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뇌동맥류 파열 환자에서 조기 수술 대상 선택 : 10년간 1026 치험예를 바탕으로한 분석 Based on 10-year Experience with 1026 Patients
양국희,박현선,신용삼,주진양,허승곤,이규창 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.11
The purpose of this study was to set the guidelines for selection of patients to do early surgery in ruptured intracranial aneurysm. We assessed 706 patients with single rupture and without large hematoma, who underwent aneurysm surgery from 1985 to 1995. The male and female ratio was 1 : 1.5 Among the 706 patients, early surgery was performed in 214 cases. The results of early surgery were good in 193 cases(90 2%). fair in 13 cases(6.0%), poor in 1 case(0.5%) and dead in 7 cases(3.3%). The rate of dead outcome in the early surgery group was higher compared to other timing groups. The Fisher group 1, 2 and 3 revealed good outcome in early surgery group ; 92.6%, 96.3%, 88.8% respectively. The incidence of delayed ischemic deficits(DID) of early surgery group was same as other groups. However, in Fisher group 3, the incidence of DID was significantly low. 32. 5%, in early surgery group. It is suggested that the criteria of selection of early surgery in patients with ruptured intracranial aneurysm would include as follows : 1) patients with good clinical grade. 2) poor grade patients with marked irritability. acute hydrocephalus, and poorly controlled hypertension, 3) none-complex aneurysm requiring less brain retraction, dissection and brief temporary clipping. 4) age under 60 or over 60 with good physical status, and 5) Fisher group 3 requiring cisternal larvage and anticipated triple-H therapy.
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Biochemical Characterization of Homoserine Dehydrogenase from Pseudomonas aeruginosa
Do Hyeon Kim,Quyet Thang Nguyen,Jin Kuk Yang 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.2
Homoserine dehydrogenase (HSD) catalyzes the reduction of l-aspatate-4-semaldehyde (l-ASA) to l-homoserine (l-HSE) or oxidation reversely, which is a part of the aspartate pathway synthesizing threonine, isoleucine, and methionine in vivo. HSD has gained much interest in medical application since HSD is a well-established target for pesticides and antibiotics. In addition, HSD is also valuable in industrial application for l-lysine production. In this study, HSD from Pseudomonas aeruginosa (PaHSD) was overexpressed in Escherichia coli, and purified to homogeneity for molecular and biochemical characterization. PaHSD exhibits the comparable activity of l-HSE oxidation for both types of cofactors, NAD+ and NADP+, but at the same time shows interesting differences. The kcat/Km value of NADP+ was ~1.8 times larger than that of NAD+, while the kcat/Km value of l-HSE was ~1.4 times larger with NAD+ than with NADP+. Notably, the Km value is about three times smaller for NADP+, while Vmax is about 1.7 times larger for NAD+, which implies that while NADP+ binds more strongly to the active site, NAD+ has a faster turnover. Size-exclusion chromatography analysis showed PaHSD forms tetramers, as opposed to HSDs from other species forming dimers. In the circular dichroism analysis, PaHSD showed a typical spectrum of α/? structure, and its melting temperature was determined to be 50.5 °C from the thermal denaturation test at 222?nm. The molecular and biochemical features of PaHSD revealed in this study provide the basis for the future study of amino acid metabolism, and its application to industrial and medical purposes.
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김현영 ( Hyeon Yeong Kim ),황양인 ( Yang In Hwang ),국원근 ( Won Kwen Kuk ) 한국산업위생학회 2012 한국산업보건학회지 Vol.22 No.2
Objectives: Epichlorohydrin is a material that has been predicted to have high volatility and strong toxicity and is used normally in working area. Therefore, the hazardous and dangerous level and the foreign management system about epichlorohydrin should be invested at home and abroad and through hazard assessment for occupational environment measurement and exposure status of industrial workers in domestic working area. Methods: To assess risk and to suggest Development and Adoption to prevent health damage of workers owing to the epichlorohydrin exposure, the hazardousness and dangerousness of epichlorohydrin and its practical examples and regulation level for domestic and abroad health impairment are researched on the base of various references. Results: The epichlorohydrin caused skin and mucus membrane irritation, respiratory paralysis, kidney and live damage under the influence of acute toxicity and in animal study, it was confirmed as a doubtful carcinogenic substance to trigger reducement of sperm number and reproduction ability, abnormal spermatogenesis, mutagen, increase of forestomach epithelium and occurrence of papilloma and so on, as well as it induced stimulus asthma and allergic contact dermatitis for exposure workers. Conclusions: Epichlorohydrin was found to occur allergic contact dermatitis, carcinogenesis doubt and reproduction toxicity and was verified as a material which would be established reinforcement of management level to care health of handlers, such as denotement dangerousness of skin absorption.
