MCT2 overexpression promotes recovery of cognitive function by increasing mitochondrial biogenesis in a rat model of stroke

Xiaorong Yu,Rui Zhang,Cunsheng Wei,Yuanyuan Gao,Yanhua Yu,Lin Wang,Junying Jiang,Xuemei Zhang,Junrong Li,Xuemei Chen 한국통합생물학회 2021 Animal cells and systems Vol.25 No.2

Monocarboxylate transporter 2 (MCT2) is the predominant monocarboxylate transporter expressed by neurons. MCT2 plays an important role in brain energy metabolism. Stroke survivors are at high risk of cognitive impairment. We reported previously that stroke-induced cognitive impairment was related to impaired energy metabolism. In the present study, we report that cognitive function was impaired after stroke in rats. We found that MCT2 expression, but not that of MCT1 or MCT4, was markedly decreased in the rat hippocampus at 7 and 28 days after transient middle cerebral artery occlusion (tMCAO). Moreover, MCT2 overexpression promoted recovery of cognitive function after stroke. The molecular mechanism underlying these effects may be related to an increase in adenosine monophosphate-activated protein kinase-mediated mitochondrial biogenesis induced by overexpression of MCT2. Our findings suggest that MCT2 activation ameliorates cognitive impairment after stroke.

Circ_0075960 targets the miR-202-5p/CTNND1 axis to promote the growth and migration of endometrial carcinoma cells via regulating Wnt/β-catenin signaling activity

Yan Nian,Xiaorong Li,Jingwen Ma,Ting Gao,Dan Liu 대한부인종양학회 2023 Journal of Gynecologic Oncology Vol.34 No.1

Background: Endometrial carcinoma (EC) is one of the most common malignant tumors of the female reproductive tract, involving multiple molecular alterations. Circular RNA (circRNA) dysregulation is frequently observed in EC tissues, suggesting the involvement of circRNA in EC development. We aimed to investigate the role of circ_0075960 in EC. Methods: Real-time quantitative polymerase chain reaction (RT-qPCR) and western blot assays were applied for expression analysis. CCK-8, EdU, colony formation, flow cytometry and wound healing assays were employed for functional analysis. The predicted binding relationship between miR-202-5p and circ_0075960 or CTNND1 was validated by dual-luciferase reporter experiment. In vivo animal models were constructed in nude mice to verify the role of circ_0075960 in tumor growth. Results: Circ_0075960 and CTNND1 were upregulated, while miR-202-5p was downregulated in EC. Knockdown of circ_0075960 induced EC cell apoptosis, suppressed cell proliferation and migration, and repressed tumor growth in animal models. MiR-202-5p was targeted by circ_0075960 and it directly bound to CTNND1 3’UTR. The inhibition of circ_0075960 knockdown or miR-202-5p enrichment on EC cell proliferation and migration was reversed by miR-202-5p depletion or CTNND1 overexpression, respectively. Circ_0075960 targeted miR-202-5p to positively regulate CTNND1 expression. Moreover, circ_0075960 knockdown weakened the activity of Wnt/β-catenin signaling via targeting the miR-202-5p/CTNND1 axis. Conclusion: Circ_0075960 targets the miR-202-5p/CTNND1 axis to modulate Wnt/β-catenin signaling activity, thus contributing to the malignant development of EC.

Ethanol Induces Autophagy Regulated by Mitochondrial ROS in Saccharomyces cerevisiae

( Hongjuan Jing ),( Huanhuan Liu ),( Lu Zhang ),( Jie Gao ),( Haoran Song ),( Xiaorong Tan ) 한국미생물 · 생명공학회 2018 Journal of microbiology and biotechnology Vol.28 No.12

