Co-aggregation of Enzyme and Amyloid Fibrils

Seul Gi KIM,Song Yi BAE,T. Doohun Kim 한국생물공학회 2011 한국생물공학회 학술대회 Vol.2011 No.10

Characerization of Cross-Linked Enzyme Aggregates of Acyl-CoA thioesterase1 precursor from Listeria innocus CLIP 11262

Seul Gi KIM,Song Yi BAE,Nam Yong KIM,So Hyun PARK,T. Doohun KIM 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

Amyloid formation using 1-butyl-3-methylimidazolium[BMIM]-based ionic liquids

Song Yi BAE,Seul-gi KIM,T.Doohun KIM 한국생물공학회 2011 한국생물공학회 학술대회 Vol.2011 No.10

Charaterization of a novel SGNH Hydrolase from Burkhoderia glumae

Song Yi BAE,Seul-Gi KIM,Nam Yong KIM,So Hyun PARK,T. Doohun KIM 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

흑대추와 일반 건조대추의 추출 및 유산발효과정 중 특성 변화

어미선(Mi Sun Auh),김이슬(Yi Seul Kim),안승준(Seung Joon Ahn),안준배(Jun Bae Ahn),김광엽(Kwang Yup Kim) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.10

This study was carried out to investigate the characteristics of black jujube and Zizyphus jujube extracts during lactic acid fermentation. Both extracts were fermented using Lactobacillus fermentum YL-3. As a result, viable cell number rapidly increased until 24 hours, after which it gradually decreased. Before lactic acid fermentation, the IC50 of black jujube, which was 0.014 mg/mL, was lower than that of Zizyphus jujube. Further, black jujube showed stronger antioxidant activity (374.21 mg AA eq/g) than Zizyphus jujube. Contents of total polyphenolics in both extracts were 15.46 mg/g and 13.61 mg/g, respectively, whereas contents of total flavonoids were 374.21 μg/g and 64.25 μg/g. After lactic acid fermentation, there was no significant increase in DPPH or ABTS free radical scavenging activity. Total polyphenolic content of Zizyphus jujube decreased to 12.39 mg/g upon fermentation, whereas flavonoid content significantly increased to 291.58 μg/g. Further, polyphenolic and flavonoid contents of black jujube increased from 15.46 mg/g to 17.46 mg/g and from 374.21 μg/g to 1,135.29 μg/g, respectively. These results demonstrate that 9-Times Steamed and Dried increased functional components. Especially, lactic acid fermented black jujube showed remarkably high antioxidant activity. These results confirm the potential use of lactic acid fermented black jujube as a valuable resource for the development of functional foods.

진로정체감과 Krumboltz의 과제접근기술이 진로성숙도, 진로장벽, 진로포부에 미치는 영향

김경미(Kyoung-Mi Kim),배영광(Young-Kwang Bae),민이슬(Yi-Seul Min),장성화(Sung-Hwa Jang) 한국콘텐츠학회 2014 한국콘텐츠학회논문지 Vol.14 No.11

