들깨(Perilla frutescens) 새싹 추출물의 항산화 및 항염 효과

정승일(Seung-Il Jeong),김현수(Hyeon Soo Kim),전인화(In Hwa Jeon),강현주(Hyun Ju Kang),목지예(Ji Ye Mok),천춘진(Chun Jin Cheon),유현희(Hyeon Hee Yu),정선일(Seon Il Jang) 한국식품과학회 2014 한국식품과학회지 Vol.46 No.1

본 연구는 들깨 새싹 추출물의 항산화, 항염증 및 항부종에 대한 효과를 조사하였다. 들깨 새싹 추출물은 DPPH와 ABTS 라디칼을 효과적으로 제거하는 항산화 활성이 우수하였다. 또한 들깨 새싹 추출물은 활성화된 설치류 유래 대식세포주인 RAW 264.7세포와 인간 유래 HMC-1 세포의 TNF-α와 IL-1β를 효과적으로 억제하였다. 더욱이 마우스의 귀와 발 부종을 억제하는 우수한 효과가 있었다. 이러한 결과는 들깨 새싹 추출물은 항산화제로 사용될 수 있을 뿐만 아니라 항염증과 항부종에 효과적인 물질이라는 것을 제시해주었다. 이와 관련된 들깨 새싹 추출물의 기능성에 대해서는 앞으로 분자생화학적 수준에서 더 연구해야할 필요성이 있는 것으로 사료된다. We investigated the effects of an ethanol extract from Perilla frutescens sprouts (PFSE) as an antioxidant, and its effects on edema and inflammation in RAW 264.7 cells and HMC-1 cells. The antioxidant activities (DPPH and ABTS radical scavenging) of PFSE were similar to those of butylated hydroxytoluene (BHT) and (±)-6-hydroxy-2,5,7,8- tetramethylchromane-2-carboxylic acid (Trolox). We also investigated the anti-inflammatory effects of PFSE on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and HMC-1 cells stimulated with phorbol 12-myristate 13-acetate (PMA) with the calcium ionophore A23187. TNF-α and IL-1β production, which had been increased by treatment with LPS or PMA plus A23187, were significantly inhibited by PFSE in a dose-dependent manner. Furthermore, PFSE significantly reduced the xylene-induced ear edema and the carrageenan-induced paw edema of ICR mice in a dosedependent manner. The effects of PFSE (200 mg/kg) in reducing ear and paw edema were similar to those of aspirin (50mg/kg). These results suggest that PFSE can be potentially used as a medicine for treating oxidative stress, an edematous and inflammatory disease.

심한 저칼륨혈증을 동반한 갑상선 중독성 주기성 마비 2례

유승진,최승필,최경호,오동렬,황주일,나병호,김세경 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

Thyrotoxic periodic paralysis occurs primarily in Orientals with an overwhelming male preponderance and a higher association of specific HLA antigens. Clinical manifestations include onset after high carbohydrate ingestion or heavy exertion with progressive symmetric weakness leading to flaccid paralysis of the extremities and other muscle groups, lasting several hours. Other precipitating facters include trauma, infection, administration of epinephrine, thyroid hormone, or corticosteroid. If hypokalemia is present, potassium administration may help abort the attack. Although propranolol can be efficacious in preventing further episodes, the only definitive treatment is euthyroid state. The pathophysiology is still controversal, but reflects altered potassium and calcium dynamics as well as certain morphologic characteristics within the muscle unit itself. We discribed two patients with thyrotoxic periodic paralysis who had a no familial history of paralysis and had a decreased level of serum potassium during attack.

