수술실 손 소독제의 종류에 따른 균주 수의 변화

홍성윤,김정민,김소영,이수정,오은실,양서인,김화실,김남초 병원간호사회 2006 임상간호연구 Vol.11 No.2

Purpose: The present study purposed to compare the hand washing effect of 7.5% powidone-iodine, which is used in the operation room of C university hospital in Seoul, with that of Ethyl alcohol-CHG mixture recommended by the US Association of Operating Room Nurses (2002) and to adopt a more effective hand disinfectant. Method: In a quasi-experimental design, 48 medical staff who participated in operations during the period from November 2004 to February 2005 had hand washing using the two kinds of hand disinfectants: 7.5% povidone-iodine and the ethyl alcohol-CHG mixture. Their palms were swabbed and cultured just after hand washing and again after taking dff sterile gloves after the operation. The number of colonies from the two occasions were counted and compared. Result: The number of general bacillus increased significantly in the group using 7.5% povidone-iodine compared to that in the group using the ethyl alcohol-CHG mixture. The number of general bacillus increased signficantly in doctors compared to that in nurses. The factors affecting the increase of the number of general bacillus were disinfectants and medical personnel. The number of general bacillus was expected to increase 9.41 times with 7.5% povidone-iodine than with the ethyl alcohol-CHG mixture and 14.87 times in doctors than in nurses. Conclusion: This study shows that the ethyl alcogol-CHG mixture has a stronger hand disinfection effect than 7.5% povidone iodine. Thus we need to change the hand disinfectant used in operating rooms as soon as possible in order to minimize the infection of wounds resulting from operations.

유아의 또래간 인기도와 대인문제해결력 및 친사회적 행동에 관한 연구

김윤아,전예화 인제대학교 아동ㆍ가족복지연구소 1998 아동ㆍ가족복지연구 Vol.2 No.-

The purpose of this study is to investigate among children, divided into two groups - popular and unpopular - after a popularity test done by themselves, if there are any differences between these two groups in the (inter)personal problem-solving ability and in the prosocial behavior which is evaluated by teachers after individual questionnaires. We will also try to find out if there are any relations between personal problem-solving ability and prosocial behavior. The conclusion of this study is as follows. First, there is among children's peers meaningful difference statistically between popularity and personal problem-solving ability. Second, there is meaningful difference statistically between popularity and prosocial behavior among children's peers. Third, there is no meaningful relation between personal problem-solving ability and prosocial behavior.

부산연안역에서의 대기오염기상 예보시스템 개발에 관한 연구 : 고농도 오존일의 예측을 중심으로 Centering around Forecast of Ozone Episode Day

김유근,이화운,전병일,홍정혜,문윤섭 한국환경과학회 1996 한국환경과학회지 Vol.5 No.4

Pusan is the largest coastal city with a population of about four million in Korea. Because of increased and confused traffic, photochemical air pollution become a major urban environmental problem recently. The photo-chemical air pollution weather forecasting method preciser than existing air pollution forecast method has been developed to forecast ozone episode days with meteorological conditions using the data measured at 7 air quality continuous monitoring stations from June to September using 2 years (1994, 1995). The method developed in present study showed higher percentage correct and skill score than existing air pollution forecasting in KMA (Korea Meteorological Administration).

B형 간염바이러스의 태아와 성인간세포에 대한 감염성 해명 연구

정태호,김성국,서화정,윤영국,임종완,차상호,Chung, Tae-Ho 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2

