PCR 기법을 이용한 한국재래산양 β-casein 유전자의 특성

김지애,류승희,유성란,이준헌,서길웅,김선균,상병찬 충남대학교 농업과학연구소 2002 농업과학연구 Vol.29 No.2

본 연구는 한국 재래 산양 112두와 유산양인 Saanen종 7두의 혈액으로부터 genomic DNA를 추출하고, PCR-RFLP 방법에 의해 β-casein 유전자의 특성을 분석하여 한국재래산양의 효율적인 유전자원의 보전 및 개량을 위한 기초 자료로 제공하고자 실시하였다. 한국재래산양의 genomic DNA로부터 PCR기법을 이용하여 β-casein의 유전자좌를 증폭한 결과 각각 481bp 크기의 단편이 양호하게 증폭되었음을 확인하였다. β-casein 유전자좌의 증폭산물에 대한 Bal Ⅰ의 제한효소를 처리한 결과, β-casein AB형은 481bp, 284bp 및 197bp의 단편을, 그리고 BB형은 284BP와 197BP의 단편을 한국재래산양과 유산양인 Saanen 종에서 확인 할 수 있었다, 유전자형 빈도에 있어서는 한국재래산양에서 β-casein AB 및 BB의 빈도는 각각 6.25 및 93.75%이었고, 유산양인 Saanen 종은 각각 57.14 및 42.86%이었다. 유전자빈도에 있어서는 한국재래산양의 β-casein A 및 B의 빈도가 각각 0.031 및 0.969이었고, Saanen 종에서는 각각 0.286 및 0.714의 빈도를 보였다 한국재래산양의 β-casein 유전자의 염기서열과 이미 보고되어 있는 goat의 염기서열(GeneBank accession Number M90556)간에는 총 11개의 염기서열에 차이를 나타내어 97.71%의 상동성을 보였다, 따라서 한국재래산양의 β-casein 유전자의 다형성과 염기서열 분석에 의한 분자유전학적 특성의 규명은 한국재래산양의 유전자원의 보전 및 개량을 위한 기초 및 응용 자료로 이용될 수 있을 것으로 생각된다. This study was performed to provide the basic data for preservation and improvement of genetic resources according to finding genetic construction obtained from analysis of genetic characteristics of β-casein gene in Korean Native goat and Saanen using the PCR-RFLP. This study confirmed the amplified products of 481bp fragments obtained from the amplification of β-casein loci by PCR. The β-casein AB genotype showed 481, 284 and 197bp, and β-casein BB genotype showed 284 and 197bp fragments in Korean Native goat and Saanen. The frequencies of β-casein genotype in Korean Native goat were 6.25 and 93.75% for AA and AB and the frequencies of β-casein genotype in Saanen were 57.14 and 42.86% for AA and AB types. The frequencies of β-casein A and B alleles were 0.031 and 0.969 in Korean Native goat and the frequencies of β-casein A and B alleles are 0.286 and 0.714 in Saanen, respectively. The nucleotide sequence of β-casein gene of Korean Native goat was 97.71% higher homology with 11 nucleotide sequences difference of that of goat reported in GeneBank (M90556). Therefore, this study of molecular genetic characteristics by the analysis of genetic polymorphism and sequencing for β-casein gene should be used as basic and applying data for preservation and improvement of genetic resources in Korean Native goat breeding.

지역학으로서의 독일학 육성을 위한 정보자료의 디지털화와 활용방안에 관한 연구

최병진,정동규,천미애,남유선 한국독어독문학회 2003 獨逸文學 Vol.88 No.-

In dieser Arbeit wurde vor allem versucht, den Blick auf zwei Punkte zu richten: Erstens sollte das Forschungsvorhaben, das im Rahmen eines zweija¨hrigen Projekts unter finanzieller Unterstu¨tzung des Koreanischen Research Foundation (KRF) durchgefu¨hrt wurde, in grobem Abriss vorgestellt werden. Zweitens werden die Probleme skizziert, die wa¨hrend der Durchfu¨hrung des Projekts auftauchten, und aus denen sich schließlich unmittelbar einige Verbesserungs- vorschla¨ge ergeben. Das Projekt hatte die Aufgabe, ein auf digitalisierten Daten basierendes Informationssystem u¨ber Deutschland zu erstellen. Das System der Wissenschaftsinformationen, das in der vorliegenden Arbeit vorgestellt wurde, wird beispielsweise aufgrund seiner umfangreichen Informationsdaten dem Fach Germanistik zur weiteren und vertiefenden deutschlandeskundlichen Forschung von großem Nutzen sein. Daru¨ber hinaus aber kommt es auch den Lehrenden zugute, da ihnen dann ein vielseitiges und reichhaltiges Lehrveranstaltungsmaterial als Veranstaltungsthemen zur Verfu¨gung steht. Um ein solches Wissenschaftssystem der Deutschlandforschung zu erstellen und weiter zu entwickeln, wird jedoch nicht nur vie1 Zeit, sondern eine sta¨ndige Aktualisierung der Datenbank no¨tig werden. Um diese Aufgaben zu bewa¨ltigen, sollten mo¨glichst viele Germanisten fu¨r das Projekt zur Verfu¨gung stehen. Zurzeit findet das Internet im privaten und o¨ffentlichen Bereich breite Anwendung wie noch nie zu vor, und diese Tendenz wird sich noch versta¨rken. Forscher suchen und sammeln Informationen anhand des Internets. So nimmt es im Forschungsleben als Forschungswerkzeug einen wichtigen Platz ein. In Zukunft wird sich der Service im Bereich des Netzwerks weiter entwickeln/verbessern. Dies bedeutet fu¨r die Forscher eine Chance im Hinblick auf die Zukunft der Forschung, wenn sie gemeinsam ein wissenschaftliches Kooperationssystem entwickeln, um Wissen unter einander auszutauschen.

