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Huang, Jinming,You, Wei,Wu, Naike,Tan, Xiuwen Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.6
Testis-specific protein (TSPY) is a Y-specific gene, with up to 200 copy numbers in bulls. In order to make bovine embryo sexing under farm condition more feasible, the possibility of using a non-electrophoretic method to detect the TSPY gene for sexing bovine early embryos was examined. Primers were designed to amplify a portion of the TSPY gene and a common gene as an internal control primer. PCR optimization was carried out using a DNA template from bovine whole blood. Furthermore, embryo samples were diagnosed by this method and the sexing results were contrasted with those of the Loop-Mediated Isothermal Amplification (LAMP) method. The results showed that TSPY was as reliable a sexing method as LAMP. Forty-three morula and blastocyst embryos collected from superovulated donor dairy cattle were sexed by this method, and twenty-one embryos judged to be female embryos were transferred non-surgically to recipients 6 to 8 days after natural estrus. Out of 21 recipients, 9 were pregnant (42.86%) and all delivered female calves. The results showed that the sex predicted by this protocol was 100% accurate. In conclusion, the TSPY gene was a good male specific marker and indicated that a non-electrophoretic method was feasible and accurate to detect the TSPY gene for sexing preimplantation bovine embryos.
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Qi Zhao,Xiujuan Yu,Xiuwen Cheng,Juan Wu 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.6
Sulfur-free exfoliated graphite (EG) with large exfoliated volume (760 mL g-1) was prepared throughchemical oxidation, followed by microwave exfoliation. SEM results indicated that well-developednetwork pores in the worm-like structure of EG could be clearly observed. XPS results displayed thatsome functional groups were yielded on the surface of the as-prepared EG. Moreover, the capacity of EGwas evaluated by the adsorption of rodamine B and 4-chlorophenol aqueous solution. Results showedthat EG exhibited high adsorption capacity for RhB and 4-CP, which could be attributed to the functionalgroups and well-developed network pores in the worm-like EG.
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Cheng Zhao,Qiongyuan Hu,Xiuwen Wu,Guopu Chen,Hua Wu,Jianan Ren 한국고분자학회 2019 Macromolecular Research Vol.27 No.9
Previous studies have established that sole probiotics or fibre could be used to relieve colitis. However, few studies evaluated the synergistical effect of fibre and probiotics in treating colitis. This study, examined the effect of alginate, one of the commonest biomaterials and dietary fibre, and/or probiotics on the gut barrier in colitis mice. A synergistical effect was found between alginate and probiotics in colitis. Dextran sulfate sodium salt (DSS), a chemical toxic substance, was used to induce colitis model. Body weight, oxidative stress, inflammatory factors, gut barrier, intestinal morphology and the apoptosis of intestine epithelial cells were analyzed. A combination of probiotics and alginate was used to analyze their synergistic effect. The results showed that the combination of alginate and probiotics had a better therapeutic effect on colitis than using them individually, and they protected the gut barrier from DSS induced oxidative stress. The combination exhibited a greater ability in reducing pro-inflammatory factors and protecting the gut barrier from further damage. The synergistic effect of alginate and probiotics provides a great prospect for biotherapy in bowel diseases and oral drug delivery system.
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Recent Advances in Functional Hydrogel for Repair of Abdominal Wall Defects: A Review
Ye Liu,Jinjian Huang,Sicheng Li,Ze Li,Canwen Chen,Guiwen Qu,Kang Chen,Yitian Teng,Rui Ma,Jianan Ren,Xiuwen Wu 한국생체재료학회 2024 생체재료학회지 Vol.28 No.00
The abdominal wall plays a crucial role in safeguarding the internal organs of the body, serving as an essential protective barrier. Defects in the abdominal wall are common due to surgery, infection, or trauma. Complex defects have limited self-healing capacity and require external intervention. Traditional treatments have drawbacks, and biomaterials have not fully achieved the desired outcomes. Hydrogel has emerged as a promising strategy that is extensively studied and applied in promoting tissue regeneration by filling or repairing damaged tissue due to its unique properties. This review summarizes the five prominent properties and advances in using hydrogels to enhance the healing and repair of abdominal wall defects: (a) good biocompatibility with host tissues that reduces adverse reactions and immune responses while supporting cell adhesion migration proliferation; (b) tunable mechanical properties matching those of the abdominal wall that adapt to normal movement deformations while reducing tissue stress, thereby influencing regulating cell behavior tissue regeneration; (c) drug carriers continuously delivering drugs and bioactive molecules to sites optimizing healing processes enhancing tissue regeneration; (d) promotion of cell interactions by simulating hydrated extracellular matrix environments, providing physical support, space, and cues for cell migration, adhesion, and proliferation; (e) easy manipulation and application in surgical procedures, allowing precise placement and close adhesion to the defective abdominal wall, providing mechanical support. Additionally, the advances of hydrogels for repairing defects in the abdominal wall are also mentioned. Finally, an overview is provided on the current obstacles and constraints faced by hydrogels, along with potential prospects in the repair of abdominal wall defects.
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