유도 '되치기 본'의 개선 방안에 관한 연구

김의환,김도준,김규수,김관현,김종달,최종삼,조용철,박순진,윤익선,안병근,정 훈,김미정,한성철 龍仁大學校 武道硏究所 1999 武道硏究所誌 Vol.10 No.1

The purpose of this study was to reform practically a Korean Judo's Doechigi-Bon(Forms of Counterattack, judo's Gaeshi no Kata, Judo's Uradori no Kata, Judo's Gonosen no Kata) that was established in 1955 Korea, according to changing of techniques by Judo's modernization, in order to have Judo's carefulness and systematic diffusion. Reform procedure of Judo's Doechigi-Bon was 1st stage, Questionnaire survey 303 judokas, 2nd stage, Technical seminar by judo experts(12 judo professor) 4 times, 3rd stage, wording report for reform, 4rd stage, Discussion and judgement of Teaching and Judgement commission of Korean judo Association(KJA), 5th stage, Public hearing for reform in KJA, 6th stage, Report and decision of board of directors in KJA, 7th stage, public publication of Judo News(No.53) in KJA. Basic principle of reform of Judo's Doechigi-Bon were as table 1. Table 1. Basic principle of reform of Judo's Doechigi-Bon ------------------------------------------------------------------------------- Items Reformed Key Points of Judo's Doechigi-Bon ------------------------------------------------------------------------------- 1. Structure 1) Grand classification : Classified by 3 parts(1,2,3Gyo) 2)Medium classification : Te waza, Goshi waza, Ashi waza devided per each part(Gyo) 3)Sub-classification : Classified five techniques per each part(Gyo) 2. Contents 1) Selected established techniques as possible 2) Considered rationalty and overlapping of counterattack techniques 3. Decision of Conterattack techniques 1) Refered to results of Basic Questionnaire survey 2) Priority to decisions of Judo expert technical seminar -------------------------------------------------------------------------------- Reformed Korean Judo's Doechigi-Bon(Forms of Counterattack techniques-KJA, 1999) are as follows: 1. Gyo; ① Uki otoshi -> Uchi mata ② Harai goshi -> Harai goshi gaeshi ③ O soto gari -> O soto gake ④ Ko uchi gari -> Sasae tsurikomi ashi ⑤ O uchi gari -> Ko soto gari 2. Gyo; ① Ippon seoi nage -> Okuri eri jime ② Tsuri domi goshi -> Uki waza ③ Okuri ashi harai -> Okuri ashi harai ④ Ko soto gari -> Tai otoshi ⑤ Hiza guruma -> Hiza guruma 3. Gyo; ① Kata guruma -> Sumi gaeshi ② Tai otoshi -> Ko soto gari ③ Hane goshi -> Harai tsurikomi ashi ④ Uchi mata -> Tai otoshi ⑤ Tomoe nage -> O uchi gari

항암제 처리한 백혈병 세포주에서의 Apoptosis 발현

김삼용,윤소현,김현수,김종숙,윤환중,김진경,조덕연 충남대학교 암연구소 1998 癌共同硏究所 硏究誌 Vol.2 No.1

The bcl-2 proto-oncogene encodes a 26 kD protein that promotes cell survival by blocking apoptosis. The bax protein is a member of the bcl-2 familly, now known to form heterodimers with the bcl-2 protein. The ratio of bax to bcl-2 is be critical in determining the fate of the cell in response to stimuli that can induce apoptosis. Extract of Pulsatilla Koreana (SB-31) showed promising antitumor activity in vitro with Topo I inhibitory action. In the present study, the relationship between apoptosis and the apoptosis related proteins, bcl-2 and bax were investigated in human leukemic cell lines HL-60, U-937 and CEM-CM3. All anticancer drugs(adriamycin, etoposide, camptothecin, SB-31) induced extensive apoptosis in HL-60, U-937 cells and CEM-CM3 cells. The expression of bcl-2 and bax protein were determined in cell lines by western blotting before and after incubation with anticancer drugs at different time points. 1) In HL-60 or U-937 cell lines, down regulation of bcl-2 and up-regulation of bax were found after incubation with ADR, VP-16 or camptothecin. 2) In HL-60 or U-937 cell lines, no significant change in bcl-2 or bax protein expression resulted after incubation with SB-31. 3) In CEM-CM3 cells, virtually no change was noted in bcl-2/bax expression after incubation with ADR, VP-16, camptothecin or SB-31. It is suggested that different leukemic cell lines use different pathways of apoptosis activation and a given cell may utilize different pathways of apoptosis activation in response to different cytotoxic agents.