시각장애 동화구연자의 학습 과정에 나타난 특징과 의미 연구

유애순 ( Ae-sun Yu ) 방정환연구소 2019 방정환연구 Vol.1 No.1

이 연구는 시각장애 동화구연자의 학습 과정에 나타난 특징과 의미 연구를 목적으로 한다. 동화구연은 구연자와 청중의 상호 공감 속에서 언어와 비언어적표현이 조화를 이루는 예술 활동이다. 특히 구연자와 청중 사이의 시선교감은 동화구연 과정에서 매우 중요한 요소이다. 이 논문은 시각장애 구연자가 제한적 조건에서 청자와 어떻게 상호교감을 할 수 있는지 밝히고 그 의미를 제시하였다. 시각장애 동화구연자의 특수한 상황을 고려할 때 이에 적합한 학습 활동은 활동보조인의 도움으로 해소될 수 있다. 이 논문에서는 시각장애 동화구연자의 학습이 어떻게 이루어지는가에 대한 전반적인 과정과 특징을 단계적으로 다루었다. 그 과정은 실제 학습과정의 예와 학습자의 인터뷰내용으로 이루어졌다. 시각장애인 학습자는 학습 과정을 통해 이야기 도입, 마음자세, 발음과 인물 분석 구연 연습, 신체 언어 학습의 비언어적 활동을 이해하고 배울 수 있다. 시각장애 동화구연자는 이러한 학습 과정을 거치면서 전문가 수준으로 성장해 나갔으며, 그 자신의 삶의 주체가 되어 청중에게 감동을 주는 예술행위를 전달하게 되었다. This study aims at seeking the features and the significances in the blind storyteller’s learning process. Storytelling is considered to be an art activity whereby language and non-verbal expressions are harmonized in the mutual sympathy between the audience and the storyteller. Sharing the feeling through the eye-contact between the storyteller and the audience, in particular, is one of the critical factors during the story-telling process. Hence, this study attempts to identify how the blind storytellers communicate with the audience and deliver the message to them under their constraints, suggesting its significances. For the special conditions those blind storytellers have, the study suggests that appropriate learning activities should be provided with the help of an assistant so that the restrictions are eliminated during the storytelling process. The study also presents the overall process and the features of how such blind storytellers carry out their performance, for which it suggests a step by step method. This process is conducted by the interview with the learners who have actually attended the storytelling class. Those blind learners could understand and learn the introduction of a story, mind posture, pronunciation and character analysis and also non-verbal activities of body language. Through this learning process, those blind storytellers eventually get to be the level of a professional storyteller, by which they have become a subject of life who enjoys delivering an art act impressing their audience.

소양증(瘙痒症)의 침구치료(鍼灸治療)에 관한 고찰(考察)

유선애 ( Sun Ae Yu ),김철홍 ( Cheol Hong Kim ),윤현민 ( Hyoun Min Youn ),이승연 ( Seung Yeun Lee ) 대한경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.3

Objective: The purpose of this study is to review the acupuncture therapy and moxibustion on itching Method: We searched and reviewed chinese journals of and books of the acupuncture therapy and moxibustion on itching. Results & Conclusions: 1. Dermatopathy of itching is described as eczema, neurotic-dermititis, itching in oriental medical text. 2. According to text, itching causes were wind symptom produced by deficiency of blood, deficiency syndrom of the spleen, and damp-heat. 3. Frequently used acupoints were LI10(41 frequency), SP10(29), ST36(27), SP6(22), GB20(19), Asi point(18), BL(17), back Su acupoints(13), LI4(13), SP9(10), PC6(7), HT7(7). etc. 4. The practice of acupuncture was used by body acupuncture, auriculo-theraphy, loss of bleeding with needle, blum-blosson needle, dermal needle, fire needle. etc. 5. Moxibustions are mild moxibustion, non-scar producing moxibustion, herb- moxibustion. etc.

