流域特性을 考慮한 流出 모델링에 관한 硏究

金治弘,安元植,韓成大,尹如松 成均館大學校 科學技術硏究所 1985 論文集 Vol.36 No.1

This study have been carried out as a sector of the rainfall-runoff relationship research. First of all, the runoff research has the difficult problem due to non-linearity of hydrological phenomena. In this study, according to the method of runoff separation into components by Hino and Hasebe (1980) is applied. This theory considers sufficiently the physical mechanism of runoff process to separate into the groundwater, interflow, and surfaceflow, respectively. Then each component of runoff time series can be reduced to the linear subsystem, that is, ARMA model can be obtained in each subsystem. Actual flood hydrograph of the every two major gauging stations in the Han River basin, the Naktong River basin and the Kum River have been analyzed, and have taken out the characteristics of each river basin. Also the runoff model have obtained for these basins.

만성 활동성 간염에 동반된 간소엽의 변화에 대한 조직학적 연구

李重達,朴耀翰,李昌弘 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.2

The study of liver biopsy specimens taen from 67 Korean adults with B-viral chronic active hepatitis (CAH) revealed lobular inflammation which was seldom seen in European-American patients. These findings suggested that the morphologic difference may be indicative of the ultimate prognosis and pathogenesis for chronic hepatitis B in Korean. The author reviewed biopsy materials form the 67 patients with CAH and found 24 cases of labourlar inflammation of varying degree (35%). Serum aminotransferases (SGOT/SGPT) and hepatitis B viral markers (HBs Ag, Ab-HBs, Ab-HBc, Hbe Ag, Ab-Hbe) were tested at the time of liver biopsy. In view of pathogenesis of the lobular changes in CAH, correlation between histologic changes, SGOT/SGPT, and hepatitis B viral markers was discussed, and the results led to following conclusions. Lobular changes associated with CAH consisted of ballooning degeneration, acidophilic bodies, and spotty necrosis which were present mainly in the centrilobular zone around the terminal venules. No correlation was noted between the degree of lobular inflammation and piecemeal necrosis. Lobular changes in the severe degree, however, correlated to the corresponding degree of piecemeal necrosis. The severe degree of lobular inflammation corresponded with the value of serum aminotransferases. No correlation was, however, found between the mild degree of lobular changes and serum aminotransferase values. Of 20 CAH cases with lobular changes tested for hepatitis B viral markers, 18 wee positive for HBs Ag and 9 Hbe Ag. These findings were indicative of the high infectivity of the CAH with lobular changes.

갑상선 유두암이 병발된 가족성 대장 용종증 1예

백종현,소창배,이은영,이지숙,신은경,박은호,서정아,양재홍,송준영,박선자,박요한,최영식 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Familial adenomatous polyposis (FAP) is an autosomal dominant syndrome, typically characterized by multiple colorectal adenomas and increased incidence of colorectal carcinomas if it is not treated. Moreover, a variety of extracolonic manifestation are seen. The prevalence of thyroid tumors developing in patients with FAP is about 1∼2%. Generally, papillary thyroid cancer indicates low mortality but ,however, as people get older, the cancer becomes more aggressive. So, the operation is required. Recently we experienced a case of FAP, presenting with papillary thyroid carcinoma, and reported with a brief review of literatures.

Anti-obesity effects of Arctii Fructus (Arctium lappa) in white/brown adipocytes and high-fat diet-induced obese mice

Han, Yo-Han,Kee, Ji-Ye,Kim, Dae-Seung,Park, Jinbong,Jeong, Mi-Young,Mun, Jung-Geon,Park, Sung-Joo,Lee, Jong-Hyun,Um, Jae-Young,Hong, Seung-Heon The Royal Society of Chemistry 2016 Food & Function Vol.7 No.12

<P>Arctii Fructus is traditionally used in oriental pharmacies as an anti-inflammatory medicine. Although several studies have shown its anti-inflammatory effects, there have been no reports on its use in obesity related studies. In this study, the anti-obesity effect of Arctii Fructus was investigated in high-fat diet (HFD)-induced obese mice, and the effect was confirmed in white and primary cultured brown adipocytes. Arctii Fructus inhibited weight gain and reduced the mass of white adipose tissue in HFD-induced obese mice. Serum levels of triglyceride and LDL-cholesterol were reduced, and HDL-cholesterol was increased in the Arctii Fructus treated group. In 3T3-L1 cells, a water extract (WAF) and 70% EtOH extract (EtAF) of Arctii Fructus significantly inhibited adipogenesis and suppressed the expression of proliferator-activated receptor gamma and CCAAT/enhancer-binding protein alpha. In particular, EtAF activated the phosphorylation of AMP-activated protein kinase. On the other hand, uncoupling protein 1 and peroxisome proliferator-activated receptor gamma coactivator 1-alpha, known as brown adipocytes specific genes, were increased in primary cultured brown adipocytes by WAF and EtAF. This study shows that Arctii Fructus prevents the development of obesity through the inhibition of white adipocyte differentiation and activation of brown adipocyte differentiation which suggests that Arctii Fructus could be an effective therapeutic for treating or preventing obesity.</P>