본 연구는 진로정체감과 Krumboltz의 과제접근기술 중 낙관성, 위험감수, 불확실한 상황에 대한 인내가 진로성숙도, 진로장벽, 진로포부에 미치는 영향에 대해서 살펴보았다. 연구대상은 서울, 경기, 그리고 충청지역 대학에 재학 중인 대학생 269명을 대상으로 진로정체감, 과제접근기술, 진로성숙도, 진로장벽, 진로포부 검사도구를 사용하여 상관분석과 중다회귀분석을 실시하였다. 연구결과에 따르면 첫째, 상관분석 결과 진로정체감은 진로성숙 진로포부와 정적상관을, 진로장벽과는 부적상관을 보였으며, 낙관성은 진로장벽과 부적상관을 보였다. 또한 불확실한 상황에 대한 인내는 진로성숙과는 정적상관을, 진로장벽과는 부적상관을 보였다. 둘째, 진로정체감과 과제접근기술이 진로성숙, 진로장벽, 진로포부에 미치는 영향을 살펴 본 결과, 진로성숙에는 진로정체감, 낙관성, 불확실한 상황에 대한 인내부족이 영향을 미치는 것으로 나타났으며, 진로장벽에는 진로정체감, 낙관성, 불확실한 상황에 대한 인내가 영향을 미치는 것으로 나타났다. 한편 진로포부에는 낙관성만이 영향을 미치는 것으로 나타났다. 이러한 결과는 진로상담에서 과제접근기술이 진로에 대한 태도, 진로장벽, 진로포부에 미치는 영향을 인식하고, 내담자들이 낙관성과 불확실한 상황에 대한 인내를 개발할 수 있도록 조력해야 함을 보여주었다. 이밖에도 본 연구에서는 연구결과의 의미와 제한점에 대해서 논의하였다. The current study examined the relationships between task-approach skills of optimism, bearability, intolerance of uncertainty, career identity, career maturity, career barriers, and career aspiration. Data was collected from two-hundred sixty nine college students who matriculated in colleges located in Seoul, Gyeoungi and Chungchung provinces in Korea. The correlation analysis and multiple linear regression were carried out to explore the relationship among the variables mentioned above. The results of this study were as follows: First, career identity was positively correlated with career maturity, aspiration and negatively with career barrier. optimism was negatively correlated with career barrier. in addition, intolerance of uncertanity was positively correlated with career maturity. and negatively with career barriers. Second, multiple linear regression revealed that career identity, optimism and lacking intolerance of uncertanity significantly influenced career maturity, career identity, optimism and intolerance of uncertanity significantly influnced career barriers. In addition, only optimism influnced career aspiration. These findings implied that career counselors need to assist clients developing optimism, flexibility, bearability and counselors should be aware of the effect of task-approach skills on career, barriers, and aspiration. Further discussions regarding results are included.

대추 추출물이 장내 미생물의 생육에 미치는 영향 및 항산화 활성

정혜미(Hye Mi Jeong),김이슬(Yi Seul Kim),안승준(Seung Joon Ahn),어미선(Mi Sun Auh),안준배(Jun Bae Ahn),김광엽(Kwang Yup Kim) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.4

