Application of hyperspectral imaging to identify haploid and diaploid maize seeds

Yeongtae Kim,Minji Kim,Younguk Kim,Jaeyoung Kim,Nyunhee Kim,Eunsook An,JeongHo Baek,Hwang Weon Jeong,Hyenso Ji,Jaeil Ryu,Seung Hyun Wang,Si Hwan Ryu,Song Lim Kim 한국육종학회 2023 한국육종학회 공동학술발표집 Vol.2023 No.-

Identification of a Cys-motif Gene, TSP13, as a Putative Host Translation Inhibitory Factor in Plutella xylostella-Cotesia plutellae

Eunseong Kim,Yeongtae Kim,Yonggyun Kim 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.04

Translational control is a strategy for various viruses to manipulate their hosts to suppress any acute antiviral activity. Some cys-motif genes encoded in polydnaviruses or teratocytes act as host translation inhibitory factor (HTIF) to defend the host antiviral activity. A novel cys-motif gene, TSP13, was encoded in the genome of an endoparasitoid wasp, Cotesia plutellae. TSP13 consists of 129 amino acid residues with a predicted molecular weight of 13.987 kDa and pI value at 7.928. Genomic DNA region encoding open reading frame is interrupted with three introns. TSP13 was expressed in Plutella xylostella larvae parasitized by C. plutellae. C. plutellae bracovirus (CpBV) was purified and injected to nonparasitized P. xylostella. In the virus-injected P. xylostella, TSP13 was shown to be expressed by RT-PCR analysis. Thus, TSP13 was turned out to be encoded in the proviral CpBV genome. TSP13 was cloned into a eukaryotic expression vector, which was then used to infect Sf9 cells to transiently express TSP13. The synthesized TSP13 was detected in the culture broth. Purified TSP13 significantly inhibited cellular immune responses. Furthermore, TSP13 entered the target cells and was localized in the cytosol. This study reports a novel cys-motif gene, which is encoded in CpBV genome localized on chromosome(s) of C. plutellae and replicated to be encapsidated in the episomal viral particles during parasitization.

형광영상을 이용한 옥수수 반수체 종자 선발 효율성 향상

김영욱(Younguk Kim),한정헌(Jeong Heon Han),김재영(Jaeyoung Kim),김영태(Yeongtae Kim),김년희(Nyunhee Kim),이채원(Chaewon Lee),이서연(Seoyeoun Lee),김송림(Song Lim Kim),김문종(Moon Jong Kim),류시환(Si Hwan Ryu),이홍로(Hongro Lee),지현소(H 한국육종학회 2022 한국육종학회지 Vol.54 No.4

Many studies concerning breeding maize varieties are in progress in Korea and other countries. Double haploid technology is widely used for the development of commercial maize varieties worldwide, and has also been utilized in Korea since its introduction by the Maize Research Institute, Gangwondo. We performed a study to improve the efficiency of selecting haploid maize seeds using fluorescence imaging. It was verified that anthocyanin produced by the expression of R1-nj gene can be detected by fluorescence imaging, and we developed a high-throughput method for discriminating between haploid and diploid seeds. Compared with discriminating with naked eye, this method reduced the time for discriminating haploid and diploid maize by 91.7% and increased selection accuracy by 16.8% for haploid and 2.2% for diploid maize. This method enabled the acquisition of more haploid seeds and improved the efficiency of breeding research by shortening the time involved.

Inhibitory effects of a recombinant viral cystatin protein on cysteine protease catalytic activity and development of Plutella xylostella

Yeongtae Kim,Seonghyun Eom,Yonggyun Kim 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

Cystatins (CSTs) are reversible and competitive inhibitors of C1A cysteine proteases, corresponding to papain-like cathepsins in plants and animals. A viral CST (CpBV-CST1) was identified from a polydnavirus, Cotesia plutellae bracovirus. Our previous study indicated that overexpression of CpBV-CST1 interfered with immune response and development of Plutella xylostella larvae. This study produced a recombinant CpBV-CST1 protein (rCpBV-CST1) using bacterial expression system to analyze its inhibitory activity against cysteine protease and physiological role in the parasitism of an endoparsitoid wasp, Cotesia plutellae. The open reading frame (ORF) of CpBV-CST1 encodes a polypeptide of 138 amino acids (15 kDa). rCpBV-cystatin protein in BL21 STAR (DE3) competent cells containing a recombinant pGEX4T-3:CpBV-CST1 was overexpressed by 0.5 mM IPTG for 4 h. In biological activity assay, partially purified GST-fused rCpBV-CST1 showed inhibitory activity against papain. It also inhibited larval development of P. xylostella in a dose-dependent manner. These results suggest that CpBV-CST1 plays a role in retardation of larval development of P. xylostella during parasitism.

