Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

대장균의 전사조절 유전자 nlp의 분자기구 해석

최용락,정수열,정영기,정정한 동의대학교 기초과학연구소 1994 基礎科學硏究論文集 Vol.4 No.1

An nlp (Ner like protein) gene from E. coli was previously cloned and sequenced. Here was show that expression of the sugar metabolism related genes, lacZ, malQ, and malP, increased 2.5- to 8.3-fold in the presence of a plasmid containing the nlp gene. This suggested that the nlp gene could induce maltose- and lactose-metabolism coordinately with crp*1 in the absence of cAMP. Using the nlp-lacZ fusion gene, it was possible to show the promoter of nlp was active in vivo. The overexpressed nlp gene product, a polypeptide of 10,000 daltons, was confirmed by SDS-polyacrylamide gel electrophoresis. The band shift assay revealed that the partially purified Nlp protein bound a specific DNA of the regulatory region of the nlp gene.

1~2기 본태성 고혈압 환자들에서 Imidapril 대 Enalapril의 항고혈압 효과를 평가하기 위한 무작위, 이중맹검 임상시험

최영진,손대원,김용진,이홍자,채인호,김효수,김철호,오병희,이명묵,박영배,채윤식,이영우 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.11

하악 제3대구치와 하악관과의 위치관계에 대한 파노라마 방사선사진과 cone beam형 전산화단층영상의 비교

최형수,김규태,최용석,황의환 대한구강악안면방사선학회 2008 Imaging Science in Dentistry Vol.38 No.3

Purpose : To assess the diagnostic accuracy and value in an imaging technique field through the comparison of cone beam computed tomography and conventional panoramic radiography in assessing the topographic relationship between the mandibular canal and impacted third molars. Materials and Methods : Participants consisted of 100 patients offered the images through cone beam computed tomography and panoramic radiography. PSR-9000 Dental CT system (Asahi Roentgen Ind. Co., Ltd, Japan) was used as the unit of cone beam computed tomography. CE-II (Asahi Roentgen Ind. Co., Ltd, Japan) and Pro Max (Planmeca Oy, Finland) were used as the unit of panoramic radiography. The images obtained through panoramic radiography were classified into 3 types according to the distance between mandibular canal and root of mandibular third molar. And they were classified into 4 types according to the proximity of radiographic feature. The images obtained through cone beam computed tomography based on the classification above were classified into 4 types according to the location between the mandibular canal and the root and were analyzed. And they were classified into buccal, inferior, lingual, and between roots, according to the location between mandibular canal and root. The data were statistically analyzed and estimated by χ²-test. Results : 1. There was no statistical significance according to 3 types (type l, type ll, type lll) through CBCT. 2. The results of 4 types (type A, type B, type C, type D) through CBCT were as high prevalence of CBCT 1 in type A, CBCT 2 in type B, CBCT 3 in type C, and CBCT1 in type D and those of which showed statistical significance (P value=0.03). 3. The results according to location between mandibular canal and root through CBCT recorded each 49, 25, 17, 9 as buccal, inferior, lingual, between roots. Conclusion : When estimating the mandibular canal and the roots through the panoramic radiography, it could be difficult to drive the views of which this estimation was considerable. Thus it is required to have an accurate diagnostic approaching through CBCT that could estimate the location between mandibular canal and roots.

유기 전기발광소자의 주파수 의존 특성

崔淙宣,李榕洙 弘益大學校 科學技術硏究所 2001 科學技術硏究論文集 Vol.12 No.-

The admittance or impedance spectroscopy is one of the useful tools to study the equivalent circuit model of organic thin film devices and electrical properties of the film, such as dielectric relaxation and dielectric loss. We have investigated the frequency dependent characteristics of indium tin oxide/TPD/aluminum single layer device. The conductance increases with the applied DC bias in the low frequency region. This indicates that the electrical conductivity increases with the applied bias. The capacitance is nearly constant with the bias in the measured frequency region, which indicates the device acts as a parallel plate capacitor. The Cole-Cole plot of the admittances takes a one-semicircle shape, which means that the device can be modeled as a parallel resistor-capacitor equivalent circuit. We have also studied the complex permittivity of the device with the DC bias and frequency. The imaginary part of the permittivity shows the dielectric dispersion at the low frequencies and the dielectric absorption in the high frequency domain. The dispersion is a proportional to a reciprocal of the frequency. The dielectric absorption represents the relaxation peak and the dielectric relaxation time is about 2.65 μs.

