Vaginal Microbiome Dysbiosis is Associated with the Different Cervical Disease Status

Ma Yingying,Li Yanpeng,Liu Yanmei,Cao Le,Han Xiao,Gao Shujun,Zhang Chiyu 한국미생물학회 2023 The journal of microbiology Vol.61 No.4

Vaginal microbiome composition was demonstrated to be associated with cervical disease. The colonization characteristics of vaginal microbes and their association with the different cervical disease status, especially cervical cancer (CC), are rarely investigated. In this cross-sectional study, we characterized the vaginal microbiome of women with different status of cervical diseases, including 22 NV + (normal tissue with HPV infection), low-grade squamous intraepithelial lesion (LSIL, n = 45), high-grade squamous intraepithelial lesion (HSIL, n = 36) and CC (n = 27) using bacterial 16S DNA sequencing. Thirty HPV-negative women with normal tissue were used as the control group. We found that higher diversity of microbiome with gradual depletion of Lactobacillus, especially L. crispatus, was associated with the severity of cervical disease. High-risk HPV16 infection was associated with higher microbiome diversity and depletion of Lactobacillus in high-grade cervical diseases (i.e. HSIL and CC). The CC group was characterized by higher levels of Fannyhessea vaginae, Prevotella, Bacteroides, Finegoldia, Vibrio, Veillonella, Peptostreptococcus, and Dialister. Co-occurrence network analyses showed that negative correlations were exclusively observed between Lactobacillus and other bacteria, and almost all non-Lactobacillus bacteria were positively correlated with each other. In particular, the most diverse and complex co-occurrence network of vaginal bacteria, as well as a complete loss of L. crispatus, was observed in women with CC. Logistic regression model identified HPV16 and Lactobacillus as significant risk and protective factors for CC, respectively. These results suggest that specific Lactobacillus species (e.g. L. crispatus and L. iners) can be used as important markers to target prevention measures prioritizing HPV16-infected women and other hrHPV-infected women for test, vaccination and treat initiatives.

To Bid or Not to Bid? - Keyword Selection in Paid Search Advertising

Ma, Yingying,Sun, Luping Korean Marketing Association 2014 ASIA MARKETING JOURNAL Vol.16 No.3

The selection of keywords for bidding is a critical component of paid search advertising. When the number of possible keywords is enormous, it becomes difficult to choose the best keywords for advertising and then subsequently to assess their effect. To this end, we propose an ultrahigh dimensional keyword selection approach that not only reduces the dimension for selections, but also generates the top listed keywords for profits. An empirical analysis using a unique panel dataset from a large online clothes retailer that advertises on the largest search engine in China (i.e., Baidu) is presented to illustrate the usefulness of our approach.

Value of Children among Chinese Mothers: Cohort Effects and Urban-rural Differences*

Ma LI,Yingying JIANG,Liqi ZHU 한국공공가치학회 2021 공공가치연구 Vol.1 No.1

China is facing a dramatic decrease of fertility rate, even after the one-child policy has been changed to two-children policy. Psychologists propose that value of children may account for willingness to deliver babies. Value of children (VOC) includes VOC+ (which means the positive values of children) and VOC- (the cost and expenditure of having children). This study compared value of children among different cohorts of Chinese women and women in urban and rural areas in China. In Study 1, 203 mothers of childbearing age in China were recruited to complete VOC scale. Their responses were then compared with 208 mothers born in 1920~1949 and 206 mothers born in 1950~1979 in the study by Zheng et al., (2005). Cohort effects were found in all VOC dimensions, the current generation pays less attention to children s traditional value of economic support and old-age security, and pays more attention to children s emotional and familial values, as well as the disadvantages and constraints in delivering babies, indicating Chinese women s values of children have changed over time. In Study 2, women in three areas of different economic levels were recruited. Results showed differences in both VOC+ and VOC- dimensions: women in remote rural areas put more emphasis on “traditional VOC”, while participants in urban areas rated higher in VOC-. This may explain why the fertility rate in urban area is even lower than that in rural area in China. Reasons of intergenerational and urban-rural differences are further discussed.

习近平“两个结合”重要论断的逻辑取向与实践方向

宋颖颖(Yingying Song),马婷婷(Tingting Ma) YIXIN 출판사 2023 Journal of China Studies Vol.1 No.1

“两个结合”是习近平总书记对新时代继续推进马克思主义中国化所做出的重要指示,为中国科学社会主义理论未来发展方向指明了前进道路,探究“两个结合”的逻辑取向与实践方向有利于助力其顺利推进。推进“两个结合”实则是应然、使然、必然的逻辑建构,是对马克思主义理论的贯彻、对历史经验的遵守、对新时代伟大实践的认同。从实践方向看,推进“两个结合” 必须坚决守住科学社会主义底色、坚定保持当代中国社会本色、坚持发掘中华优秀文化特色,从而为马克思主义中国化贡献源源不断的崭新助推力。 “Two Combinations” is an important instruction given by General Secretary Xi Jinping to continue to promote the Sinicization of Marxism in the new era. It points out the way forward for the future development direction of China’s scientific socialist theory. Exploring the logical orientation and the practical direction of the “Two Combinations” is conducive to helping it to advance smoothly. Promoting the “two combinations” is actually a logical construction that should be and is necessary. It is the implementation of Marxist theory, the observance of historical experience, and the recognition of the great practice of the new era. From a practical perspective, to promote the “Two Combinations”, we must resolutely maintain the foundation of scientific socialism, resolutely maintain the true nature of contemporary Chinese society, and persist in exploring the outstanding cultural characteristics of China, thereby contributing a steady stream of new impetus to the Sinicization of Marxism.

