Low Microsomal Epoxide Hydrolase Expression is Associated with Bladder Carcinogenesis and Recurrence

Zhang, Zhe,Yu, Xiu-Yue,Zhang, Guo-Jun,Guo, Kun-Feng,Kong, Chui-Ze Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

Microsomal epoxide hydrolase (mEH) plays a significant role in the metabolism of numerous xenobiotics and is associated with several forms of cancer. Here, we investigated the role of mEH expression in bladder carcinogenesis, subsequent progression and recurrence. The expression of mEH was analyzed by Western blot in 50 bladder urothelial carcinoma and 20 normal epithelial tissues. There was a significantly higher mEH expression in the normal epithelium (P<0.05) and mEH expression was lower in high stage than in low stage tumors (P<0.05). Further, immunohistochmistry in 106 bladder urothelial carcinoma demonstrated mEH expression to be negatively correlated with histological grade, pT stage and recurrence (P<0.05). These findings suggest the important role of mEH in bladder carcinogenesis, cancer development and recurrence, providing support for efforts to develop mEH-based gene therapy.

Application of N-substituted (aminomethyl)benzoate Strategy in Design of Scutellarein Derivatives with Improved Caco-2 Cell Permeability and In Vitro Antioxidative Activity

Ze-Qin Dai,Hang Su,Min Luo,Yu Ou,Xiao-Zhong Fu,Yong-Xi Dong,Yu-Feng Cha,Shun Zhang,Yong Huang,Yong-Lin Wang 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.8

A series of 4′-N-substituted (aminomethyl)benzoate derivatives of scutellarein were designed and synthesized. Evaluation of their physiochemical properties showed that the designed target compounds 5a–e exhibit higher chemical stability and aqueous solubility than scutellarin and scutellarein. The permeability (Papp AP to BL ) of 5c–e in Caco-2 cells were 2.8, 8.1, and 12.6 times higher than that of scutellarin and 1.3, 4.1, and 6.0 times higher than that of scutellarein; especially, 5e had the highest P app AP to BL value (7.19 ± 0.31 × 10−6 cm/s) and the lowest ER (P app BL to AP /P app AP to BL ) value of 0.17. In vitro antioxidative evaluation results revealed that 5e could protect against H2O2 -induced PC12 cells’ oxidative damage by attenuating mitochondrial membrane potential loss and decreasing H2O2 -induced reactive oxygen species (ROS) production.

Estimation and compensation for deviation of contour in slow tool servo precision turning for complicated curved surface

Feng-Ze Qin,Jian-Wei Ma,Zhen-Yuan Jia,Guan-Lin Li,Jian-Zhou Zhang 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.8

In the presence of extremely high precision turning the complicated curved surface with geometry features of large slope gradient and multi convex-concave characteristics, the micron-scale deviation of contour resulting from the tracking deviation of the feed axis becomes non-negligible. To diminish the micron-scale contouring deviation, this study presents a valid contouring error estimation and pre-compensation method for slow tool servo (STS) precision turning. Aiming at estimating the tracking deviation of the axis of the turning machine tool in the machining process, the steady-state tracking deviation model of the axis is first constructed. The proposed multiply tangential estimation method is derived from the Taylor series of the foot point. Since the rotation feed movement of the C axis is the main movement in cutting forming movement for STS turning machining, the monotonicity of the displacement for the C axis is ensured. The compensation value for the axis position is derived through the Jacobi matrix of the STS turning machine. With the presented approach, the tool tip location deviation induced by the tracking deviation of the feed axis reduces from 1.087 μm to 0.025 μm. The measurement results of the comparison experiments indicate that with the presented method in this study, the contouring error of the machined part reduces from 5.72 μm to 4.36 μm. The micron-scale contouring error resulting from the tracking deviation of the feed axis is effectually compensated.

