두경부 악성종양 절제술 후 유리피판을 이용한 재건술 40예의 고찰

백정환,손영익,김성민,추광철,하범준,심영목 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.7

Studying the Genetic Diversity and Phenetic Relationships of Porphyra yezoensis Populations in Korea Using Random Amplified Polymorphic DNA (RAPD)

Young-Mog Kim(김영목),Sung-Hwan(엄성환),Man Kyu(허만규) 한국생명과학회 2019 생명과학회지 Vol.29 No.2

김(Porphyra yezoensis)은 김속의 홍조류이다. RAPD (random amplified polymorphic DNA) 마커를 이용하여 한국 내 네 집단의 표현형과 유전적 다양성을 조사하였다. 전체적으로 20 시발체로 김에서 55분절이 관찰되었다. 이들 밴드 중 30개(54.5%)는 다형성을 나타내었다. OPA-18-02 밴드는 낙동 김 집단에서만 증폭되었다. OPA-20-02 밴드는 서천 김 집단에서만 증폭되었다. 이 두 밴드는 특별한 집단을 구별해주는 특이밴드로 판정되었다. 대립유전자좌위의 수(Ae)는 1.161에서 1.293로 평균은 1.366였다. 서천 김 집단이 가장 높은 다형성을 나타내었다(0.163). 다른 집단과 격리되고 조간대에 위치한 낙동 김 집단은 가장 낮은 다형성을 나타내었다(0.092). 샤논의 표현형 다양성(I)은 서천 김 집단이 가장 높았다(0.238). 전체 유전적 다양도(HT)는 0.132(OPA-02)에서 0.420(OPA-19)로 나타났다. 대립유전자좌위에서 유전적 다양성(HS)은 0.059(OPA-18)에서 0.339(OPA-19)였다. 대립유전자좌위에 근거에서 전체 유전적 다양도에서 집단 간 차이(GST)는 0.012(OPA-11)에서 0.762(OPA-18)이였으며 평균은 0.415였다. 이는 전체 변이의 약 42%는 집단 간에서 발견된다는 것을 의미한다. 종 내 다양도의 58.5%는 집단 내에 있었다. 유전자 흐름(Nm)은 0.705로 낮았다. Porphyra yezoensis is a red algal species in the genus Porphyra. The phenetics and genetic diversity of four populations of P. yezoensis in Korea were reconstructed using random amplified polymorphic DNA (RAPD) markers. Overall, 55 fragments were generated among the tested P. yezoensis array with 20 OPERON primers. A total of 30(54.5%) of these bands were polymorphic. The OPA-18-02 band was amplified in the samples of Nakdong population and absent in them of other three populations. The OPA-20-02 band was only amplified in the Seocheon population. Both bands exhibited distinctive patterns in specific populations. The effective number of alleles per locus (Ae) ranged from 1.161 to 1.293 with a mean of 1.366. The Seocheon population had a high expected diversity (0.163). The Nakdong population was an isolated endemic and intertidal zone. Thus the narrow distributed Nakdong population had a low expected diversity (0.092). Shannon’s index of phenotypic diversity (I) of the Seocheon population (0.238) was the highest among all populations. Total genetic diversity (HT) varied between 0.132 for OPA-02 and 0.420 for OPA-19. The interlocus variation of genetic diversity (HS) was 0.059 for OPA-18 and 0.339 for OPA-19. On a per locus basis, the proportion of total genetic variation due to differences among populations (GST) ranged from 0.012 for OPA-11 to 0.762 for OPA-18 with a mean of 0.415, indicating that 42% of the total variation was found among these populations. In an assessment of the proportion of diversity present within this species, 58.5% (100%-41.5%) of genetic variation resided within the populations studied. The Nm was estimated to be low (0.705).

