Estrogen weakens muscle endurance via estrogen receptor-p38 MAPK-mediated orosomucoid (ORM) suppression

Yang Sun,Zhen Qin,Jing-jing Wan,Peng-yuan Wang,Yi-Li Yang,Jian-Guang Yu,Bo-Han Hu,Ding-Feng Su,Zhu-Min Luo,Xia Liu 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Gender differences in fatigue manifest as females being more prone to feel exhaustion and having lower muscle endurance. However, the mechanisms of these effects remain unclear. We investigated whether orosomucoid, an endogenous anti-fatigue protein that enhances muscle endurance, is involved in this regulation. Female rats exhibited lower muscle endurance, and this gender difference disappeared in orosomucoid-1-deficient mice. Female rats also exhibited weaker orosomucoid induction in serum, liver and muscle in response to fatigue compared with male rats. Ovariectomy elevated orosomucoid levels and increased swimming time, and estrogen replenishment reversed these effects. Exogenous estrogen treatment in male and female mice produced opposite effects. Estrogen decreased orosomucoid expression and its promoter activity in C2C12 muscle and Chang liver cells in vitro, and estrogen receptor or p38 mitogen-activated protein kinase blockade abolished this effect. Therefore, estrogen negatively regulates orosomucoid expression that is responsible for the weaker muscle endurance in females.

Vitexicarpin Induces Apoptosis in Human Prostate Carcinoma PC-3 Cells through G2/M Phase Arrest

Meng, Fan-Min,Yang, Jing-Bo,Yang, Chun-Hui,Jiang, Yu,Zhou, Yong-Feng,Yu, Bo,Yang, Hong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Vitexicarpin (3', 5-dihydroxy-3, 4', 6, 7-tetramethoxyflavone), a polymethoxyflavone isolated from Viticis Fructus (Vitex rotundifolia Linne fil.), has long been used as an anti-inflammatory herb in traditional Chinese medicine. It has also been reported that vitexicarpin can inhibit the growth of various cancer cells. However, there is no report elucidating its effect on human prostate carcinoma cells. The aim of the present study was to examine the apoptotic induction activity of vitexicarpin on PC-3 cells and molecular mechanisms involved. MTT studies showed that vitexicarpin dose-dependently inhibited growth of PC-3 cells with an $IC_{50}{\sim}28.8{\mu}M$. Hoechst 33258 staining further revealed that vitexicarpin induced apoptotic cell death. The effect of vitexicarpin on PC-3 cells apoptosis was tested using prodium iodide (PI)/Annexin V-FITC double staining and flow cytometry. The results indicated that vitexicarpin induction of apoptotic cell death in PC-3 cells was accompanied by cell cycle arrest in the G2/M phase. Furthermore, our study demonstrated that vitexicarpin induction of PC-3 cell apoptosis was associated with upregulation of the proapoptotic protein Bax, and downregulation of antiapoptotic protein Bcl-2, release of Cytochrome c from mitochondria and decrease in mitochondrial membrane potential. Our findings suggested that vitexicarpin may become a potential leading drug in the therapy of prostate carcinoma.

A Novel Endo-Polygalacturonase from Penicillium oxalicum: Gene Cloning, Heterologous Expression and Its Use in Acidic Fruit Juice Extraction

( Bo Lu ),( Liang Xian ),( Jing Zhu ),( Yunyi Wei ),( Chengwei Yang ),( Zhong Cheng ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.4