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Molecular and Enzymatic Features of Homoserine Dehydrogenase from Bacillus subtilis
( Do Hyeon Kim ),( Quyet Thang Nguyen ),( Gyeong Soo Ko ),( Jin Kuk Yang ) 한국미생물생명공학회(구 한국산업미생물학회) 2020 Journal of microbiology and biotechnology Vol.30 No.12
Homoserine dehydrogenase (HSD) catalyzes the reversible conversion of L-aspartate-4- semialdehyde to L-homoserine in the aspartate pathway for the biosynthesis of lysine, methionine, threonine, and isoleucine. HSD has attracted great attention for medical and industrial purposes due to its recognized application in the development of pesticides and is being utilized in the large scale production of L-lysine. In this study, HSD from Bacillus subtilis (BsHSD) was overexpressed in Escherichia coli and purified to homogeneity for biochemical characterization. We examined the enzymatic activity of BsHSD for L-homoserine oxidation and found that BsHSD exclusively prefers NADP<sup>+</sup> to NAD<sup>+</sup> and that its activity was maximal at pH 9.0 and in the presence of 0.4 M NaCl. By kinetic analysis, K<sub>m</sub> values for L-homoserine and NADP<sup>+</sup> were found to be 35.08 ± 2.91 mM and 0.39 ± 0.05 mM, respectively, and the Vmax values were 2.72 ± 0.06 μmol/min<sup>-1</sup> mg<sup>-1</sup> and 2.79 ± 0.11 μmol/ min<sup>-1</sup> mg<sup>-1</sup>, respectively. The apparent molecular mass determined with size-exclusion chromatography indicated that BsHSD forms a tetramer, in contrast to the previously reported dimeric HSDs from other organisms. This novel oligomeric assembly can be attributed to the additional C-terminal ACT domain of BsHSD. Thermal denaturation monitoring by circular dichroism spectroscopy was used to determine its melting temperature, which was 54.8°C. The molecular and biochemical features of BsHSD revealed in this study may lay the foundation for future studies on amino acid metabolism and its application for industrial and medical purposes.
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Ammonium nitrate의 유해성과 작업환경 관리
김현영 ( Hyeon Yeong Kim ),황양인 ( Yang In Hwang ),국원근 ( Won Kwen Kuk ) 한국산업위생학회 2012 한국산업보건학회지 Vol.22 No.3
Objectives: The purpose of this study is the work environment management method through risk assessment and investigation of the work place that deals with Ammonium nitrate, based on information in and outside the country. Methods: This study suggests method of work environment management through risk assessment and investigation of the work place that deals with Ammonium nitrate, and finds out cases of Ammonium nitrate causing hazard, danger and health risk, based on literature investigation. Results: Rats exposed repeatedly to LD50 2,217 mg/kg(rat), LC50 88.8 mg/L(rat, skin) which cause high level of skin irritation, reported 1 mg/m3 of NOAEL, while LOAEL was less than 100 mg/kg for the rats orally administered with the LD50 2,217 mg/kg(rat), LC50 88.8 mg/L(rat, skin), for 13 weeks, Domestically 31,640 ton/y of ammonium nitrate has been used in 22 workplace and the result of workplace assessment was 0.0171-0.9983 mg/m3, ADD was 8.77-59.63ug/kg-day according to the exposure scenario. In other words the result of the risk assessment goes beyond the ``standard 1``. Conclusions: Ammonium nitrate creates a high level of irritation and toxicity when coming in breathe it or contact with skin, and is classified as category3 of GHS and specific target organ toxicant (irritating respiratory system), Exposure level at work places needs to be maintained under 1 mg/m3, to prevent workers from being damaged.
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임성국 ( Sung Kuk Im ),김문현 ( Moon Hyeon Kim ),양원호 ( Won Ho Yang ) 대구가톨릭대학교 자연과학연구소 2007 자연과학연구논문집 Vol.5 No.1
Human exposure is defined as the contact at one or more boundaries such as mouth and skin between a human and a pollutant at a specific concentration for a period of time. Instead of focusing purely on the sources of pollution or their transport and movement through the environment, this study focuses on humans as the receptors of pollutants considering daily activities. This study presents personal exposure concentration distributions of nitrogen dioxide(NO2) in the iron mill 30 workers during working 5 days. In addition this paper presents corresponding concentration distributions measured in residential indoor, residential outdoor, and workplace microenvironments and their contribution to personal exposure concentrations. Personal exposure to NO2 was significantly correlated with workplace for all pollutants. This result indicates that participants in this study were mainly exposed in workplace in spite of working hours.
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임성국(Sung-Kuk Yim),김문현(Moon-Hyeon Kim),손부순(Bu-Soon Son),양원호(Won-Ho Yang) 한국실내환경학회 2007 한국실내환경학회지 Vol.4 No.1
Burning mosquito coils in indoor environments maygenerate smoke that can control mosquito effectively. This practice has been used in numerous households in Korea. However the smoke may contain air pollutants of health concern. We conducted the present study to characterize the emission from two common brands of mosquito coils from Vietnam and Malaysia, respectively. We measured mass emission of air pollutants of nitrogen oxides (NOx), fine particulate(PM₂.?), formaldehyde (HCHO), total volatile organic compounds (TVOCs), carbon monoxide (CO) and carbon dioxide (CO₂) in completely closed chamber. Air pollutants concentrations resulting from burning mosquito coils could substantially exceed health-based air quality standards orguidelines. Under the same condition, air pollutants were measured by cigarette smoking to compare mosquito coil. Burning one mosquito coil would release the same amount of PM₂.? mass as burning 20~58 cigarettes. The emission of HCHO from burning one coil can be as high as that released from burning 27 cigarettes.
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