Encephalomyocarditis Virus 표면항원의 단일항체 생산세포주의 크론과 이의 면역학 및 생화학적 연구(Ⅱ)

양종대,박종수,이영탁,김화영,김영래,이인수,조영준,박재윤,차종희,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1

To see whether there is any differencies in RNA dependent DNA polymerase activities between monoclonal antibody-producing hydridoma cells and non-producing hybridoma cells, Balb/c female mice were immunized with the purified viral surface protein of D-variant of encephalomyocarditis virus and then fused with myeloma cells (NR-1). After cloring, monoclonal antibody-producing hybridoma cell lines were separated from non-producing hydridoma cell lines. RNA-dependent DNA polymerase activities were measured in the supernatant of monoclonal antibody-producing hybridoma clones and non-producing hybr idoma clones, and myeloma cells as control, Monoclonal antibody-producing hybridoma cells showed statistically significant higher activity as compar compare to that of nonproducing hybridoma cells. To find whether RNA-dependent DNA polymerase releasing cells aware secreting or budding C-type virus particles, those cells were examined with electron microscope. The hybridoma cell which secrete large amount of RNA-dependent DNA polymerase shows significant number of extracellular C-type virus particles. In constrast, non-producing hydridoma cells contains a lot of intracellular C-type virus particles. It is concluded that monoclonal antibody-producing hydridoma cells released particles. It is concluded that monoclonal antibody-producing hydridoma cells released significant amount of RNA-dependent DNA polymerase land extracellular C-type virus particles, while non-producing hydridoma cells showed less release of RNA-dependent DNA polymerase and contains intracellular C-type virus particles.

가족공동체를 떠난 간호대학생들의 대학생활체험

김고은,김유은,김희진,조유진,안주영,허다연,홍서우,차지영,박송이 이화여자대학교 간호학회 2018 이화간호학회지 Vol.- No.52

Purpose: The purpose of this study was to understand and explain the experiences of college life among junior nursing students who live apart from their families. Methods: 14 junior nursing students living apart from their families for at least one semester were recruited through snowballing and convenience sampling. Participants were interviewed individually using open ended questions. Data were collected from July to October in 2017 until data saturation. Interviews were transcribed verbatim and analyzed by using hermeneutic phenomenological method proposed by van Manen. Results: Five essential theme clusters emerged: (1) faced with diverse problems while living apart from family, (2) depend on family when stressed, (3) adapt to college life by being independent and forming self-initiative lifestyle, (4) confront with difficulties once again when adapting to academic curriculum restructured by practicum, (5) pave the way to nursing career and feel proud of overcoming the difficulties independently. Conclusion: Although most participants faced diverse problems in college life, they tried to adapt independently by forming self-initiative lifestyle and overturn the difficulties to positive experiences. In conclusion, the process of living apart from families transformed participants into proactive individuals. Adopting counseling programs could support starting their junior years successfully.

Development of Continuous Manufacturing Process for High-performance Thin Film Ni-YSZ Anode and Anode Functional Layer by Co-sputtering Method for Solid Oxide Fuel Cells

Y. J. Kim(김양재),S. Lee(이상훈),S. W. Cha(차석원) Korean Society for Precision Engineering 2021 한국정밀공학회 학술발표대회 논문집 Vol.2021 No.11월

The process of manufacturing an anode functional layer (AFL) in the conventional SOFC is inefficient, because, typically, the powder is ball-milled for a long time and sintered above 1,200°C. Also, in thin-film SOFCs, after fabricating the anode, a new target material or precursor is loaded on the vacuum deposition equipment to deposit the AFL. Alternatively, transferring the cell to other thin-film deposition equipment to lay the AFL requires more time and labor. Therefore, this study developed a process to fabricate an anode and AFL through the co-sputtering process continuously. By applying DC power to Ni and RF power to the Y/Zr metal alloy target, a Ni-YSZ anode of 700 nm and an AFL of 150 nm were continuously fabricated. This one-step fabrication process of an anode and AFL controls the proportion of the deposited material by adjusting DC and RF’s sputtering power. The film deposited with various power ratios was applied to anodic aluminum oxide wafers to characterize its electrochemical performance as SOFCs. XPS and EDS analyses were performed to confirm the compositional change according to the application of power. FESEM, AFM, and FIB-SEM analyses were performed to identify the microstructure change according to YSZ content.