단일 처리기간에 의한 들깨의 생육 및 개화조절

최영환,최영훈,강점순,손병구,김용철,최인수,주우홍 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

It's not uncommon for the amount of leaves to be reduced significantly due to the early differentiation of flower-bud by short-day condition when growing perilla for harvesting the leaves in the greenhouse during the winter. This study analyzed the effect of day-length on the growth and regulation of flowering, which are closely related with differentiation of flower and amount of leaves. Development of new leaf was inhibited by short-day treatment for more than 5 days. As the period of short-day was longer, the effect on the development of new leaf inhibition was more evident. Number of leaves was reduced in both 40 days and 110 days short-day treatments as period of short-day length was longer. Short-day treatment for less than 3 days did not induce flowering, but short-day treatment for more than 5 days induced flowering. As the period of short-day treatment was shorter, percentage of flowering was lower and days to flowering was longer. That is, when treated for 20 days, flowering began in 20 days. Seed weight was reduced as the length of short-day treatment was longer.

원자력 사고후 쌀알과 배추내 ^90Sr 및 ^137Cs 농도 예측

최용호,임광묵,황원태,이한수,이창우 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.3

원자력 사고후 시간경과에 따른 작물체내 핵종 농도를 보다 현실적으로 예측할 수 있는 방법을 수립하고 단위 건침적에 대하여 백미와 배추내 ^90Sr 및 ^137Cs의 50 년간 농도를 예측하였다. 비생육기 사고의 경우 농도는 두 핵종 모두 시간경과에 따라 서서히 감소하였고 50년 내내 ^90Sr이 ^137Cs보다 높았다. 생육기 사고의 경우 처음 1년간 농도는 비생육기 사고에 비해 대체로 ^90Sr은 최고 30 배, ^137Cs은 최고 1,000 배 정도까지 높았다. 50 년간의 누적농도는 백미에서는 비 생육기 사고의 경우 ^90Sr이, 생육기 사고의 경우 ^137Cs이 더 높았으나 배추에서는 어느 경우에나 ^90Sr이 더 높았다. 생육기 사고시 50년간의 누적농도에 대한 지배적 경로는 대체로 ^90Sr의 경우 뿌리흡수, ^137Cs의 경우 작물체 직접오염이었다. 재부유의 영향은 무시할 수 있을 정도였다. 예측 결과에 입각하여 사고 조건별로 대책의 방향을 제시하였다. A method of more realistically predicting radionuclide concentrations in crop plants varying with time after a nuclear accident was established to estimate 50 years' concentrations of ^90Sr and ^137Cs in polished rice seeds and Chinese cabbage for unit dry deposition. After non-growing season accidents, concentrations of both nuclides decreased gradually with time and ^90Sr concentrations were higher than those of ^137Cs throughout the whole period. Radionuclide concentrations in the 1st year after growing season accidents were on the whole higher than those after non-growing season accidents by factors of up to 30 for ^90Sr and up to 1,000 for ^137Cs. In polished rice seeds, the 50 years-integrated concentration was higher for ^90Sr than for ^137Cs after non-growing season accidents, whereas the opposite was true after growing season accidents. In Chinese cabbage, however, it was higher for ^90Sr than for ^137Cs after both types of the accident. Generally speaking, the dominant pathway for the integrated concentration after the growing season accident was root uptake for ^90Sr and direct plant contamination for ^137Cs. The effect of resuspension was negligible. Based on the predicted results, the direction of planning countermeasures was suggested for various accident conditions.