Poster Session:PS 1130;Gastroenterology (Liver):A Sarcomatoid Carcinoma Arising from Intrahepatic Cholangiocyte: An Unusual Primary Liver Cancer

( Yo Han Kim ),( Kyoung Hwang Shin ),( Sung Hwahn Hahn ),( Jae Hwan Kong ),( Joon Ho Choi ),( Seoung Min Jeon ),( Hyun Deok Shin ),( Suk Bae Kim ),( Jung Eun Shin ),( Hong Ja Kim ),( Il Han Song ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Sarcomatoid carcinoma arising from intrahepatic cholangiocyte, an extremely rare primary liver cancer, has highly invasive and metastatic potential. The pathogenesis of this tumor is unclear, although histogenetic mechanisms such as ‘transformation’, ‘combination’ and ‘collision’ might be suggested to explain the simultaneous co-existence of carcinoma and sarcoma components in the same tumor. Case: A 58 year-old male presented with a hepatic mass that was found during regular health examination. Liver computed tomography (CT) scan revealed 2. 0 cm-sized heterogeneous low density mass without enhancement located on the segment 6. Magnetic resonance imaging showed the tumor of segment 6 with a low-signal intensity in T1-weighted image and a high-signal intensity in T2-weighted image. Positron emission tomography-CT showed a focal hypermetabolic lesion in the same area, with no evidence of distant metastasis. This mass was suspected to be intrahepatic cholangiocarcinoma by ultrasonography-guided needle biopsy, so right posterior sectionectomy was performed. The resected liver showed a well-defi ned whitish or yellowish gray solid mass with a central hemorrhage and necrosis at a subcapsular area of segment 6. Microscopically, the tumor tissue consists of multiple polygonal and pleomorphic cells. The tumor cells were immunohistochemically positive for cytokeratin 19 and vimentin, but negative for Hep-par 1. The patient was confi rmed a defi nitive diagnosis of intrahepatic sarcomatoid carcinoma. After 2 months, the patient developed extensive abdominal metastases and received systemic chemoradiotherapy, resulting in no response visible radiologically. Conclusions: We report an unusual case of sarcomatoid carcinoma of the liver arising from intrahepatic cholangiocyte, confi rmed by immunohistochemical analysis following surgical resection.

Arctii Fructus Inhibits Colorectal Cancer Cell Proliferation and MMPs Mediated Invasion via AMPK

Han, Yo-Han,Kee, Ji-Ye,Kim, Dae-Seung,Mun, Jeong-Geon,Park, Seong-Hwan,Kim, Yong Ju,Um, Jae-Young,Hong, Seung-Heon Institute for Advanced Research in Asian Science a 2017 The American journal of Chinese medicine Vol. No.

<P>Although Arctii Fructus (AF) has been shown to have various pharmacological effects, there have been no studies concerning the inhibitory effects of AF on the metastatic properties of colorectal cancer (CRC). The aim of this study was to investigate whether AF could suppress CRC progression by inhibiting cell growth, epithelial-mesenchymal transition (EMT), migration, and the invasion ability of CRC cells. AF decreased proliferation of CRC cells by inducing cell cycle arrest and apoptosis via extrinsic and intrinsic apoptotic pathways. Regarding metastatic properties, AF inhibited EMT by increasing the expression of the epithelial marker, E-cadherin, and decreasing the expression of the mesenchymal marker, N-cadherin, in CT26 cells. Moreover, AF decreased the migration and invasion of CT26 cells by inhibiting matrix metalloproteinase-2 (MMP-2) and MMP-9 activity. We confirmed that the decreased invasion ability and MMP-9 activity by AF treatment involved AMP-activated protein kinase (AMPK) activation. Collectively, this study demonstrates that AF inhibits the proliferation and metastatic properties of CRC cells.</P>

Rubrofusarin-6-β-gentiobioside inhibits lipid accumulation and weight gain by regulating AMPK/mTOR signaling

