In Vitro Screening for Compounds Derived from Traditional Chinese Medicines with Antiviral Activities Against Porcine Reproductive and Respiratory Syndrome Virus

( Jia Cheng ),( Na Sun ),( Xin Zhao ),( Li Niu ),( Mei Qin Song ),( Yao Gui Sun ),( Jun Bing Jiang ),( Jian Hua Guo2 ),( Yuan Sheng Bai ),( Jun Ping He ),( Hong Quan Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8

Seventeen compounds derived from traditional Chinese medicines (TCMs) were tested for their antiviral activity against porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. Visualization with the cytopathologic effect (CPE) assay and the 3-(4, 5-dimethyithiazol- 2-yl)-2,5-diphenyltetrazolium bromide test were used to determine the 50% cytotoxic concentration (CC50) and 50% effective concentration (EC50) in cultured Marc-145 cells. Among the tested compounds, chlorogenic acid and scutellarin showed potential anti-PRRSV activity. The EC50 values were 270.8 ± 14.6 μg/ml and 28.21 ± 26.0 μg/ml and the selectivity indexes were >5.54 and 35.5, respectively. The time-of-addition and virucidal assay indicated that the anti-PRRSV activity of the two compounds could be due to their inhibiting the early stage of virus replication and/or inactivating the virus directly. The inhibition of the virus attachment was not observed in the adsorption inhibition assay. The inhibition ratios of chlorogenic acid and scutellarin were, respectively, 90.8% and 61.1% at the maximum non-cytotoxic concentrations. The results have provided a basis for further exploration of their antiviral properties and mechanisms in vivo. We believe that the chlorogenic acid and scutellarin have a great potential to be developed as new anti-PRRSV drugs for clinical application.

Analysis of Factors Influencing Communication Popularity Based on Short Video Information Sources: Audience Trust as a Mediated Research Review

Xin Guo(Guo Xin ),Fang Deng(Deng Fang ) 동북아경상학회 2022 동북아경상연구 Vol.3 No.1

Purpose – In the online environment, the group definition, marginal scope, and transmission methods of short video creators and audiences have all changed, but the centrality of the audiences’ essence will not change. Their thinking characteristics and behaviors ultimately determine the influence of digital products spread in the network. This paper attempts to explore the relationship between audience trust and communication mechanism from the source of information: the trust or suspicion generated by the creator’s identity type information, and explore its influence on the popularity of communication. Design/Methodology/Approach – We systematically reviews the connotation and extension of audience trust in online media, definition and characteristics of network identity, the disclosure of creator identity types, character setting, authentication and supervision of identity, and communication popularity. Findings – The interaction between short video media and audience includes: information dissemination, value judgment and emotional transmission. Audience trust plays a bridge and check position in the dissemination of short video media, which determines the survival and development of short video media. These contents can provide short video creators with basic information that can effectively affect user behavior, provide them with a basis for decisionmaking in the creation process, increase audience trust, and improve the popularity and effectiveness of media communication. Research Implications – It provides a new research perspective for influencing user identification, social recommendation and other fields.

The effect of cyfluthrin on testis inhibin B in rats and the intervention of Lycium barbarum polysaccharide

Guo Xin,Xie Yong-Xin,Guo Chen,Wei Jing-Lin,Yang Hui-Fang 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.2

Background In recent years, studies have shown that cyfluthrin is harmful to the reproductive system. Lycium barbarum polysaccharide may be a protective agent. Objective To investigate the effect of cyfluthrin (Cy) on testosterone B (inhibin B, INHB) in male rats and the intervention of Lycium barbarum polysaccharide (LBP). Results Compared with the control group, the mRNA and protein expression levels of INH α and INH βB in the testis tissue decreased, and the difference was statistically significant (P < 0.05). The LBP intervention group can significantly increase the expression level, and the difference is statistically significant (P < 0.05). Conclusion The decrease of INHα and INHβB expression caused by Cy exposure indicates that Cy has a toxic effect on the testis of rats, and the addition of LBP has a certain protective effect on the testis of rats. Background In recent years, studies have shown that cyfluthrin is harmful to the reproductive system. Lycium barbarum polysaccharide may be a protective agent. Objective To investigate the effect of cyfluthrin (Cy) on testosterone B (inhibin B, INHB) in male rats and the intervention of Lycium barbarum polysaccharide (LBP). Results Compared with the control group, the mRNA and protein expression levels of INH α and INH βB in the testis tissue decreased, and the difference was statistically significant (P < 0.05). The LBP intervention group can significantly increase the expression level, and the difference is statistically significant (P < 0.05). Conclusion The decrease of INHα and INHβB expression caused by Cy exposure indicates that Cy has a toxic effect on the testis of rats, and the addition of LBP has a certain protective effect on the testis of rats.

Association of Polymorphisms in the Calpain I Gene with Meat Quality Traits in Yanbian Yellow Cattle of China

Xin, Jin,Zhang, Li-Chun,Li, Zhao-Zhi,Liu, Xiao-Hui,Jin, Hai-Guo,Yan, Chang-Guo Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.1

The calpain I (CAPN1) gene is an important marker for meat tenderness and marbling score in the bovine, but there were no studies to determine whether the CAPN1 gene had an association with other meat quality traits. In this study, we examined the relation between genetic polymorphisms of the CAPN1 gene and some meat quality traits in Yanbian Yellow Cattle of China. By PCRSSCP and gene sequencing in 321 unrelated Yanbian yellow cattle, twenty seven single nucleotide polymorphisms (SNPs) were detected in CAPN1, two existed SNPs in exon 8 and exon 17 resulted in the change of AA at F311S and M599V, respectively, and the otherpolymorphisms were at intron 7, 8, 14, 16 and 17. There were different preponderant genotypes at the corresponding gene locus and all genotypes were not associated with tenderness but other meat traits. This is the first study of the relationship between CAPN1 and meat quality besides tenderness in Yanbian yellow cattle of China.

