Computed Tomography Manifestations of Histologic Subtypes of Retroperitoneal Liposarcoma

Lu, Jing,Qin, Qin,Zhan, Liang-Liang,Yang, Xi,Xu, Qing,Yu, Jing,Dou, Li-Na,Zhang, Hao,Yang, Yan,Chen, Xiao-Chen,Yang, Yue-Hua,Cheng, Hong-Yan,Sun, Xin-Chen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Objective: Liposarcoma (LPS) is the most common soft tissue sarcoma and accounts for approximately 20% of all mesenchymal malignancies, often occurring in deep soft tissue of retroperitoneal space. Accurate preoperative diagnosis is therefore necessary. We explored whether computed tomography (CT) could be used to differentiate between the various types of retroperitoneal liposarcoma (RPLS). Method: Forty-seven cases of RPLS, diagnosed surgically and histologically, were analyzed retrospectively. CT features were correlated with postoperative pathological appearance. Results: The study radiologist identified 29, 11, 2, 2 and 3 RPLS as atypical lipomatous tumor/well-differentiated liposarcoma (ALT/WDL), dedifferentiated liposarcoma (DDL), myxoid/round cell liposarcoma (ML/RCL), pleomorphic liposarcoma (PL) and mixed-type liposarcoma. Analysis of CT scans revealed the following typical findings of the different subtypes of RPLS: ALT/WDL was mainly visible as a well-delineated fatty hypodense tumor with uniform density and integrity margin; DDL was marked by the combination of focal nodular density and hypervascularity. ML/RCL, PL and mixed liposarcoma showed malignant biological behaviour and CT findings need further studies. Conclusions: CT scanning can reveal important details including internal components, margins and surrounding tissues. Based on CT findings, tumor type can be roughly evaluated and biopsy location and therapeutic scheme guided.

Neutrophil Count and the Inflammation-based Glasgow Prognostic Score Predict Survival in Patients with Advanced Gastric Cancer Receiving First-line Chemotherapy

Li, Qing-Qing,Lu, Zhi-Hao,Yang, Li,Lu, Ming,Zhang, Xiao-Tian,Li, Jian,Zhou, Jun,Wang, Xi-Cheng,Gong, Ji-Fang,Gao, Jing,Li, Jie,Li, Yan,Shen, Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Purpose: To explore the value of systemic inflammatory markers as independent prognostic factors and the extent these markers improve prognostic classification for patients with inoperable advanced or metastatic gastric cancer (GC) receiving palliative chemotherapy. Methods: We studied the prognostic value of systemic inflammatory factors such as circulating white blood cell count and its components as well as that combined to form inflammation-based prognostic scores (Glasgow Prognostic Score (GPS), Neutrophil-Lymphocyte Ratio (NLR), Platelet Lymphocyte Ratio (PLR), Prognostic Index (PI) and Prognostic Nutritional Index (PNI)) in 384 patients with inoperable advanced or metastatic gastric cancer (GC) receiving first-line chemotherapy. Univariate and multivariate analyses were performed to examine the impact of inflammatory markers on overall survival (OS). Results: Univariate analysis revealed that an elevated white blood cell, neutrophil and/or platelet count, a decreased lymphocyte count, a low serum albumin concentration, and high CRP concentration, as well as elevated NLR/PLR, GPS, PI, PNI were significant predictors of shorter OS. Multivariate analysis demonstrated that only elevated neutrophil count (HR 3.696, p=0.003) and higher GPS (HR 1.621, p=0.01) were independent predictors of poor OS. Conclusion: This study demonstrated elevated pretreatment neutrophil count and high GPS to be independent predictors of shorter OS in inoperable advanced or metastatic GC patients treated with first-line chemotherapy. Upon validation of these data in independent studies, stratification of patients using these markers in future clinical trials is recommended.