자일리톨과 불화나트륨을 함유한 저작성 정제가 교정환자의 구강위생에 미치는 영향에 관한 임상시험

유상훈,안석준,이신재,백승학,김태우,장영일,남동석 대한치과교정학회 2003 대한치과교정학회지 Vol.33 No.2

본 연구는 불화나트륨과 자일리톨 성분으로 구성된 구강 청정용 저작성 정제(덴포정, 하미즐, 부천, 경기)가 교정치료 중인 환자의 구강위생 상태에 미치는 영향을 알아보기 위해 시행되었다. 교정치료 중인 18세 이상의 성인 환자 30명을 대상으로 약제 상요 전, 2주 후, 4주 후에 피험자의 상악과 하악 전치 부위 및 구치 부위에서 각각 치은 지수, 치은 출혈 지수, 치태 지수를 측정하여 다음과 같은 결과를 얻었다. 1. 상하악 전치부와 구치부 모두에서 치태 지수가 약제 사용 전에 비해 사용 2주 후와 4주 후 유의성 있게 감소하였다(p<0.05). 또한, 적용 2주 후와 4주 후의 지수를 비교하였을 때 4주 후에 좀 더 개선된 경향을 보였으나, 통계적 유의성은 나타나지 않았다(p<0.05). 2. 치은 지수, 치은 출혈 지수도 상하악 전치부와 구치부 모두에서 적용 전에 비하여 2주 후와 4주 후 유의성 있게 감소하였다(p<0.05). 적용 2주 후와 4주 후의 지수의 비교 검증에서 통계학적으로 유의할 만한 차이가 보이지 않아 구강위생 상태의 개선이 계속 유지됨을 보였다. 본 연구의 결과는 치태 지수, 치은 지수, 치은 출혈 지수가 실험 정제 적용 후에 감소하여 구강위생 상태가 개선되었음을 보였다. 이러한 결과를 통해 덴포정을 교정치료 중인 환자에서 부가적으로 사용하였을 경우 치주조직의 건강을 증진시키는데 도움을 줄 수 있으리라고 생각된다. The aim of this study was to evaluate the effect a chewable tablet containing sodium fluoride and xylitol (DenPo, Hamizzle, Bucheon, Kyungki) on the oral hygiene state in the orthodontic patients. The subjects consisted of 30 adult orthodontic patients, who have worn the fixed orthodontic appliance at least 6 months. They were instructed to use the tablet forth a day. Gingival index (GI), plaque index (PI), and sulcus bleeding index (SBI) of upper and lower anterior and posterior regions were measured before using the tablet (TO), 2 weeks after using the tablet (T1), and 4 weeks after using the tablet (T2). The data were analyzed with Wilcoxon signed ranks tests. The results were as follows ; 1. PI was decreased significantly in the T1 and T2 stage compared with TO stage in the both anterior and posterior regions (p<0.05). Although PI was decreased in the T2 stage compared with T1, there was not statistical significance(p>0.05). 2. GI and SBI showed similar results compared with PL. In the T1 and T2 stage, there was statistically significant decrease in the GI and SBI (p<0.05). This study showed that the DenPo tablet could be used as an adjunct to improve oral hygiene in the orthodontic patients. The decrease of the indices may suggest that DenPo tablet could be effective in the plaque removal and prevention of gingival inflammation in the orthodontic patients.

3-MCPD의 생식˙발생독성에 관한 연구

곽승준(Seung Jun Kwack),김순선(Soon Sun Kim),최요우(Yo Woo Choi),이규식(Gyu Seek Rhee),손경희(Kyung Hee Sohn),이이다(Rhee Da Lee),채수영(Soo Young Chae),정용현(Yong-Hyun Chung1),유일재(Il Je Yu1),박귀례(Kui Lea Park) 한국독성학회 2004 Toxicological Research Vol.20 No.2

3-Monochloro-1,2-propanediol(3-MCPD) is a toxic compound, often present in different foods containing acid hydrolyzed(AH) protein, like seasonings and savory food products. The purpose of the present study was to investigate the effects of 3-MCPD on male fertility, sperm and testosterone secretion. In vivo male fertility test was performed for observing the adverse effects of 3-MCPD on the function of male reproductive system and pregnancy outcome. 0.01, 0.05, 0.25, 1 and 5 mg/kg b.w. of 3-MCPD was given daily by gavage to groups of 15 adult male SD rats for 4 weeks. At the end of pre-treatment period, males were mated overnight with normal females. Following morning, males demonstrating successful induction of pregnancy were sacrificed on that day to assess sperm parameters and histopathology of reproductive organs. The resulting pregnant females were sacrificed on day 20 of gestation to evaluate pregnancy outcome. As a result, four-week paternal administration with 3-MCPD resulted in adverse effects on male fertility and pregnancy outcome without remarkable histopathological changes in testes and epididymides; sperm motility, copulation index and fertility index were markedly decreased in the treated group and numbers of live fetuses showed steep dose-response curves. Also, spermatogenesis was investigated in this experiment. However, no<br/> effect was observed on production of sperm in testes treated with 3-MCPD for 4 weeks. Hormone assay was performed for observing the effects of 3-MCPD on testosterone and luteinizing hormone (LH) in blood and testes of male SD rats and cultured primary Leydig cell. In result, significant changes of related hormones did not observed by treatment of 3-MCPD. These results indicated that paternal treatment with 3-MCPD induced spermatotoxic effect, which caused an antifertility on male.

