고관절 치환술후 무균성 해리와 골용해 조직에서 세포사멸

김신윤,인주철,손윤경,김정완,신홍인,구경회 경북대학교 병원 2003 경북대학교병원의학연구소논문집 Vol.7 No.1

목적 : 인공 고관절 치환술 후 실패의 가장 중요한 원인이 되는 무균성 해리및 골용해와 nitric oxide(NO)에 의한 염증세포 사멸의 연관성을 규명하기 위해 본 연구를 시행하였다. 대상 및 방법 : 무균성 해리와 골용해로 인해 재치환술을 받은 18명의 환자의 조직에서 세포들의 사멸을 Terminal Deoxyribonucleotidyl Transferase-Mediated dUTP Nick End Labeing (TUNEL) 방법을 이용하여 검사하였으며, inducible nitric oxide synthase(iNOS)와 미토콘드리아 관련 세포사멸 경로를 p53, Bax, Bcl2, Ki67 항체를 이용해서 면역 염색하였다. 대조군으로 대퇴 경부 골절 환자 6명의 관절낭을 이용하였다. 결과 : 16명(89%)에서 iNOS, p53, Bax 항체에 양성 반응을 보였으며, 12명(67%)에서 Bcl2, Ki-67 항체에 양성반응을, 전례에서 TUNEL에 양성반응을 보였다. 대조군에서는 1명(17%)에서 iNOS와 Bax에, 3명(50%)에서 TUNEL에 양성반응을 보였다. 무균성 해리와 골용해 조직의 세포사멸에 대한 양성반응의 발현빈도가 대조 조직에서 보다 유의하게 높았다. 결론 : Nitric oxide에 의한 염증세포의 사멸이 인공고관절 치환술 후 생기는 무균성 해리와 골용해에 작용함을 알수 있었다. 이 결과는 해리 및 골용해의 예방과 치료방법을 개발하는데 도움을 줄 수 있다. Purpose : To evaluate the relationships between the apoptosis induced by nitric oxide(NO), aseptic loosening and osteolysis, which are the most serious causes of failure after total hip arthroplasty. Materials and Methods : Apoptosis of the inflammatory cells of interface tissues from 18 patients who underwent revision hip arthroplasty was identified by Teminal Deoxyribonucleotidyl Transferase-Mediated dUTP Nick End Labeling(TUNEL). The reaction to immunostaining of inducible nitric oxide synthase(iNOS), p59, Bax, Bcl2, and Ki-67 were evaluated. Six joint capsules obtained from six patients of femoral neck fracture were studied as controls. Results : Sixteen(89%) of 18 interface tissues were positive for iNOS, p53, and Bax monoclonal antibody and twelve(67%) for Bcl2 and Ki-67 monoclonal antibody. All tissues were positive for TUNEL. In the control group of six joint capsules, only one(17%) was positive for iNOS and Bax, and three(50%) were positive for TUNEL. The incidences of apoptiotic signals at the interfacial tissue of aseptic loosening and osteolysis were significantly greater than those of the control. Conclusion : The current study suggested that the apoptosis of inflammatory cells, due to oxidative stress by NO, might be involved in the development of implant loosening and osteolysis after total hip arthroplasty. This information might be crucial for the treatment and prevention of periprosthetic osteolysis and subsequent loosening.

A case of lichen amyloidosis in an adult

( Jee Yon Shin ),( Myeong Heon Chae ),( Ji Yeoun Lee ),( Tae Young Yoon ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.1

Lichen amyloidosis is a type of primary localized cutaneous amyloidosis clinically characterized by persistent pruritic, hyperkeratotic papules commonly distributed on the shins. Histopathologically, amyloid deposition in the papillary dermis is the characteristic finding. It appears to be more common in males and most often occurs in people 50-60 years of age. A 49-year-old woman presented with brownish papuloplaques on the both lower legs. Itching sense was severe and the lesion was occurred 2 years ago. Histopatholgic findings showed amyloid deposit in the papillary dermis. D-PAS staining was negative. Based on these findings, a diagnosis of lichen amyloidosis was made. We think this case has typical chracteristics of lichen amyloidosis. Therefore we report a case of lichen amyloidosis for educational purposes.

막증식 사구체신염으로 발현한 루푸스 신염 1례

윤신애, 이호창, 하태선 충북대학교 의과대학 충북대학교 의학연구소 2012 忠北醫大學術誌 Vol.22 No.1

전신성 홍반성 낭창(systemic lupus erythematosus, SLE)은 비정상적으로 증가한 병원성 자가 항체와 면역복합체에 의한 조직 및 세포의 손상을 특징으로 하는 자가 면역성 질환으로, 다양한 자가 항체와 면역복합체는 신장을 포함한 모든 장기를 침범할 수 있다. 신장을 침범한 전신성 홍반성 낭창은 단백뇨, 현미경적 혈뇨, 고혈압과 신기능 이상을 발현할 수 있다. 저자들은 초기 임상적으로 막증식 사 구체신염(membranoproliferative glomerulonephritis, MPGN)의 임상소견으로 발현하여, 검사 상 전신성 홍반성 낭창과 조직학적으로 MPGN 소견을 보인 여자 환아를 경험하였기에 문헌고찰과 함께 보고하는 바이다.

