섬유소 분해군 Cellulomonas uda CS 1-1의 분류학적 연구

김미석,윤민호,최우영 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

Cellulomanas sp. CS 1-1 was studied for its morphological, physiological and biochemical characteristics, together with DNA homology and fatty acid pattern to elucidate its taxonomical position in the species level. Colony morphology of CS1-1 exhibited circular form, opaque, convex, entire edge and pale yellow. Cells were of rod with the size of 0.3∼0.5 × 0.8∼1.2 ㎛, while coryneforms were formed at the early stage of culture. D-ribose, raffinose, rhamnose, acetate, propionate, L-lactate, D-gluconate, aspartate and proline were not utilized as a sole source of carbon, whereas saccharose, arabinose, and amlyose were utilized. Biochemical characteristics of CS1-1 were Gram positive, catalase positive, oxidase negative, nonmotile, facultative anaerobic, mesophilic and G+C content of 74.7 mol %. The major fatty acid and menaquinone were 12-methyltetradecanoic acid(anteiso-C_(15:1)) and MK-9(H₄), respectively. These results were correspondent with the characteristics reported for member of the genus Cellulomonas. The strain CS 1-1 exhibited a high level of DNA homology as 70% with C. uda ATCC491, compared to those of 54∼59% with C. fimi ATCC 15724, 46∼48% with C. biazotea, C. gelida and C. bibula. Finally, strain CS1-1 could be classified as a novel species belongs to C. uda. 섬유소 분해균 Cellomonas sp. CS 1-1에 대하여 종 수준으로 분류학적 위치를 규명하기 위하여 7개 type strain 균주와 함께 생리적 및 생화학적 특성을 조사하고 DNA 상등성 및 지방산의 조성 등을 분석하여 비교한 결과, CS 1-1의 콜로니 형태는 circular, entire, smooth convex하며, 담황색을 띤 0.3∼0.5 × 0.8∼1.2 ㎛ 크기의 간균이었다. 생리학적 특징으로서 비운동성의 통성혐기성·중온균으로서 Gram양성, catalase양성, oxidase음성, 탄수화물 발효성 등의 표현형은 Cellulomonas 속의 타종과 동일하였으며, 특히 D-ribose, raffinose, rhamnose, xylitol, acetate, L-lactate, propionate, aspartate, proline 등 조사한 모든 탄소원의 이용성이 없었으며, 반면에 sacchrose, arabinose 및 amylose의 이용성은 양성으로 판정되었다. G+C 함량 74.76 mol %, 주요 지방산과 quinone 성분은 전형적인 Cellulomonas의 12-methyltetradecanoic acid (anteiso-C_(15:0))과 MK-9(H₄)이었으며, DNA의 상동성 비율은 C. uda ATCC 491과 70%, C. fimi ATCC 15724와 54∼59 %, C. gelida 및 C bibula와도 46∼48%의 상동성을 나타내었다. 이상의 결과는 CS1-1이 현재 Cellulomonas 속에 인정된 7개의 type species 중 C. uda ATCC 491 균주와 가장 높은 근연성을 나타냄으로서 C. uda에 속하는 novel species로 분류될 수 있다.

우유와 두유를 혼합한 이유보충음료의 개발

손경희,윤선,이민준,김순영,김용태,장동훈,강석필 연세대학교 생활과학연구소 2001 生活科學論集 Vol.10 No.1

The purpose of this study is to develop an infant supplement composed of cow and soy milk which has acceptable taste and digestability and meets nutritional needs of the infants. Even though soy milk is and excellent source of protein for infants who are allergic to dairy products, its protein quality is still lower than that of regular milk. Therefore in the present study the development of sow-soy milk infant formula was attempted. To make the formula equal amount of cow and soy milk were mixed and supplemented with vitamins and minerals to meet the nutritional standards of breast milk, CODEX and recommended dietary allowances for Korean infants. In animal experimental study, the weight gain rate of weaning rats was highest in the order of 100% milk, cow-soy milk, control, soy milk, commercial soy infant milk group. Feed efficiency ratio(FER) were not significantly different among control, 100% milk and cow-soy milk groups. However, commercialized soy infant milk group showed significantly lower FER than other groups. When the cow-soy milk formula was fed to infant in the age of 5 month to 25 months, it did not show any unsatisfactory results in acceptability and bowel movements of the subjects. Since the cow-soy milk formula has not only all the nutrients for the physical growth of infants but also contains valuable physiological functional ingredients, it is expected to be a good choice of supplements for infants.

