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Yanfang Ren,Junyu He,Houyu Liu,Guoqing Liu,Xiaoling Ren 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.1
We investigated the effects of nitric oxide (NO) on quality, membrane lipid peroxidation, and antioxidantenzymes in mango (Mangifera indica L. cv ‘Tainong’) fruit during ripening. Fruits were treated with 0.25 mmol·L-1sodium nitroprusside (SNP) by the immersion method and stored at 23°C for 20 days. SNP treatment significantlysuppressed the respiration rate, enhanced fruit firmness, and decreased the rot index, peel color index, and weightloss in the fruit. This treatment also slowed the increase in soluble solids content (SSC) while maintaining highlevels of titratable acidity (TA), ascorbic acid (AsA), and phenolic compounds. Furthermore, SNP treatment enhancedthe antioxidant activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) and reduced theactivities of lipoxygenase (LOX) and polyphenol oxidase (PPO) in mango fruit, which was associated with the reducedaccumulation of malondialdehyde (MDA), superoxide anion radical (O2•-), and hydrogen peroxide (H2O2) comparedwith the control. Therefore, the addition of exogenous SNP has the potential for improving quality and prolongingthe shelf life of mango fruits by protecting them against oxidative damage caused by ROS during ripening.
Molecular detection and genetic diversity of bovine papillomavirus in dairy cows in Xinjiang, China
Qingling Meng,Chengcheng Ning,Lixia Wang,Yan Ren,Jie Li,Chencheng Xiao,Yanfang Li,Zhiyuan Li,Zhihao He,Xuepeng Cai,Jun Qiao 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.4
Background: Bovine papillomatosis is a type of proliferative tumor disease of skin and mucosae caused by bovine papillomavirus (BPV). As a transboundary and emerging disease in cattle, it poses a potential threat to the dairy industry. Objectives: The aim of this study is to detect and clarify the genetic diversity of BPV circulating in dairy cows in Xinjiang, China. Methods: 122 papilloma skin lesions from 8 intensive dairy farms located in different regions of Xinjiang, China were detected by polymerase chain reaction. The genetic evolution relationships of various types of BPVs were analyzed by examining this phylogenetic tree. Results: Ten genotypes of BPV (BPV1, BPV2, BPV3, BPV6, BPV7, BPV8, BPV10, BPV11, BPV13, and BPV14) were detected and identified in dairy cows. These were the first reported detections of BPV13 and BPV14 in Xinjiang, Mixed infections were detected, and there were geographical differences in the distribution of the BPV genotypes. Notably, the BPV infection rate among young cattle (< 1-year-old) developed from the same supply of frozen sperm was higher than that of the other young cows naturally raised under the same environmental conditions. Conclusions: Genotyping based on the L1 gene of BPV showed that BPVs circulating in Xinjiang China displayed substantial genetic diversity. This study provided valuable data at the molecular epidemiology level, which is conducive to developing deep insights into the genetic diversity and pathogenic characteristics of BPVs in dairy cows.