Drosophila short neuropeptide F signalling regulates growth by ERK-mediated insulin signalling

Lee, Kyu-Sun,Kwon, O-Yu,Lee, Joon H.,Kwon, Kisang,Min, Kyung-Jin,Jung, Sun-Ah,Kim, Ae-Kyeong,You, Kwan-Hee,Tatar, Marc,Yu, Kweon Nature Publishing Group 2008 Nature cell biology Vol.10 No.4

Insulin and insulin growth factor have central roles in growth, metabolism and ageing of animals, including Drosophila melanogaster. In Drosophila, insulin-like peptides (Dilps) are produced by specialized neurons in the brain. Here we show that Drosophila short neuropeptide F (sNPF), an orthologue of mammalian neuropeptide Y (NPY), and sNPF receptor sNPFR1 regulate expression of Dilps. Body size was increased by overexpression of sNPF or sNPFR1. The fat body of sNPF mutant Drosophila had downregulated Akt, nuclear localized FOXO, upregulated translational inhibitor 4E-BP and reduced cell size. Circulating levels of glucose were elevated and lifespan was also extended in sNPF mutants. We show that these effects are mediated through activation of extracellular signal-related kinases (ERK) in insulin-producing cells of larvae and adults. Insulin expression was also increased in an ERK-dependent manner in cultured Drosophila central nervous system (CNS) cells and in rat pancreatic cells treated with sNPF or NPY peptide, respectively. Drosophila sNPF and the evolutionarily conserved mammalian NPY seem to regulate ERK-mediated insulin expression and thus to systemically modulate growth, metabolism and lifespan.

Poster Session 1 : Metabolism and Enzymology Protein Structure and Function ; Purification and biochemical characterization of novel protease from the Korean polychaeta, Periserrula Leucophryna

( Yu Rim Seo ),( Sun Ae Choi ),( Ji Hoon Lim ),( Han Seung Joo ),( Jang Won Choi ),( Chung Soon Chang ) 한국생화학분자생물학회 (구 한국생화학회) 2004 생화학분자생물학회 춘계학술발표논문집 Vol.2004 No.-

Improving the Productivity of Single-Chain Fv Antibody Against c-Met by Rearranging the Order of its Variable Domains

( Yu Jin Kim ),( Rameshkumar Neelamegam ),( Mi Ae Heo ),( Selvakumar Edwardraja ),( Hyun Jong Paik ),( Sun Gu Lee ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.6

Life Science : A comparative proteomic analysis for capsaicin-induced apoptosis between human hepatocarcinoma (HepG2) and human neuroblastoma (SK-N-SH) cells

( Yu Mi Baek ),( Hye Jin Hwang ),( Sang Woo Kim ),( Hee Sun Hwang ),( Sung Hak Lee ),( Jung Ae Kim ),( Jong Won Yun ) 영남대학교 약품개발연구소 2009 영남대학교 약품개발연구소 연구업적집 Vol.19 No.-

Multiple metastasis of follicular variant of papillary thyroid carcinoma coexistent with malignant melanoma

( Yu Ji Kim ),( Kyung Ae Lee ),( Tae Sun Park ),( Hong Sun Baek ),( Heung Yong Jin ) 대한내과학회 2018 The Korean Journal of Internal Medicine Vol.33 No.3

임상실습에 대한 간호사와 간호학생의 교수효율성 인지정도

유필숙,김은실,김혜경,이성희,형희경,엄애용,김희선 대한간호행정학회 2008 간호행정학회지 Vol.14 No.3

Purpose: This study was conducted to use as a preliminary data to improve the quality of clinical nursing education by comparing the perception of teaching effectiveness in nurses and nursing students. Method: The participants were 165 nurses and 289 nursing students. Data was collected from June 2 to 16, 2008. The research instruments used an effective clinical instructors which was developed by Reeve(1994). Result: The perception of teaching effectiveness in nurses and nursing students were 3.45 and 3.19 respectively and it showed that nurses' perception of teaching effectiveness are higher than that of nursing students. Nurses had strong points in the factors of 「interpersonal relationship/ communication」, 「availability as a supporter」, 「teaching method and evaluation」. In additions, the teaching effectiveness had significant differences to age, years of work, position, educational background and job satisfaction in nurses, otherwise, sex, differences of educational level, satisfaction of clinical practicum and major in students. Conclusion: It is needed a further study as below: to develop an education system which is to improve an interpersonal relationship and to upgrade the quality of educator; to develop a teaching method program of clinical nursing education to improve teaching effectiveness.