Comparative Study on Transcatheter Arterial Chemoembolization, Portal Vein Embolization and High Intensity Focused Ultrasound Sequential Therapy for Patients

Cui, Lin,Liu, Xing-Xiang,Jiang, Yong,Wu, Xing-Jun,Liu, Jian-Jun,Zhou, Xiang-Rong,He, Xue-Jun,Huang, Xin-En Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Objective: To investigate the safety and efficacy of transcatheter arterial chemoembolization (TACE), combined with portal vein embolization (PVE), and high intensity focused ultrasound (HIFU) sequential therapy in treating patients with hepatocellular carcinoma (HCC). Methods: Patients with inoperative HCC were treated by two methods: in the study group with TACE first, then PVE a week later, and then TACE+PVE every two months as a cycle, after 2~3 cycles finally HIFU was given; in the control group only TACE+PVE was given. Response (CR+PR), and disease control rate (CR+PR+SD), side effects, overall survival and time to progress were calculated. Results: Main side effects of both groups were nausea and vomiting. No treatment related death occurred. In the study group, 32 patients received TACE for overall 67 times, PVE 64 times, and HIFU 99 times; on average 2.1, 2 and 3.1 times for each patient, respectively. In the control group, 36 patients were given TACE 78 times and PVE 74 times, averaging 2.2 and 2.1 times per patient. Effective rate: 25.0% in study group and 8.3% in control group (p>0.05). Disease control rates were 71.9% and 44.4%, respectively (p<0.05). In patients with portal vein tumor thrombus, the rate reduced over 1/2 after treatment was 69.2%(9/13) in the study and 21.4%(3/14) in the control group (p<0.05). Rate of AFP reversion or decrease over 1/2 was 66.7%(16/24) in study and 37%(10/27) (p<0.05) in control group. Median survival time: 16 months in study and 10 months in control group. PFS was 7months in study and 3 months in control group. Log-rank test suggested that statistically significant difference exists between two groups (p=0.024). 1-, 2- and 3-year survival rates were 56.3%, 18.8% and 9.3% in study, while 30.6%, 5.6% and 0 in control group, respectively, with statistically significant difference between two groups (by Log-rank, p = 0.014). Conclusions: The treatment of TACE+PVE+HIFU sequential therapy for HCC increases response rate, prolong survival, and could thus be a safe and effective treatment for advanced cases.

Evaluation of Biogas Production Performance and Dynamics of the Microbial Community in Different Straws

( Xue Li ),( Yan-hua Liu ),( Xin Zhang ),( Chang-ming Ge ),( Ren-zhe Piao ),( Wei-dong Wang ),( Zong-jun Cui ),( Hong-yan Zhao ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3

The development and utilization of crop straw biogas resources can effectively alleviate the shortage of energy, environmental pollution, and other issues. This study performed a continuous batch test at 35°C to assess the methane production potential and volatile organic acid contents using the modified Gompertz equation. Illumina MiSeq platform sequencing, which is a sequencing method based on sequencing-by-synthesis, was used to compare the archaeal community diversity, and denaturing gradient gel electrophoresis (DGGE) was used to analyze the bacterial community diversity in rice straw, dry maize straw, silage maize straw, and tobacco straw. The results showed that cumulative gas production values for silage maize straw, rice straw, dry maize straw, and tobacco straw were 4,870, 4,032.5, 3,907.5, and 3,628.3 ml/g ·VS , respectively, after 24 days. Maximum daily gas production values of silage maize straw and rice straw were 1,025 and 904.17 ml/g ·VS, respectively, followed by tobacco straw and dry maize straw. The methane content of all four kinds of straws was > 60%, particularly that of silage maize straw, which peaked at 67.3%. Biogas production from the four kinds of straw was in the order silage maize straw > rice straw > dry maize straw > tobacco straw, and the values were 1,166.7, 1,048.4, 890, and 637.4 ml/g ·VS, respectively. The microbial community analysis showed that metabolism was mainly carried out by acetateutilizing methanogens, and that Methanosarcina was the dominant archaeal genus in the four kinds of straw, and the DGGE bands belonged to the phyla Firmicutes, Bacteroidetes, and Chloroflexi. Silage maize is useful for biogas production because it contains four kinds of straw.