Development of manganese ferrite/graphene oxide nanocomposites for magnetorheological fluid with enhanced sedimentation stability

Guangshuo Wang,Yingying Ma,Yu Tong,Xufeng Dong 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.48 No.-

Novel nanocomposites consisting of manganese ferrite nanoparticles and graphene oxide nanosheets(MnFe2O4/GO) have been synthesized as a promising candidate for magnetorheological (MR)fluid. Themorphology, microstructure, composition and magnetic properties of the obtained MnFe2O4/GO werestudied in detail. It was found that the MnFe2O4 nanoparticles with diameter of 8–12 nm were denselydecorated on the surface of GO nanosheets. The magnetization investigation revealed that as-preparedMnFe2O4/GO had superparamagnetic behavior with saturation magnetization of 36.2 emu/g. The MRfluid was prepared by the obtained MnFe2O4/GO and the corresponding MR properties were investigatedusing a Physica MCR301 rheometerfitted with a magneto-rheological module. The MnFe2O4/GO-basedMRfluid exhibited typical MR effect with increasing shear stress, yield stress and dynamic shear modulusdepending on magneticfields. More importantly, the sedimentation stability of the prepared MRfluidwas found to be improved due to the unique sheet-like structure and the reduced density mismatchbetween the dispersed particles and the carrier medium. The MnFe2O4/GO-basedfluid with typical MReffect and excellent sedimentation stability would provide a feasible candidate for practical applications

Controlled synthesis of CoFe2O4/MoS2 nanocomposites with excellent sedimentation stability for magnetorheological fluid

Guangshuo Wang,Fei Zhou,Ziwei Lu,Yingying Ma,Xiaoguang Li,Yu Tong,Xufeng Dong 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.70 No.-

In this study, we reported novel nanocomposites containing cobalt ferrite (CoFe2O4) nanoparticles andmolybdenum disulfide (MoS2) nanosheets (CoFe2O4/MoS2). The obtained CoFe2O4/MoS2 as dispersedphase was used to prepare magnetorheological (MR)fluid and the rheological properties wereinvestigated in detail. The results of transmission electron microscopy showed that the CoFe2O4nanoparticles were homogeneoused decorated on the surface of MoS2 nanosheets. The successfulpreparation of CoFe2O4/MoS2 was further confirmed by X-ray diffraction, X-ray photoelectronspectroscopy, Raman spectroscopy and vibrating sample magnetometer. The CoFe2O4/MoS2 based MRfluid exhibited typical MR effect with increasing viscosity, shear stress, yield stress and dynamic shearmoduli depending on external magneticfields. More importantly, the preparedfluid showed excellentsedimentation stability due to unique two-dimensional structure and reducedfluid-particle densitymismatch.

Polydopamine Used as Hollow Capsule and Core-Shell Structures for Multiple Applications

Xinyu Cui,Yuanyuan Yin,Zuo Ma,Yongkui Yin,Yue Guan,Shengzhong Rong,Jinxia Gao,Yingying Niu,Miaojing Li 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.5

Polydopamine (PDA) capsule and core – shell structures with tailored structures and properties are of particular interests due to their multifunctions and potential applications as new colloidal structures in diverse fields. Among the available fabrication methods, PDA film onto colloidal particles followed by selective template removal has attracted extensive attention due to its advantages of precise control over the size, wall thickness and functions of the obtained capsules. The past several years has witnessed a rapid increase of research concerning the new fabrication strategies, functionalization and applications of this kind of capsules and core – shell structures, particularly in many fields such as drug delivery, catalysis, antibacterial, etc. In this review, the very recent progress of the capsule and core – shell structures based on PDA are summarized. There are basically two sections, including the fabrication process of PDA capsules, core – shell structures, and the various applications based on PDA.

Reference Intervals for Thyroid-Associated Hormones and the Prevalence of Thyroid Diseases in the Chinese Population

Zou Yutong,Wang Danchen,Cheng Xinqi,Ma Chaochao,Lin Songbai,Hu Yingying,Yu Songlin,Xia Liangyu,Li Honglei,Yin Yicong,Liu Huaicheng,Zhang Dianxi,Zhang Kui,Lian Xiaolan,Xu Tengda,Qiu Ling 대한진단검사의학회 2021 Annals of Laboratory Medicine Vol.41 No.1