A copy of cystatin from the diamondback moth Plutella xylostella is encoded in the polydnavirus Cotesia plutellae bracovirus

Kim, Yeongtae,Hepat, Rahul,Kim, Yonggyun Korean Society of Applied Entomology 2013 Journal of Asia-Pacific Entomology Vol.20 No.1

Cystatins (CSTs) are reversible and competitive inhibitors of cysteine proteases. Some polydnaviruses encode viral CSTs that have been speculated to play a crucial role in viral pathology. Four CSTs have been reported in the episomal genome of a polydnavirus, Cotesia plutellae (synonymous with C. vestalis) bracovirus (CpBV). These 4 CSTs share high sequence homologies with other bracoviral CSTs. Further sequence analysis showed that 2 of the CpBV-CSTs are identical. The remaining 3 CSTs have been designated CpBV-CST1, CpBV-CST2, and CpBV-CST3. Expression analysis indicated that CpBV-CST2 was not expressed in any stage of Plutella xylostella, either parasitized or non-parasitized by C. plutellae. However, both CpBV-CST1 and CpBV-CST3 were expressed in all stages of P. xylostella. Interestingly, these 2 genes were also expressed in non-parasitized P. xylostella in all developmental stages. A CST sequence from the non-parasitized larva was 100% identical with that of CpBV-CST1 for the entire open reading frame (ORF). To understand the role of CpBV-CST1 in viral pathology, the ORF was cloned into a eukaryotic expression vector and transiently expressed in non-parasitized larvae. The in vivo transient expression lasted for at least 4 days. Under this condition, the treated larvae suffered significant suppression in immune responses and in development. These results suggest that CpBV-CSTs play a crucial role in parasitism, altering host immune and developmental processes by interrupting normal interactions between CSTs and cysteine proteases in P. xylostella.

DEA를 활용한 ERP시스템 도입 중소기업의 운영효율성 측정에 관한 연구

김영태(Yeongtae Kim),유한주(Hanjoo Yoo),송광석(Gwangsuk Song) 한국생산관리학회 2008 韓國生産管理學會誌 Vol.19 No.4

국내 많은 기업들은 기업의 경쟁력을 강화하기 위해 ERP 시스템을 도입하여 왔다. 최근 많은 연구들에 의하면 ERP시스템을 도입한 기업체들은 기업의 자원을 통합하여 관리함으로써 기업체의 생산성을 향상시켜 왔다. 따라서, ERP 시스템을 사용하고 있는 기업체의 운용효율성을 측정하는 것은 정보시스템의 생산성 측면에서 매우 중요하다고 할 수 있다. 본 연구의 목적은 자료포락분석(DEA) 기법을 사용하여 중소기업의 운용 효율성을 분석 한 연구로 DEA 모델 중 CCR/BCC모델, 초효율성모델과 여분기반가중치분석모델(weighted SBM)을 사용하여 9개 자동차부품 업체의 운용효율성을 측정하고 비교하였다. 전통적인 효율성 측정방법인 CCR모델 및 BCC모델의 경우 투입 및 산출물의 여분을 고려하지 않는 문제점이 있지만 본 논문에서는 여분기반가중치분석모델(weighted SBM)을 이용하여 효율 및 비효율의 정확한 양을 측정하여 효율적인 기업들의 순위를 분석하였다. Many companies have introduced information systems and the ERP system in order to strengthen their competitiveness. According to a recent paper, companies that introduced the ERP system have improved productivity by integrating and managing company resources. As a result, it is important to measure the operational efficiency of companies utilizing the ERP system. The purpose of this paper is to analyze the operational efficiency of small and medium companies using Data Envelopment Analysis (DEA). We used CCR, BCC,Super-efficiency and Slack based measure (SBM) model in terms of the DEA model to measure the operational efficiency of nine Automobile parts companies. Since the traditional method based on a measurement model(CCR, BCC) has some problems in not taking account of the existence of slack inputs and outputs in several efficient units, we attempted to measure the exact amount of efficiency and inefficiency of the companies and to do a rank analysis of many efficient units through the slacks-based measure model (SBM).