화염을 내포하는 영상의 검출에 대한 연구

도용태,최병수 대구대학교 정보통신연구소 2012 情報通信硏究 Vol.9 No.1

화재는 여러 가지 재난들 중에서 가장 흔하다. 따라서 이에 대한 효과적인 대책이 필요하지만, 기존의 연기 검출기를 비롯한 단순한 형태의 센서들은 자주 오작동하는 문제가 있다. 이 문제에 대한 해결책으로 카메라의 영상을 사용하여 화재를 검출하는 방법에 대한 관심이 근래 증대되고 있다. 본 논문에서는 화염을 포함하는 영상을 검출하기 위한 기법이 서술된다. 주어진 영상을 다수의 영역으로 나눈 후, 각 영역의 특성으로부터 화염의 존재 유무를 판정한다. 서술된 기법은 다양한 영상들을 이용하여 그 성능을 평가하였으며, 그 성공률은 약 73%이었다. 이와 같은 결과는 연속 영상에 의한 화재감지에 사용되어 더 높은 성공률로 개선될 수 있을 것이다. detection using camera images. In this paper, a technique to detect images containing flames is described. After segmenting a given image into multiple regions, the existence of flames is determined from the characteristics of each region. The described technique was tested using various images, and we could get about 73% of success rate. This result may be improved by detecting fire in image sequences.

아세클로페낙 연질캡슬(클란자 에스 연질캡슬)의 개발

용철순,이경희,최진석,박병주,정세현,김용일,박상만,배명수,김귀자,김영식,유창훈,강성룡,유봉규,이종달,최한곤 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.1

To develop and aceclofenac soft capsule, four preparations with various solubilizers were prepared and their dissolution test was carried out. Among four preparations tested, a preparation with ethanolamine was selected a formula of aceclofenac soft capsule (Clanza S^(™), since it showed the fastest dissolution rate. Bioequivalence of aceclofenac tablet, Airtal^(™)(Dae-Woong Pharmaceutical Co., Ltd.) and aceclofenac soft capsule, Clanza S^(™)(Korea United Pharmaceutical Co., Ltd.) was evaluated according to the guideline of KFDA. Fourteen normal male volunteers (age 20-25 years old) were divided into two groups and a randomized 2×2 cross-over study was employed. After oral administration of one tablet or capsule containing 100 ㎎ of aceclofenac, blood ws taken at predetermined time intervals and the concentration of aceclofenac in plasma wa determined with an HPLC method under UV detector. The pharmacokinetic parameters (C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameters using logarithmically transformed AUC_(t), C_(max) and T_(max) between Airtal tablet and Clanza soft capsule were 2.89%, 0.18% and 43.0%, respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.250(e.g.log(0.81) - log(1.23) and log(0.89) - log(1.14)) for AUC_(t) and C_(max), respectively. Thus, the criteria of the KDFA guidelines for the equivalence was satisfied, indicating that Clanza S^(™) soft capsule is bioequivalent to Airtal^(™) tablet.

유자종실의 Sterol 조성

최상도,주옥수,조용운 진주산업대학교 농업기술연구소 1993 農業技術硏究所報 Vol.6 No.-

남해산 柚子種實油을 分析試料로 하여 日本, 基準油脂分析試驗法에 따라서 不檢化物量을 定量하고 T. L. C法으로 不檢化物을 分劃하였으며 G. C法에 依해 sterol組成을 分析하였다. 유자종실기름의 불검화물량은 기름 1g당 19.2mg으로서 1.9%이였고 불검화물의 분획 pattern은 less polar compounds가 20.3%, 4,4-dimethylsterol이 9.6%, 4-desmethylsterol이 47.1% 및 미전개물질이 22.9%이였다. sterol조성은 cholesterol이 0.3%, campesterol이 11.9%, stigmasterol이 9.3%, β-sitosterol이 72.2% 및 未知 sterol이 6.3% 이였다. 불검화물량, 불검화물의 분획 pattern 및 sterol조성은 他植物種實油와 거의 같은 경향을 나타냈다. This experiment was performed to the unsaponifiable matters content of the citrus seed oil. Also, the fractionated sterol pattern and the sterol composition of the citrus seed oil were examined. The citrus seed oil were contained 19.2mg unsaponifiable matters per gram. The unsaponifiable matters in seed oil were fractionated into less polar compounds(20.3%), 4, 4-dimethylsterol(9.6%), 4-monomethylsterol(0%), 4-desmethylsterol(47.1%) and undeveloped matters(22.9%). The sterol composition in citrus seed oil were cholesterol 0.3%, campesterol 11.9%, stigmasterol 9.3% and beta-sitosterol 72.2%. But brassicasterol and avenasterol were not detected.