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RISS 인기검색어
- 검색키워드
- 저자 : Yuheon Chung (검색결과 5 건)
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Upregulation of autophagy by Ginsenoside Rg2 in MCF-7 cells
Chung, Yuheon,Jeong, Seula,Choi, Hyun Seok,Ro, Seungil,Lee, Jung Sup,Park, Jong Kun ZOOLOGICAL SOCIETY OF KOREA 2018 ANIMAL CELLS AND SYSTEMS Vol.22 No.6
<P><B>ABSTRACT</B></P><P>Autophagy is a major intracellular degradation process that plays an important role in cell survival, stress responses, nutrient sensing and development. Our previous studies have shown that Rg2, a triterpenoid saponin contained in ginseng, protects cells against UVB-induced genotoxicity by increasing DNA repair, in possible association with modulation of protein levels involved in p53 pathway. In this study, we determined an upregulation of autophagy by Rg2. Rg2 treatment for 24 h in MCF-7, a breast cancer cell, did not show cytotoxicity up to 200 μM. Rg2 also upregulated the level of p-p53, p-AMPK, p-ACC, Atg-7 and LC3-II and decreased the level of p62 in concentration-dependent manners. We also determined the level of p53, AMPK, p62, Atg-7 and LC3 after UVB exposure and subsequent incubation in growth medium for 24 h. UVB increased the level of p-p53, p-AMPK, p-ACC and decreased the levels of p62, Atg-7 and LC3-II. Interestingly, Rg2 treatment for 24 h after UVB exposure increased the levels of p-p53, p-AMPK, p-ACC, Atg-7 and LC3-II and decreased the level of cyclobutane pyrimidine dimer, a UVB-induced DNA damage in concentration-dependent manners. All these results suggest that Rg2 increased autophagy and decreased UVB-induced DNA damage, in possible association with the modulation of protein levels in p53- and autophagic pathways.</P>
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정슬아,Chung Yuheon,Moon Jonghyun,Kim Youngun,Kim Seulhye,Baek Gain,Park Jong Kun 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.4
Background Lead (Pb), a heavy metal, is contained in large amounts of particulate matter (PM). The greater production of PM through agricultural operations, industrial processes, and combustion of fossil fuels concomitantly increases human exposure to lead through the respiratory system. Lead is highly toxic even when exposed in small amounts to the body and induces respiratory diseases, increased production of reactive oxygen species (ROS), and DNA damage. Dioscorea polystachya (DP) possesses antioxidant and anti-inflammatory effects and is used as a traditional herbal remedy to treat respiratory diseases. However, the effects of DP on oxidative DNA damage remain unclear. Objective In this study, we determined the protective effects of DP ethanol extracts (DEE) against the lead acetate-induced oxidative DNA damage in MRC-5 cells—a human lung fibroblast cell line. Result MRC-5 cells were co-treated with 200 μM of lead acetate and various concentrations of DEE for 48 h. Cell viability, which decreased by approximately 40% after treatment with lead acetate alone, showed an approximately 34% increase upon treatment with 500 μg/mL DEE. The increased levels of 8-OHdG, DNA fragmentation and cellular ROS observed upon treatment with lead acetate, decreased in a DEE concentration-dependent manner. Furthermore, the levels of DNA damage response (DDR)-related proteins, such as p53, p-p53, and p21 increased, and the levels of apoptosis-related proteins, such as Bax and Bim decreased in a DEE concentration-dependent manner. Conclusion Our results demonstrate the protective effects of DEE on lead acetate-induced oxidative DNA damage, mediated through a reduction in the levels of cellular ROS and an increase in those of DDR-related proteins at early time points.
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Jeong Seula,Chung Yuheon,박소진,Lee Sumin,Choi Nayoung,Park Jong Kun 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.1
Background Ultraviolet B (UVB), a major cause of skin photoaging, leads to DNA damage directly and indirectly in skin cells. DNA damage can contribute to aging by increasing apoptosis, cellular senescence and cell dysfunction. To prevent photoaging and rejuvenate photoaged skin, combinations of active compounds from natural products are used for medicine and cosmetic ingredients. Objective In this study, we investigated the synergistic effects of Rg2 and piceatannol (PIC) mixture (RP) on protecting skin against UVB-induced DNA damage in HaCaT cells. Results Cells treated with RP for 24 h post UVB exposure increased the cell viability in a concentration-dependent manner compared to non-treated control. In DPPH and DCF-DA assay, RP showed similar free radical scavenging activity to that of single-treatment PIC. RP more decreased the levels of CPD, DDR proteins including p53, p-p53 and p21 and apoptosisrelated proteins, such as Bax, Bim, and cleaved caspase-7 than that of single-treatment PIC or Rg2. Also, RP decreased more the mRNA level of MMP-1, -3 and -9 than that of single-treatment PIC or Rg2. Conclusion It is inferred that Rg2 promotes DNA repair by regulating genes related with DDR and PIC promotes DNA repair by effectively scavenging UVB-induced ROS through high antioxidant activity. Enhanced DNA repair by RP leads to protecting skin from UVB-induced skin photoaging.
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자외선 B에 의해 유도되는 DNA 상해에 대한 참갈파래 메탄올 추출물의 보호 효과
정슬아,정유헌,박종군,Jeong, Seula,Chung, Yuheon,Park, Jong Kun 한국식품영양학회 2020 韓國食品營養學會誌 Vol.33 No.3
In this study, we investigated the protective effects of Ulva lactuca methanol extracts against ultraviolet B (UVB)-induced DNA damage in HaCaT cells. First, the contents of general and antioxidative nutrient contents of Ulva lactuca were measured. The moisture, carbohydrate, crude protein, crude fat and ash were 14.01%, 44.80%, 23.19%, 3.10% and 14.90%, respectively. Magnesium that acts as DNA repair enzyme cofactor was the most abundant mineral followed by Ca, P and Fe. The total phenolic and anthocyanoside contents of Ulva lactuca were 2.69 mg/g and 0.13 mg/g, respectively. Cells treated with Ulva lactuca methanol extracts for 24 hours post UVB exposure increased cell viability in a concentration-dependent manner compared to the non-treated control. Also, Ulva lactuca methanol extracts decreased the levels of UVB-induced DNA damage such as cyclobutane pyrimidine dimer and DNA damage response (DDR) proteins such as p-p53 and p21. These results suggest that Ulva lactuca methanol extracts comprising physiological active substances such as Mg, polyphenols and anthocyanosides promote DNA repair by regulating genes related with DDR.
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