Ethanol accumulation inhibited the growth of Saccharomyces cerevisiae during wine fermentation. Autophagy and the release of reactive oxygen species (ROS) were also induced under ethanol stress. However, the relation between autophagy and ethanol stress was still unclear. In this study, expression of the autophagy genes ATG1 and ATG8 and the production of ROS under ethanol treatment in yeast were measured. The results showed that ethanol stress very significantly induced expression of the ATG1 and ATG8 genes and the production of hydrogen peroxide (H₂O₂) and superoxide anion (O₂ ^·-). Moreover, the atg1 and atg8 mutants aggregated more H₂O₂ and O₂ ^·- than the wild-type yeast. In addition, inhibitors of the ROS scavenging enzyme induced expression of the ATG1 and ATG8 genes by increasing the levels of H₂O₂ and O₂ ^·-. In contrast, glutathione (GSH) and N-acetylcystine (NAC) decreased ATG1 and ATG8 expression by reducing H₂O₂ and O₂ ^·- production. Rapamycin and 3-methyladenine also caused an obvious change in autophagy levels and simultaneously altered the release of H₂O₂ and O₂ ^·-. Finally, inhibitors of the mitochondrial electron transport chain (mtETC) increased the production of H₂O₂ and O₂ ^·- and also promoted expression levels of the ATG1 and ATG8 genes. In conclusion, ethanol stress induced autophagy which was regulated by H₂O₂ and O₂ ^·- derived from mtETC, and in turn, the autophagy contributed to the elimination H₂O₂ and O₂ ^·-.

Transient Expression of the GUS Gene in a Unicellular Marine Green Alga, Chlorella sp. MACC/C95, via Electroporation

Wang, Changhai,Wang, Yiyun,Su, Qiao,Gao, Xiaorong Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.2

A transient expression system for a unicellular marine green alga, Chlorella sp. MACC/C95, was developed using a reporter GUS gene coded for by plasmid pBI121. The results demonstrated a high transformation efficiency could be achieved by using electroporation to deliver DNA into intact cells and the CaMV35S promoter to drive the foreign gene expression in Chlorella sp. MACC/C95. The use of a carrier DNA coupled with osmosis treatment improved the transformation efficiency, while linearization of the plasmid had minor effects. Investigation of the effects of DNA concentration and growth phases of Chlorella sp. MACC/C95 on transformation efficiency indicated that the highest level of transient expression was observed when $6{\mu}g\;mL^{-1}$ of plasmid DNA and cells $2{\sim}6$ days old were used.

Effect of Acrylic Content on the Properties of the Polyurethane/Polyacrylate Composite Emulsion

Guoyan Ma,Yiding Shen,Ruimin Gao,Xiaorong Wang 한국고분자학회 2017 폴리머 Vol.41 No.1

A polyurethane/polyacrylate (PUA) composite emulsion was synthesized by using polyurethane (PU) as seeds with soap-free emulsion polymerization, in which methyl methacrylate (MMA) and butyl acrylate (BA) were used as main acrylic monomers. The effect of acrylic contents and “stiff” and “soft” weight ratio of acrylic monomers on the properties of the films were investigated. The Fourier transform infrared (FTIR) results showed that acrylic monomers were involved in the emulsion copolymerization. The optimum composition of PUA composite formation was obtained when the polyacrylate (PA) content was 20%, in which the weight ratio of MMA and BA was 2/1. With the increment of PA content, the decomposition temperature increased.

Transient Expression of the GUS Gene in a Unicellular Marine Green Alga, Chlorella sp. MACC/C95, via Electroporation

Changhai Wang,Yiyun Wang,Qiao Su,Xiaorong Gao 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.2

A transient expression system for a unicellular marine green alga, Chlorella sp. MACC/C95, was developed using a reporter GUS gene coded for by plasmid pBI121. The results demonstrated a high transformation efficiency could be achieved by using electroporation to deliver DNA into intact cells and the CaMV35S promoter to drive the foreign gene expression in Chlorella sp. MACC/C95. The use of a carrier DNA coupled with osmosis treatment improved the transformation efficiency, while linearization of the plasmid had minor effects. Investigation of the effects of DNA concentration and growth phases of Chlorella sp. MACC/C95 on transformation efficiency indicated that the highest level of transient expression was observed when 6 μg mL-1 of plasmid DNA and cells 2~6 days old were used.