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In planta production and characterization of full-length human adipose triglyceride lipase
Zaikova Kseniia,정진성,김경록,구강모,김정선,이동욱 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.6
Adipose triglyceride lipase (ATGL) is an enzyme that plays a critical role in the mobilization of stored triacylglycerol. Despite its importance in lipid metabolism, in-depth biochemical characterization of ATGL has been limited because the expression and purifcation of this enzyme are not feasible. In this study, we attempted to generate the full-length human ATGL in Nicotiana benthamiana. The ATGL expressed in N. benthamiana was highly soluble and stable. The purifed ATGL exhibited lipase activity in vitro. In conclusion, the full-length human ATGL could be reliably produced using a plant-based expression system, thereby enabling detailed biochemical characterization of this enzyme.