Molecular characterization and ligand-binding properties of six odorantbinding proteins (OBPs) from Aphis gossypii

Xue-Ke Gao,Shuai Zhang,Jun-Yu Luo,Chun-Yi Wang,Li-Min Lü,Li-Juan Zhang,Xiang-Zhen Zhu,Li Wang,Jin-Jie Cui 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.3

Odorant-binding proteins (OBPs) play a significant role in the olfactory signal transduction of insects and help them locate hosts, oviposition sites, and mating partners. The sap-sucking insect, Aphis gossypii Glover (Homoptera: Aphididae), is a destructive cosmopolitan pest and yet the molecular mechanisms by which A. gossypii perceives pheromones and host volatiles remain unknown. In this study, we identified and characterized 6 OBPs, using the A. gossypii RNA-seq transcriptome dataset previously constructed in our laboratory. Real-time PCR indicated specific expression patterns of the 6 genes, which had different levels of expression based on development stage, tissue, morph, and life cycle. In addition, binding specificities of the 6 proteins investigated using the ligand-binding assays showed that all 6 OBPs exhibited high binding affinities towards Phlorizin dehydrate while AgosOBP3 and AgosOBP4 had strong affinity to beta-ionone and AgosOBP8 displayed higher binding affinities for Nerolidol and Cis-3-hexenyl acetate compared to other OBPs.

A highly active Z-scheme NiGa2O4/anthraquinone/MoO3 photocatalyst via charge transfer for sunlight photocatalytic simultaneous conversions of nitrite and sulfite

Xue Ma,Siyi Li,Zhihui Qu,Meng Zhang,Jing Qiao,Xin Cui,Chunquan Wang,Jun Wang,Youtao Song 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.78 No.-

The separation efficiency of photogenerated electron-hole pairs (e –h+) is an important factor for ahighly photocatalytic activity of Z-scheme photocatalytic system. In order to suppress the recombining ofphotogenerated e and h+ and enhance the electron transfer rate, anthraquinone (AQ) as special electrontransfer channel is combined with NiGa2O4 and MoO3, forming a Z-scheme NiGa2O4/AQ/MoO3photocatalyst. Due to unique chemical and physical properties, AQ provides a novel electron transmissionway. Compared with traditional conductive channels (for example, noble metals and graphene), in thisway, the AQ may greatly enhance the electron transfer efficiency. In addition, the related morphology,optical properties and chemical composition were investigated by a sequence of characterizationmethods. The influences of photocatalyst species, irradiation time and used times in simultaneouslyconverting nitrite and sulfite under simulated sunlight were also studied. The results indicate that theprepared Z-scheme NiGa2O4/AQ/MoO3 photocatalyst exhibits a much high activity. The conversion ratesof nitrite and sulfite can reach 89.81% and 94.47%, respectively, under simulated sunlight irradiation forfour hours. The enhanced photocatalytic activity is attributed to the fact that AQ can rapidly transferelectron by inner charge transfer. This charge transfer way can provide a new idea to design subsequentphotocatalysts.

Phase II Study on Dose Escalating Schedule of Paclitaxel Concurrent with Radiotherapy in Treating Patients with Locally Advanced Non-small Cell Lung Cancer