An endo-polygalacturonase (endo-PGase) exhibiting excellent performance during acidic fruit juice production would be highly attractive to the fruit juice industry. However, candidate endo-PGases for this purpose have rarely been reported. In this study, we expressed a gene from Penicillium oxalicum in Pichia pastoris. The recombinant enzyme PoxaEnPG28C had an optimal enzyme activity at pH 4.5 and 45℃ and was stable at pH 3.0-6.5 and < 45℃. The enzyme had a specific activity of 4,377.65 ± 55.37 U/mg towards polygalacturonic acid, and the K_m and V_max values of PoxaEnPG28C were calculated as 1.64 g/l and 6127.45 U/mg, respectively. PoxaEnPG28C increased the light transmittance of orange, lemon, strawberry and hawthorn juice by 13.9 ± 0.3%, 29.4 ± 3.8%, 95.7 ± 10.2% and 79.8 ± 1.7%, respectively; it reduced the viscosity of the same juices by 25.7 ± 1.6%, 52.0 ± 4.5%, 48.2 ± 0.7% and 80.5 ± 2.3%, respectively, and it increased the yield of the juices by 24.5 ± 0.7%, 12.7 ± 2.2%, 48.5 ± 4.2% and 104.5 ± 6.4%, respectively. Thus, PoxaEnPG28C could be considered an excellent candidate enzyme for acidic fruit juice production. Remarkably, fruit juice production using hawthorn as an material was reported for the first time.

Callus induction and high-efficiency plant regeneration via somatic embryogenesis in Papaver nudicaule L., an ornamental medicinal plant

Yang, Jing Li,Zhao, Bo,Seong, Eun-Soo,Kim, Myong-Jo,Kang, Won-Hee,Kim, Na-Young,Yu, Chang-Yeon,Li, Cheng Hao The Korean Society of Plant Biotechnology 2010 Plant biotechnology reports Vol.4 No.4

We describe culture conditions for a high-efficiency in vitro regeneration system of Papaver nudicaule through somatic embryogenesis and secondary somatic embryogenesis. The embryogenic callus induction rate was highest when petiole explants were cultured on Murashige and Skoog (MS) medium containing 1.0 mg $1^{-1}$ ${\alpha}$-naphthaleneacetic acid (NAA) and 0.1 mg $1^{-1}$ 6-benzyladenine (BA) (36.7%). When transferred to plant growth regulator (PGR)-free medium, 430 somatic embryos formed asynchronously from 90 mg of embryogenic callus in each 100-ml flask. Early-stage somatic embryos were transferred to MS medium containing 1.0 mg $1^{-1}$ BA and 1.0 mg $1^{-1}$ NAA to germinate at high frequency (97.6%). One-third-strength MS medium with 1.0% sucrose and 1.0 mg $1^{-1}$ $GA_3$ had the highest frequency of plantlet conversion from somatic embryos (91.2%). Over 90% of regenerated plantlets were successfully acclimated in the greenhouse. Secondary somatic embryos were frequently induced directly when the excised hypocotyls of the primary somatic embryos were cultured on MS medium without PGRs. Sucrose concentration significantly affected the induction of secondary embryos. The highest induction rate (89.5) and number of secondary somatic embryos per explant (9.3) were obtained by 1% sucrose. Most secondary embryos (87.2-94.3%) developed into the cotyledonary stage on induction medium. All cotyledonary secondary embryos were converted into plantlets both in liquid and on semisolid 1/3-strength MS medium with 1.0% sucrose.

Effects of transcription factor SOX11 on the biological behavior of neuroblastoma cell and potential regulatory mechanism

Jing-Ru Huang,Yong Li,Peng Chen,Ji-Xiu Wei,Xia Yang,Qiong-Qian Xu,Jia-Bo Chen 대한외과학회 2024 Annals of Surgical Treatment and Research(ASRT) Vol.106 No.5

