A Multi-center Survey of HPV Knowledge and Attitudes Toward HPV Vaccination among Women, Government Officials, and Medical Personnel in China

Zhao, Fang-Hui,Tiggelaar, Sarah M.,Hu, Shang-Ying,Zhao, Na,Hong, Ying,Niyazi, Mayinuer,Gao, Xiao-Hong,Ju, Li-Rong,Zhang, Li-Qin,Feng, Xiang-Xian,Duan, Xian-Zhi,Song, Xiu-Ling,Wang, Jing,Yang, Yun,Li, Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objectives: To assess knowledge of HPV and attitudes towards HPV vaccination among the general female population, government officials, and healthcare providers in China to assist the development of an effective national HPV vaccination program. Methods: A cross-sectional epidemiologic survey was conducted across 21 urban and rural sites in China using a short questionnaire. 763 government officials, 760 healthcare providers, and 11,681 women aged 15-59 years were included in the final analysis. Data were analyzed using standard descriptive statistics and logistic regression. Results: Knowledge of HPV among the general female population was low; only 24% had heard of HPV. Less than 20% of healthcare providers recognized sexually na$\ddot{i}$ve women as the most appropriate population for HPV vaccination. There was high acceptance of the HPV vaccine for all categories of respondents. Only 6% of women were willing to pay more than US $300 for the vaccine. Conclusions: Aggressive education is necessary to increase knowledge of HPV and its vaccine. Further proof of vaccine safety and efficacy and government subsidies combined with increased awareness could facilitate development and implementation of HPV vaccination in China.

Enhanced D-ribose production by genetic modification and medium optimization in Bacillus subtilis 168

Chen Zhao,Xiang-Ying Zhao,Jian-Jun Liu,Jun-Jiao Zhang,Jia-Xiang Zhang,Li-He Zhang 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.5

D-ribose, a five-carbon sugar with diverse applications, is mainly produced by transketolase (tkt)-deficient Bacillus subtilis (B. Subtilis). We constructed B.subtilis SFR-3A by replacing the corresponding sites of B. subtilis 168 with the mutation site of tkt in the “wild” D-ribose high-producing strain B. subtilis SFR-4, resulting in 5.29 g/L of Dribose. In the meantime, B.subtilis SFR-159 was constructed by completely removing the tkt gene of B. subtilis 168 with a higher production of 6.21 g/L. Through medium optimization, batch fermentation of SFR-3A and SFR-159 gave the best result of 27.56 g/L and 29.89 g/L, which corresponds to productivity of 0.51 g/L/h and 0.41 g/L/h, respectively. In this work, SFR-3A showed more latent capacity over SFR-159 as to productivity and had greater potential to serve as a platform for D-ribose production.

Compressive behavior of profiled double skin composite wall

Ying Qin,Yong-Wei Li,Yu-Sen Su,Xu-Zhao Lan,Yuan-De Wu,Xiang-Yu Wang 국제구조공학회 2019 Steel and Composite Structures, An International J Vol.30 No.5

Profiled composite slab has been widely used in civil engineering due to its structural merits. The extension of this concept to the bearing wall forms the profiled composite wall, which consists of two external profiled steel plates and infill concrete. This paper investigates the structural behavior of this type of wall under axial compression. A series of compression tests on profiled composite walls consisting of varied types of profiled steel plate and edge confinement have been carried out. The test results are evaluated in terms of failure modes, load-axial displacement curves, strength index, ductility ratio, and load-strain response. It is found that the type of profiled steel plate has influence on the axial capacity and strength index, while edge confinement affects the failure mode and ductility. The test data are compared with the predictions by modern codes such as AISC 360, BS EN 1994-1-1, and CECS 159. It shows that BS EN 1994-1-1 and CECS 159 significantly overestimate the actual compressive capacity of profiled composite walls, while AISC 360 offers reasonable predictions. A method is then proposed, which takes into account the local buckling of profiled steel plates and the reduction in the concrete resistance due to profiling. The predictions show good correlation with the test results.