본 연구에서는 대추 추출물과 장내 유익균의 생육을 조절하여 장내 부패를 방지하기 위한 probiotics로서 유산균을 조합하기 위하여 대추 추출물이 장내 유익균 중에서도 유산균의 생육에 미치는 영향을 in vitro 상에서 알아보고자 하였다. 또한 증식인자로서 대추 추출물에 존재하는 당이 유산균의 생육에 어떠한 영향을 미치는가를 알아보기 위하여 추출방법을 달리하여 당 함량이 다른 대추 1차 및 2차 열수, 에탄올 추출물을 각각 제조하였다. 이와 더불어 지역 특산물인 보은대추를 기능성식품 소재로 활용하기 위하여 페놀성 화합물과 항산화성을 검색하여 기능성식품으로 상품화하기 위한 기본 자료를 확보하고자 하였다. TLC와 HPLC 분석을 통해 장내 유익균의 증식인자로 작용할 수 있는 대추 추출물의 당 조성은 fructose, glucose, sucrose인 것을 확인하였고, 1차 추출물에 비하여 2차 열수 추출물은 fructose, glucose, sucrose가 각각 69, 74, 89% 감소하였으며 2차 에탄올 추출물은 63, 62, 83% 감소한 것을 확인할 수 있었다. 인체 장내세균에 대하여 대추 추출물을 50 mg/mL 농도로 하여 생육증진 효과를 분석한 결과, 대추 1차 열수 추출물에서 B. bifidum과 B. adolescentis가 다른 시험균주들에 비하여 대조군과 대수기에 도달하는 시간은 비슷하였지만, 대조군보다 높은 흡광도 값을 나타내어 생육을 증진시키는 결과를 보였다. 이는 대추 추출물에 존재하는 당이 prebiotics로 작용하여 Bifidobacterium 속의 생육을 증진시킨 것으로 사료되며 인체 장내 균총 조성을 바람직한 방향으로 개선시켜 장내 기능을 향상시킬 것으로 기대된다. 또한 당이 아닌 다른 기능성 성분에 의해 생육이 증진되는지를 알아보기 위해 기능성 물질인 페놀류의 함량과 항산화성을 분석하였다. 총 폴리페놀과 총 플라보노이드 함량을 측정한 결과, 대추 1차 열수 추출물에서 각각 11.63 mg/g, 247.45 μg/g으로 다른 추출물에 비해 높은 함량을 보여 주었다. DPPH에 의한 라디칼 소거능을 살펴본 결과, 대추 추출물의 농도가 높을수록 우수한 전자공여능을 나타내었으며 대추 1차 열수 추출물의 경우 다른 추출물에 비해 가장 높은 85.60%의 전자공여능을 보여 천연 항산화제인 ascorbic acid에 견줄 수 있는 기능성이 있음을 확인하였다. ABTS free 라디칼 소거활성을 통해서도 대추 1차 열수 추출물은 679.91 mg AA eq/g의 항산화력을 지니고 있어 다른 추출물에 비해 가장 좋은 항산화 활성을 나타내었다. 따라서 대추 1차 열수 추출물에는 당뿐만 아니라 항산화능, 폴리페놀, 플라보노이드 함량도 높아 B. bifidum과 B. adolescentis의 생육이 증진된 것으로 사료되며, 기능성 식품소재로 생리활성 성분을 함유하고 있는 대추추출물에 Bifidobacterium 속을 이용하여 유산발효를 진행함으로써 기능성 발효 음료 개발 및 이유식과 같은 다양한 식품 개발에 응용될 수 있을 것으로 판단된다. This study was carried out to investigate the effects of jujube extracts on intestinal microflora, along with their antioxidant activities, according to extraction method. The antimicrobial activities of the extracts were measured using the agar diffusion method with a jujube extract concentration of 50 mg/mL. Neither the first nor second jujube extracts were inhibitory against the tested intestinal bacteria. However, water extracts of jujube significantly enhanced the growth of lactic acid bacteria, especially Bifidobacterium bifidum and Bifidobacterium adolescentis. Total phenol compounds and flavonoid compounds were higher in the 1st than in the 2nd water extracts. The EDA values of both water and ethanol extracts increased in proportion to the extract concentration. The 1st water extract showed the highest value among all the others, which was 85.60% at the concentration of 0.05 mg/mL. Furthermore, the 1st water extract showed stronger antioxidant activity than the other samples with an activity of 679.91 mg AA eq/g. These results support the potential use of jujube water extracts as a functional food component and a valuable resource for the development of nutraceutical foods, to increase the growth of Bifidobacterium spp. in the human intestine.

Comparison of anti-influenza virus activity and pharmacokinetics of oseltamivir free base and oseltamivir phosphate

Shin, Jin Soo,Ku, Keun Bon,Jang, Yejin,Yoon, Yi-Seul,Shin, Daeho,Kwon, Oh Seung,Go, Yun Young,Kim, Seong Soon,Bae, Myoung Ae,Kim, Meehyein MICROBIOLOGICAL SOCIETY OF KOREA 2017 JOURNAL OF MICROBIOLOGY -SEOUL- Vol. No.

<P>Influenza viruses are major human respiratory pathogens that cause high morbidity and mortality worldwide. Currently, prophylactic vaccines and therapeutic antiviral agents are used to prevent and control influenza virus infection. Oseltamivir free base (OSV-FB), a modified generic antiviral drug of Tamiflu (oseltamivir phosphate, OSV-P), was launched in the Republic of Korea last year. Here, we examine the bioequivalence of these two compounds by assessing their antiviral efficacy in infected cells and in a mouse model. It was observed that both antivirals showed comparable efficacy against 11 different influenza A and B viruses in vitro. Moreover, in mice infected with influenza A virus (mouse-adapted A/Puerto Rico/8/34), they showed a dose-dependent therapeutic activity and alleviated infection-mediated reductions in body weight, leading to significantly better survival. There was histopathological disappearance of virus-induced inflammatory cell infiltration of the lung after oral treatment with either antiviral agent (at 10 mg/kg). Pharmacokinetic analysis also exhibited similar plasma concentrations of the active drug, oseltamivir carboxylate, metabolised from both OSV-B and OSV-P. This is the first report showing bioequivalence of OSV-FB to its phosphate salt form in the mouse system. The free base drug has some beneficial points including simple drug formulation process and reduced risk of undesirable cation-phosphate precipitation within solution. The long term stability of OSV-FB requires further monitoring when it is provided as a national stock in readiness for an influenza pandemic.</P>