목적 : 사람의 태아와 성인의 간세포에 B형 간염바이러스의 감염도를 비교 분석함으로써 HBV와 간세포간의 친화도내지는 HBV 수용체에 관한 정보를 얻고자 함. 대상 및 방법 : 성인이 외상을 입어 부득이 손상된 간조직을 절제하였을 경우와 모자보건법상 임신지속이 어려운 상태에서 부인과적 수술시 21주 전후의 태아 간조직을 얻어 DMEM 을 배지로 50% 용량으로 HBsAg 양성인 사람 혈청을 가하여 5일간 체외 배양하고 병리조직학적 처리후 효소면역학적 방법으로 HBsAg 를 염색하여 바이러스의 존재 여부를 검색하였다. 결과 : 태아와 성인 간조직을 5일간 체외 배양하였을 때 그 조직상은 간조직임을 인지할 수 있었다. HBV 의 감염여부는 DAKO 사의 효소면역법으로 조사한 결과 태아의 간조직에서는 HBsAg양성부위를 발견하지 못하였으나, 성인 간조직에서는 HBsAg 양성부위가 발견되었다. 결론 : 태생 21주 전후의 태아의 간세포는 HBV 와 친화도는 아주 미약하거나 없고, 성인 간세포는 HBV에 대한 친화도가 높다. 따라서 태생 21주의 간세포에는 HBV 의 감염이 어렵다는 것을 인지하게 되었다. Considerable progress has been made in the immune prophylaxis of hepatitis B virus(HBV) infection and in the understanding of its replication and pathogenecity at the molecular level. However, a convenient in vitro assay for HBV infectivity has been lacking and the early steps of viral life cycle are now yet well understood. Primary cultured human hepatocytes are reported to be susceptible to HBV 10-12, but these systems are not practical because they are short-lived and require primary explanted human liver. However, it is uncertain yet whether primary hepatocytes of human fetal liver are susceptible to HBV for infection or not. We have expanded the infectivity of HBV into a primary cultures of human fetal hepatocytes together with controversy of adult primary hepatocytes. To investigate the infectivity of HBV for adult and fetal hepatocytes After 5 day culturing fetal(1) and adult(2) liver tissues, which obtained from a surgical operation on DMEM culture media with half HBV positive serum, separating liver cells and fixing them, the existence of HBsAg antigen was investigated through the immuno-enzymatic staining. HBsAg stained area was not found in the 21 weeks gestation liver cells until 5 days of invitro-culture, while it was found in the adult liver cells. This test result showed that the HBV infection is very hard to happen in the liver cells 21 weeks gestation or prior one.

단삼추출물의 항산화능 검색 및 약과에의 첨가효과

김윤화,한영실,백재은,송태희 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.4

This study was carried out to investigate the antioxidant activities of Dansam (S. miltiorrhiza). The Dansam (S. miltiorrhiza) was extracted with ethanol and methanol, and the extracts were fractionated with hexane, chloroform, ethyl acetate and butanol and water, in that order. The antioxidant activities of Dansam (S. miltiorrhiza) were determined by measuring the radical scavenging effects, using the 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) method. The electron donating ability was shown to be about 50% (IC50) at concentration of L-ascorbic acid, Dansam that reflected eliminatory effect by 50% were 9.48㎍/㎖, 8.28㎍/㎖ and 12.59㎍/㎖ respectively. According to the results of the above antioxidation experiments, those for the group with the added Dansam showed a decreased oxidation, but the antioxidation increased with time. With a storage temperature of 60℃ for 5 days, the acid value for the relative antioxidant activities were higher than in the Control group. The peroxide values for the relative antioxidant activities were also higher than in the Control group. The TBA values for the relative antioxidant activities were higher than in the Control group.

율촌 LNG 발전소의 공냉식 복수기 사용에 따른 열확산 수치모의

김유근,이화운,문윤섭,황미경 부산대학교 환경문제연구소 2000 環境硏究報 Vol.18 No.-

In this study, thermal diffusion caused by using air cooled condenser at LNG power plant in Yulchon was simulated through in numerical method (Matsuno scheme). The input data of model was used to seasonal wind components (u, v) of automatic weather system (AWS) and seasonal temperature ranges between inflow and outflow of opening air. The aim of this study was twofold : (1) to investigate sensibility analysis of thermal diffusion coefficient : (2) to simulate diffusion of heat source through the calculated effect height for season and stability variations. These sensibility analyses were performed in detail using two case studies one is to apply horizontal and vertical thermal diffusion coefficient as 50 m-ls-1 and 10 m-ls-1, the other is 30 m-ls-1 and 10 m-ls-1 respectively. It is well known that the sensibility for thermal diffusion coefficient is low owning to very similar results. Consequently, thermal diffusion for the first case is simulated by using effect height for season and stability variation. The temperature is under 1℃ at surface whereas that is high at near the center of effect height.

거대 점오염원이 주변 대기질에 미치는 영향에 관한 연구

김유근,이화운,전병일,장은숙,홍정혜,문윤섭,원경미,송정희 부산대학교 환경문제연구소 1996 環境硏究報 Vol.14 No.1

In order to show the effect of a vast point pollutant source on air quality of Pusan Thermoeletric Power Plant and its surrounding area, air quality around Pusan Thermoeletric Power Plant was simulated by ISCLT-2 which was supplied by EPA. For this purpose the emission amount of SO_2, NO_2 and TSP was calculated and atmospheric stability was classified for a recent decade(1985~1994) in Pusan. A result of the emission amount showed that much amount of NO_2, NO_2 and TSP are emitted from industrial area. It was clear that NO_2 is much emitted from line source and industrial area. And as a result of classification of atmospheric stability, neutral, stable and unstable state were 58%, 24.1% and 17.9%, respectivly. The result of ai quality simulation by ISCLT-2 showed that Pusan Thermoeletric Power Plant is affecting on the increse of 2.0ppb, 3.0ppb and 5.0㎍/㎥, SO_2, NO_2, and TSP respectively at its surrounding area, site A-3 which was located westward 2.2㎞ distance from Plant