A Study on Improvement Strategies of the Measuring Instrument Industry in Korea

Yoon, Seog Ki,Lee, Byung Min 한국생산성학회 1997 THE JOURNAL OF PRODUCTIVITY Vol.3 No.1

This study is to analyze the domestic/foreign trend of demand and supply, and to suggest development plan of the measurement instrument industry in Korea. Advanced countries have steadily developed industrial technology over the past 200 years, and accordingly measuring technology was already recognized as an essential part for industrial development. In case of Korea, most of industrial technology was introduced from abroad as one of industrialization-drive policy in 1960s, which as a result impeded development of measuring technology which is based on its own capability. Recently, recognizing the importance of measuring instrument, Korea has been developing various kinds of measuring instruments in a small amount, but turned out to be inefficient for development of measuring instrument technology. And thus, it is requested to estimate the coming demand of measuring instruments and to create appropriate long-term goals accordingly. Above all, an improvement strategy should be drawn up in the long-term point of view for coping with the increasing demand of measuring instrument and securing competitiveness in the world market.

소장 장간막의 낭종성 림프관종에 의한 소장 염전 1예

하창윤 ( Chang Yoon Ha ),심기남 ( Ki Nam Shim ),강민정 ( Min Jung Kang ),정지민 ( Ji Min Jung ),나윤주 ( Youn Ju Na ),정성애 ( Sung Ae Jung ),유권 ( Kwon Yoo ),민석기 ( Seog Ki Min ) 대한장연구학회 2008 Intestinal Research Vol.6 No.1

A cystic lymphangioma is a rare benign tumor that arises in an organ with a large number of lymphatics. It arises in patients of all ages and has variable presentations. An abdominal cystic lymphangioma most commonly occurs in the intestinal mesentery, with the retroperitoneum being the second location of choice, and it causes abdominal pain, hematochezia, intussusceptions and protein-losing enteropathy. If the cystic lymphangioma presents with symptoms, surgical excision is required for treatment and diagnosis. We report here a case of cystic lymphangioma of the small bowel mesentery that presented with a small bowel volvulus, along with a brief review of the literature. (Intest Res 2008;6:76-79)

Characterization of Anti-complementary Polysaccharide from Teucrium viscidum var. miquelianum

Min, Jin-Gi,Kim, Tae-Jin,Lee, Doo-Seog,Ch, Sang-Won,Yoon, Ho-Dong,Park, Jeong-Heum The Korean Society of Food Science and Nutrition 2001 Preventive Nutrition and Food Science Vol.6 No.3

Water-soluble crude polysaccharide (TM-1) prepared from the leaves of Teucrium viscidum var. miquelianum was fractionated into three polysaccharide fractions, TM-2, TM-3 and TM-4 by the addition of cetyltrimethylammonium bromide. The major polysaccharide fraction, TM-2, consisted of glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, and all fractions contained galactose and glutose as the major neutral sugars. TM-4 showed the highest anti-complementary activity. When TM-4 was futher fractionated by anion exchange chromatography, TM4- II a and TM4-II b showed the most Potent anti-complementary activity. TM4- II a was composed mainly of galactose, arabinose and glucose in the molar ratios of 2.13 : 0.94 : 1.00 respectively, and contained a small amount of galacturonic acid and glucuronic acid.

Characterization of Streptomyces sp . AMLK - 135 Producing Anti - MRSA Antibiotics

( Min Jeong Lee,Dae Seog Lim,Myung Sub Lee,Won Ho Yoon,Chang Han Kim ) 한국미생물생명공학회 1997 Journal of microbiology and biotechnology Vol.7 No.6

Effect of Reaction Conditions on Pore Configuration and Mechanical Property for Porous Hydroxyapatite Prepared by Polymer Sponge Method

Min, Sang Ho,Jin, Hyeong Ho,Jun, B.S.,Park, Ik Min,Park, Hong Chae,Yoon, Seog Young Trans Tech Publications, Ltd. 2007 Key Engineering Materials Vol.336 No.-