이하선관 절단의 임상적 고찰

김한용,김병삼,김환익,이삼용,조백현 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.2

본 병원에 내원한 절단된 이하선관 환자 총 20명(남자:10명 여자 10명)중 4명을 제외한 16명에서 추적조사가 가능하였으며 이 중 14명에서 이하선관의 원활한 관통이 이루어졌고 1명에서 관의 폐세기 생겨 2차 수술이 필료 했으며 다른 1명의 경우 농의 배출이 있었으나 항생제 투여와 구강위생등으로 별 다른 문제 없이 치료 되었고 또 1례의 이하선 타석을 치험하여 보고하는 바이다. Due to increasing trend of traffic accident, number of patient has been gradually increased. Facial injury, for its anatomical vulnerability, is most common site of injury and greatly exceeding injuries of other body region in trauma patient. There are occasional report of Stensen`s duct damage accompanying facial injury and much problem can arise when first aid is performed without knowledge of anatomy or concern for patient. Generally, Stensen`s duct located at mid 1/3 portion of line passing through tragus of ear and subnasale, another word, above the masseter muscle. Once deep lacerated wound has occurred in this region, one must suspect damage of Stensen`s duct. Among the 20 patients (Male: 10, Female:10) who have beeb treated at Chonnam National University Hospital for severed Srensen`s duct, 14 cases have obtained favorable result, not counting 4 patients with whom follow-up study was not possible. In unsuccessful cases of two, 1 case required secondary operation due to occurance of ductal stricture. In another case, leakage of pus has been observed but with proper oral care and antibiotic administration, it has been treated withiut any difficulty. Facial nerve injury accompanying Stensen`s duct been frequently observed, buccal branch being the most common site of involvement. Time interval from severance of duct to repairing the duct was 7 to 21 hours on average and time delay of this level has not impose much limit to obtain successful result. Suture material for Dowel, no matter what kind used, has achieved favorable result and time interval of 9 to 21 days given before the removal was long enough to obtain successful operative result without any development of complication. It is generally accepted view that salivary stone is most common disease entity among the salivery glands and ducts, and stone mostly occur in submandibular duct and rarely in Stensen`s duct. We have performed end-to-end anatomosis using a 23 guage polyethylene catheter as a Dowel in severed duct in 20 patients who have been admitted our hospital for cut repair and obtained satisfiable result. In 1 case, we have experienced stone on the Stensen`s duct, therby reporting this case with through reviews of literature.