Development of high-throughput phenotyping system for selecting drought-tolerant lines in gene-edited rice

Min Ji Kim,Nyunhee Kim,Eunsook An,Yeongtae Kim,Dong-Kyung Yoon,Hyeonso Ji,Mi Hyun Cho,Jiseon Song,In Sun Yoon,Sang Ryeol Park,Song Lim Kim 한국육종학회 2023 한국육종학회 공동학술발표집 Vol.2023 No.-

폴리드나바이러스 유래 시스타틴 유전자 발현 형질전환 담배 제작

김영태,김은성,박영진,김용균,Kim, Yeongtae,Kim, Eunsung,Park, Youngjin,Kim, Yonggyun 한국응용곤충학회 2015 한국응용곤충학회지 Vol.54 No.1

폴리드나바이러스의 일종인 CpBV (Cotesia plutellae bracovirus) 바이러스 게놈에 포함된 시스타틴(CpBV-CST1) 유전자의 과발현이 곤충의 면역 및 발육을 교란한다. 이 연구는 바이러스 유래 시스타틴의 생물적 기능의 심화 연구와 해충저항성 작물 개발을 위해 담배형질전환체를 구축하는 데 목적을 두었다. 이를 위해 형질전환체를 대상으로 목표유전자의 발현분석과 곤충에 대한 발육억제에 대한 생물검정을 수행했다. 시스타틴 유전자를 pBI121 운반체에 재조합한 pBI121-CST를 제작하고, 이를 아그로박테리움(Agrobacterium tumefasciens) 세균 매개에 의한 담배 형질전환 및 재분화를 유도하여 약 92%의 높은 신초 재분화율을 나타냈다. 이들 재분화된 개체 가운데 담배 genomic DNA에 시스타틴 유전자가 삽입된 형질전환 추정 개체를 PCR 분석법으로 선발하였다. 다시 quantitative real-time PCR (qRT-PCR) 분석을 통해 이들 목표유전자의 발현을 분석하였다. qRT-PCR 결과는 형질전환 추정 개체가 비형질전환체에 비해 유전자 발현이 약 17 배 높게 나타나 형질전환계통에서 목표유전자가 안정적으로 발현되고 있음을 확인했다. 선발된 형질전환담배를 대상으로 갓 부화한 담배나방(Helicoverpa assulta) 1령 유충에 대한 살충효과를 확인하였다. 살충력에 있어서 형질전환계통간의 차이가 있었다. 특히 T9와 T12계통은 섭식 후 7 일차 조사에서 95% 이상의 살충효과를 보였다. 이상의 결과들은 CpBV-CST1이 해충저항성 작물 개발에 필요한 유용 유전자 자원으로서 활용될 수 있음을 제시하고 있다. CpBV (Cotesia plutellae bracovirus) is a polydnavirus and encodes a cystatin (CpBV-CST1) gene. Its overexpression suppresses insect immunity and alters insect developmental processes. This study aimed to construct a genetically modified (GM) tobacco to further explore the physiological function of the viral cystatin and to apply to control insect pests. To this end, the transgenic tobacco lines were screened in expression of the target gene and assessed in insecticidal activity. A recombinant vector (pBI121-CST) was prepared and used to transform a bacterium, Agrobacterium tumefasciens. The transformed bacteria were used to generate transgenic tobacco lines, which were induced to grow callus and resulted in about 92% of shoot regeneration. The regenerated plants were screened by PCR analysis to confirm the insertion of the target gene in the plant genome. In addition, the expression of the target gene was assessed in the regenerated plants by quantitative real-time PCR (qRT-PCR). The qRT-PCR analysis showed that the transgenic line plant expressed the target gene about 17 times more than the control tobacco, indicating a stable insertion and expression of the target gene in the transgenic tobacco line. The insecticidal activity was then analyzed using the screened transgenic tobacco lines against the teneral 1st instar larvae of the oriental tobacco budworm, Helicoverpa assulta. Though there was a variation in the insecticidal efficacy among transgenic lines, T9 and T12 lines exhibited more than 95% mortality at 7 days after feeding treatment. These results suggest that CpBV-CST1 is a useful genetic resource to be used to generate GM crop against insect pests.

Light-Weight Character Recognition Algorithm Based on Normalized Density for Smart Metering Devices

YeongTae Han,M. Y. Abbass,HyungWon Kim 대한전자공학회 2017 대한전자공학회 학술대회 Vol.2017 No.1

Smart metering is one of fast growing applications for low-power wireless sensor networks. Most of wireless sensor nodes are operated by batteries. Transmitting high rate raw visual data over wireless networks often leads to excessive transmit power consumption. It is considered significantly more power efficient for the metering devices to recognize the character images and transmit only the compact recognized characters. We propose a fast character recognition algorithm, which utilizes the notion of normalized density and image segmentation. It can achieve high recognition rate at significantly low computation complexity, and so is well suited for low power smart metering devices. We implemented the proposed algorithm in a C program for a smart metering device using a low power MCU. We demonstrate that the proposed algorithm produces high recognition rate for various images from real water meters.

박층 아스팔트 포장의 장대교량 적용 사례

최영태(Yeongtae Choi),박봉식(Bongsik Park),김기현(Ki-Hyun Kim) 한국강구조학회 2020 韓國鋼構造學會誌 Vol.32 No.3