Structural behavior of the stiffened double-skin profiled composite walls under compression

Ying Qin,Yong-Wei Li,Xu-Zhao Lan,Yu-Sen Su,Xiang-Yu Wang,Yuan-De Wu 국제구조공학회 2019 Steel and Composite Structures, An International J Vol.31 No.1

Steel-concrete composite walls have been proposed and developed for applications in various types of structures. The double-skin profiled composite walls, as a natural development of composite flooring, provide structural and architectural merits. However, adequate intermediate fasteners between profiled steel plates and concrete core are required to fully mobilize the composite action and to improve the structural behavior of the wall. In this research, two new types of fasteners (i.e., threaded rods and vertical plates) were proposed and three specimens with different fastener types or fastener arrangements were tested under axial compression. The experimental results were evaluated in terms of failure modes, axial load versus axial displacement response, strength index, ductility index, and load-strain relationship. It was found that specimen with symmetrically arranged thread rods sustained more stable axial strain than that with staggered arranged threaded rods. Meanwhile, vertical plates are more suitable for practical use since they provide stronger confinement to profiled steel plate and effectively prevent the steel plate from early local buckling, which eventually enhance the composite action and increase the axial compressive capacity of the wall. The calculation methods were then proposed and good agreement was observed between the test results and the predicted results.

CA1 Modulates the Osteogenic Differentiation of Dental Follicle Stem Cells by Activating the BMP Signaling Pathway In Vitro

Zhao Jin-ze,Ge Ying-Ying,Xue Ling-fa,Xu Yao-xiang,Yue Jin,Li Cong,Xiao Wen-lin 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.6

Background: Carbonic anhydrase 1 (CA1) has been found to be involved in osteogenesis and osteoclast in various human diseases, but the molecular mechanisms are not completely understood. In this study, we aim to use siRNA and lentivirus to reduce or increase the expression of CA1 in Dental follicle stem cells (DFSCs), in order to further elucidate the role and mechanism of CA1 in osteogenesis, and provide better osteogenic growth factors and stem cell selection for the application of bone tissue engineering in alveolar bone fracture transplantation. Methods: The study used RNA interference and lentiviral vectors to manipulate the expression of the CA1 gene in DFSCs during in vitro osteogenic induction. The expression of osteogenic marker genes was evaluated and changes in CA1, alkaline phosphatase (ALP), Runt-related transcription factor 2 (RUNX2), and Bone morphogenetic proteins (BMP2) were measured using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting (WB). The osteogenic effect was assessed through Alizarin Red staining. Results: The mRNA and protein expression levels of CA1, ALP, RUNX2, and BMP2 decreased distinctly in the si-CA1 group than other groups (p < 0.05). In the Lentivirus-CA1 (LV-CA1) group, the mRNA and protein expressions of CA1, ALP, RUNX2, and BMP2 were amplified to varying degrees than other groups (p < 0.05). Apart from CA1, BMP2 (43.01%) and ALP (36.69%) showed significant upregulation (p < 0.05). Alizarin red staining indicated that the LV-CA1 group produced more calcified nodules than other groups, with a higher optical density (p < 0.05), and the osteogenic effect was superior. Conclusions: CA1 can impact osteogenic differentiation via BMP related signaling pathways, positioning itself upstream in osteogenic signaling pathways, and closely linked to osteoblast calcification and ossification processes. Background: Carbonic anhydrase 1 (CA1) has been found to be involved in osteogenesis and osteoclast in various human diseases, but the molecular mechanisms are not completely understood. In this study, we aim to use siRNA and lentivirus to reduce or increase the expression of CA1 in Dental follicle stem cells (DFSCs), in order to further elucidate the role and mechanism of CA1 in osteogenesis, and provide better osteogenic growth factors and stem cell selection for the application of bone tissue engineering in alveolar bone fracture transplantation. Methods: The study used RNA interference and lentiviral vectors to manipulate the expression of the CA1 gene in DFSCs during in vitro osteogenic induction. The expression of osteogenic marker genes was evaluated and changes in CA1, alkaline phosphatase (ALP), Runt-related transcription factor 2 (RUNX2), and Bone morphogenetic proteins (BMP2) were measured using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting (WB). The osteogenic effect was assessed through Alizarin Red staining. Results: The mRNA and protein expression levels of CA1, ALP, RUNX2, and BMP2 decreased distinctly in the si-CA1 group than other groups (p < 0.05). In the Lentivirus-CA1 (LV-CA1) group, the mRNA and protein expressions of CA1, ALP, RUNX2, and BMP2 were amplified to varying degrees than other groups (p < 0.05). Apart from CA1, BMP2 (43.01%) and ALP (36.69%) showed significant upregulation (p < 0.05). Alizarin red staining indicated that the LV-CA1 group produced more calcified nodules than other groups, with a higher optical density (p < 0.05), and the osteogenic effect was superior. Conclusions: CA1 can impact osteogenic differentiation via BMP related signaling pathways, positioning itself upstream in osteogenic signaling pathways, and closely linked to osteoblast calcification and ossification processes.

