels and myocardial contractility, and to examine theeffects of MI on hERG K? current expressed in HEK293cells. We used the whole-cell patch clamp technique,video-based edge detection and dual excitation fluorescencephotomultiplier systems to explore the effect of MIon L-type Ca2? currents (ICa-L) and cell contraction in ratcardiomyocytes. We also examined the rapidly activatingdelayed rectifier potassium current (IKr) expressed inHEK293 cells using a perforated patch clamp. MI inhibitedICa-L in a dose-dependent manner, with a half-maximalinhibitory concentration (IC50) of 0.22 mg/ml, and themaximal inhibitory effect was 61.10 ± 0.59%. MI at aconcentration of 0.3 mg/ml reduced cell shortening by24.12 ± 3.97% and the peak value of the Ca2? transient by36.54 ± 4.96%. MI had no significant influence on hERGK? channels expressed in HEK293 cells at all testpotentials. MI exerts protective effects on the heart via theinhibition of ICa-L and cell shortening in rat cardiomyocytes. However, MI had no significant influence on IKr;thus, MI may exert cardioprotective effects without causingdrug-induced long QT syndrome.

Critical effects of long non-coding RNA on fibrosis diseases

Yue Zhang,Gang Luo,Yi Zhang,Mengjie Zhang,Jian Zhou,Weiwu Gao,Xiuyun Xuan,Xia Yang,Di Yang,Zhiqiang Tian,Bing Ni,Jun Tang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

The expression or dysfunction of long non-coding RNAs (lncRNAs) is closely related to various hereditary diseases, autoimmune diseases, metabolic diseases and tumors. LncRNAs were also recently recognized as functional regulators of fibrosis, which is a secondary process in many of these diseases and a primary pathology in fibrosis diseases. We review the latest findings on lncRNAs in fibrosis diseases of the liver, myocardium, kidney, lung and peritoneum. We also discuss the potential of diseaserelated lncRNAs as therapeutic targets for the clinical treatment of human fibrosis diseases.

Design of a Light and Small Dual-band Airborne Despun Optical System

Luqing Zhang,Ning Zhang,Xiping Xu,Kailin Zhang,Yue Zhang,Jiachong Li 한국광학회 2024 Current Optics and Photonics Vol.8 No.1

In aerial cameras, image quality is easily affected by weather, temperature, and the attitude of the aircraft. Aiming at this phenomenon, based on the theory of two-step zoom optical systems, a dual-band optical-despun two-step zoom optical system is designed. The system has a small field of view of 2.00° × 1.60°, and a large field of view of 4.00° × 3.20°. In the zoom process, the wavelength range is 0.45– 0.70 μm and 0.75–1.10 μm, and the size of the optical system is 168 mm (L) × 90 mm (W) × 60 mm (H). The overall lens weight is only 170.8 g, which has advantages for miniaturization and light weight. At the Nyquist frequency of 104 lp/mm, the modulation transfer function of the visible-light optical system is more than 0.44, and that of the near-infrared optical system is more than 0.30, both of which havegood imaging quality and tolerance characteristics in the range of −45 to 60 ℃.

Investigation of Eco-friendly Dyeing of Para-aramid Using Supercritical CO2

Yue Zhang,Huanda Zheng,Laijiu Zheng,Tao Cai,Fuer Zheng 한국섬유공학회 2022 Fibers and polymers Vol.23 No.8

In order to solve the problems of dyeing difficulty and serious pollution of traditional water-based dyeing of paraaramid,supercritical CO2 fluid was employed as a dyeing solvent to dye para-aramid yarn (temperatures 80-120 ℃, pressures16-24 MPa, dyeing time 15-90 min, and dye concentration 1 %-7 % o.m.f.). The K/S values reached 2.07 and 1.91 at 120 ℃,24 MPa, 60 min, 5 % o.m.f., and a CO2 flow of 20 g/min with C. I. Disperse Red 54 and C. I. Disperse Blue 79, respectively. The results proved that para-aramid yarn could be dyed in supercritical CO2 fluid. Moreover, the thermal and mechanicalproperties of dyed para-aramid yarns in supercritical CO2 showed a slightly increasing trend. The washing, rubbing, and thelight color fastness of the dyed samples were rated above 4-5, and the color fastness durability of the dyed para-aramid wasalso presented good.