Sick sinus syndrome associated with Erdheim- Chester disease was reversed by interferon-alpha treatment

( Xinli Guo ),( Yuanwei Xu ),( Ke Wan ),( Yucheng Chen ) 대한내과학회 2022 The Korean Journal of Internal Medicine Vol.37 No.1

miR-181b as a Potential Molecular Target for Anticancer Therapy of Gastric Neoplasms

Guo, Jian-Xin,Tao, Qing-Song,Lou, Peng-Rong,Chen, Xiao-Chun,Chen, Jun,Yuan, Guang-Bo Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objective: MicroRNAs (miRNAs) play important roles in carcinogenesis. The aim of the present study was to explore the effects of miR-181b on gastric cancer. Methods: The expression level of miR-181b was quantified by qRT-PCR. MTT, flow cytometry and matrigel invasion assays were used to test proliferation, apoptosis and invasion of miR-181b stable transfected gastric cancer cells. Results: miR-181b was aberrantly overexpressed in gastric cancer cells and primary gastric cancer tissues. Further experiments demonstrated inducible expression of miR-181b by Helicobacter pylori treatment. Cell proliferation, migration and invasion in the gastric cancer cells were significantly increased after miR-181b transfection and apoptotic cells were also increased. Furthermore, overexpression of miR-181b downregulated the protein level of tissue inhibitor of metalloproteinase 3 (TIMP3). Conclusion: The upregulation of miR-181b may play an important role in the progress of gastric cancer and miR-181b maybe a potential molecular target for anticancer therapeutics of gastric cancer.

China's Strategies for Resolving the North Korean Nuclear Crisis

Guo Xinning 국방대학교 국가안전보장문제연구소 2006 The Korean Journal of Security Affairs Vol.11 No.2

The international society has been very much concerned about the latest situation on the Korean Peninsula after the Democratic Peoples' Republic of Korea's (DPRK) nuclear test on 9 October 2006 and has shown ever-increasing anxiety over the prospecter a peaceful solution to the crisis. What will be the outcome of the crisis? Is there hope for the Peninsula to come out ofthe crisis peacefully? What are the best options for the solution of the issue? There are different views among internationa1 observers on the questions. ln the author's personal point of view, a comprehenslve and dipomatic solution must be pursued should the international society wish to avoid a catastrophic situation in the future.

China`s Strategies for Resolving the North Korean Nuclear Crisis

( Xin Ning Guo ) 국방대학교 안보문제연구소 2006 The Korean Journal of Security Affairs Vol.11 No.2

Two-Step Oxidation of Refractory Gold Concentrates with Different Microbial Communities^s

( Guo-hua Wang ),( Jian-ping Xie ),( Shou-peng Li ),( Yu-jie Guo ),( Ying Pan ),( Haiyan Wu ),( Xin-xing Liu ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.11

Bio-oxidation is an effective technology for treatment of refractory gold concentrates. However, the unsatisfactory oxidation rate and long residence time, which cause a lower cyanide leaching rate and gold recovery, are key factors that restrict the application of traditional bio-oxidation technology. In this study, the oxidation rate of refractory gold concentrates and the adaption of microorganisms were analyzed to evaluate a newly developed two-step pretreatment process, which includes a high temperature chemical oxidation step and a subsequent bio-oxidation step. The oxidation rate and recovery rate of gold were improved significantly after the two-step process. The results showed that the highest oxidation rate of sulfide sulfur could reach to 99.01 % with an extreme thermophile microbial community when the pulp density was 5%. Accordingly, the recovery rate of gold was elevated to 92.51%. Meanwhile, the results revealed that moderate thermophiles performed better than acidophilic mesophiles and extreme thermophiles, whose oxidation rates declined drastically when the pulp density was increased to 10% and 15%. The oxidation rates of sulfide sulfur with moderate thermophiles were 93.94% and 65.73% when the pulp density was increased to 10% and 15%, respectively. All these results indicated that the twostep pretreatment increased the oxidation rate of refractory gold concentrates and is a potential technology to pretreat the refractory sample. Meanwhile, owing to the sensitivity of the microbial community under different pulp density levels, the optimization of microbial community in bio-oxidation is necessary in industry.

Fix-frequency robust power model predictive control method for three-phase PWM rectifiers under unbalanced grid conditions

Guo, Xin,Xiao, Min,Gao, Yu-er,Wang, Qingyu,Wan, Yihao The Korean Institute of Power Electronics 2020 JOURNAL OF POWER ELECTRONICS Vol.20 No.5

Under unbalanced grid conditions, the controller design of a three-phase pulse width modulation (PWM) rectifier is based on an instantaneous power model. By calculating the current references of the converter according to the instantaneous power model, traditional voltage-oriented control (VOC) methods realize the positive-sequence and negative-sequence active and reactive current control of the converter separately using the proportional-integral (PI) controller. However, due to the inner current loop control structure of the traditional VOC method, it is impossible to realize the regulation of all six power components in a common instantaneous power model under an unbalanced grid. Meanwhile, the control performance of the traditional VOC method with a PI controller is degraded under the circuit parameters uncertainty condition. In this paper, a fixed switching frequency robust power model predictive control method (FRP-MPC) is proposed for three-phase PWM rectifiers under the unbalanced grid condition. The proposed control strategy has a number of advantages. An improved instantaneous power model is used for the fixed switching frequency model predictive controller design under the unbalanced grid condition, which has less power variables than the common instantaneous power model. The robustness of the MPC controller is improved by adding a robust item into the predictive model under circuit parameters uncertainty. Simulation and experiment results verify the effectiveness of the proposed control method.