Elevated mRNA levels of DNA methyltransferase-1 as an independent prognostic factor in primary nonsmall cell lung cancer

Kim, Hojoong,Kwon, Young Mi,Kim, Jin Seuk,Han, Joungho,Shim, Young Mog,Park, Joobae,Kim, Duk-Hwan Wiley Subscription Services, Inc., A Wiley Company 2006 Cancer Vol.107 No.5

<B>BACKGROUND.</B><P>Despite many reports about the involvement of DNA methyltransferases (DNMTs) in human cancers, including nonsmall cell lung cancer (NSCLC), the clinicopathologic significance of DNMTs in primary NSCLC remains to be elucidated.</P><B>METHODS.</B><P>The relation between the mRNA levels of DNMTs (1 and 3b) and the promoter methylation of the p16, RARβ2, H-cadherin, GSTP1, RIZ, and FHIT genes and the clinicopathologic features in 102 fresh-frozen tissues and paraffin blocks were retrospectively studied. The mRNA levels of the DNMTs were assessed via semiquantitative reverse-transcription polymerase chain reaction (RT-PCR), and the methylation status of the CpG islands were determined by methylation-specific PCR.</P><B>RESULTS.</B><P>The mRNA levels of DNMT1 and DNMT3b were elevated in 53% and 58% of 102 NSCLCs, respectively. Hypermethylation of p16, RARβ2, H-cadherin, GSTP1, RIZ, and FHIT occurred in 37%, 38%, 34%, 18%, 9%, and 31% of patients, respectively. Univariate analysis showed that elevated DNMT mRNA levels were not significantly associated with the hypermethylation of 6 genes. However, the elevated mRNA levels of DNMT1 were determined to be significantly associated with the hypermethylation of the p16 promoter (odds ratio [OR] = 2.70, 95% confidence interval [95% CI], 1.02–7.15; P = .02), after controlling for age, gender, pack-years smoked, histology, and pathologic stage. The hazard of failure in cases with elevated mRNA levels of DNMT1 was 3.51 (95% CI, 1.18–12.76; P = .02) times higher than that in those without. The elevated mRNA levels of DNMT3b were not ultimately associated with patient prognosis.</P><B>CONCLUSIONS.</B><P>Elevated mRNA expression of DNMT1 may be an independent prognostic factor in NSCLC and CpG island hypermethylation in NSCLC may be maintained by a complex interaction of several factors rather than by a simple transcriptional up-regulation of DNMT1. Cancer 2006. © 2006 American Cancer Society.</P>

Antifungal and synergistic effects of an ethyl acetate extract of the edible brown seaweed Eisenia bicyclis against Candida species

Kim, Ki-Hyun,Eom, Sung-Hwan,Kim, Hyo-Jung,Lee, Dae-Sung,Nshimiyumukiza, Ossiniel,Kim, Dongsoo,Kim, Young-Mog,Lee, Myung-Suk The Korean Society of Fisheries and Aquatic Scienc 2014 Fisheries and Aquatic Sciences Vol.17 No.2

With the continuing demand for new solutions in the development of effective and safe candidiasis therapies, we investigated the efficacy of an antifungal agent from the marine brown alga Eisenia bicyclis. The methanolic extract of E. bicyclis evinced potential antifungal activity against Candida species. The ethyl acetate (EtOAc)-soluble extract from E. bicyclis demonstrated the strongest antifungal activity against Candida species among five solvent-soluble extracts. Indeed, the EtOAc-soluble extract showed minimum inhibitory concentrations (MICs) ranging from 4 to 8 mg/mL. Furthermore, the EtOAc-soluble extract considerably reversed high-level fluconazole resistance of Candida species. The MIC values of fluconazole against Candida species decreased substantially (from 64 to $4{\mu}g/mL$) in combination with the MIC of the EtOAc-soluble extract (4 mg/mL). The fractional inhibitory concentration indices of fluconazole ranged from 0.531 to 0.625 in combination with 4, 2, or 1 mg/mL of the EtOAc-soluble extract against Candida isolates, indicating that these combinations exert a marked synergistic effect against Candida isolates. These findings imply that compounds derived from E. bicyclis can be a potential source of natural antifungal agents against Candida species.