심한 낙타혹(Hump)의 형성을 동반한 제 2 형 막증식성 사구체신염(Dense Deposit Disease) 1예

오희정,유민아,이영숙,김미연,김승정,강덕희,윤견일,최규복,성순희 이화여자대학교 의과대학 2004 EMJ (Ewha medical journal) Vol.27 No.1

Dense deposit disease (DDD), known as type Ⅱ MPGN, is an uncommon form of glomerulonephritis. It is an acquired primary glomerular disease, characterised by electron microscopic evidence of a continous, dense membrane deposition replacing the lamina densa. There is associated alternative pathway complement activation and a C3 nephritic factor maybe present. Patients with dense deposit disease tend to be young at clincal onset and more commonly have persistent nephrotic syndrome, gross hematuria, and acute nephritis episode and persistent depression of the serum complement level. The prognosis of dense deposit disease is worse than that of typeⅠMPGN. We experienced a patient with dense deposit disease that presenting persistent nephrotic syndrome, gross hematuria and having subepithelial hump. It is a first report of dense deposit disease having subepithelial hump in Korea, therefore we report this case with the review of relevant literatures.

Terfenadine과 Ketoconazole 복용 후 발생된 Torsades de pointes 심실 빈맥증의 치험 1례

김세경,이기중,유승진,박일영,박승현,임근우,이원재,강동헌 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Torsades de pointes is a form of polymorphic ventricular tachycardia that is associated with prolongation of the QT interval, characterized by QRS complexes of progressively changing amplitude and contour that seem to revolve around the isoelectric line. The most common cause of Torsades de pointes induced by antiarrhythmic agents, occupying in certain drugs or toxin, electrolyte disturbance, congenital QT prolongation syndrome. Cardiotoxicity terfenadine, in overdose a widely used, nonsedating antihistamine, has been reported. Case after administration of terfenadine and ketoconazole causing syncope and torsades de pointes have been reported in recommended dose. We report a case of QT prolongation with torsades de pointes after therapeutic administration of terfenadine, a long acting histamine H1 receptor antagonist, together with ketoconazole, an antifungal preparation. The patient is a 43-year-old female, who experienced palpitation, dizziness and syncopal episoders after coadministration of terfenadine and ketoconazole, for a fungal infection of the skin. The ECG showing typical episodes of torsades de pointes with QT prolongation. Magnesium sulfate was given, torsades de pointes did not recur. The patient was discharged with improved condition.

응급실에 내원한 약물중독환자의 지역별 비교분석

임근우,박일영,유승진,나병호,오동렬,전해명,황주일,김세경 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

We reviewed retrospectively 230 patients who came to emergency room of Kang Nam St. Mary's Hospital and Uijongbu St. Mary's Hospital between 1. January and 31. December 1994. We classified them according to their age, sex, a sort of drug, the cause of poisoning, prehospital care, time-lapse to arrive at hospital after poisoning, psychiatric problem the status of patients. *We conclude as follows; 1. The male to female ratio was totally 1 : 1.98, 1 : 1.24 in a urban area, 1 : 2.43 in a rural area. 2. On age of the patients in this study, the peake incidence was between 20 and 39(55.2%). 3. On seasonal prevalence, the poisoning was most common in summer and spring, in urban area(35.7%) and rural area(37.0%) respectively. 4. The common drug were as follow; Doxylamine, Organophosphate, Acetaminophen in urban area, Paraquat, Organophospate, Doxylamine in rural area 5. The percentage of suicidal attempt were 81.8% and 87.7% in urban area and rural area respectively. 6. The poisoning patients had psychatric problem in 19.4% and 24.6%, in urban area and rural area respectively. 7. Before arrival to emergency room, 27.7% of patient was done prehospital care in rural area. 8. On arrival to emergency room, 95.1% and 73.8% of patients show stable vital sign in urban area and rural area respectively.

가역 투자율 측정에 의한 1Cr-1Mo-0.25V 강의 열화도 평가

유권상,김용일,남승훈,유광민,조육,손대락 한국비파괴검사학회 2000 한국비파괴검사학회지 Vol.20 No.5

고온에서 운용 중인 설비의 안전성을 평가하기 위해서는 사용기간동안 열화된 재료의 물성을 측정하여야 한다. 실제 사용되고 있는 화력발전소 터빈의 로터에서 열화도가 다른 여러 종류의 시편을 입수하기 어렵기 때문에, 터빈 로터재로 널리 사용되고 있는 1Cr-1Mo-0.25V 강을 인공열화시켜 시편으로 사용하였다. 열화도의 비파괴적 평가를 위하여 교류 섭동 자기장을 인가하여 가역 투자율을 측정하는 자기적 방법을 사용하였다. 열화도의 증가에 따라 경도와 가역 투자율 피크 사이의 간격은 감소하였는데, 경도와 가역 투자율 피크 간격과의 선형관계를 이용하여 비파괴적으로 터빈 로터강의 열화도를 평가할 수 있는 기초를 마련하였다. The integrity of the turbine totors can be assessed by measuring reversible permeability and Vickers hardness of the aged rotors at service temperature. The measurement system of reversible permeability, which measured by applied alternating perturbing magnetic field, was constructed in order to evaluate matrial degradation, nondestructively. The test specimen was 1Cr-1Mo-0.25V steel used widely for turbine rotor material, and the specimens were prepared by the isothermal heat treatment at 630 ℃. The reversible permeability of the test materials were measured at room temperature. The peak interval of reversible permeability and Vickers hardness decreased with the increase of degradation. The degradation of test material may be determined nondestructively by the lineality of Vickers hardness and the peak interval of reversible permeability.