Development of bulk heterojunction morphology by the difference of intermolecular interaction behaviors

Cha, H.,Baek, J.Y.,An, T.K.,Kim, S.O.,Kwon, S.K.,Kim, Y.H.,Park, C.E. Elsevier Science 2014 ORGANIC ELECTRONICS Vol.15 No.12

The morphology of a bulk heterojunction can be controlled by adding a processing additive in order to improve its power conversion efficiency (PCE) in photovoltaic devices. The phase-separated morphologies of blends of PONTBT or P3HT with fullerene derivatives are systematically examined in the presence of processing additives that possess various alkane alkyl chain lengths or end-group electronegativities. We determined the morphologies of the bulk heterojunction layers by using atomic force microscopy (AFM) and grazing incidence wide angle X-ray scattering (GIWAXS). The photocurrent-voltage characteristics of the bulk heterojunction solar cells were found to be strongly dependent on the intermolecular interactions between the conjugated polymers, the fullerene derivatives, and the processing additives in the photoactive layer. The optimal PONTBT:fullerene derivative blend morphology was obtained with a processing additive, 1,3-diiodopropane (1,3-DIP), that possesses a short alkyl chain and an end group with weak electronegativity, and was found to exhibit a high fill factor (FF) and a high current density (J<SUB>SC</SUB>). In contrast, in blends of P3HT with the fullerene derivative, PCEs with higher FF and J<SUB>SC</SUB> values were achieved by incorporating the processing additive, 1,8-dibromooctane (1,8-DBrO), which has a long alkyl chain and a strong electronegative end group. Thus the selection of the processing additive with the aim of enhancing photovoltaic performance needs to take into account the intermolecular interaction of the conjugated polymer.

Y염색체 장완 결실을 동반한 무정자증 1례

남윤성,김현주,이숙환,곽인평,윤태기,차광열,Nam, Y.S.,Kim, H.J.,Lee, S.H.,Kwak, I.P.,Yoon, T.K.,Cha, K.Y. 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.2

Different Y mutation in Yq11 occurring de novo in sterile males were first described 19 years ago. Since the phenotype of the patients was always associated with azoospermia or severe oligospermia, it was postulated that these mutations interrupt a Y spermatogenesis locus in the euchromatic Y region (Yq11) called azoospermia factor (AZF). Recently, it became possible to map AZF mutations to different subregions in Yq11by molecular deletion mapping. This indicated that azoospermia is possibly caused by more than one Y gene in Yq11 and the Yq11 chromatin structure. The frequency of AZF mutations in idiopathic sterile males $(5{\sim}20%)$ may indicate a need for a general screening programme for its analysis in infertility clinic. We have experienced a case of deletion distal to Yq11 region in azoospermic patient. So we report this case with a brief review of literatures.

운송하역 향상을 위한 Y/T 정차유도 프로토타입 장치 연구

김동훈(D. H. Kim),송준엽(J. Y. Song),차석근(S. K. Cha),강일용(I. Y. Kang),이승호(S. H. Lee) 한국정밀공학회 2009 한국정밀공학회 학술발표대회 논문집 Vol.2009 No.10월

흰쥐 적출 심근의 수축 현상에 대한 cyclobuxine과 칼슘 효현제인 Bay K 8644의 상호작용

박영현,김유재,김종배,차영덕,이종화,조병헌,권준택,김창호 순천향대학교 1992 논문집 Vol.15 No.2

Cyclobuxine, the major alkaloid of Buxus microphylla var. koreana Nakai, is a steroidal alkaloid recognized to contain a cyclopropane ring and has a substitution pattern at C-4 and C-14. In our previous studies, cyclobuxine blocked potassium-activated calcium channels(voltage-sensitive calcium channels) in ileal smooth muscle. Cyclobuxine also exerted negative chronotropic and inotropic effects in the anesthetized rat and the isolated rat heart. In this study, antagonism between either the dihydropyridine calcium agonist, Bay K 8644, and high external Ca++ or nifedipine, verapamil, diltiazem, and cyclobuxine was investigated in the isolated ventricular muscle from rats. Bay K 8644(?? M) produced a slowly developing increase in developed tension. Cyclobuxine and the calcium antagonists decreased dose-dependently developed tension. Bay K 8644(??M) produced a rightward parallel shift of the concentration-response curves for the negative inotropic effects of nifedipine(??). Bay K 8644 produced only a slight rightward shift of the concentration-response curves for the negative inotropic effects of diltiazem(??) and cyclobuxine (??). These results indicate that Bay K 8644 antagonizes the negative inotropic effect of nifedipine by competing with the same site closely associated with the calcium channel. In contrast, Bay K 8644 antagonizes noncompetitively the negative inotropic effects of cyclobuxine, verapamil and diltiazem in the isolated ventricular muscle.