Pancreatic lipase inhibitory stilbenoids from the roots of <i>Vitis vinifera</i>

Kim, Young-Mog,Lee, Eun-Woo,Eom, Sung-Hwan,Kim, Tae Hoon Informa UK Ltd. 2014 International journal of food sciences and nutriti Vol.65 No.1

<P>Bioassay-guided isolation of an aqueous methanolic extract of <I>Vitis vinifera</I> using pancreatic lipase inhibitory activity led to isolation of seven stilbenoids, wilsonol C (<B>1</B>), heyneanol A (<B>2</B>), ampelopsin A (<B>3</B>), pallidol A (<B>4</B>), <I>cis</I>-piceid (<B>5</B>), <I>trans</I>-piceid (<B>6</B>) and <I>trans</I>-resveratrol (<B>7</B>). The structures were established on the basis of NMR and MS spectroscopic data interpretation. All isolates were evaluated for their inhibitory effects on pancreatic lipase, and stilbenoid <B>1</B> exhibited potent inhibitory effect on pancreatic lipase with IC<SUB>50</SUB> values of 6.7 ± 0.7 µM.</P>

Overexpression of β-Catenin and Cyclin D1 is Associated with Poor Overall Survival in Patients with Stage IA-IIA Squamous Cell Lung Cancer Irrespective of Adjuvant Chemotherapy

Kim, Yujin,Jin, DongHao,Lee, Bo Bin,Cho, Eun Yoon,Han, Joungho,Shim, Young Mog,Kim, Hong Kwan,Kim, Duk-Hwan Elsevier 2016 JOURNAL OF THORACIC ONCOLOGY Vol.11 No.12

<P>Conclusions: The present study suggests that simultaneous overexpression of beta-catenin and cyclin D1 may be associated with poor overall survival irrespective of platinum-based adjuvant chemotherapy in stage IA-IIA squamous cell carcinoma of the lung. (C) 2016 International Association for the Study of Lung Cancer. Published by Elsevier Inc. All rights reserved.</P>

Neurologic outcomes of thymectomy in myasthenia gravis: Comparative analysis of the effect of thymoma

Kim, Hong Kwan,Park, Min Soo,Choi, Yong Soo,Kim, Kwhanmien,Shim, Young Mog,Han, Joungho,Kim, Byoung Joon,Kim, Jhingook Elsevier 2007 Journal of thoracic and cardiovascular surgery Vol.134 No.3

<P><B>Objectives</B></P><P>The objectives of this study were to compare the clinical features and the outcomes after thymectomy between patients with and without thymoma and to evaluate the influence of thymectomy on the subsequent clinical course of myasthenia gravis.</P><P><B>Methods</B></P><P>Between 1995 and 2003, 64 consecutive patients underwent thymectomy, and of these, 60 patients were followed up for at least 12 months postoperatively. The study population was divided into 2 groups based on the presence of thymoma. We performed a retrospective analysis to compare the neurologic outcomes of thymectomy between patients with thymomatous myasthenia gravis and those with nonthymomatous myasthenia gravis.</P><P><B>Results</B></P><P>Twenty-four patients had a thymoma. No significant differences were observed between the 2 groups regarding the preoperative severity of myasthenia gravis. There was no significant difference in the follow-up duration between the 2 groups. There was no significant difference in the overall remission rate between the 2 groups (<I>P</I> = .064). The mean time required to reach a remission was 10.6 months and 23.5 months in the thymoma and nonthymoma groups, respectively. The mean duration of remission was 43.1 months and 30.8 months in the thymoma and nonthymoma groups, respectively. In the early phase of follow-up, more patients reached remission in the thymoma group than those in the nonthymoma group (<I>P</I> = .040).</P><P><B>Conclusions</B></P><P>Neurologic outcomes of the thymoma group were no worse than those of the nonthymoma group. It is expected that earlier thymectomy is likely to result in a better prognosis by shortening the disease period, even for patients with nonthymomatous myasthenia gravis.</P>

