Cloning and characterization of the cardiac-specific Lrrc10 promoter

( Xiong Wei Fan ),( Qing Yang ),( You Liang Wang ),( Yan Zhang ),( Jian Wang ),( Jia Jia Yuan ),( Yong Qing Li ),( Yue Qun Wang ),( Yun Deng ),( Wu Zhou Yuan ),( Xiao Yang Mo ),( Yong Qi Wan ),( Karen 생화학분자생물학회 2011 BMB Reports Vol.44 No.2

Leucine-rich repeat containing protein 10 (LRRC10) is characterized as a cardiac-specific gene, suggesting a role in heart development and disease. A severe cardiac morphogenic defect in zebrafish morphants was recently reported but a contradictory result was found in mice, suggesting a more complicated molecular mechanism exists during mouse embryonic development. To elucidate how LRRC10 is regulated, we analyzed the 5`enhancer region approximately 3 kilo bases (kb) upstream of the Lrrc10 start site using luciferase reporter gene assays. Our characterization of the Lrrc10 promoter indicates it possesses complicated cis-and trans-acting elements. We show that GATA4 and MEF2C could both increase transcriptional activity of Lrrc10 promoter individually but that they do not act synergistically, suggesting that there exists a more complex regulation pattern. Surprisingly, knockout of Gata4 and Mef2c binding sites in the 5`enhancer region (-2,894/-2,889) didn`t change the transcriptional activity of the Lrrc10 promoter and the likely GATA4 binding site identified was located in a region only 100 base pair (bp) upstream of the promoter. Our data provides insight into the molecular regulation of Lrrc10 expression, which probably also contributes to its tissue-specific expression. [BMB reports 2011; 44(2): 123-128]

Radiosensitization Effect of Overexpression of Adenovirus-mediated SIRT6 on A549 Non-small Cell Lung Cancer Cells

Cai, Yong,Sheng, Zhao-Ying,Liang, Shi-Xiong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Objective: To explore the radiosensitization effect of overexpression of silent information regulator 6 (SIRT6) on A549 non-small cell lung cancer (NSCLC) cells. Methods: Adenovirus vector Ad-SIRT6 causing overexpression of SIRT6 was established. Western blotting and MTT assay were adopted to detect the level of SIRT6 protein and the inhibitory rate of A549 cell proliferation after different concentrations of adenovirus transduction (0, 25, 100, 200, and 400 pfu/cell) for 24 h. Control group, Ad-null group and Ad-SIRT6 group were designed in this experiment and virus concentration of the latter two groups was 200 pfu/cell. Colony formation assays were employed to test survival fraction (SF) of the 3 groups after 0, 2, 4, 6, 8, 10 X-ray irradiation. Flow cytometry was used to detect the status of cell cycle of 3 groups after 48 h of 4Gy X-ray irradiation and Western blotting was used to determine the expression of apoptosis-related genes of 3 groups after 48 h of 4GyX-ray irradiation. Results: In the range of 25~400 pfu/cell, the inhibitory rate of A549 cell proliferation increased as adenovirus concentration raised. The inhibitory rates under the concentrations of 0, 25, 100, 200, and 400 pfu/cell were 0%, $4.23{\pm}0.34%$, $12.7{\pm}2.57%$, $22.6{\pm}3.38%$, $32.2{\pm}3.22%$, $38.7{\pm}4.09%$ and $47.8{\pm}5.58%$ and there were significantly differences among groups (P<0.05). SF in Ad-SIRT6 group was lower than Ad-null and control groups after 4~10Gy X-ray irradiation (P<0.05) and the sensitization enhancement ratio (SER) was 1.35 when compared with control group. Moreover, after 48 h of 4Gy X-ray irradiation, there appeared a significant increase in G1-phase cell proportion, upregulated expression of the level of apoptosis-promoting genes (Bax and Cleaved caspase-3), but a obvious decline in S-phase and G2-phase cell proportion and a significant decrease of the level of apoptosis-inhibiting gene (Bal-2) in the Ad-SIRT6 group (P<0.05). Conclusion: The over-expression of adenovirus-mediated SIRT6, which has radiosensitization effect on A549 cells of NSCLC, can inhibit the proliferation of A549 cells and cause G0/G1 phase retardation as well as induce apoptosis of cells.

Finite-time Fault-tolerant Control of Robotic Systems with Uncertain Dynamics

Changgui Xiong,Liang Yang,Bing Zhou,Yong Chen 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.8

Control of nonlinear robotic systems suffering from actuator failure is a significant, yet challenging problem. In this paper, an observer-based fault-tolerant control method is proposed for robotic systems with external disturbances and modeling discrepancy. Technically, based on the state transform, an unknown input observer is designed to estimate the lumped unknown input. With the aid of the proposed observer, the estimate error is ensured to decay to zero within finite time. Subsequently, the estimated unknown input is utilized to compensate the compound unknown input of the faulty system. The analytical results proved that the tracking error of the closedloop system converges to zero in finite-time, even in the presence of actuator faults. Finally, simulation results are provided to demonstrate the effectiveness of the proposed scheme.

MiR-421 Regulates Apoptosis of BGC-823 Gastric Cancer Cells by Targeting Caspase-3

Wu, Jian-Hong,Yao, Yong-Liang,Gu, Tao,Wang, Ze-You,Pu, Xiong-Yong,Sun, Wang-Wei,Zhang, Xian,Jiang, Yi-Biao,Wang, Jian-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

MicroRNAs might act as oncogenes or tumor suppressors in cancer. Recent studies have shown that miR-421 is up-regulated in human gastric cancer. Here, we found that miR-421 was over-expressed in gastric cancer tissues and cell lines. Bioinformatics analysis predicted that the caspase-3 gene was a target of miR-421. Caspase-3 was negatively regulated by miR-421 at the post-transcriptional level. Bax and Bcl-2 were also regulated by miR-421. Moreover, tumor necrosis factor receptor-I and -II, death receptors in the apoptosis pathway, were up-regulated by miR-421. The over-expression of miR-421 promoted gastric cancer cell growth and inhibited apoptosis of the BGC-823 gastric cancer cell line. These observations indicate that miR-421 acts as a tumor promoter by targeting the caspase-3 gene and preventing apoptosis of gastric cancer cells through inhibition of caspase-3 expression. These findings contribute to our understanding of the functions of miR-421 in gastric cancer.