<P>Porous HAp scaffolds have been prepared by using the slurry including HAp and magnesia based on the replication of polymer sponge substrate. The influence of HAp and MgO content in slurry on the pore morphology and size, and density, porosity, and mechanical strength of porous HAp scaffolds was investigated. The obtained scaffolds with average pore sizes ranging 150 to 300 μm had open, relatively uniform, and interconnected porous structure regardless of HAp and MgO content. As the MgO content increased, the pore network frame of scaffolds became to be relatively stronger, even though the pore size was not much changed. The compressive strength of the scaffolds increased rapidly with the increase of MgO content at a fixed HAp content because of increasing the pore wall thickness and density of the scaffolds. As a result, the porosity, density, and compressive strength of the porous HAp scaffolds scaffolds prepared by the sponge method were significantly affected by the addition of MgO.</P>

Case Reports : Malignant Peripheral Nerve Sheath Tumor Arising from Neurofibromatosis - Electron Microscopic Study -

( Seog Jun Ha ),( Young Min Park ),( Jong Yuk Yi ),( Tae Yoon Kim ),( Chung Won Kim ),( Jung Yong Lee ),( Seok Jin Kang ) 대한피부과학회 1996 Annals of Dermatology Vol.8 No.2

Laparoscopic Treatment for Intrahepatic Duct Stones in the Era of Laparoscopy: Laparoscopic Intrahepatic Duct Exploration and Laparoscopic Hepatectomy

Yoon, Yoo-Seok,Han, Ho-Seong,Shin, Sang-Hyun,Cho, Jai Young,Min, Seog Ki,Lee, Hyeon Kook Lippincott Williams Wilkins, Inc. 2009 Annals of surgery Vol.249 No.2

OBJECTIVE:: The aim of this study is to analyze our experiences with laparoscopic surgery for treating intrahepatic duct (IHD) stones and to evaluate its role for the management of IHD stone. SUMMARY BACKGROUND DATA:: Until now, laparoscopic surgery for IHD stone has been rarely reported. METHODS:: From October 1998 to June 2007, we performed 76 cases of laparoscopic surgery for treating IHD stones [30 laparoscopic IHD explorations (LIHDE) and 46 laparoscopic hepatectomy (LH)]. The choice between LIHDE and LH was based on the severity of the IHD stricture, the presence of parenchymal atrophy, and the impaction of stones, as determined by the preoperative radiologic images or intraoperative choledochoscopy. Retrospective analysis was done on the clinical outcomes of the 76 patients. RESULTS:: Conversion to open surgery was needed in 6 patients (7.9%). The mean operation time (LIHDE vs. LH) was 278.4 and 344.3 minutes, respectively. The mean postoperative hospital stay was 14.6 and 12.8 days, respectively. Postoperative complications occurred in 28 patients (36.8%), and all of them responded to the conservative management. There was 1 case of postoperative mortality in the LH group because of sepsis. The overall initial success rate of removing the stones by laparoscopic surgery in our intention-to-treat analysis was 78.9% (60 of 76). The reasons for treatment failure included remnant stones (n = 9), conversion to open surgery (n = 6), and postoperative mortality (n = 1). For the 69 patients who had laparoscopic surgery successfully preformed without mortality, the initial success rate of stone clearance was 87.0% (60 of 69) and the final clearance rate after additional choledochoscopic stone removal was 92.8% (64 of 69). CONCLUSIONS:: This study demonstrates that laparoscopic surgery can be an effective option for managing IHD stones and it also suggests the potential role of laparoscopic surgery for treating IHD stones in the era of laparoscopy.

Preparation of Porous Hydroxyapatite Scaffolds for Bone Tissue Engineering

Min, Sang Ho,Jin, Hyeong Ho,Park, Hoy Yul,Park, Ik Min,Park, Hong Chae,Yoon, Seog Young Trans Tech Publications, Ltd. 2006 Materials science forum Vol.510 No.-

<P>Porous hydroxyapatite (HAp) scaffolds were successfully prepared by using the HAp slurry based on the replication of polymer sponge substrate. The effect of HAp content in slurry on the pore morphology and size, and density, porosity, and mechanical strength of porous scaffolds was investigated. The scaffolds with average pore sizes ranging from 200 to 400 µm had an open, relatively uniform, and interconnected porous structure. As the HAp content increased, the porosity of scaffold decreased while the density increased. These phenomena were attributed to the fact that the pores became interconnected with more dense and thicker pore walls with increasing HAp content in slurry. The results suggest that the density, porosity, and compressive strength of the porous HAp scaffold were significantly affected by the content of the HAp powder in the slurry.</P>