Radiosensitivity Enhancement by Arsenic Trioxide in Conjunction with Hyperthermia in the EC-1 Esophageal Carcinoma Cell Line

Cui, Yan-Hui,Liang, Hai-Jun,Zhang, Qing-Qin,Li, Si-Qing,Li, Xiao-Rui,Huo, Xiao-Qing,Yang, Qing-Hui,Li, Wei-Wei,Gu, Jian-Fa,Hua, Qin-Liang,Lu, Ping,Miao, Zhan-Hui Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: To explore the effect on radiosensitivity of arsenic trioxide ($As_20_3$) in conjunction with hyperthermia on the esophageal carcinoma EC-1 cell line. Method: Inhibition of EC-1 cell proliferation at different concentrations of $As_20_3$ was assessed using the methyl thiazolyl blue colorimetric method (MTT method), with calculation of $IC_{50}$ value and choice of 20% of the $IC_{50}$ as the experimental drug concentration. Blank control, $As_20_3$, hyperthermia, radiotherapy group, $As_20_3$ + hyperthermia, $As_20_3$ + radiotherapy, hyperthermia + radiotherapy and $As_20_3$ + hyperthermia + radiotherapy groups were established, and the cell survival fraction (SF) was calculated from flat panel colony forming analysis, and fitted by the 'multitarget click mathematical model'. Flow cytometry (FCM) was used to detect changes in cell apoptosis and the cell cycle. Results: $As_20_3$ exerted inhibitory effects on proliferation of esophageal carcinoma EC-1 cells, with an $IC_{50}$ of 18.7 ${\mu}mol/L$. After joint therapy of $As_20_3$ + hyperthermia + radiotherapy, the results of FCM showed that cells could be arrested in the $G_2$/M phase, and as the ratio of cells in $G_0/G_1$ and S phases decreased, cell death became more pronounced. Conclusion: $As_20_3$ and hyperthermia exert radiosensitivity effects on esophageal carcinoma EC-1 cells, with synergy in combination. Mechanistically, $As_20_3$ and hyperthermia mainly influence the cell cycle distribution of EC-1 esophageal carcinoma cells, decreasing the repair of sublethal damage and inducing apoptosis, thereby enhancing the killing effects of radioactive rays.

Effects of different culture systems on the culture of buffalo (Bubalus bubalis) spermatogonia stem cell-like cells in vitro

Ting-Ting Li,Shuang-Shuang Geng,Hui-Yan Xu,Ao-Lin Luo,Peng-Wei Zhao,Huan Yang,Xing-Wei Liang,Yang-Qing Lu,Xiao-Gan Yang,Ke-Huan Lu 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.1

Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) in vitro are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (KnockOut Serum Replacement) materials on the in vitro culture of buffalo SSC-like cells. Significantly more DDX4- and UCHL1-positive cells (cultured for 2 days at passage 2) were observed in the defined materials culture system than in the undefined materials system (p < 0.01), and these cells were maintained for a longer period than those in the culture system with undefined materials (10 days vs. 6 days). Furthermore, NANOS2 (p < 0.05), DDX4 (p < 0.01) and UCHL1 (p < 0.05) were expressed at significantly higher levels in the culture system with defined materials than in that with undefined materials. Induction with retinoic acid was used to verify that the cultured cells maintained SSC characteristics, revealing an SCP3+ subset in the cells cultured in the defined materials system. The expression levels of Stra8 (p < 0.05) and Rec8 (p < 0.01) were significantly increased, and the expression levels of ZBTB16 (p < 0.01) and DDX4 (p < 0.05) were significantly decreased. These findings provided a clearer research platform for exploring the mechanism of buffalo SSCs in vitro.

Polymorphisms in the Perilipin Gene May Affect Carcass Traits of Chinese Meat-type Chickens

Lu Zhang,Qing Zhu,Yiping Liu,Elizabeth R. Gilbert,Diyan Li,Huadong Yin,Yan Wang,Zhiqin Yang,Zhen Wang,Yuncong Yuan,Xiao-Ling Zhao 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.6