Study on Springback Properties of Different Orthodontic Archwires in Archwire Bending Process

Jiang Jin-gang,Wang Zhao,Zhang Yong-de,Jiang Ji-xiong,Niu Suo-liang,Liu Yi 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.12

The archwire bending is one of processes the most frequently used in the orthodontic treatment. Furthermore, the springback of sheet metal, which is defined as elastic recovery of the part during unloading, should be taken into consideration so as to produce formed archwire within acceptable tolerance limits. In this paper, the springback angle of different alloy archwires (including NiTi alloy wire, Beta-Ti alloy wires, Chinese stainless steel wires, and Australian stainless steel wires) were performed by the theoretical calculation based on large deformation theory and the finite element analysis. A series of numerical simulations has been conducted for the bending test, which physically simulates the actual bending of alloy archwire with a robotic apparatus. The finite element analysis of springback is shown to be very consistent with the theoretical calculation results.

Effect of Mechanical Alloying and Sintering Behavior on the Microstructure and Properties of NbMoTaWRe Refractory High Entropy Alloy

Tao Gu,Li‑Min Wang,Qiang Hu,Xiu‑Bing Liang,Dong‑Xing Fu,Yong‑Xiong Chen,Xin‑Ming Zhao,Yan‑Wei Sheng 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.11

An equiatomic refractory high-entropy alloy (RHEA) NbMoTaWRe is prepared by mechanical alloying (MA) and sparkplasma sintering (SPS). The effects of mechanical alloying and sintering behaviors on the microstructure and propertiesof the RHEA are investigated. After ball-milling for 30 h, the metastable and supersaturated MA powders with the bodycenteredcubic (BCC) structure are obtained. Then, the MA powders are sintered using the SPS method under the sinteringtemperature range of 1700–1900 °C, and the C atoms and WC introduced by the MA process reacts with the metastable andsupersaturated Ta/Nb phase of the MA powers to form the face-centered cubic (FCC) structure (Nb, Ta)C particles alongthe BCC matrix boundaries during the SPS process. The NbMoTaWRe alloy sintered at 1800 °C consisted of BCC matrixand FCC-type (Nb, Ta)C particles has high compactness (porosity fraction is 0.32%), fracture strength (2630 MPa), plasticstrain (6.82%), and hardness (992 ± 20 HV). These excellent properties of this RHEA are mainly attributed to the combinationof multi-effects, including sintering densification, grain refinement strengthening from the refined sizes (3.80 μm) BCCmatrix, precipitation strengthening from the (Nb, Ta)C particles, solid solution strengthening from multi-principal elementsand interstitial solid solution strengthening from C atoms dissolving into BCC matrix.

Macrophage-secreted Exosomes Delivering miRNA-21 Inhibitor can Regulate BGC-823 Cell Proliferation

Wang, Jian-Jun,Wang, Ze-You,Chen, Rui,Xiong, Jing,Yao, Yong-Liang,Wu, Jian-Hong,Li, Guang-Xin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.10

Exosomes, membranous nanovesicles, naturally carry bio-macromolecules or miRNA and play impoetant roles in tumor pathogenesis. Here, we showed that macrophages cell-derived exosomes can function as vehicles to deliver exogenous miR-21 inhibitor into BGC-823 gastric cancer cells. Exosomes loaded with miR-21inhibitor significantly increased miR-21 levels in BGC-823, but miR-21inhibitor loaded in exosomes exerted an opposite effect. miRNA transfected with exosomes had less cellular toxicity to host cells compared to conventional transfection methods. The miR-21inhibitor loaded exosomes promoted the migration ability and reduced apoptosis of BGC-823 gastric cancer cells. These observations indicate that miR-21 acts as a tumor promoter by targeting the PDCD4 gene and preventing apoptosis of gastric cancer cells through inhibition of PDCD4 expression. Furthermore, exosome -mediated miR-21 inhibitor delivery resulted in functionally more efficient inhibition and less cellular toxicity compared to conventional transfection methods. Similar approaches could be useful in modification of target biomolecules in vitro and in vivo. These findings contribute to our understanding of the functions of miR-21 and exosomes as a carrier for therapy of gastric cancer.

Study on the Real-Time Precise Orbit Biases Correction Technique for the GPS/VRS Network

LI Cheng-gang,HUANG Ding-fa,ZHOU Dong-wei,ZHOU Le-tao,XIONG Yong-liang,XU Rui 한국항해항만학회 2006 한국항해항만학회 학술대회논문집 Vol.2 No.-

A precise real-time method of using the IGS ultra rapid products (IGU) and the GPS broadcast ephemeris to calculate the VRS orbit corrections was presented here which was suited for GPS/VRS reference station network based positioning. Test data acquired from both the SGRSN (Sichuan GPS Reference Station Network) and SCIGN (Southern California integrated GPS network) were used to evaluate the performance of the modeling techniques. The new method was proven to be more precise and reliable compared with the existing conventional network-based orbit error interpolation method. It was shown that 0.004ppm relative accuracy was reached, namely the influence from the orbit bias for the RTK positioning within 100km area can be of sub-millimeter level.