냉동 제대혈 세포의 체외 증폭

김삼용,김철희,배광봉,김현수,박상준,김종숙,윤환중,조덕연 충남대학교 암연구소 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Background : Cord blood(CB), which has no HLA restriction, is an alternative to bone marrow for hematopoietic stem cell transplantation. The use of cord blood, however, is limited by the number of progenitor/stem cells necessary to reconstitute the older child or adult. Therefore, ex vivo expansion of CB could have tremendous impact on diverse clinical settings. We studied the ex vivo expansion of isolated population of CD34_(+) cells from cryopreserved CB cells. Methods : CD34 cells were isolated from cryopreserved CB mononuclear cells. Purified cells were cultured with various combinations of hematopoietic growth factors including erythropoietin(EPO), stem cell factor(SCF), granulocyte-colony-stimulating factor(G-CSF), gra-nulocyte, macrophage-colony-stimulating factor(GM-CSF), interleukin-1β(IL-1β), 1L-3, and IL-6. After 7, 10 or 14 days of culture, the fold increases of colony-forming unit- granu-locyte, macrophage(CFU-GM), burst-forming unit-erythroid(BFU-E), colony-forming unit-mix (CFU-Mix), and high proliferative potential colony-forming cell(HPP-CFC) were evaluated. Results : Ten-day culture with the combination of EPO, SCF, G-CSF, IL-1β, and IL-3 resulted in a median of 60-fold increase of CFU-GM, which was greater than those with the combinations of less than 5 growth factors. The addition of IL-6 or GM-CSF to this combination did not enhance CFU-GM expansion. Ten-day culture was significantly superior to 7-day culture for CFU-GM expansion. Prolongation of culture to 14 days, however, revealed decreased expansion of CFU-GM compared to 10 days. BFU-E and CFU-Mix were expanded to 2~5 folds in 7-day culture with the combination of EPO, SCF, and G-CSF. Further expansion was not achieved in 10-day culture and colonies disappeared in 14-day culture. HPP-CFC was expanded to a median of 7.5 folds in 7-day culture with the combination of EPO, SCF, G-CSF, IL-1β, IL-3, and IL-6. Neither 10-day or 14 day-culture enhanced expansion of HPP-CFU. Conclusion : Cryopreserved cord blood cells maintain ex vivo expansion potential. In our system, 10-day culture with the combination consisting of EPO, SCF, G-CSF, IL-1β, and IL-3 seems to be adequate for hematopoietic progenitor/stem cell expansion from cryopreserved cord blood cells.

계층 구조 CC-NUMA 시스템을 위한 브리지 노드 구조

金容三,張星泰 水原大學校 1999 論文集 Vol.17 No.-

The ring provides useful features such as efficient broadcasting and invalidation. However, in hierarchical ring based CC-NUMA system, global ring is the bottleneck of overall system performance. In this paper, we propose a new bridge node architecture for hierarchical expansion of snoop-based CC-NUMA architecture, which can provide filtering and caching effects and then reduces the traffic of global ring and remote access time.

TPA로 야기된 HL-60 세포의 기질부착 저해작용을 이용한 Protein Kinase C 저해 생약의 탐색

김선희,안종석,김삼용,유관희,안병준 충남대학교 약학대학 의약품개발연구소 1993 藥學論文集 Vol.9 No.-

Thirty-five kinds of herbal drugs, believed to be active for treatment of tumors, were selected as the experimental materials for observing their effects on TPA-induced adherence of HL-60 cell and activity of protein kinase C. They were extracted with ethyl ether(E) and ethyl acetate(EA), methanol(M) in sequence. Among the extracts, Sophorae Flos(EA), Paeoniae Radix(EA), Equisetum hiemale(EA), Phellodendri Cortex(E, EA), Mori Cortex radicis(EA), Ferula assafoetida(E, EA), Sophora subprostrata(EA, M) and Cnidium monnieri(E) inhibited the TPA-in-duced adherence of HL-60 cell more than 50% and Sophorae Flos(E, EA, M), Paeoniae Radix(E, EA, M), Eguisetum hiemale(E), Phellodenri Cortex(E,H), Mori Cortex radicis(M), Ferula assafoetida(M), Sophora subprostrata(EA, M), Cnidium monnieri(M) and Artemisia argyi(M) showed inhibiting effects on the activity of protein kinase C more than 50%. The extracts showing good inhibiting effects on the adherence and enzyme activity were Sophorae Flos(EA), Paeoniae Radix(EA), Phellodendri Cortex(E), Sophora subsprostrate(EA) and Equisetum hiemale(EA).

진행위암에 대한 병합화학요법

김삼용,조덕연 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

Twenty-three patients with advanced stage metastatic or unresectable gastric cancer were given combination chemotherapy consisting of Fluorouracil, Doxorubicin, and Cisplatin(FAC) or Mitomycin(FAM). 5 patients receiving FAC and 6 patients receiving FAM were evaluable. 3 patients receiving FAC and 3 patients receiving FAM achieved partial response. So, overal response rate was 60% for FAC and 50% for FAM. Median duration of response was 13 weeks for FAC and 20 weeks for FAM. 1 patient receiving FAC and 2 patients receiving FAM who achieved partial response are alive. Median survival was 8 months for FAC and 7 months for FAM. Responders had a longer survival than nonresponder in both regimens. There was no difference in response and median survival between 2 regimens. Toxicities were mainly gastrointesinal and hematologic, but were moderate and well tolerated in both groups. In conclusion, these regimens have substantial effects on advanced or unresectable gastric cancer without severe toxicities.