Roadway Engineering Mechanical Properties and Roadway Structural Instability Mechanisms in Deep Wells

Xiang-Rui Meng,Rui Peng,Guang-Ming Zhao,Ying-Ming Li 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.5

We proposed a new classification method for stress-bearing structures in very-deep roadways. We conducted tests for roadwayengineeringmechanical properties, including rock mechanical tests and ground stress measurement of two caverns in very-deepwells. We suggested a classification method for stress-bearing structures based on shear stress. Tests revealed that rock strength in theshear direction was the lowest; the stress distributions of acoustic emission and hollow inclusion were highly similar. Based on theroadway-engineering mechanical properties of two caverns and numerical simulation and in-situ tests, the key bearing structureswere classified based on the concentrated shear stress. In deep caverns, shear stress was more concentrated on the softer surroundingrock, the key bearing structure areas, and the more seriously fractured surrounding rocks. Using the loose circle in-situ test, wecompared the classification method of the key bearing structures with other classification methods. The results revealed agreementwith the classification methods used for the key bearing structures. The deformation in-situ test showed that the strata convergence ofconcentrated shear stress areas developed quickly. Therefore, the phenomena of concentrated shear stress and the expanded range ofkey bearing structures are the structural instability mechanisms of deep roadways.

A Filament Level Analysis on 3-D Orthogonal Weave Microgeometry Modeling under Different Yarn Tension

Ying Ma,Yueyan Liu,Congying Deng,Xiang Chen,Yang Zhao,Sheng Lu,Youqi Wang 한국섬유공학회 2020 Fibers and polymers Vol.21 No.10

3-D woven textile is generated through the weaving process. Yarn is a non-continuum material domain, thestructure of which is determined by inter fiber movement. In this study, the micro-geometry of 3-D orthogonal weave isgenerated at filament level predicting through the weaving process implementing the digital element approach (DEA). First,the basic concepts and explicit algorithm of DEA is introduced. The method of calculating adjusted digital fiber materialproperty in terms of discretization resolution is proposed. Second, the unit-cell topology of 3-D orthogonal weave is definedby a position matrix. The calculation of potential energy of the cell is derived. At last, a dynamic weaving process is designedto investigate the effect of yarn tension on fabric micro-geometry and cell energy. 4 unit-cells are generated under 4 sets oftension combination. Results show that as the fabric thickness decrease, the filaments of the weft yarns move towards thecenter. The weft yarns at the top and bottom deform into their final shape first. The applied tension on weaver plays adominant role in determining fabric thickness and convergence speed. By comparing the numerical results with themicroscope pictures taken from the actual specimen, it is concluded that the fabric micro-geometry produced by tensioncombination 4 closely matches the experimental results.