Improved meat quality and greater muscle yield are highly sought after in high-quality chicken breeding programs. Past studies indicated that polymorphisms of the Perilipin gene (PLIN1) are highly associated with adiposity in mammals and are potential molecular markers for improving meat quality and carcass traits in chickens. In the present study, we screened single nucleotide polymorphisms (SNPs) in all exons of the PLIN1 gene with a direct sequencing method in six populations with different genetic backgrounds (total 240 individuals). We evaluated the association between the polymorphisms and carcass and meat quality traits. We identified three SNPs, located on the 5′ flanking region and exon 1 of PLIN1 on chromosome 10 (rs315831750, rs313726543, and rs80724063, respectively). Eight main haplotypes were constructed based on these SNPs. We calculated the allelic and genotypic frequencies, and genetic diversity parameters of the three SNPs. The polymorphism information content (PIC) ranged from 0.2768 to 0.3750, which reflected an intermediate genetic diversity for all chickens. The CC, CT, and TT genotypes influenced the percentage of breast muscle (PBM), percentage of leg muscle (PLM) and percentage of abdominal fat at rs315831750 (p<0.05). Diplotypes (haplotype pairs) affected the percentage of eviscerated weight (PEW) and PBM (p<0.05). Compared with chickens carrying other diplotypes, H3H7 had the greatest PEW and H2H2 had the greatest PBM, and those with diplotype H7H7 had the smallest PEW and PBM. We conclude that PLIN1 gene polymorphisms may affect broiler carcass and breast muscle yields, and diplotypes H3H7 and H2H2 could be positive molecular markers to enhance PEW and PBM in chickens.

Inhibitory effect of StCYP707A1 gene on tuberization in transgenic potato

Lu Liu,Rui-Jie Zhang,Wen-jiao Zhu,Xin-Ru Liu,Ke Shi,Min Chen,Qing Yang 한국식물생명공학회 2017 Plant biotechnology reports Vol.11 No.4

Tuber formation of potato is a complex biological process and is regulated by many factors including phytochrome, hormones, transcription factors, RNAs, microRNAs, etc. In our previous study, CYP707A1, an ABA 80-hydroxylase gene, was down-regulated in the StCOL antisense transgenic potato stolons, but its relation with potato tuberization has not been studied yet. In this study, to investigate the role of this gene in potato tuberization, we cloned a CYP707A1 gene from potato cultivar De ´sire ´e and constructed the StCYP707A1 over-expression and anti-sense potato lines by Agrobacterium-mediated transformation. In total, four over-expression transgenic lines and nine anti-sense transgenic lines were confirmed by PCR analysis. Semi-quantitative RT-PCR and qRTPCR analysis showed that the level of StCYP707A1 transcripts was significantly higher in the over-expression lines (A7 and A9) and lower in the anti-sense lines (F2 and F8), compared to the wild-type control. We further analyzed tuber formation in the transgenic lines and the wild-type control. The result showed that tuber yield per plant and average tuber weight were decreased in A7 and A9 and increased in F2. We also measured the content of ABA and GA3 in transgenic lines. ABA level was reduced in A7 and A9 and increased in F2. Contrariwise, the concentration of GA3 was higher in A7 and A9, and lower in F2 than in wild-type control. These results indicate that StCYP707A1 negatively affects potato tuberization through ABA regulation on gibberellic acid concentration.

The In Situ and In Vivo Study on Enhancing Effect of Borneol in Nasal Absorption of Geniposide in Rats

Yang Lu,Xiaolan Chen,Shouying Du,Qing Wu,Zongling Yao,Yongsong Zhai 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.5

The objective of this research was to study the in situ and in vivo nasal absorption of Geniposide (Ge) co-administered with borneol. A rat in situ nasal perfusion technique with a novel volumeadjusted calculation was used to examine the absorption rate and extent of Ge. The influence of different experimental conditions such as purity of extract, drug concentration, co-administration with synthetic borneol or natural borneol were also investigated. Results indicated nasal absorption of Ge was primarily by passive diffusion that resembled first order kinetics. Following co-administration with borenol, the drug absorption was increased by 1.4 and 1.7 folds for natural borneol and synthetic borneol , respectively. However, the effect of other factors on drug absorption was not significant. In addition, it was also observed that there is a positive correlation between the absorption of water and Ge by the nasal route. In vivo studies carried out in rats where Ge was co-administered with NB and the pharmacokinetic profile obtained following intranasal administration were compared with those after intravenous administration. The bioavailability of Ge by intranasal was 101.5% and Tmax was 2.04 ± 0.64 min. MRT was 218.7 ± 74.1 min and 44.4 ± 8.9 min for intranasal and intravenous, respectively. Combined with the borneol,Ge can be promptly and thoroughly absorbed intranasally in rats.