Cui, Lin,Liu, Xing-Xiang,Jiang, Yong,Liu, Jian-Jun,Zhou, Xiang-Rong,He, Xue-Jun,Chen, Jue,Huang, Xin-En Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Objective: To evaluate clinical efficacy of a dose escalating schedule of paclitaxel concurrent with radiotherapy in treating patients with locally advanced non-small cell lung (NSCLC). Methods: Patients with locally advanced NSCLC were treated with conventional fractionated radiotherapy or three dimensional conformal radiotherapy (3 DCRT), concurrently with a dose escalating schedule of paclitaxel. All patients were divided into three groups, A with paclitaxel $30mg/m^2$, B with paclitaxel $60mg/m^2$ and C with paclitaxel $90mg/m^2$. Paclitaxel was repeated every week for a total of 4 or 6 weeks. Results: Among 109 patients, response rates were 68.8%, 71.1% and 71.8% (p>0.05) for group A (n=32), B (n=38), and C (n=39) respectively. Accordingly, disease control rates were 81.3%, 81.6% and 82.1% (p>0.05). Progression-free survival time was $8.0{\pm}5.0$ months, $11.6{\pm}6.1$ months, and $14.8{\pm}7.9$ months (p<0.05), respectively. Overall survival time was $15.4{\pm}7.6$ months, $18.2{\pm}8.0$ months, and $22.0{\pm}7.6$ months (p<0.05), one-year survival rates were 62.5%, 73.1% and 90.0% (p>0.05) and two-year survival rates were 31.3%, 38.5% and 50.0% (p<0.05). Main side-effects were bone marrow suppression, radiation related esophagitis and gastrointestinal reaction. Conclusion: In treating patients with NSCLC, concurrent chemoradiotherapy with paclitaxel improves early response compared with conventional fractionated radiotherapy or 3 DCRT. The survival rate was improved with the addition of paclitaxel, but there was an increase in adverse reactions when the dose of paclitaxel was increased.

Silencing of the COPS3 Gene by siRNA Reduces Proliferation of Lung Cancer Cells Most Likely via induction of Cell Cycle Arrest and Apoptosis

Wang, Xue-Mei,Cui, Jiu-Wei,Li, Wei,Cai, Lu,Song, Wei,Wang, Guan-Jun Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

The COPS3 gene has stimulating effect on cell proliferation and progression of osteosarcomas and related cells. However, the features of COPS3 and its potential application as a therapeutic target in other cancers has not yet been studied. In this study, therefore, the effect of COPS3 silencing via COPS3 siRNA on lung cancer cell proliferation was examined. Expression levels of COPS3 gene in COPS3 siRNA infected cells and control siRNA infected cells were compared with real time PCR and Western blot analysis. Cell proliferation levels were comprehensively analyzed by MTT, BrdU incorporationy, and colony formation assays. For mechanistic assessment the effects of COPS3 silencing on cell cycle and apoptosis were analyzed using flow cytometry. Results showed that successful silencing of the COPS3 gene at both translational and transcriptional levels significantly reduced the proliferation and colony formation by lung cancer cells (p<0.01). Flow cytometry showed cell cycle arrest in the G0/G1 phase after COPS3 silencing, and more importantly, apoptosis was induced as a result of COPS3 knockdown, which negatively affected cell survival. Therefore, these results provide another piece of important evidence that the COPS3 gene expressed in lung cancer cells may play a critical role in stimulating proliferation. Down-regulation of COPS3 could significantly inhibit lung cancer cell growth, which was most likely mediated via induction of cell cycle arrest in G0/G1 phase and apoptosis.

멀티프로세서 기반의 고속 영상처리 기술에 대한 벤치마킹

최학남(Xue-Nan Cui),박은수(Eun-Soo Park),김준철(Jun-Chul Kim),김학일(Hak-Il Kim) 대한전기학회 2007 대한전기학회 학술대회 논문집 Vol.2007 No.10

본 논문에서는 멀티프로세서 기반의 고속 영상처리 알고리즘 개발방법에 대해 소개한다. 영상획득 방식의 발전과 더불어 고해상도 영상의 획득이 가능해지고 영상이 컬러화가 되면서 많은 영상처리 응용분야에서 알고리즘 고속화를 필요로 하고 있다. 이러한 수요를 만족시키기 위해서는 최근에 출시되고 있는 멀티프로세서를 최대한 활용할 수 있는 알고리즘 개발이 최우선이다. 본 논문에서는 OpeMP, MIL(Matrox Image Library), OpenCV, IPP(Integrated Performance Primitives), SSE (Streaming SIMD (Single Instruction Multiple Data) Extensions)등 병렬처리와 고속 영상처리 라이브러리를 이용한 알고리즘 개발방법에 대해 소개하고, 각 개발방법에 따른 알고리즘 성능을 분석 및 평가하였다. 실험결과로부터 SSE와 IPP, MIL(Thread)을 이용하여 Mean, Dilation, Erosion, Open, Closing, Sobel등 알고리즘을 구현하여 4057 × 4048크기의 영상에 적용하였을 때 7~35msec의 좋은 성능을 나타내어 기타 방식보다 우수함을 알 수 있었다.