성인 급성 백혈병의 치료에 관한 연구

김종완,장준,신승훈,윤일국,박철신,김삼용 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

19 adult ALL patients were treated with follwing regimens; VP regimen to 5 patients, VPA regimen to 4 patients, multi-drug combined intensive therapy of Hoelzer regimen to 10 patients. The results of therapy are as follows: 1. Complete remission occured in 3 patients on VP regimen(60%), 4 patients on VPA regimen (100%), 8 patients on Hoelzer regimen (80%), respectively, and overall complete remission rate was 78.9%. 2. Mean survival time of total patients was 13.8 months, and in complete remission group, it was 16.2 month, but in non-complete remission group, it was 1.8 month. 3. Factors favorable for complete remission were absence of organomegaly(P<0.05) and initial leukocyte count under 30,000/㎟(P<0.01), factors favorable for the survival duration were initial leukocyte count under 30,000/㎟(P<0.05) and remission within 4 weeks of treatment.(P<0.01). 4. Recurrence rate were 40% in VP regimen, 50% in VPA regimen, and 20% in Hoelzer regimen. 5. During the remission induction period, common side effects of chemotherapy were alopecia, nausea, vomiting, mucositis, and general weakness and it was severe in VPA regimen and Hoelzer reimen than in VP regimen. But no significant difference was noted between VPA regimen and Hoelzer regimen. From the above results, we could confirm the importance of multiple drug combined intensive chemotherapy of remission regimen and periodic reinduction chemotherapy.

各種 進行癌에 대한 Cisplatin의 抗癌效果 및 毒性에 關한 硏究

金三溶,金仙英 충남대학교 의과대학 지역사회의학연구소 1983 충남의대잡지 Vol.10 No.2

10 cases of advanced cancer patients were admitted and treated at Chungnam National University Hospital during the period July 1982 till October 1983 with combination chemotherapy containing cisplatin. Among 8 cases of lung caner, 1 each showed complete remission or partial remission and 3 each cases showed stable disease or progressive disease. Their survival ranged 8-42 weeks. 1 case of head and neck cancer and 1 case of endometrial cancer had partial remission respectively, so that among a total of 10 cases 4(40%) showed major response and 3 showed stable disease. A major side effect of cisplatin, nausea and vomiting were effectively prevented by high dose methylprednisolone administration. One case showed decrease of platelet count (61,000/㎣) and tetany during chemotherapy, but otherwise no patient showed elevation of serum creatinine or major toxicities.

대량화학요법을 위한 G-CSF를 이용한 말초조혈모세포의 구동

김삼용,조덕연 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.2

This study probed the effective and economic method for mobilizing the hemopoietic stem cells for the support of high-dose chemotherapy. Eleven trials of Hemopoietic mobilization was performed in 10 patients. A total of 36 leukapheresis was performed(median; 3, range; 2 - 6). Cytotoxic chemotherapy plus G-CSF(300μg/day x 7 days) or G-CSF alone was given to mobilize hemopoietic stem cells. A median of 5.4 x (3.3 - 9.47) x 10 ^8/kg mononuclear cells and 5.1(1.3 - 43) x 10^4/kg GM-CFU was obtained. Nine courses of high-dose chemotherapy was performed in 8 patients. Days with absolute neutrophil count less than 1000/㎣ was 8.5(range ; 3-26) and days with platelet count less than 50.000/㎣ was 14(range; 0-46). Days G-CSF required was 12.5(range ; 9-27) and a median of 100units(range; 10-250) of platelet transfusion was required. Days with antibiotic coverage was 7. 5(0-30) and days of admission was 24(range; 17-51). We can conclude in this study that cyclophosphamide 2g/㎡ and G-CSF 300μg x 7days, or G-CSF for 7 days arc sufficient for the mobilization of hemopoietic stem cells for the high-dose chemotherapy.