Notes : Construction of Conjugative Gene Transfer System Between E. coli and Moderately Thermophilic, Extremely Acidophilic Acidithiobaciilus caldus MTH-04

( Xiang Mei Liu ),( Jian Qun Lin ),( Zheng Zhang ),( Jiang Bian ),( Qing Zhao ),( Ying Liu ),( Jian Qiang Lin ),( Wang Ming Yan ) 한국미생물 · 생명공학회 2007 Journal of microbiology and biotechnology Vol.17 No.1

Screening of MicroRNA in Patients with Esophageal Cancer at Same Tumor Node Metastasis Stage with Different Prognoses

Zhao, Bao-Sheng,Liu, Shang-Guo,Wang, Tian-Yun,Ji, Ying-Hua,Qi, Bo,Tao, Yi-Peng,Li, Han-Chen,Wu, Xiang-Nan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Patients at the same pathological stage of esophageal cancer (EC) that received the same surgical therapy by the same surgeon may have distinct prognoses. The current study aimed to explore the possibility of differentially-expressed microRNAs (miRNAs) underlying this phenomenon. Samples were collected from EC patients at the same tumor node metastasis (TNM) stage but with different prognoses. Paracancerous normal tissues were taken as controls. The specimens were histopathologically analyzed. Differentially-expressed miRNAs were analyzed using real-time quantitative reverse transcription polymerase chain reaction. Compared with patients with poor prognosis, those with good prognosis exhibited 88 two-fold or more than two-fold increased miRNA fragments and 4 half-decreased miRNAs. The most noticeably up-regulated miRNAs included hsa-miR-31, hsa-miR-196b, hsa-miR-652, hsa-miR-125a-5p, hsa-miR-146b, hsa-miR-200c, hsa-miR-23b, hsa-miR-29a, hsa-miR-186, hsa-miR-205, hsa-miR-376a, hsa-miR-410, hsa-miR-532-3p, and hsa-miR-598, whereas the most significantly-downregulated miRNAs were hsa-let-7e, hsa-miR-130b, and hsa-miR-103. EC patients at same TNM stage but with different prognoses show differentially-expressed miRNAs.

Transplantation of Wnt5a-modified NSCs promotes tissue repair and locomotor functional recovery after spinal cord injury

Li Xiang,Peng Zhiming,Long Lingli,Lu Xiaofang,Zhu Kai,Tuo Ying,Chen Ningning,Zhao Xiaoyang,Wang Le,Wan Yong 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Traditional therapeutic strategies for spinal cord injury (SCI) are insufficient to repair locomotor function because of the failure of axonal reconnection and neuronal regeneration in the injured central nervous system (CNS). Neural stem cell (NSC) transplantation has been considered a potential strategy and is generally feasible for repairing the neural circuit after SCI; however, the most formidable problem is that the neuronal differentiation rate of NSCs is quite limited. Therefore, it is essential to induce the neuronal differentiation of NSCs and improve the differentiation rate of NSCs in spinal cord repair. Our results demonstrate that both Wnt5a and miRNA200b-3p could promote NSC differentiation into neurons and that Wnt5a upregulated miRNA200b-3p expression through MAPK/JNK signaling to promote NSC differentiation into neurons. Wnt5a could reduce RhoA expression by upregulating miRNA200b-3p expression to inhibit activation of the RhoA/Rock signaling pathway, which has been reported to suppress neuronal differentiation. Overexpression of RhoA abolished the neurogenic capacity of Wnt5a and miRNA200b-3p. In vivo, miRNA200b-3p was critical for Wnt5a-induced NSC differentiation into neurons to promote motor functional and histological recovery after SCI by suppressing RhoA/Rock signaling. These findings provide more insight into SCI and help with the identification of novel treatment strategies.