Piperlongumine derivative, <b>CG-06</b>, inhibits STAT3 activity by direct binding to STAT3 and regulating the reactive oxygen species in DU145 prostate carcinoma cells

Kim, Young Hwan,Yoon, Yae Jin,Lee, Yu-Jin,Kim, Cheol-Hee,Lee, Sangku,Choung, Dong Ho,Han, Dong Cho,Kwon, Byoung-Mog Elsevier 2018 Bioorganic & medicinal chemistry letters Vol.28 No.14

<P><B>Abstract</B></P> <P>Piperlongumine (PL), isolated from <I>Piper longum</I> L., is receiving intense interest due to its selectively ability to kill cancer cells but not normal cells. We synthesized a number of analogues by replacing the cyclic amide of PL with aliphatic amides to explore structural diversity. Compound <B>CG-06</B> had the strongest cytotoxic profile of this series, showing potent effects in human prostate cancer DU-145 cells, in which signal transducer and activator of transcription 3 (STAT3) is constitutively active. <B>CG-06</B> inhibited STAT3 phosphorylation at tyrosine 705 in a dose- and time dependent manner in DU-145 cells and suppressed IL-6-induced STAT3 phosphorylation at Tyr-705 in DU-145 and LNCaP cell lines. <B>CG-06</B> decreased the expression levels of STAT3 target genes, such as cyclin A, Bcl-2, and survivin. Notably, we used drug affinity responsive target stability (DARTS) to show that <B>CG-06</B> binds directly to STAT3, and the reactive oxygen species (ROS) scavenger N-acetyl cysteine (NAC) rescued the <B>CG-06</B>-induced suppression p-STAT3. Our results suggest that <B>CG-06</B> is a novel inhibitor of STAT3 and may be a useful lead molecule for the development of a therapeutic STAT3 inhibitor.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Piperlongumine (PL) derivative with aliphatic amide was synthesized. </LI> <LI> <B>CG-06</B> is showing potent effects in human prostate cancer DU-145 cells. </LI> <LI> <B>CG-06</B> inhibits STAT3 activity through binding directly to STAT3 and inducing ROS. </LI> <LI> <B>CG-06</B> strongly inhibits the cell growth of DU-145 cells compared to PL. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Prognostic significance of histologic classification and tumor disappearance rate by computed tomography in lung cancer

Kim, Dohun,Kim, Hong Kwan,Kim, Seok-Hyung,Lee, Ho Yun,Cho, Jong Ho,Choi, Yong Soo,Kim, Kwhanmien,Kim, Jhingook,Zo, Jae Ill,Shim, Young Mog AME Publishing Company 2018 Journal of thoracic disease Vol.10 No.1

<P>Conclusions: Histologic subtype according to the IASLC/ATS/ERS classification and TDR both correlated with pathologic invasiveness and predicted survival in patients with lung adenocarcinoma with GGO.</P>

Chlorothalonil- Biotransformation by Glutathione S- Transferase of Escherichia coli

Kim, Young-Mog,Park, Kunbawui,Jung, Soon-Hyun,Park, Jun-Ho,Kim, Won-Chan,Joo, Gil-Jae,Rhee, In-Koo The Microbiological Society of Korea 2004 The journal of microbiology Vol.42 No.1

It has recently been reported that one of the most important factors of yeast resistance to the fungicide chlorothalonil is the glutathione contents and the catalytic efficiency of glutathione S-transferase (GST) (Shin et al., 2003). GST is known to catalyze the conjugation of glutathione to a wide variety of xenobiotics, resulting in detoxification. In an attempt to elucidate the relation between chlorothalonil-detoxification and GST, the GST of Escherichia coli was expressed and purified. The drug-hypersensitive E. coli KAM3 cells harboring a plasmid for the overexpression of the GST gene can grow in the presence of chlorothalonil. The purified GST showed chlorothalonil-biotransformation activity in the presence of glutathione. Thus, chlorothalonil is detoxified by the mechanism of glutathione conjugation catalyzed by GST.