GPU를 이용한 Gabor Texture 특징점 기반의 금속 패드 변색 분류 알고리즘

최학남(Xue-Nan Cui),박은수(Eun-soo Park),김준철(Jun-Chul Kim),김학일(Hakil Kim) 제어로봇시스템학회 2009 제어·로봇·시스템학회 논문지 Vol.15 No.8

This paper presents a Gabor texture feature extraction method for classification of discolored Metal pad images using GPU(Graphics Processing Unit). The proposed algorithm extracts the texture information using Gabor filters and constructs a pattern map using the extracted information. Finally, the golden pad images are classified by utilizing the feature vectors which are extracted from the constructed pattern map. Tn order to evaluate the performance of the Gabor texture feature extraction algorithm based on GPU, a sequential processing and parallel processing using Open MP in GPU of this algorithm were adopted. Also, the proposed algorithm was implemented by using Global memory and Shared memory in GPU. The experimental results were demonstrated that the method using Shared memory in GPU provides the best performance. For evaluating the effectiveness of extracted Gabor texture features, an experimental validation has been conducted on a database of 20 Metal pad images and the experiment has shown no mis-classification.

잡음과 회전에 강인한 SIFT 기반 PCB 영상 정렬 알고리즘 개발

김준철(Jun-Chul Kim),최학남(Xue-nan Cui),박은수(Eun-Soo Park),최효훈(Hyo-hoon Choi),김학일(Hakil Kim) 제어로봇시스템학회 2010 제어·로봇·시스템학회 논문지 Vol.16 No.7

This paper presents an image alignment algorithm for application of AOI (Automatic Optical Inspection) based on SIFT. Since the correspondences result using SIFT descriptor have many wrong points for aligning, this paper modified and classified those points by five measures called the CCFMR (Cascade Classifier for False Matching Reduction) After reduced the false matching, rotation and translation are estimated by point selection method. Experimental results show that the proposed method has fewer fail matching in comparison to commercial software MIL 8.0, and specially, less than twice with the well-controlled environment’s data sets (such as AOI system). The rotation and translation accuracy is robust than MIL in the noise data sets, but the errors are higher than in a rotation variation data sets although that also meaningful result in the practical system. In addition to, the computational time consumed by the proposed method is four times shorter than that by MIL which increases linearly according to noise.

Population dynamics, hunting nature on insect pests and existence of symbiotic bacterial microbes among leading transgenic cotton spiders

Nasir Muhammad,Zhao Chen-chen,Luo Jun-yu,Zhu Xiang-zhen,Gao Xue-ke,Iqbal Mazher F.,Ji Ji-chao,Zhang Shuai,Cui Jin-jie 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1

The genetically modified cotton holding Bt proteins, is noxious to bollworms larvae but very little is known about its impact on population of non-target spiders within the field. Studies conducted with the aim to identify spiders along with their abundance, prevalence pattern, preying habit on insect pests in Bt cotton (L280, Cry2Ab4) and commonness of symbiotic bacteria in these spiders. Spiders collections were made via vial tapping/jarring, from May to end of October (2018). In total, 13,342 spiders were recorded during the entire cropping season. Least relative abundance was in May (0.28%), highest in August (30.39%) while ahead decline was observed, reached to 12% in October. The 27 species were verified, least richness was in May (3 species), high in August (19 species), later in the end, reduced to 7 species. High abundance was of Hylyphantes graminicola (68.56%) and Neoscona theisi (19.98%). Existence of H. graminicola and Pardosa astrigera observed during the whole cropping season. Presence of seven guilds among spiders had a diverse nature of hunting insect pests and on number of species existed in the crop, maximum was from direct hunting habit. Composition and community structure of microbiota varied as of spider species. Proteobacteria was the most abundant bacterial phylum and topmost genus was Wolbachia in observed spiders. Five groups of bacteria distinguished across 4 clusters of spiders depending on core community of symbiotic bacterial genera. This effort is an initial step to get knowhow about spiders in Bt cotton, which will facilitate future research studies concerning spiders.