Comprehensive Expression Analysis Suggests Functional Overlapping of Human FOX Transcription Factors in Cancer

Zhang, Ya-Li,Sun, Feng-Ting,Zhang, Ze,Chen, Xiao-Xu,Liu, Ai-Xiang,Pan, Jing-Jing,Peng, Fei,Zhou, Shuai,Sun, Li-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Forkhead-box (FOX) transcription factors comprise a large gene family that contains more than 50 members in man. Extensive studies have revealed that they not only have functions in control of growth and development, but also play important roles in different diseases, especially in cancer. However, biological functions for most of the members in the FOX family remain unknown. In the present study, the expression of 39 FOX genes in 48 kinds of cancer was mined from the Gene Expression Atlas database of European Bioinformatics Institute. The analysis results showed that some FOX genes demonstrate overlapping expression in various cancers, which suggests particular biological functions. The pleiotropic features of the FOX genes make them excellent candidates in efforts aimed to give medical treatment for cancers at the genetic level. The results also indicated that different FOX genes may have the synergy or antagonistics effects in the same cancers. The study provides clues for further functional analysis of FOX genes, especially for the pleiotropic biological functions and crosstalk of FOX genes in human cancers.

Cloning, characterization, and RNAi effect of the chitin synthase B gene in Cnaphalocrocis medinalis

Zhang Ze-Jie,Xia Lang,Du Juan,Li Shang-Wei,Zhao Feng 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1

The rice leaf folder (RLF), Cnaphalocrocis medinalis, is one of the major pests of rice, and chitin synthase is a key enzyme for the chitin synthesis pathway in insects. In this study, the chitin synthase B gene from C. medinalis (CmCHSB) was cloned and characterized. The cDNA of CmCHSB is 4824 bp in length, containing an open reading frame of 4578 nucleotides that encodes 1525 amino acids. The CmCHSB zymoprotein consists of 10 trans membrane domains (TMDs) in the N-terminus, a middle conserved catalytic domain, and 7 TMDs in the C-ter minus. Homology and phylogenetic analyses revealed that CmCHSB possesses the closest relationship with its homolog in Ostrinia furnacalis. CmCHSB was expressed throughout development and in all of the adult tissues tested, with the highest expression level in the adult and in the midgut. Silencing of CmCHSB through RNA interference (RNAi) severely affected RLF larval growth and caused larval lethality. Our results revealed that CmCHSB is essential for RLF growth and development, which sheds new light on the characteristics and functions of this gene. These findings will be helpful for green control of RLF, by targeting the CmCHSB gene using RNAi technology.

Practical Calculation Method for the Short‑Circuit Current of Power Grids with High Temperature Superconducting Fault Current Limiters

Hai‑feng Hong,Meng‑ze Yu,Xiao‑hui Zhang,Jia‑jia Huan,Yu Sui,Xian‑xian Pan 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.2

With the expansion of the power grid scale and the enhancement of network structure interconnection, the short-circuit current of power grids is increasingly close to the upper capacity limit of circuit breakers. The fault current limiter is efective in suppressing the short-circuit current of power grids, and with the mature development of superconducting technology, high-temperature superconducting fault current limiters (HTS-FCLs) have been widely used in power grids. Due to the nonlinear impedance–current characteristic of HTS-FCLs, the traditional short-circuit current calculation method is inapplicable to power grids with HTS-FCLs. First, based on the establishment of the electromechanical transient model and the short-circuit calculation model, the short-circuit current calculation procedure for power grids with HTS-FCLs is proposed. Second, the calculation methods for three-phase and single-phase short-circuit currents at the substation bus in power grids with HTS-FCLs are analyzed. Finally, three-phase and single-phase short-circuit currents at the 500 kV Xijiang substation bus in the Guangdong power grid are calculated as the study case. Simulation results show that compared with the situation where there is no HTS-FCL installed, the short-circuit current on the 500 kV bus decreases from 65.34 to 58.31 kA in terms of the three-phase short-circuit fault, and decreases from 54.56 to 46.62 kA in terms of the single-phase short-circuit fault, thus verifying the efectiveness and practicability of HTS-FCLs in suppressing the short-circuit current, which is signifcant for the safe, stable and reliable operation of the power grid.

Relative Photonic Properties of Fe/TiO2-Nanocarbon Catalysts for Degradation of MB Solution under Visible Light

오원춘,Feng-Jun Zhang,Ze-Da Meng,Kan Zhang 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.5

Nanocarbon supported Fe/TiO2 composite catalysts were prepared using CNTs (carbon nanotubes) and C60 (fullerene)as nanocarbon sources by a modified sol-gel method. The Fe/TiO2-nanocarbon composites were characterized by the BET surface area, scanning electron microscope (SEM), Transmission Electron Microscope (TEM), X-ray diffraction (XRD), energy dispersive X-ray (EDX) and UV-vis spectra. In comparison with non-nanocarbon doped Fe/TiO2 composites,the nanocarbon supported Fe/TiO2 composites had higher absorption ability with a larger specific surface area, and showed higher photocatalytic activity during the degradation of methylene blue (MB) under visible light. The reasons for the obvious increase of photocatalytic activity indicated that the photoactivity not only benefits from nanocarbon introduced, but also relates to the cooperative effect of the Fe as a dopant.