마산연안지역의 국지풍 순환 및 대기오염물질 확산에 관한 연구

김유근,이화운,문윤섭,김해동,원경미,정우식,오인보 부산대학교 환경문제연구소 1997 環境硏究報 Vol.15 No.1

To investigate a horizontal windrose and the mixing height in Masan coastal area, a local atmospheric flow and the concentration of air pollutants were analyzed by abserved data from AWS, airsonde and air sampler. The land-sea breeze model and the ISCST2 model of U. S. EPA(Environmental Protection Agency) were used to predict the concentration of air pollutants such as SO2 and TSP. As a result, during spring the principal wind direction in the Masan region is mainly represented by windrose of the WNW and NW, which can be induced by land breeze and the SE and SSW of sea breeze even if weak wind speed. The mixing height during the measurement was marked with the range from 400 m(0900 LST) to 1450 m(1500 LST), and the height of an inverse layer was presented as the range from 30 m(2100 LST) to 150 m(0300 LST). It is very important to cosider the local wind field for the accurate diffusion and movement of air pollutants in coastal urban area. The atmospheric flow was simulated by the two-dimensional local wind model upon considering the orographic characteristics such as land-sea contrast, mountain valley and urban building. The result of air quality simulated by ISCST2 support that the dispersion pattern of the pollutants in that area was followed as local circulation system in coastal urban area.

Magnetic bead capture 를 이용한 염색체 띠 7q11, 7q22 에 위치한 cDNA 선별

김영수,김의숙,강윤희,전용혁,김현,박선화 고려대학교 의과대학 1997 고려대 의대 잡지 Vol.34 No.3

Recently, surmountable amounts of genes are being cloned and it has become neccessary to develope new techniques for discovering genes with known chromosomal location and their possible functions. We have developed one such a method and applied it to search for genes that may play a functional role during the development of the central nervous system. To observe the distribution of 18 weeks old fetal brain cDNAs on the chromosome, fluorescent in situ hybridization (FISH) was performed with biotin labeled 18 weeks old fetal brain cDNAs. Among the chromosome bands showing strong hybridization with the cDNAs, 7q11 and 7q22 were microdissected and amplified by PCR. Each band-specific probe pool was confirmed by FISH and band specific cDNAs were selected by magnetic bead capture method. Selected cDNAs were subcloned to plasmid vectors and the nucleic acid sequences were analysed. As a result 41 different clones from 7q11 and 43 different clones from 7q22 were obtained. They were categorized as 25 clones of well characterized genes, 22 clones showing low homology with known genes, 13 clones of simply registered uncharacterized human cDNAs, and 24 clones of unknown genes. From these results, it may be suggested that our technique is very useful to clone the genes expressed in the developing human brain with confirmed chromosomal location. In addition, this cloning technique can be used to discover the new genes related with neural development in combination with functional screening methods.

Sulfadimethoxine 투여에 의해 유발된 갑상선 증식성 병변에 나타난 피막 섬유화의 병리학적 특성

김혜성,손화영,조성환,최종윤,윤원기 충남대학교 수의과대학 동물의과학연구소 2001 動物醫科學硏究誌 Vol.9 No.-

Sulfadimethoxine (SM) is one of the anti-thyroid (goitrogenic) agents and has a thyroid tumor promoting effects. In this study, we analyzed the pathology of SM induced thyroid proliferative lesions to see if these lesions showed any distinctive features. Five-week-old male F344 rats were given a single subcutaneous injection of N-bis(2-hydroxypropyl)nitrosamine (DHPN: 2800㎎/㎏, body weight). Starting one week thereafter, SM (1000 ppm in drinking water) was administered for 12 weeks. All animals were autopsied and histopathological observation of the thyroids was perfomed. Thyroid follicular proliferative lesions were induced in all rats treated with SM. In addition, the formation of thick perithyroidal fibrous capsule and pleomorphic follicles within the capsule were observed. These pleomorphic follicles showed invasive figure in some area, especially around follicular adenomas and adenocarcinomas. This confuses adenoma with adenocarcinoma. However, pleomorphic follicles were also observed in the area without adenomas and adenocarcinomas. Therefore, we suggest that it should be careful to diagnose adenoma and adenocarcinoma in the presence of the invasive lesions in surrounding fibrous tissue, especially SM induced thyroid lesion.