Zebrafish notch signaling is necessary for differentiation of neural stem cell in trigeminal ganglia

Hyung-Soo Kim,Subin Jo,Yunsang Kang,Eun-Bin Kim,Yi-Seul Bae,Su-Jin Kim,Sang-In Park,Chang-Kyu Yoon,Jae-Ho Ryu,Hee Jeong Kong,Sang-Yeob Yeo 한국수산과학회 양식분과 2021 한국수산과학회 양식분과 학술대회 Vol.2021 No.4

<i>Rubus idaeus</i> L. (red raspberry) blocks UVB-induced MMP production and promotes type I procollagen synthesis via inhibition of MAPK/AP-1, NF-κβ and stimulation of TGF-β/Smad, Nrf2 in normal human dermal fibroblasts

Gao, Wei,Wang, Yu-shuai,Hwang, Eunson,Lin, Pei,Bae, Jahyun,Seo, Seul A.,Yan, Zhengfei,Yi, Tae-Hoo Elsevier 2018 Journal of photochemistry and photobiology Biology Vol.185 No.-

<P><B>Abstract</B></P> <P>Chronic exposure to ultraviolet (UV) radiation causes photo-oxidation, which in turn results in the upregulation of matrix metalloproteinases (MMPs) and loss of collagen. <I>Rubus idaeus</I> L. (RI), also called red raspberry, is an important cash crop that contains abundant antioxidant compounds. Sanguiin H-6 and lambertianin C are the major ingredients presented in the extracts. Here, we studied the protective effect of RI on UVB-induced photoaging in normal human dermal fibroblasts (NHDFs). We found that RI notably reduced UVB-induced MMPs secretion and pro-inflammatory mediators production, and significantly suppressed UVB-induced activation of mitogen-activated protein kinase (MAPK), nuclear factor-κβ, as well as activator protein 1. Additionally, treatment of NHDFs with the ERK inhibitor (PD98059) and JNK inhibitor (SP600125) resulted in the reduction of UVB-induced MMP-1 and IL-6 expressions, which demonstrated that the inhibition of MMP-1 and IL-6 by RI is associated with the MAPK pathway. Furthermore, we also found that RI accelerated procollagen type I synthesis by activating the transforming growth factor-β/Smad pathway and enhanced the expression of cytoprotective antioxidants such as heme oxygenase-1 and NHD(P)H quinone oxidoreductase 1 by promoting nuclear factor E2-related factor 2 nuclear transfer. Overall, these findings demonstrated that RI was potentially effective in preventing UVB induced skin photoaging.</P> <P><B>Highlights</B></P> <P> <UL> <LI> RI blocks MMPs and IL-6 secretion by inhibition of MAPK/AP-1 signaling pathway. </LI> <LI> RI promotes procollagen type I synthesis by activation of TGF-β/Smad signaling pathway. </LI> <LI> RI enhances cytoprotective antioxidants expression by stimulation of Nrf2 nuclear transfer. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Chronic exposure to UV radiation results in skin premature aging (photoaging), clinically accompanied with loss of elasticity and tension. In this study, we demonstrated that RI could reverse this damage via increasing procollagen synthesis and blocking MMPs, inflammatory factors expression. The studies of molecular mechanism indicated RI could inhibit MMPs expression via inactivating MAPK/AP-1, NF-κβ pathway and accelerate type I procollagen synthesis through activating the TGF-β/Smad pathway. Furthermore, RI reduced UVB-enhanced ROS via promoting Nrf2 nuclear location and enhancing the expression of cytoprotective antioxidants.</P> <P>[DISPLAY OMISSION]</P>