Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

대학생들의 인터넷 중독에 영향을 미치는 요인에 대한 연구

권미영 ( Mi Young Kwon ),황지영 ( Ji Young Hwang ),정혜윤 ( Hye Yoon Jung ),송시형 ( Shi Hyeong Song ) 극동대학교 사회복지연구소 2013 글로벌사회복지연구 Vol.3 No.2

The purpose of this research to suggest implications on introduction of public-program for college students` mental health through analysing the factors which impact on internet addiction of college students. The analysing data is questionnaire intended to 453 college students in Kang- Nam Eulji hospital addiction institute. The questionnaire consisted of internet addiction scale (Young-scale), anxiety scale (STAI) and depression scale (BDI). This study reveals three main findings. Firstly, age and grade have an effect on internet addiction. Second, the younger, the lower grade, they are likely to be depressed and anxiety. Third, most of all, the anxiety has the most effect on internet addiction of college students.

학부모기로의 전환에 관한 연구

윤기영,이미경,박민자 서원대학교 교육연구소 2003 敎育發展 Vol.22 No.1

Parents experience a qualitative change in terms of relationship with their hildren when they start to attend the first educational institute. Parents' role as a 'Hakbumo', whose children are attending school, could be quite different from parents' general role at home. Therefore, it might be needed to investigate the 'Hakbumohood' transition process for appropriate parents development and home-institute relationship After the investigation of literature, in-depth interview and discussion was conducted with 7 early childhood education center 'hakbumo', 2 kindergarten teachers, 1 professor. The result of this study, 1) Most parents take they become a 'Hakbumo' as a matter of course. 2) They feel anxiety about their children's adaptation to new circumstances. 3) They feel variety feelings during the 'Hakbumohood' transition. 4) Their feelings and concern are different between pre attending institute and post attending 5) They were influenced wholeness environment So, it might be concluded that developing a individualized guidance and support program for each new ‘Hakbumo’

유치원 특별활동 프로그램 운영분석

윤기영,권미경 서원대학교 교육대학원 2004 교육논총 Vol.9 No.-

The 'Extra Curricular Activities' in kindergarten has been developed rapidly in korea with the recognition of the importance of earlychildhood education. The purposes of this study were to analyze how an extra curricular activities of public kindergarten is operated and to search an concept about 'Extra Curricular Activities' in the kindergarten. And to recommend ideas in order to improvement way of it's problems. As result, The concept or character of 'Extra Curricular Activities' in kindergarten is different from other school's 'Extra Curricular Activities' Because, there is no 'subject' in kindergarten curriculum. and, it also had many problems, for example, curriculum, lecturer, shortage of facility and material, offical business costs, etc for kinderarten 'Extra Curricular Activities'. In conclusion, the 'Extra Curricular Activities' in kindergarten is an important and useful part on developing abilities of young children. There fore to improve the 'Extra Curricular Activities' in kindergarten and more positive supports for developing 'Extra Curricular Activities' curriculum are required.

DHTML 관점에서 본 Cross-Browser 지원 전략

윤영미 京畿專門大學 2001 京畿專門大學 論文集 Vol.- No.29

If DHTML(Dynamic HyperText Markup Language) application must run on both Netscape and Microsoft browsers, the developers have a choice of several deployment strategies to pursue: page branching, internal branching, common denominator design, and custom API(Application Programming Interface) development. In general, the application will employ a combination of these techniques to get the same results on both browsers. No matter techniques are being used, the developers must know the capabilities of each browser to provide equivalent experiences for users of both browsers.

학교 통일교육지침에 입각한 유치원의 통일교육 구현 방향 모색

윤기영,이미숙 서원대학교 교육연구소 1995 敎育發展 Vol.1995 No.14

The purpose of this study was to investigate how the kindergarten education for reunification in Korea should be, based on the 「Guides for School Teacher」published by Education Ministry. Necessity, appropriateness, and possibility of this education were considered. The results of this study were as follows; Education for reunification in Korea must be done in kindergarten as well as inordinary school because reunification of Korea is essential for us. Also many concepts and characters related with this education are formed and developed in early childhood. And it is one of reasons that early childhood deucation is very important as well as known. So, for successful this education systematic programs should be developed, and education about reunification should be reinforced for kindergarten teacher. Howerer, for the time being, since new programs are not yet developed it is desirable that the current programs such as 「human Education」, 「Democratic Citizenship Education」,「Education for peace」 be used instead to achieve this goal.

대학생을 위한 예비 부모 교육 프로그램 개발을 위한 연구

윤기영,전효숙,박상임,이미숙,이석란,박수옥 서원대학교 학생생활연구소 1998 학생생활연구 Vol.16 No.-

The purpose of this study are to examine reference materials, existing lecture analysis, investigate how student response about new lecture model and to project the basic direction of pre-parent education program. The result of this study are as follows ; <Lecture Model : Parent and Child> Sequence Theme 1 Introduction, Positive Self-Concept 2 Love and Sex 3 Friends versus Lovers 4 Marriage 5 Parenting and Parent's Role 6 Pregnancy and Embriology 7 Brith 8 Middle Term Exam 9 Parent's Role for Infant and Toddler 10 Parent's Role for Child 11 Parent's Role for Youth 12 Society and Environment for Child Care 13 Communication for Parent-Child 14 Problem solving method for Child Care 15 Parent in Future 16 Final Exam

비가열 축합에 의한 포도당 정량법

윤규태,이미영 순천향대학교 기초과학연구소 2002 순천향자연과학연구 논문집 Vol.8 No.1

Glucose concentration was determined by using the modified method of o-toluidine /boric acid condensation method. Instead of heating in the presence of acetic acid in o-toluidine /boric acid condensation method, NaOCl, H₂O₂or ClC_(6)H₄CO₃H was used to stimulate the formation offufural derivative. The r values of calibration curve for glucose determinatiion by using H₂O₂, NaOCl, and ClC_(6)H₄CO₃H without heating were 0.97, 0.99 and 0.76, respectively. These results suggest that NaOCl might be the most effective condensation stimulator in glucose determination in terms of high r value when non-heating condensation method was used.