齒牙成分(layer sample)內 有機質,枸椽酸 및 乳酸含量의 年齡的變化와 齒髓의 生物學的 硏究

尹信鉉 醫藥係社 1963 綜合 醫學 Vol.8 No.6

인도메타신이 개의 유치 치근 흡수에 미치는 영향

신강섭,강윤구,이기수 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.2

이 연구는 흡수중인 유치를 대상으로 인도메타신이 치근흡수에 미치는 영향을 조사하고, 치근흡수에 관련된 주위 조직의 변화를 관찰하기 위하여시행되었다. 인도메타신은파골세표의 수를 감소시키고 골흡수를 감소시키며, 골형성도 감소시키는 것으로 보고되어 왔으나 형태와 기능이 유사하다는 파치세포에 미치는 영향에 관한 연구는 희소하다. 생후 12-13주된 잡견 6마리를 통상적으로 복용량인 인도메타신 2㎎/㎏/day를 14일간 투여한 군과 과량의 8㎎/㎏/day를 14일간 투여한 군과 대조군으로 구분하였으며 흡수중인 하악 절치를 연구대상으로 하였다. 연구대상 치아는 5㎛ 두께의 절편을 만들고, H & E 중염색. Masson의 trichrome 염색을 시행하고 광학현미경으로 검경하였으며,파치세포의 수와 핵의 수를 비교하였다. 관찰 결과 유치 치근 흡수 조직은 골개조 소견과 함께 염증 소견과 유사한 소견을 보였다. 흡수중인 유치의 치수는 치근흡수부위에 가까운 조상아세포층은 변성의 소견을 보이나 멀리 떨어진 치수는 정상인 소견을 보였으며 인도메타신이 투여된 실험군에서는 파치세포의 수적 감소와 핵의 수적 감소를 미약하게 나타냈다. 그러나 인도메타신이 흡수중인 치아의 치수에 미치는 영향은 관찰되지 않았다. 이상의 결과에 의하면 인도메타신은 파치세포의 수적 감소를 미약하게 일으키며, 장기간의 인도메타신 투여는 유치 치근흡수의 지연을 초래할 가능성이 있음을 시사한다. The study was aimed to invesigate the effects of indomethacin on physiologic root resorption and to examine the dental pulp and tissue changes around the resotbing teeth. 13-14 week old six mongrel dogs were divided into 3 groups: two experimental groups administered indomethacin 2㎎/㎏/day and 8㎎/㎏/day orally two times a day for 14 days respectively, and control group administered a placebo. The deciduous incisors showing root resorption were selected, fixed for 24hrs in 10% formalin solution, demineralized in 10%EDTA solution, invested in paraffin and sectioned in 5㎛ thick sections. The preparations were staine with H & E staining and Masson's trichrome staining and examined unger the light microscope. Observation revealed that deciduous root resorbing tissue resembles inflammatory tissue and accmpanies bone remodelling. The dental pulp was normal except the area near root resorption, well organized columnar odontoblasts layer under the predentin, and the odontoblasts near root resorption were cuboidal or flat cells in the disrupted layer under the predentin. Indomethacin administered group showed a partial decrease in the number of odontoclasts and nucleus. But there was no sign of pulp change by indomethacin. These results suggest that indomethacin inhibits recruitment of odontoclasts partially and that of osteoclasts more, and so when it is administered for long periods, deciduous root resorption can be delayed and eruption of the successor can be delayed for a short period.

건식경량벽체의 내충격성능 평가방법에 관한 연구 (Ⅰ) : 인간이 벽체에 가하는 충격하중의 분석을 위한 실험 및 고찰 An Experiment to Analyze the Human Impact Load Applied to Framed Walls

신윤호,안정업,김대희,최수경 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

The purpose of this study is to establish an evaluation method of fracture of light-weight framed walls by human impact load. In this paper, part (I), we analyzed human impact load applied to framed walls. At first, we made original measuring equipment of impact load, and carried out the experiment on human impact load in various action. After the experiment, we extracted the essential characteristics. And grasped the property of human impact load systematically based on the relation between maximum load and load velocity.