노인의 약물 지식 정도와 약물 사용실태 및 약물 오·남용 행위

구예나,김민경,김진윤,배윤,서민혜,정은혜,지수현,차미나,Choi, Jenny 이화여자대학교 간호과학대학 2013 이화간호학회지 Vol.- No.47

Purpose: The purpose of this study was to provide basic data for use in the upgrade of healthcare for the elderly and to provide guidance toward educating the elderly in the proper use of medications by determining the level of drug knowledge, current use, and adherence by the elderly. Method: The participants of this study were selected using the convenient sampling method. Participants of this study comprised of 160 elderly who were aged 65 or older, who were participants to the elderly municipal welfare center in Seoul. The instrument used for this study was a structured questionnaire (Han,2011). Collected data were analyzed using the SPSS20.0 program with descriptive statistics, t-test, ANOVA, and Pearson’s correlation coefficient. Result: The mean score for drug knowledge was 7.6±2.9 and drug misuse was 4.7±2.8. There were significant differences about drug knowledge according to the following: level of education (F=8.053 p=.000), and spouse (t=2.14 p=.034). There were significant differences between drug misuse and monthly income (F=2.167 p=.040). There was no statistically significant relationship between drug knowledge and drug misuse (r=-.087, p=.271). Conclusion: A replication study based on broader region and in-depth research is needed. The development of the customized education protocol considering various levels of education, income, spouse and physical condition are needed. Further research is suggested to find out the status of medication education for the elderly by nurses.

MSV로 변이시킨 가토신장세포의 배양조건에 따른 지방조성변화에 대한 연구

최한용,박주홍,김화영,김영래,이인수,조영준,박재윤,차종희,고광삼 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1

Differencies of lipid composition of murine sarcoma virus 97017 transformed rabbit kidney cells and their plasma membranes in monolayer and suspension culture were studied. Free cholesterol was the major neutral lipid extracted from the whole cells and plasma membranes of all these cultures. Triglyceride increased about 6 to 50 folds in suspension grown whole cells and their plasma membranes compared to those from monolayer cultures. The μ mole of total neutral lipids and phospholipids are elevated in plasma membranes compared to whole cells from monolayer cultures bur arc similar in suspension cultures. The distribution of fatty acids in the major lipid classes of plasma membranes and whole cells generally follow the composition of calf serumused in the culture media.

중년남성의 지각된 건강상태, 가족응집력 및 건강증진행위에 관한 연구

김지선,노원영,박새별,유동민,이가은,이주애,전유라,조세영,차지영,고지현 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

health promotion behavior of middle aged men and the relationship among the above factors. Methods: Subjects of the research were 325 middle aged men in Korea. The questionnaires were composed of general characteristics, Health Self Rating Scale, FACES-Ⅲ : Family Adaptability & Cohesion Evaluation Scale Ⅲ, Health Promoting Lifestyle Profile. Data were statistically analyzed by t-test, ANOVA, Scheffe test and Pearson’s correlation. Results: Middle aged men whose perceived economic status are low show lower perceived health status than moderate and high (F=3.364, p=.010). Health promotion behavior in the age 40-44 shows lower level than 60-64 (F=2.984, p=.019). Middle aged men who have Economically dependent adult in the first child show higher level in health promotion behavior than who have Middle & High school student in the first child (F=2.468, p=.045). In the middle aged men, perceived health status and family cohesion show positive correlation (r=.341, p<.01). Perceived health status and health promotion behavior show positive correlation (r=.500, p<.01). Family cohesion and health promotion behavior show posotive correlation (r=.564, p<.01). Conclusions: We analyzed influencing factor and a relationship between perceived health status, family cohesion and health promotion behavior. Accordingly, we can think of applying nursing interventions to the middle aged men considering the general characteristics. Also we can think of applying nursing interventions and educations to help them evaluate their perceived health status and include the support of family members for the health promotion behavior.