서울지역 주부들의 혼례음식에 대한 인지도 조사

윤숙자,이미영 한국식생활문화학회 2002 한국식생활문화학회지 Vol.17 No.5

본 연구는 서울 지역의 주부를 대상으로 하여 혼례음식(폐백과 이바지 음식)의 인식상황과 실태를 조사함으로써 혼례음식 발전의 기초자료를 제공하고자 얻은 결과를 요약하면 다음과 같다. 첫째, 폐백음식의 인식에 대해서는 "결혼 후 시댁에 인사를 드리는 것"이라는 응답이 55.2%, "옛날부터 내려오는 하나의 풍습"이 35.8%로 나타나 상당히 정확하게 알고 잇는 것으로 나타났다. 폐백음식의 중요도 인식수준은 전체 응답자의 10.2%가 "매우 중요하다"라고 응답하였으며, "중요하다" 48.5%, "중요하지 않다"는 11.9%로 나타나 폐백음식의 중요성에 대하여 공감하는 비율이 높게 나타났다. 특히 주부의 연령별로 20대 집단에서 30대 이상의 집단에 비해 "중요하지 않다"는 응답율이 높게 나타났다.(p<0.05) 둘째, 폐백음식 준비방법으로는 "가정에서 직접 마련"한다와 "시장이나 떡집에서 마련"하겠다는 응답이 비슷한 비율을 보였으며, 연령별·교육수준별(p<0.05)로 유의한 차이를 보였다. 폐백음식 장만시 드는 비용으로는 "30~50만원"이라고 응답한 비율이 50%로 가장 높게 나타났고, "10~30만원"29%>"50~100만원"18% 순으로 나타났다. 셋째, 선호하는 폐백음식으로는 "밤·대구고임"을 준비한다는 의견이 77.5%로 가장 높게 나타났으며, 그 다음으로 "떡류">"육포">"술">"한과" 등의 순으로 나타났다. 넷째, 이바지음식에 대한 의미로는 48.2%가 "신부측에서 마련한 음식을 시댁에 보내는 것"이라고 응답하였으며, 이바지 음식의 필요성에 대해서는 "반드시 준비해야 한다"라는 의견이 15.5%, "형편에 맞추어 준비한다"는 78.7%, "준비할 필요가 없다"5.8%로 나타나 전반적으로 이바지 음식의 필요성에 대해서 공감대를 형성하고 잇는 것으로 나타났다. 또한 취업주부가 "필요하다"라는 응답이 높게 나타났고, 종교적으로도 불교를 믿는 주부보다 기독교를 믿는 주부들에게서 유의적인 차이를 보였다.(p<0.01) 다섯 번째, 혼례음식의 개선점으로 메뉴의 간소화>가격을 저렴하게 하는 문제>폐백음식 선택의 다양화 등으로 나타났다. 연령별로 40대 주부집단에서 "메뉴의 간소화" 응답율이 높게 나타났다.(p<0.01) 이상의 같은 연구 결과로 보아 우리나라의 모범이 되는 혼례음식의 계승을 위해서 꾸준한 노력과 관심이 필요하다고 사료된다. 과거와 다른 신식혼례를 치르면서도 폐백을 드리고 이바지 음식 보내는 풍습은 계속 이어지고 있다. 이러한 혼례 풍습은 계속 이어질 것이기에 혼례음식이 가지고 잇는 기복과 주술적인 의미를 되살리면서 저렴한 가격으로 누구나 손쉽게 장만할 수 잇는 간편한 방법이 모색되어 전통을 살리면서 모범이 되는 혼례음식문화를 계승 발전시켜 나가야 하겠다. This study was carried out to survey the degree of the recognition and necessity of wedding food to include Pha`ebak and Ibaji foods prepared by bride's parents and sent to the bridgroom's. The participants of the survey were 293 housewives of 20 years old or more residing in Seoul area. The questionnaires used in this research consisted of 10 questions. The findings could be summarized as follows: 1. As to the degree of the recognition of Pha`ebak food, 55.2 percent of the participants perceive it as 'the food sent to the bridegroomvs house to be used when the newly-weds greet groom's relatives, whereas 35.8 percent regarded the practice as "a traditional custom from the ancient times." which is, in fact, correct and exact. As to its importance, 10.2 percent regarded Pha`ebak food very important, while 48.5 percent, "important," and 11.9 percent, "not important." 2. As for the items for Pha`ebak dishes, 77.5 percent answered their preferences for "jujube prop-nut," followed by "ricecake," "liquor" and "Korean cookies," in descending order. 3. As for the meaning of Ibaji dishes, 48.2% understand them as "a set of dishes prepared by the bride's parents to be sent to the bridegroom's"; and as for their willingness or necessity of sending the Ibaji dishes, 15.5 percent indicated that they might prepare them, whereas 78.7 percent said that they would prepare them if the situation required. On the other hand 5.8 percent did not feel the necessity. The overall result is that most females surveyed recognized the two types of food important and were inclined to prepare them when the occasion demanded.