性別 및 年齡에 따른 體格과 體力의 特性과 變化에 關한 縱斷的 硏究Ⅱ : Emphasised on College Men of Department of Physical Education 體育專功 大學生을 中心으로

金尙國,姜東洹,張周鎬,金昇一,徐再均,金耕知,孫斗玉,鮮于攝,崔榮烈,尹宇相,金亨燉,方永鎭,朴英震,柳在忠 慶熙大學校 體育科學大學 1989 體育科學論叢 Vol.- No.2

The purpose of this study was to compare the characteristics, change in physique and physical function of college students majoring physical education and Taekwondo (454 males and 64 females of age groups ranging from 18 to 26). The results are as following: 1. The male group of 23yrs old in physical education showed highest values in 7 items(Weight, Chest Girth, Fore Arm Girth, Thigh Girth, Calf Girth, Scapular Skinfold Thickness and Suprailiac Skinfold Thickness). 2. In the Physical function, the male group of 18yrs old showed highest values in Power, Flexibility, and then 21yrs old at Back Strength, Vital Capacity, and 23yrs old at Grip Strength, and 25yrs old at Blood Pressure, respectively. 3. In the physique, the male group of 22yrs old showed highest values in Weight, Chest Girth, Thigh Girth, Calf Girth, and then 24yrs old Upper Arm Girth, Fore Arm Girth, and 19yrs old at Height, Upper Limb Length, respectively. 4. In Physical Function, the 22yrs old showed highest values in Back Strength, Vital Capacity, and then 23yrs old at Side Step, Trunk Extension, but 18yrs old showed lower Values in Grip Strength, Side Step, Vital Capacity and Blood Pressure. 5. The female group of 22yrs old showed highest values in 8 items(Height, Sitting Height, Waist Girth, Upper Limb Length, Lower Limb Length, Skinfold Thickness), and then 18yrs old at 5 items (Weight, Upper Arm Girth, Fore Arm Girth, Thigh Girth, Calf Girth), respectively. 6. In the physical function, the 22yrs old showed highest Values in Grip strength, Back Strength, Power, 5 Minute Run, and 18yrs old at flexibility, Harvard Step Test, respectively. 7. In the change of the physique for two years(1988-1989) in the male group, majoring Physical education the 22yrs old showed increase in 4 items, and the 20yrs old in 3 items, but 18yrs old showed decrease in 3 items. 8. In the change of the physical function for two years in the male group, the 20yrs old showed increase in 4 items, and the 21, 25, 26yrs old in 3 items, but the 19yrs old showed decrease in 6 items, and 18, 22 yrs old in 5 items, respectively. 9. The change of the physique for two years in all female group, of 18, 19, 20, 21yrs old showed increase in 11 items except Skinfold Thickness. 10. In the change of physical function for two years in female group, the 18yrs old showed increase in 3 items, and then 20yrs old in 5 items, but 19, 22yrs old decreased 12 items, respectively. 11. The highest significant difference in physique between male and female was found in weight(mean Value 17.5%), and the lowest difference was found Thigh Girth, Calf Girth(1,3%), Upper Limb Length, Lower Limb Length(2.5%). 12. The Highest difference in physical function was Strength (mean Value 40%), Vital Capacity (33%), Power(22%), but Lower Trunk Flexion, Foot Balance(3.4%). 13. In the difference of Physique and physical function between physical education major and taekwondo major, the physical education showed higher values in skinfold Thickines (mean Value 16.5%), Weight, Waist Girth(4.7%) and Strength(33%), Endurance(5.6%), but subjects majoring Taekwondo showed higher values in Flexibility and Foot Balance. 14. In all male groups, Waist Girth, Scapular Skinfold Thickness, Back Strength, Sargent Jump, Trunk Extension and Diastolic Blood Pressure were showed a significantly related at 0.001 level.

중증 대뇌반구경색 환자의 사망률과 초기 예후인자

나해리,강우열,정승철,유경호,마효일,이병철 대한노인병학회 2001 대한노인병학회지 Vol.5 No.2