Is Hepatectomy for Huge Hepatocellular Carcinoma (≥10cm in Diameter) Safe and Effective? A Single-center Experience

Yang, Jian,Li, Chuan,Wen, Tian-Fu,Yan, Lu-Nan,Li, Bo,Wang, Wen-Tao,Yang, Jia-Yin,Xu, Ming-Qing Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Background: This retrospective study aimed to validate the safety and effectiveness of hepatectomy for huge hepatocellular carcinoma (HCC). Materials and Methods: Data of patients who underwent hepatectomy for HCC between January 2006 and December 2012 were reviewed. The patients were divided into three groups: huge HCC(${\geq}10cm$ in diameter), large HCC(${\geq}5$ but<10 cm in diameter) and small HCC(<5cm in diameter). Results: Characteristics of pre-operative patients in all three groups were homogeneously distributed except for alpha fetal protein (AFP)(p<0.001).The 30, 60, 90-day post-operative mortality rates were not different among the three groups (p=0.785, p=0.560, and p=0.549). Laboratory data at 1, 3, and 7 days after surgery also did not vary. The 5-year overall survival (OS) and 5-year disease-free survival (DFS) rates in the huge and large HCC groups were lower than that of the small HCC group (OS: 32.5% vs 36.3% vs 71.2%, p=0.000; DFS: 20.0% vs 24.8% vs 40.7%, p=0.039), but there was no difference between the huge and large HCC groups (OS: 32.5% vs 36.3%, p=0.667; DFS: 20.0% vs 24.8%, p=0.540). In multivariate analysis, five independent poor prognostic factors that affected OS were significantly associated with worse survival (p<0.05), namely, AFP level, macrovascular invasion, Edmondsone Steiner grade, surgical margin and Ishak score. AFP level, macrovascular invasion, microvascular invasion, and surgical margin influenced disease-free survival independently (p<0.05). Conclusions: The safety of hepatectomy for huge HCC is similar to that for large and small HCC; and this approach for huge HCC may achieve similar long-term survival and disease-free survival as for large HCC.

Analysis on Double Rain Belts of a Rainstorm in Liaoning

YANG Qing,YAN Qi,CHEN Li-qiang,GAO Song-ying,LI Shuang,CUI Sheng-quan,LU Bing-hong 한국기상학회 2015 한국기상학회 학술대회 논문집 Vol.2015 No.10

Exogenous Spermidine Promoted Ca2+ Absorption in Lettuce Roots and Reduced the Incidence of Tipburn

Qiao Yang,Na Lu,Lei Wang,Xiao-qing Huang,Da-quan Yang,Jin Sun 한국원예학회 2018 원예과학기술지 Vol.36 No.5

A physiological disorder, called tipburn, commonly occurs in lettuce (Lactuca sativa L.), particularly in greenhouse cultivation and in hot seasons, and reduces its marketability. Calcium (Ca) deficiency is the main cause of tipburn injury. In the present study, we investigated the effect of exogenous spermidine (Spd) on the occurrence of tipburn in hydroponically cultivated head lettuce (var. capitata L.) and elucidated the role of Ca ions (Ca2+) in its prevention with respect to absorption and transport. Ca deficiency in the nutrient solution resulted in reduced uptake of Ca2+ in lettuce roots, thereby resulting in Ca deficiency in leaves, leading to leaf senescence and tipburn. An application of exogenous Spd promoted Ca2+ uptake in lettuce roots under Ca deficiency and promoted Ca2+ transportation to leaves, especially to inner leaves, thus delaying leaf senescence and reducing the incidence of tipburn. The results indicated that application of exogenous Spd is an effective method to decrease tipburn in lettuce caused by Ca deficiency.