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- 저자 : Xiujuan Qi (검색결과 4 건)
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Xiujuan Tian,Wenjing Qi,Hongyu Chen,Xianlu Zeng,Liping Han,Donghui Mi 한국통합생물학회 2016 Animal cells and systems Vol.20 No.5
In pre-initiation complexes, RNA helicase A interacts with β-actin and acts as a bridging factor linking nuclear actin with RNA polymerase II (Pol II). In addition, β-actin participates in Pol IIdependent transcription elongation by interacting with the positive transcription elongation factor Cdk9. However, many relationships between β-actin and Pol II remain to be identified. In an interleukin 6 (IL-6)-induced p21 expression model, we demonstrated that β-actin knockdown reduced p21 expression. Immunofluorescence analysis showed that the colocalization of β-actin and Pol II increased significantly in cells treated with IL-6. It is known that the Rpb5, Rpb6 and Rpb7 subunits are located at the surface of the enzyme. We next constructed recombinant pcDNA-HA-Rpb5, pcDNA-HA-Rpb6 and pcDNA-HA-Rpb7 plasmids and expressed the three polymerase II subunits in HepG2 cells. We found that β-actin could be immunoprecipitated with HA-Rpb5 and HA-Rpb7. A Glutathione-S-transferase pull-down assay revealed that β-actin was associated with Rpb5 and Rpb7 in vitro. Furthermore, overexpression of Rpb5 and Rpb7 in cells reduced p21 expression significantly, suggesting that Rpb5 and Rpb7 competitively interact with β-actin. This study shows that β-actin associates with Pol II subunits through direct proteinprotein interactions and provides fundamental insight into Pol II transcriptional regulation.
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Qi Zhao,Xiujuan Yu,Xiuwen Cheng,Juan Wu 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.6
Sulfur-free exfoliated graphite (EG) with large exfoliated volume (760 mL g-1) was prepared throughchemical oxidation, followed by microwave exfoliation. SEM results indicated that well-developednetwork pores in the worm-like structure of EG could be clearly observed. XPS results displayed thatsome functional groups were yielded on the surface of the as-prepared EG. Moreover, the capacity of EGwas evaluated by the adsorption of rodamine B and 4-chlorophenol aqueous solution. Results showedthat EG exhibited high adsorption capacity for RhB and 4-CP, which could be attributed to the functionalgroups and well-developed network pores in the worm-like EG.
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Evaluation of freezing tolerance in Actinidia germplasm based on relative electrolyte leakage
Shihang Sun,Xiujuan Qi,Ran Wang,Miaomiao Lin,Jinbao Fang 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.4
Cold stress in winter can have a disastrous eff ect on kiwifruit yield and aff ect geographical distribution. However, freezingtolerance in Actinidia genotypes remains largely unknown. Here, we report changes in metabolite content and enzymeactivity in the shoots of Actinidia genotypes exposed to low-temperature stress (− 5 °C, − 10 °C, − 15 °C, − 20 °C, − 25 °Cand − 30 °C). Moreover, the relative electrolyte leakage method was used to evaluate the freezing tolerance of kiwifruitgermplasm; 51 genotypes from 16 species of Actinidia were evaluated in total. The data revealed that relative electrolyteleakage, proline (Pro), soluble protein, and catalase (CAT) activity changed with diff erent low temperatures. Results showedthat among 16 species, A. kolomikta , A. polygama , and A. arguta had lower LT50 than other species. A. arguta , originatingfrom the northeast of China, exhibited stronger freezing resistance than the ones from other places. There was little diff erencein freezing tolerance between A. chinensis and A. deliciosa . These fi ndings provide new insights into the freezing toleranceability and mechanisms of kiwifruit and further contribute to our understanding of the relationship between freezing toleranceand geographic distribution.
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Zhi Li,Yunpeng Zhong,Danfeng Bai,Miaomiao Lin,Xiujuan Qi,Jinbao Fang 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.5
Kiwifruit ( Actinidia sp.) vines are poorly adapted to waterlogging stress. Actinidia valvata is more tolerant against waterloggingstress than Actinidia deliciosa , and the latter contains some common rootstocks that are frequently used in kiwifruit cultivation. Little is known about the responses of A. valvata genotypes against waterlogging stress and during post-waterloggingrecovery. Here, we compared physiological traits of three A. valvata genotypes (KR1, KR3, and KR5) during waterloggingstress and recovery. Kiwifruit vines displayed water loss, a decline in the net photosynthetic rate, and inhibited shoot elongationduring waterlogging. These three genotypes could endure long-term waterlogging owing to their unique root systemconfi gurations as well as by sustaining carbohydrate reserves in the roots. Feeder roots of KR1 vines were damaged earlierand lost water more quickly than the other genotypes. Under the same stress, KR3 formed adventitious roots more rapidly,while KR5 had an improved ability to control water loss in above-ground tissues. After reoxygenation, growth of vineswas partially recovered due to water loss control, photosynthetic recovery, and carbohydrate replenishment. KR3 and KR5recovered their growth earlier and replenished more carbohydrates than KR1 after re-aeration. During waterlogging, both therelative water content and carbohydrate levels of vines can limit the recovery effi ciency after re-aeration. Our results revealedmutual and distinct responses of diff erent A. valvata genotypes during waterlogging stress and recovery and provided moreinsight into the physiological basis of their adaptation to waterlogging stress.
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