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김형신,정민민,박흠기,윤양호,이화자 濟州大學校 基礎科學硏究所 2002 基礎科學硏究 Vol.15 No.2
우리 나라 동해안에 위치한 화진포에서 채집된 요각류, Paracyclopina nana의 생물학적 특성을 알아보기 위하여 각 유생 단계의 탈피율 및 탈피에 소요되는 시간을 알아보았다. 수온 20℃, 염도농도 17ppt의 조건에서 T. suecica를 먹이로서 충분량 급이 했을 때 P. nana의 어미로부터 갓 부화되어 나온 nauplius 유생은 성체가 되기까지 평균 17일이 소요됨을 알 수 있었다. 특히 제 1 nauplius 유생 단계에서 제 2 nauplius 유생으로 탈피하는 데에 평균 3.3일이 소요되어 가장 많은 시간을 요하는 것으로 나타났다. 점차 탈피하는데 소요되는 기간은 짧아져서 제 5 nauplius 유생 단계에서 제 6 nauplius 유생 단계로 탈피하는데에 가장 짧아서 약 1일이 소요되었고, 제 2 copepodid 유생 단계에서 제 3 copepodid 유생 단계로 탈피하는데 약 2.5일이 소요되었다. 한편, 제 1 nauplius 유생 단계에서 제 2 nauplius 유생 단계로의 탈피율은 66.7% 그리고, 제 6 nauplius 유생단계까지의 탈피율은 50%를 나타내었다. The moulting rate and inter-moult interval(days) of each larval developmental stages of Paracyclopina nana were studied. The copepod, P. nana was collected from lagoon Hwajinpo, South Korea. The experimental conditions were temperature 20℃, salinity l7ppt and fed on micro-algae, Tetraselmis suecica. The species was metamorphosed to the adult stage after possession of 6 nauplius stages and 5 copepodid stages. The moulting rate was 66.7% from 1st nauplius stage to 2nd one and was 50.0% to 6th one. P. nana were needed 17 days for developing from hatching to adult.
Establishment and Selection of Indicator Materials for Cervi Parvum Cornu Pharmacopuncture
Yang, Kyu-Jin,Lee, Ki-Beom,Kim, No-Hyeon,Kim, Tae-Gyu,Gi, Yu-Mi,Joo, Hwan-Soo,Suh, Chang-Yong,Lee, In-Hee,Chung, Hwa-Jin,Ha, In-Hyuk,Lee, Jae-Woong Korean AcupunctureMoxibustion Medicine Society 2017 Korean Journal of Acupuncture Vol.34 No.4
Background: Recently, Cervi Parvum Cornu pharmacopuncture has been widely used. But no studies on the indicator materials for Cervi Parvum Cornu pharmacopuncture have been conducted. The aim of this study was to select indicator materials that would aid in the uniform preparation of standardized Cervi Parvum Cornu pharmacopuncture. Methods: Three lots of Cervi Parvum Cornu pharmacopuncture were analysed. Each lot was prepared using the same methods and materials. Chondroitin sulfate, alanine, and leucine were selected as the indicator materials for Cervi Parvum Cornu. For standardization, chondroitin sulfate analysis was performed using the colorimetric method, while alanine and leucine were analyzed using liquid chromatography-mass spectrometry (LC-MS). Results: Analysis of the three lots of Cervi Parvum Cornu pharmacopuncture found chondroitin sulfate levels of $108.9{\pm}17.3ug/ml$, $118.8{\pm}5.0ug/ml$ and $112.3{\pm}11.9ug/ml$. Alanine levels were $44.9{\pm}2.8ug/ml$, $44.6{\pm}0.3ug/ml$, and $43.9{\pm}0.2ug/ml$. Leucine levels were $29.6{\pm}0.7ug/ml$, $29.0{\pm}0.1ug/ml$, and $29.4{\pm}0.1ug/ml$. Conclusion: These results suggest that chondroitin sulfate, alanine, and leucine may be useful for the standardization of Cervi Parvum Cornu pharmacopuncture.
Koo, Hwa Seon,Cha, Sun Hwa,Kim, Hye Ok,Song, In Ok,Min, Eung Gi,Yang, Kwang Moon,Park, Chan Woo The Korean Society for Reproductive Medicine 2015 Clinical and Experimental Reproductive Medicine Vol.42 No.4
Objective: The goal of this study was to investigate the relationship between serum progesterone (P4) levels on the day of human chorionic gonadotropin (hCG) administration and the pregnancy rate among women undergoing controlled ovarian stimulation for in vitro fertilization (IVF) or intracytoplasmic sperm injection-embryo transfer (ICSI-ET) using a flexible antagonist protocol. Methods: This prospective study included 200 IVF and ICSI-ET cycles in which a flexible antagonist protocol was used. The patients were divided into five distinct groups according to their serum P4 levels at the time of hCG administration (0.80, 0.85, 0.90, 0.95, and 1.00 ng/mL). The clinical pregnancy rate (CPR) was calculated for each P4 interval. Statistically significant differences were observed at a serum P4 level of 0.9 ng/mL. These data suggest that a serum P4 concentration of 0.9 ng/mL may represent the optimal threshold level for defining premature luteinization (PL) based on the presence of a significant negative impact on the CPR. Results: The CPR for each round of ET was significantly lower in the PL group defined using this threshold (25.8% vs. 41.8%; p=0.019), and the number of oocytes retrieved was significantly higher than in the non-PL group ($17.3{\pm}7.2$ vs. $11.0{\pm}7.2$; p=0.001). Elevated serum P4 levels on the day of hCG administration were associated with a reduced CPR, despite the retrieval of many oocytes. Conclusion: Measuring serum P4 values at the time of hCG administration is necessary in order to determine the optimal strategy for embryo transfer.
Shin, Gi Won,Cho, Yang Sook,Hwang, Hee Sung,Oh, Mi-Hwa,Nam, Hong Gil,Park, Jin Hyun,Jung, Gyoo Yeol WILEY-VCH Verlag 2009 Electrophoresis Vol.30 No.15
<P>Rapid diagnosis of bacterial infection is important for patient management and appropriate therapy during the early phase of bacteria-induced disease. Among the existing techniques for identifying microbial, CE-SSCP combined with 16S ribosomal RNA gene-specific PCR has the benefits of excellent sensitivity, resolution, and reproducibility. However, even though CE-SSCP can separate PCR products with high-resolution, multiplex detection and quantification are complicated by primer-dimer formation and non-specific amplification. Here, we describe a novel technique for multiplex detection and quantification of pathogens by template-tagging followed by multiplex asymmetric PCR and subsequent CE-SSCP. More specifically, we reverse transcribed 16S ribosomal RNAs from seven septicemia-inducing pathogens, tagged the templates with common end sequences, and amplified them using common primers. The resulting amplicons could be successfully separated by CE-SSCP and quantified by comparison to an internal standard. This method yielded results that illustrate the potential of this system for diagnosing infectious disease.</P>