Han, Yo-Han,Kee, Ji-Ye,Park, Seong-Hwan,Mun, Jeong-Geon,Jeon, Hee-Dong,Park, Jinbong,Zou, Qin-Peng,Liu, Xiang-Qian,Hong, Seung-Heon Elsevier 2019 Phytomedicine Vol.62 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Although rubrofusarin-6-β-gentiobioside (RFG), which is a component of <I>Cassiae tora</I> seed, could likely regulate hyperlipidemia, its anti-obesity effect and related mechanism have not been elucidated.</P> <P><B>Purpose</B></P> <P>The aim of this study was to examine whether RFG can ameliorate obesity and the mechanism of lipid accumulation regulated by RFG.</P> <P><B>Study design</B></P> <P>In <I>in vitro</I> experiments, we confirmed the anti-adipogenic effect of RFG using 3T3-L1 cells and human adipose mesenchymal stem cells (hAMSCs). To confirm the anti-obesity effect, High-Fat Diet (HFD)-induced obese mice were selected as a model.</P> <P><B>Methods</B></P> <P>We investigated anti-adipogenic effects of RFG using MTS assay, Oil Red O Staining, real-time RT-PCR, western blot analysis, and immunofluorescence staining. The anti-obesity effect of RFG was confirmed in HFD-induced mice model using hematoxylin and eosin staining and serum analysis.</P> <P><B>Results</B></P> <P>RFG inhibited lipid accumulation in 3T3-L1 cells and hAMSCs by reducing expression of mammalian targets of rapamycin (mTOR), peroxisome proliferator-activated receptor (PPAR)γ, and CCAAT-enhancer binding protein (C/EBP)α. RFG phosphorylated AMP-activated protein kinase (AMPK) in a liver kinase B (LKB) 1-independent manner. Moreover, the anti-adipogenic effect of RFG was blocked by AMPK inhibitor. These results suggest that RFG inhibits lipid accumulation via AMPK signaling. Furthermore, RFG reduced the body weight, size of epididymal white adipose tissue (eWAT), and fatty liver in the mice. RFG also suppressed levels of adipogenic factors PPARγ, C/EBPα, FAS, LPL, and aP2) by activating AMPK in the eWAT and liver.</P> <P><B>Conclusion</B></P> <P>RFG can ameliorate obesity, and thus, could be used as a therapeutic agent for treating obesity.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Phylogenomic analysis of U-Box E3 family in rice

Yo-Han Yoo,Ki-Hong Jung 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

E3 ubiquitin ligase plays a central role in determining specificity of the ubiquitination system by selecting appropriate candidate proteins. Compared with other eukaryotic species there are significantly more U-box protein-encoding genes in plant genome. The rice genome contain 77 U-box-type E3 encoding genes. The 77 members of the rice plant U-box (PUB) E3 family can be placed into 8 major groups based on their domain organizations. In this study, we generate and analyze phylogenomic data integrating anatomical expression patterns consisting of anatomical and stress responsive expression patterns base on 1150 affymetrix arrays to the phylogenic tree of rice plant U-box E3 family. Then, we highlighted 8 genes in subfamilies II (5) and VII (3) of E3 ligases showing significant upregulation in water stress conditions such as drought and high concentration of salt stress. We further developed functional gene network meditated by E3 ligases and refined the components in network by integrating gene expression patterns in response to drought and salt stresses. We expect that our analysis will be a useful platform to facilitate the functional identification for each of U-box E3 family.

An RNA-Seq transcriptome analysis of rice genes in response to water deficiency in soil

Yo-Han Yoo,Anil Kumar N.C,Ki-Hong Jung 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Water-deficiency is one of the most serious challenges which restrict crop production. Root is the primary tissues exposed to water limitation in soil. Although a number of transcriptome data under water limitation have been produced in rice, but most of them have analyzed the effect of leaf or shoot. Thus, understanding of relating molecular mechanism is still limited. To get global view of the effect on water deficiency in rice root, we carried out RNA-Seq experiment. To do this, we compared the RNA-Seq transcriptome data of 3 day samples under water deficiency with those of unstressed rice roots with unstressed control. As a result, we identified 1,098 genes upregulated in water stress condition for 3 days. Gene ontology (GO) enrichment analysis revealed that 18 GO terms are overrepresented. Of them, valyl-tRNA aminoacylation, transcription from RNA polymerase II promoter, glycine catabolic process, and L-phenylalanine catabolic process are more significant, indicating that transcription of new transcripts, control of translation fidelity, and reuse of primary and secondary metabolites can be activated during water stress.

Anti-adipogenic effect of Glycoside St-E2 and Glycoside St-C1 isolated from the leaves of <i>Acanthopanax henryi</i> (Oliv.) Harms in 3T3-L1 cells

Han, Yo-Han,Li, Zhi,Um, Jae-Young,Liu, Xiang Qian,Hong, Seung-Heon Informa UK (TaylorFrancis) 2016 Bioscience, Biotechnology, and Biochemistry Vol.80 No.12

<P>Acanthopanax henryi (Oliv.) Harms has been used in the treatment of arthritis, rheumatism, and abdominal pain. This study evaluated whether natural compounds isolated from the leaves of A. henryi (Oliv.) Harms could inhibit adipocyte differentiation by regulating transcriptional factors such as peroxisome proliferator-activated receptor (PPAR) and CCAAT/enhancer-binding protein (C/EBP). AMP-activated protein kinase (AMPK) activity was also evaluated. Among the several compounds isolated from the leaves of A. henryi (Oliv.) Harms, Glycoside St-C1 and Glycoside St-E2 significantly decreased lipid accumulation and the expressions of PPAR and C/EBP. Glycoside St-C1 and Glycoside St-E2 were found to activate AMPK when they regulated PPAR and C/EBP. Results confirmed that Glycoside St-C1 and Glycoside St-E2 isolated from the leaves of A. henryi (Oliv.) Harms can inhibit adipogenesis through the AMPK-PPAR-C/EBP mechanism. Thus, this study suggests that Glycoside St-C1 and Glycoside St-E2 have a therapeutic effect due to activation of the AMPK.</P>