MuDeepNet: Unsupervised Learning of Dense Depth, Optical Flow and Camera Pose Using Multi-view Consistency Loss

Jun-Ning Zhang,Qun-Xing Su,Peng-Yuan Liu,Hong-Yu Ge,Ze-Feng Zhang 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.10

We take formulate structure from motion as a learning problem, and propose an end-to-end learning framework to calculate the image depth, optical flow, and the camera motion. This framework is composed of multiple encoder-ecoder networks. The key part of the network structure is the FlowNet, which can improve the accuracy of the estimated camera ego-motion and depth. As with recent studies, we use an end-to-end learning approach with multi-view synthesis as a variety of supervision, and proposes multi-view consistency losses to constrain both depth and camera ego-motion, requiring only monocular video sequences for training. Compared to the recently popular depth-estimation-networks using a single image, our network learns to use motion parallax correction depth. Although MuDeepNet training requires the use of two adjacent frames to obtain motion parallax, it is tested by using a single image. Thus, MuDeepNet is a monocular system. The experiments on KITTI dataset show our MuDeepNet outperforms other methods.

Prognostic Role of Nucleophosmin in Colorectal Carcinomas

Yang, Yu-Feng,Zhang, Xi-Ying,Yang, Mei,He, Ze-Hua,Peng, Ning-Fu,Xie, Shu-Rui,Xie, Yan-Fang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Aim: Recent research suggests that nucleophosmin (NPM) may be a prognostic marker in colorectal carcinomas (CRC). We here tested its use to predict the survival of CRC patients. Methods: We investigated NPM expression by immunohistochemistry in histologically normal to malignant colorectal tissues and evaluated its association with clinicopathological variables. Overall and disease-free survival after tumor removal were calculated by the Kaplan-Meier method, and differences in survival curves were analyzed by the log-rank test. The Cox proportional hazards model was used for multivariate analysis of prognostic factors. Results: NPM expression was found significantly upregulated in CRC compared to adjacent colorectal tissue, villous adenoma, tubular adenoma and normal colorectal mucosa (p<0.05 for all). NPM expression was statistically linked to cancer embolus, lymph node metastasis, differentiation grade, and recurrence of CRC. Overall and disease-free survival of NPM-negative CRC patients tended to be better than those for patients with NPM-positive lesions (log-rank statistic, p<0.05 for all). Multivariate analysis indicated NPM expression as an independent prognostic indicator for CRC patients (p<0.05 ). Conclusion: Our results suggest that NPM expression can predict the survival of CRC patients. Prognosis of CRC is determined by not only many known prognostic factors but also by NPM expression.

Variation of Urinary and Serum Trace Elements (Ca, Zn, Cu, Se) in Bladder Carcinoma in China

Guo, Kun-Feng,Zhang, Zhe,Wang, Jun-Yong,Gao, Sheng-Lin,Liu, Jiao,Zhan, Bo,Chen, Zhi-Peng,Kong, Chui-Ze Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Backgrounds: Deficiency or excess of trace elements can induce body metabolic disorders and cellular growth disturbance, even mutation and cancerization. Since there are few studies of the effect of trace elements in bladder carcinoma in China, the aim of this study was thus to assess variation using a case control approach. Methods: To determine this, 81 patients with bladder carcinoma chosen as a study group and 130 healthy persons chosen as a control group were all assayed for urinary and serum trace elements (calcium [Ca], zinc [Zn], copper [Cu], selenium [Se]) using an atomic absorption spectrophotometer, and the results were analyzed by independent sample t tests. The correlative factors on questionnaires answered by all persons were analyzed by logistic regression. Results: The results showed urinary Ca, Zn and serum Cu levels of the study group to be significantly higher (P<0.05) than those of he control group. Serum Ca and Se levels of study group were significantly lower (P<0.05) than those of control group. Conclusion: There were higher urinary Zn and serum Cu concentrations in bladder carcinoma cases. Bladder carcinoma may be associated with Ca metabolic disorder, leading to higher urinary Ca and lower serum Ca. Low serum Se and smoking appear to be other risk factors for bladder carcinoma in China.