다산역학에서 象數와 義理의 통합적 경향

ZHANG YUE 한국유교학회 2018 유교사상문화연구 Vol.0 No.74

Neo - Confucianism was first introduced in Goryeo, and when Korea was founded, it became the founding ideology. But later on, as the Neo - Confucianism gradually lost its original spirit and vitality, it became obsolete and became conservative. Neo-Confucianism, which emphasizes the Yili(義理), has lost its status as a leading orthodoxist of human autonomy, and is no longer responsible for solving social problems. At this time, Practical learning was born, claiming to return to the Confucian classics by paying attention to real problems such as economy and institutions. As a synthesizer, Tasan based on reality and actual situation, and as a result, he established a unique ideology. In Tasan's interpretation of "Zhouyi", Tasan criticized and based on the interpretation of "Zhouyi" by the scholars of the past, suggestting the interpretation method of Yilisifa(易理四法). At the same time, Tasan broke the boundaries of traditional Xiangshu(象數) and Yili(義理), and acknowledged that "Zhouyi" had a dual nature as a book of divination and Self-cultivation. The viewpoint is that Tasan regarded divination as a tool, and the ultimate purpose of "Zhouyi" is to "asking for the command of heaven and conforming to his will", which aimed to change the error to good. This has injectted humanistic rationality into "Zhouyi", and Combined Xiangshu and Yili. Therefore, in order to demonstrate this characteristic of Tasan’s thought of "Zhouyi", this paper focuses on the emphasis of textual research and the critical acceptance of the interpretation of "Zhouyi" by the scholars in the past. In addition, by examining Tasan’s new view and the interpretation of the 64 gua(卦), explored Tasan’s unique viewpoint of regarding "Zhouyi" as a book of divination and Self-cultivation. 성리학은 고려에 처음 도입되었고, 조선이 건국될 때 建國이념이 되었다. 그러나 이후에는 성리학이 점점 원래의 정신과 활력을 상실해 버려 陳腐해지고 保守적으로 되어 형식과 관념에 빠져 玄虛한 학문으로 되어 버림에 따라 그 폐단이 날이 갈수록 심해졌다. 義理를 강조하는 성리학은 사람의 自律性에 대한 주도적 역할을 하는 정통 사상으로서의 지위를 잃게 되었을 뿐더러 사회 문제를 해결하는 역할을 더 이상 담당하지 못하게 되었다. 이 때 禮學과 義理를 중시하는 성리학에서 벗어나 경제와 제도 등 현실 문제에 대해 관심을 기울이고 儒家의 經典으로 복귀하기를 주장하는 실학파가 탄생하였다. 다산은 실학파의 집대성자로서 현실에 입각하여 실제 상황에 맞는 전통에 기초하면서도 독창성 있는 사상체계를 만들었다. 이러한 학문적 특징은 『周易』에 대한 해석에도 잘 드러났다. 다산의 『周易』해석을 살펴보면, 다산은 역대 학자들의 『周易』해석에 기초하여 비판하면서 계승하고 계승하면서 비판함으로써 한대 상수역적 해석방법론을 중심으로 하는 易理四法을 제시하였다. 이와 동시에 다산이 전통적인 상수역학과 의리역학의 경계를 깨뜨리고, 『周易』이 卜筮의 책과 改過遷善의 책으로서의 二重的 성격을 가지고 있음을 인정하였다. 이러한 다산의 관점은 그가 卜筮를 도구로 삼고 성인들이 『周易』을 창조하는 최종 목적은 “하늘의 명을 청하여 그 뜻을 순응[請天之命而順其旨]”함으로써 改過遷善하는 데에 있다고 주장하는 것으로 알 수 있다. 이는 원래 卜筮의 책으로서의 『周易』에 도덕적 人文理性의 함의를 부가하여 象數와 義理를 통합시킨 경향이다. 그러므로 다산 역학사상의 이러한 특징을 논증하기 위해 본 논문은 考證學的 방법에 대한 重視와 易學史에서 諸家의 『周易』해석에 대한 批判的 수용의 두 측면을 통해 다산의 易學 발전사에 대한 辨證的 인식을 살펴보았다. 또한 역학체계의 새로운 구축과 64괘 괘효사에 대한 다산의 해석을 살펴봄으로써 『周易』의 성격에 대한 그의 二重的 인식을 검토하였다.

A native Trichoderma harzianum strain Th62 displays antagonistic activities against phytopathogenic fungi and promotes the growth of Celosia cristata

Yue-Feng Wang,Xue-Yue Hou,Chuan-Ying Jiang,Tong-Tong Zhai,Rui Miao,Jun-Jie Deng,Zhi-Hong Yao,Rongshu Zhang 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.2