Purpose: This study aimed to analyze the expression and prognosis of SRY-box transcription factor 11 (SOX11) in neuroblastoma (NB), as well as the biological function and potential regulatory mechanism of SOX11 in NB. Methods: Public RNA sequencing was used to detect the expression level of SOX11. The Kaplan-Meier curve and hazard ratios (HR) were used to determine the prognostic value of SOX11 in NB. Functional analyses were performed using CCK8, wound healing assay, and transwell invasion assay. Finally, the potential target genes of SOX11 were predicted by Harmonizonme (Maayan Laboratory) and Cistrome Data Browser (Cistrome Project) database to explore the potential molecular mechanism of SOX11 in NB. Results: Compared with normal adrenal tissue, the expression of SOX11 in NB tissue was significantly upregulated. The Kaplan-Meier curve showed that high expression of SOX11 was associated with poor prognosis in children with NB (HR, 1.719; P = 0.049). SOX11 knockdown suppressed the migration capacity of SK-N-SH cells but did not affect proliferation and invasion capacity. Enhancer of zeste homolog 2 (EZH2) may be a potential downstream target gene for the transcription factor SOX11 to play a role in NB. Conclusion: The transcription factor SOX11 was significantly upregulated in NB. SOX11 knockdown suppressed the migration capacity of NB cell SK-N-SH. SOX11 may promote the progression of NB by targeting EZH2.

Vibration diagnostic system of rotating machinery using self-organizing feature map and wavelet transform

Yang, Bo-suk,Lim, Dong-soo,An, Jing-long,Kim, Dong-jo 부경대학교 음향진동공학연구소 2000 音響振動工學硏究所 硏究報告 Vol.5 No.-

Diversity Distributions and the Anthocyanin Associations of Fungal Endophytes in Diferent Colored Grapevine Leaves

Jing‑Chao Chen,Man Yu,Fang Liu,Jin‑Zhuo Qu,Xiao‑Xia Pan,Han‑Bo Zhang,Ming‑Zhi Yang 한국식물학회 2020 Journal of Plant Biology Vol.63 No.2

Anthocyanins composed the major pigments which conferred sensory and chemical values to red grapes and wines. Multiple factors involved in grape anthocyanin biosynthesis have been greatly covered to date. However, the endophytes which closely associate with plants, their possible roles in plant coloration are still under clear. Our present research frstly investigated the diversity distributions of culturable fungal endophytes (CFE) in diferent colored grapevine leaves which harvested from same vineyard and cultivar, and then proposed method to analyze the possible efects of these fungal endophytes on anthocyanin concentrations of grape cells. In totally 532 endophytic fungal isolates, 19 OTUs belong to 13 genera were isolated from fve color diferent grapevine leaves. Obvious leaf color specifcity of CFE communities were observed distribution in diferent colored grapevine leaves in this experiment. Assessing the infuences of those isolated CFEs on anthocyanin concentrations of a teinturier grape pulp cells via dual culture system revealed that anthocyanin promotion fungal strains were more possibly isolated from red and purple colored grape leaves. And elite CFE strains such as DQ53 and DQ55, both belong to the fungal genus Epicoccum, which conferred signifcant promotion efects on grape cellular anthocyanin contents were selected out. And the transcriptions of anthocyanin biosynthesis association genes MYBA1, UFGT and F3′5′H in grape cells, were signifcantly infuenced by these endophytic fungi, furtherly implicated the involvements of these genes in fungal endophytes-mediated grape cell anthocyanin promotion process. Additionally, endophytic fungi triggered anthocyanin promotion in grape cells seem independently to that of the light mediated anthocyanin synthesis pathway. This work demonstrated that beside other environmental factors, endophytes may also involve in grape anthocyanin metabolisms. And the study provided methods and clues to explore fungal endophytes in colored grape leaves which possibly applied in grape pigmentation processing.

Effect of L-carnitine on sperm quality during liquid storage of boar semen

Yang Kang,Wang Na,Guo Hai-Tao,Wang Jing-Ran,Sun Huan-Huan,Sun Liang-Zhen,Yue Shun-Li,Zhou Jia-Bo 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.11

