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Toshiyo Tamura,Yutaka Kimura 대한의용생체공학회 2015 Biomedical Engineering Letters (BMEL) Vol.5 No.1
The forthcoming very elderly society threatens to render thehealth insurance system of Japan dysfunctional. To solve thisissue, a new screening and interventional program specificallytargeting metabolic syndrome commenced in April 2008. This program targeted individuals in the age group 40-74years. The program sought to prevent the risk of developmentof lifestyle-related diseases. In this review, we analyze 5-yearstatistical data, discuss the efficiency of the screeningprogram, and offer a brief explanation of the applicability ofinformation communication technology (ICT). Interventionwas effective to prevent metabolic syndrome based onphysiological parameters. Using ICT, a database has beencreated to collect both health checkup records and receipts. Computer- and Web-based health guidance has beenattempted but no reports thereon have yet been published. Further efforts should be made to improve the design, cost,and handling characteristics of both the software and hardware.
A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle
Hidetoshi Higuchi,Hidetomo Iwano,Kazuhiro Kawai,Takehiro Ohta,Tetsu Obayashi,Kazuhiko Hirose,Nobuhiko Ito,Hiroshi Yokota,Yutaka Tamura,Hajime Nagahata 대한수의학회 2011 Journal of Veterinary Science Vol.12 No.2
A simplified polymerase chain reaction (PCR) assay was developed for fast and easy screening of mycoplasma mastitis in dairy cattle. Species of major mycoplasma strains [Mycoplasma (M.) bovis, M. arginini, M. bovigenitalium, M. californicum, M. bovirhinis, M. alkalescens and M. canadense] in cultured milk samples were detected by this simplified PCR-based method as well as a standard PCR technique. The minimum concentration limit for detecting mycoplasma by the simplified PCR was estimated to be about 2.5 × 10^3 cfu/mL and was similar to that of the standard PCR. We compared the specificity and sensitivity of the simplified PCR to those of a culture method. Out of 1,685 milk samples cultured in mycoplasma broth, the simplified PCR detected Mycoplasma DNA in 152 that were also positive according to the culture assay. The sensitivity and specificity of the simplified PCR were 98.7% and 99.7%, respectively, for detecting mycoplasma in those cultures. The results obtained by the simplified PCR were consistent with ones from standard PCR. This newly developed simplified PCR, which does not require DNA purification, can analyze about 300 cultured samples within 3 h. The results from our study suggest that the simplified PCR can be used for mycoplasma mastitis screening in large-scale dairy farms.