Trichoderma spp. are widely applied, eco-friendly mycofungicides and plant growth promoters. Native Trichoderma strainsare likely to have more productive, stable biocontrol and biofertilizer activities since they are well adapted to the local environment. In this study, we isolated a native Trichoderma strain ‘Th62’ from the rhizosphere soil of wild Chelidonium majusplants in Harbin, China (126.6341°E, 45.7242°N). The isolated Trichoderma strain was identifi ed as a T. harzianum strainvia morphological observation and molecular methods based on the rDNA internal transcribed spacer region and elongationfactor-1α gene sequences. Signifi cant antagonistic activities of Th62 against fi ve soil-borne fungal phytopathogens,Fusarium oxysporum , Sclerotinia sclerotiorum , Alternaria alternata , Cytospora chrysosperma, and Rhizoctonia solani ,were confi rmed by dual-culture assays. Furthermore, the crude fermentation products of Th62 also displayed antifungalactivities against these fi ve pathogens. To evaluate the function of Th62 as a biofertilizer, we subsequently applied Th62on cockscomb ( Celosia cristata L), a plant species with both ornamental and medicinal values, by inoculation with Th62conidia at diff erent concentrations, 1 × 10 10 cfu mL −1 , 1 × 10 11 cfu mL −1 , and 1 × 10 12 cfu mL −1 . The benefi cial eff ects ofTh62 were evaluated by measuring the growth and photosynthetic traits of the inoculated cockscomb plants, and the resultsdemonstrated that Th62 signifi cantly improved the photosynthetic effi ciency, photosynthetic capacity, and the adaptabilityto intense light of the inoculated cockscomb plants compared to the controls. Consistently, Th62 inoculation signifi cantlyimproved the growth and fl ower yield of cockscomb. We presented a positive case of isolating and applying native microbialresources on local plantation practices.

Macrophages Promote Coal Tar Pitch Extract-induced Tumorigenesis of BEAS-2B Cells and Tumor Metastasis in Nude Mice Mediated by AP-1

Zhang, Peng,Jin, Yue-Fei,Zhang, Qiao,Wu, Yi-Ming,Wu, Wei-Dong,Yao, Wu,Wu, Yong-Jun,Li, Zhi-Tao,Zhao, Yong,Liu, Yu,Feng, Fei-Fei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background: We sought to evaluate the role of tumor associated macrophages (TAMs) on the promotion of coal tar pitch extract (CTPE)-induced tumorigenesis of human bronchial epithelial cells (BEAS-2B) and tumor metastasis in nude mice, and related mechanisms. Materials and Methods: BEAS-2B cells were first treated with 2.4 mg/mL CTPE for 72 hours. After removal of CTPE, the cells were continuously cultured and passaged using trypsin-EDTA. THP-1 cells were used as macrophage-like cells. BEAS-2B cells under different conditions (n=6/group) were injected into the back necks of nude mice, and alterations of tumor xenograft growth, indicative of tumorigenicity, and tumor metastasis were determined. Pathological changes (tumor nests and microvascular lesions) of HE-stained tumor tissues were also evaluated. The expression of AP-1(c-Jun) in xenografts and metastatic tumors was determined using immunohistochemistry. Results: Tumor size and weight in nude mice transplanted with the mixture of CTPE-induced passage 30 BEAS-2B and THP-1 cells (2:1) were increased compared to those from the CTPE-treated BEAS-2B cells at passage 30 alone at different observation time points. Tumor metastasis to lymph nodes and liver was only detected after transplantation of a mixture the two kinds of cells. The numbers of tumor nests and microvascular lesions, and the expression levels of AP-1 (c-Jun) in tumors from the mixture of two kinds of cells were increased apparently in contrast to those in tumor from the CTPE-treated BEAS-2B cells of passage 30 alone. In addition, there was positive correlation between AP-1 (c-Jun) expression level and the number of microvascular lesions, or between AP-1 (c-Jun) expression level and tumor metastasis in these two groups. Conclusions: TAMs not only facilitate tumorigenesis transformation of CTPE-induced BEAS-2B cells, but also promote tumor growth, angiogenesis and metastasis in nude mice in vivo, which may be mediated by AP-1.

A Feature-Based Malicious Executable Detection Approach Using Transfer Learning

( Yue Zhang ),( Hyun-hoyang ),( Ning Gao ) 한국인터넷정보학회 2020 인터넷정보학회논문지 Vol.21 No.5

At present, the existing virus recognition systems usually use signature approach to detect malicious executable files, but these methods often fail to detect new and invisible malware. At the same time, some methods try to use more general features to detect malware, and achieve some success. Moreover, machine learning-based approaches are applied to detect malware, which depend on features extracted from malicious codes. However, the different distribution of features oftraining and testing datasets also impacts the effectiveness of the detection models. And the generation oflabeled datasets need to spend a significant amount time, which degrades the performance of the learning method. In this paper, we use transfer learning to detect new and previously unseen malware. We first extract the features of Portable Executable (PE) files, then combine transfer learning training model with KNN approachto detect the new and unseen malware. We also evaluate the detection performance of a classifier in terms of precision, recall, F1, and so on. The experimental results demonstrate that proposed method with high detection rates andcan be anticipated to carry out as well in the real-world environment.