Objective: This study was conducted to investigate the effect of L-carnitine on the pig semen characteristics during storage. Methods: Spermatozoa samples were examined for spermatozoa quality and then randomly divided into 5 groups: 0 (control), 12.5, 25, 50, and 100 mM L-carnitine. Sperm motility, plasma membrane integrity and antioxidant parameters (total reactive oxygen species, total antioxidant capacity, and malondialdehyde) were evaluated after 0, 3, 5, and 10 day cooled-storage at 17°C. Moreover, ATP content, mitochondria activity as well as sperm-binding and in vitro fertilizing ability of preserved boar sperm were also investigated. Results: Supplementation with 50 mM L-carnitine could effectively maintain boar sperm quality parameters such as sperm motility and membrane integrity. Besides, we found that L-carnitine had positive effects on boar sperm quality mainly through improving antioxidant capacities and enhancing ATP content and mitochondria activity. Interestingly, by assessing the effect of L-carnitine on sperm fertility and developmental potential, we discovered that the extender containing L-carnitine could improve sperm quality and increase the number of sperms bounding to zona pellucida, without improving in vitro fertility and development potential. Conclusion: These findings suggested that the proper addition of L-carnitine to the semen extender improved boar sperm quality during liquid storage at 17°C.

Long noncoding RNA UCA1 inhibits ischaemia/reperfusion injury induced cardiomyocytes apoptosis via suppression of endoplasmic reticulum stress

Jing Chen,Qi Hu,Bo‑fang Zhang,Xiao‑pei Liu,Shuo Yang,Hong Jiang 한국유전학회 2019 Genes & Genomics Vol.41 No.7

Background Ischemia heart disease is one of the major causes of death worldwide which often associated with tissue infarction and limit the recovery of function. Multiple factors involved in the I/R-induced cardiomyocyte dysfunction which were consistent with a role of oxidative stress and altered endothelium-dependent responses. However, the pathogenic mechanisms in I/R injury remain unclear. Materials and methods The H9C2 cells were in the ischaemia/reperfusion (I/R) condition. After I/R, the cells were transfected with or without adenovirus-urothelial carcinoma associated 1(Ad-UCA1). Then qRT-PCR analysis was performed to quantify mRNA expression of different treatment groups. Cell apoptosis rate was assessed using flow cytometry and ER stress biomarker expression were measured by immunoblotting. Intracellular and mitochondrial ROS generation were assayed by fluorescence microscope after staining with the DCFDA or MitoSOX. Results I/R conditions trigger lncRNAs UCA1 expression, cellular and mitochondria ROS production, resulting in cell apoptosis through the induction of oxidative and ER stress. Overexpression of UCA1 protects H9C2 cells from I/R-induced ER stress and cell apoptosis. Moreover, UCA1 might be a potential regulator in the protective effect of I/R‑induced oxidative stress and mitochondria dysfunction. Subsequently, ER stress inhibitor attenuated the effect of siUCA1 induced injury in H9C2 cells. Conclusion The expression of UCA1 against I/R induced oxidative stress and mitochondria dysfunction via suppression of endoplasmic reticulum stress. UCA1 might be a biomarker to improved diagnosis of I/R injury.

Experimental Study on Shear Behavior of Bolted Cement Mortar Blocks under Constant Normal Stiffness

Bo Meng,Hongwen Jing,Shengqi Yang,Tao Cui,Biao Li 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.8

In this paper, the influence of bolting on shear mechanical behavior of cement mortar specimens were studied. First, a shear test apparatus which could provide CNS (constant normal stiffness) condition was designed. Then, more than 140 bolted and non-bolted cement mortar blocks (100 × 100 × 100 mm) were prepared. The strengthening effect of bolts with diameters of 0 − 10 mm on cement mortar specimens was studied under different initial normal loads ranging from 20 kN to 60 kN and constant normal stiffness of 420 kN/mm. Two simplified models of shear behavior of reinforced cement mortar blocks were proposed. The most important influence of bolt on the cement mortar blocks was to strengthen the post-peak shear strength rather than the peak shear strength, and the strengthening effect was reduced under high initial normal force. The cohesion of cement mortar blocks was reduced while internal friction angle was increased in residual sliding stage by bolts. The dilation of reinforced cement mortar specimens in postpeak shear stage was significantly reduced due to bolting, and the larger the bolt diameter, the greater the reduction in the block dilation.