슬라이딩 모드를 이용한 LDM 서보밸브의 추종제어

윤신용,백수현 동국대학교 산업기술연구원 1999 산업기술논문집 Vol.13 No.-

본 논문은 LDM을 이용한 직접구동 서보밸브의 위치 추종제어를 연구하였다. 선형 전자석 솔레노이드로 구동되는 노즐 플레퍼형 서보밸브와 비례 선형 밸브는 공장 자동화 분야에 광범위하게 적용된다. 이러한 고전적인 서보밸브는 효율, 오염, 저속에서 문제점을 지니고 있기 때문에, 고속 및 고정밀 서보밸브가 요구된다. 따라서 본 연구에서는 LDM을 이용한 고속 하이드라릭 서보밸브를 적용한다. 제어는 슬라이딩 모드인 새로운 형태의 상태 공간 궤적을 이용한다. 가변구조시스템 (슬라이딩모드)은 적합한 스위칭 로직과 함께 연속적 특성을 갖는 종속시스템으로 가변구조시스템을 구성하고 있다. 따라서 본 연구에서는 슬라이딩모드 제어기법을 이용한 최적의 성능을 갖는 시스템을 제안한다. This paper deals with a position sliding mode control of direct drive hydraulic servo valve(linear servo valve, LSV) using linear DC motor (LDM). The nozzle-flapper type servo valves and proportional linear valves driven linear electromagnetic solenoid are widely used in factory automation. Because these conventional valves involve problems in efficiency, contamination and low-speed. High speed and hi precision servo valves have been required. This paper is applied to high speed hydraulic servo valve using LDM. The controller is designed so that a new type of state space trajectories called sliding mode exists. Variable structure system (sliding mode) consist of a set of continuous subsystems together with suitable switching logic. Therefore, a sliding mode control has the control performance very near to the time optimal one.

아스퍼거장애와 비전형 자폐장애 아동의 KEDI-WISC와 BGT 수행의 비교

신민섭,양윤란 대한소아ㆍ청소년정신의학회 1998 소아청소년정신의학 Vol.9 No.2

만성 화농성 중이염의 세균학적 고찰

윤신의,문문만,김승찬,박희완,나한조,최봉남 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

The chronic suppurative otitis media is one of the most common disease in the otologic field. And the works on bacterial distribution of chronic suppurative otitis media were studied by many otologist, but there is some difference. The bacteriologic study was done on 126 cases of chronic suppurative otitis media who visited the Department of Otolaryngology, Chosun University Hospital between January 1989 and December 1989 and the following conclusions were obtained. 1) In sex distribution, the male had higher incidence rate than female (M: 56.3%, F:43.7%). 2) In age distribution, 3rd decade (34.1%), 2nd decade (34.1%), were most prevalent. 3) In duration of the disease, the hightest frequence was below 5 years duration 4) Among 126 cases, 98 cases(77.8%) were unilateral (right ear was 47 cases(37.3%) and left ear was 51 cases(40.5%))and 28 cases(22.2%) were bilateral. 5) The 136 cases(88.3%) showed the positive results under culture, but 18 cases(11.7%) didn't growthed. Among 136 cases 95 cases(61.7%) had single infection and the 41 cases(26.6%) had mixed infection. 6) The most frequently isolated bacteria was staphylococcus(38 strains, 27.9%) and next were proteus(27 strains, 19.9%) and pseudomonas (22 strains, 16.2%). 7) The Staphylococcus was sensitive to cephalothin(94.7%), amikacin(78.9%) and resistant to penicaIin(63.2%). 8) The Proteus was sensitive to amikacin(92.6%), cephalothin(88.9%) and resistant to minocycline(81.5%) and gentamicin(70.4%). 9) The Pseudomonas was sensitive to amikadn(86.4%), gemtamicin(59.1%) and resistant to minocycline(90.9%), ampicillin(86.4%) and cephalothin(86.4%).

전신성 홍반성 낭창의 임상적 고찰

신영태,김종학,장윤경,양종오,구영선,강민규,황평주,나기량,이강욱,서광선 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

The epidemiology, diagnostic criteria, clinical features, symptoms and signs laboratory findings, kidney pathology, and clinicopathologic correlation of systemic lupus erythematosus(SLE) were analyzed. The 63 patients studied were managed at the Department of Internal medicine, CNUH, from January 1983 to December 1997. Kidney biopsy was performed in 53 patients out of 63 patients with SLE. The results were as follows: 1. The ratio of male to female was 1:26. They were 12 to 71 years old and mean age was 32.1 years old. The peak age incidence was 4th decades(30%). 2. The most frequent chief complaint on admission was generalized edema. Most patients complain two or more symptoms. 3. Immunologic and renal disorders were the most frequently observed in the ARA criteria of SLE. And the positive ANA, hematologic disorder, malar rash, and arthritis, were observed in order of frequency. 4. Among the 53 patients with renal biopsy, 30 patients revealed class Ⅳ lupus nephritis(56%), class II in 12 patients(23%), class V in 8 patients(15%) and class III in 2 patients(6%). 5. The cases of lupus nephritis represented as nephrotic syndrome were high in the class IV with 68% and class V with 86%. 6. Of 30 patients who can be followed up, 5 patients resulted in death(17 % of mortality). They "were 14 to 57 years old and mean age was 28 years old. Follow up duration were from 1 month to 6 years and 3 months, and mean duration was 1 year and 1 month, but 3 cases 7. The causes of death were as follows: Two cases were due to seizure and coma resulted from CNS involvement. One was sepsis due to miliary the & pneumonia. One was dilated cardiomyopathy and heart failure due to cardiac ac involvement. And the other one was sepsis and acute renal failure resulted from cellulitis. 8. Prednisolone was used in all patients basically, and methyl-prednisolone pulse therapy, antimalarials and alkylating agents were used in some cases. In the cases of combined therapy, the activity of SLE was well controlled.