Background: Thyroid diseases are highly prevalent worldwide, but their diagnosis remains a challenge. We established reference intervals (RIs) for thyroid-associated hormones and evaluated the prevalence of thyroid diseases in China. Methods: After excluding outliers based on the results of ultrasound screening, thyroid antibody tests, and the Tukey method, the medical records of 20,303 euthyroid adults, who visited the Department of Health Care at Peking Union Medical College Hospital from January 2014 to December 2018, were analyzed. Thyroid-associated hormones were measured by the Siemens Advia Centaur XP analyzer. The RIs for thyroid-associated hormones were calculated according to the CLSI C28-A3 guidelines, and were compared with the RIs provided by Siemens. The prevalence of thyroid diseases over the five years was evaluated and compared using the chi-square test. Results: The RIs for thyroid stimulating hormone (TSH), free thyroxine (FT4), free triiodothyronine (FT3), total thyroxine (TT4), and total triiodothyronine (TT3) were 0.71–4.92 mIU/L, 12.2–20.1 pmol/L, 3.9–6.0 pmol/L, 65.6–135.1 nmol/L, and 1.2–2.2 nmol/L, respectively. The RIs of all hormones except TT4 differed significantly between males and females. The RIs of TSH increased with increasing age. The prevalence of overt hypothyroidism, overt hyperthyroidism, subclinical hypothyroidism, and subclinical hyperthyroidism was 0.5% and 0.8%, 0.2% and 0.6%, 3.8% and 6.1%, and 3.3% and 4.7% in males and females, respectively, which differed from those provided by Siemens. Conclusions: Sex-specific RIs were established for thyroid-associated hormones, and the prevalence of thyroid diseases was determined in the Chinese population.

Comparison of Direct and Extraction Immunoassay Methods With Liquid Chromatography-Tandem Mass Spectrometry Measurement of Urinary Free Cortisol for the Diagnosis of Cushing’s Syndrome

Mu Danni,Fang Jiadan,Yu Songlin,Ma Yichen,Cheng Jin,Hu Yingying,Song Ailing,Zhao Fang,Zhang Qi,Qi Zhihong,Zhang Kui,Xia Liangyu,Qiu Ling,Zhu Huijuan,Cheng Xinqi 대한진단검사의학회 2024 Annals of Laboratory Medicine Vol.44 No.1

Background: Twenty-four-hour urinary free cortisol (UFC) measurement is the initial diagnostic test for Cushing’s syndrome (CS). We compared UFC determination by both direct and extraction immunoassays using Abbott Architect, Siemens Atellica Solution, and Beckman DxI800 with liquid chromatography-tandem mass spectrometry (LC-MS/MS). In addition, we evaluated the value of 24-hr UFC measured by six methods for diagnosing CS. Methods: Residual 24-hr urine samples of 94 CS and 246 non-CS patients were collected. A laboratory-developed LC-MS/MS method was used as reference. UFC was measured by direct assays (D) using Abbott, Siemens, and Beckman platforms and by extraction assays (E) using Siemens and Beckman platforms. Method was compared using Passing–Bablok regression and Bland–Altman plot analyses. Cut-off values for the six assays and corresponding sensitivities and specificities were calculated by ROC analysis. Results: Abbott-D, Beckman-E, Siemens-E, and Siemens-D showed strong correlations with LC-MS/MS (Spearman coefficient r=0.965, 0.922, 0.922, and 0.897, respectively), while Beckman-D showed weaker correlation (r=0.755). All immunoassays showed proportionally positive bias. The areas under the curve were 0.975 for Abbott-D, 0.972 for LC-MS/MS, 0.966 for Siemens-E, 0.948 for Siemens-D, 0.955 for Beckman-E, and 0.877 for Beckman-D. The cut-off values varied significantly (154.8–1,321.5 nmol/24 hrs). Assay sensitivity and specificity ranged from 76.1% to 93.2% and from 93.0% to 97.1%, respectively. Conclusions: Commercially available immunoassays for measuring UFC show different levels of analytical consistency compared to LC-MS/MS. Abbott-D, Siemens-E, and Beckman-E have high diagnostic accuracy for CS.

Facile synthesis of hierarchically structured flower-like Fe3O4 microspheres for high-performance magnetorheological fluids

Guangshuo Wang,Dexing Zhao,Nannan Li,Yingzhe Zeng,Shuai Han,Yingying Ma,Xufeng Dong,Ruitao Yu 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.79 No.-

In this study,flower-like Fe3O4 microspheres with hierarchical structure were prepared by a one-stepsolvothermal method, and the obtainedflower-like Fe3O4 microspheres were adopted as a newmagnetorheological (MR) material to prepare a uniform MR suspension with high performances. Thesynthesized samples were systematically examined using FE-SEM, TEM, TGA, XRD, XPS, BET and VSM. The preparedflower-like Fe3O4 microspheres-based MRfluid indicated typical MR properties, asevidenced by the fact that shear stress, yield stress, viscoelastic storage modulus and loss modulus of MRfluid strongly depended on magneticfield strengths. Specifically, the resultant MRfluid showed goodsedimentation stability with sedimentation ratio of 86.5% in the settling period. The excellentsedimentation stability could be attributed to the uniqueflower-like hierarchical structure, high specificsurface area and the reduced particle-fluid density mismatch.