A two-photon fluorescent probe for specific detection of hydrogen sulfide based on a familiar ESIPT fluorophore bearing AIE characteristics

Chen, L.,Wu, D.,Lim, C.,Kim, D.,Nam, S. J.,Lee, W.,Kim, G.,Kim, H.,Yoon, J. unknown 2017 Chemical communications Vol. No.

<P>Hydrogen sulfide (H2S) is a signaling gasotransmitter, which plays various roles in modulating the functions of different systems. In this study, a 'turn-on'' fluorescent probe for H2S was developed. The probe, whose design is based on a familiar excited state intramolecular proton transfer (ESIPT) fluorophore bearing aggregation-induced emission (AIE) characteristics, was found to exhibit an 80-fold fluorescence enhancement along with a large Stokes shift upon the addition of H2S. Furthermore, the probe was successfully utilized to detect H2S in HeLa cells with both one-photon and two-photon microscopy.</P>

A new constructive neural network method for noise processing and its application on stock market prediction

Xi, L.,Muzhou, H.,Lee, M.H.,Li, J.,Wei, D.,Hai, H.,Wu, Y. Elsevier Science, B.V 2014 Applied soft computing Vol.15 No.-

In this paper, in order to optimize neural network architecture and generalization, after analyzing the reasons of overfitting and poor generalization of the neural networks, we presented a class of constructive decay RBF neural networks to repair the singular value of a continuous function with finite number of jumping discontinuity points. We proved that a function with m jumping discontinuity points can be approximated by a simplest neural network and a decay RBF neural network in L<SUP>2</SUP>(@?) by each @? error, and a function with m jumping discontinuity point y=f(x),x@?E@?@?<SUP>d</SUP> can be constructively approximated by a decay RBF neural network in L<SUP>2</SUP>(@?<SUP>d</SUP>) by each ε>0 error. Then the whole networks will have less hidden neurons and well generalization in the same of the first part. A real world problem about stock closing price with jumping discontinuity have been presented and verified the correctness of the theory.

Saccharomyces cerevisiae Live Cells Decreased In vitro Methane Production in Intestinal Content of Pigs

Gong, Y.L.,Liao, X.D.,Liang, J.B.,Jahromi, M.F.,Wang, H.,Cao, Z.,Wu, Y.B. Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.6

An in vitro gas production technique was used in this study to elucidate the effect of two strains of active live yeast on methane ($CH_4$) production in the large intestinal content of pigs to provide an insight to whether active live yeast could suppress $CH_4$ production in the hindgut of pigs. Treatments used in this study include blank (no substrate and no live yeast cells), control (no live yeast cells) and yeast (YST) supplementation groups (supplemented with live yeast cells, YST1 or YST2). The yeast cultures contained $1.8{\times}10^{10}$ cells per g, which were added at the rates of 0.2 mg and 0.4 mg per ml of the fermented inoculum. Large intestinal contents were collected from 2 Duroc${\times}$Landrace${\times}$Yorkshire pigs, mixed with a phosphate buffer (1:2), and incubated anaerobically at $39^{\circ}C$ for 24 h using 500 mg substrate (dry matter (DM) basis). Total gas and $CH_4$ production decreased (p<0.05) with supplementation of yeast. The methane production reduction potential (MRP) was calculated by assuming net methane concentration for the control as 100%. The MRP of yeast 2 was more than 25%. Compared with the control group, in vitro DM digestibility (IVDMD) and total volatile fatty acids (VFA) concentration increased (p<0.05) in 0.4 mg/ml YST1 and 0.2 mg/ml YST2 supplementation groups. Proportion of propionate, butyrate and valerate increased (p<0.05), but that of acetate decreased (p<0.05), which led to a decreased (p<0.05) acetate: propionate (A: P) ratio in the both YST2 treatments and the 0.4 mg/ml YST 1 supplementation groups. Hydrogen recovery decreased (p<0.05) with yeast supplementation. Quantity of methanogenic archaea per milliliter of inoculum decreased (p<0.05) with yeast supplementation after 24 h of incubation. Our results suggest that live yeast cells suppressed in vitro $CH_4$ production when inoculated into the large intestinal contents of pigs and shifted the fermentation pattern to favor propionate production together with an increased population of acetogenic bacteria, both of which serve as a competitive pathway for the available H2 resulting in the reduction of methanogenic archaea.

Genetic Variation of H-FABP Gene and Association with Intramuscular Fat Content in Laiwu Black and Four Western Pig Breeds

Zeng, Y.Q.,Wang, G.L.,Wang, C.F.,Wei, S.D.,Wu, Y.,Wang, L.Y.,Wang, H.,Yang, H.L. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.1

This study was performed to detect genetic variation of the heart fatty acid-binding protein (H-FABP) gene by PCRRFLPs approach and its association with intramuscular fat (IMF) content. Data from 223 individuals, including one Chinese native pig breed and four western pig breeds, were analyzed. The results showed that for the H-FABP gene, there was one polymorphic HinfI site in the 5'-upstream region, whereas there were one HaeIII and one HinfI (marked as $HinfI^*$) polymorphic site in the second intron, respectively. The three PCR-RFLPs were present in all breeds tested. The allele frequencies, however, revealed significant differences between them (p<0.05). Furthermore, the allele frequency distribution of HinfI in the Laiwu Black and that of $HinfI^*$ in the Hampshire breed were at disequilibrium, which might be the result of selective breeding. Results also indicated that for HinfI, HaeIII and $HinfI^*$ HFABP RFLP, significant (p<0.05) contrasts of 0.78%, -0.69% and 0.72% were detected in the least square means of IMF content between the homozygous genotype HH and hh, DD and dd, BB and bb classes, respectively. It implied that the HHddBB genotype had the highest IMF content in this experimental population and these H-FABP RFLPs could serve, to some extent, as genetic markers for use in improvement of IMF content.

A Randomized, Controlled Trial of Sofosbuvir/Velpatasvir/Voxilaprevir or Sofosbuvir/Velpatasvir for 12 Weeks in DAA-Experienced Patients with GT1-6 HCV Infection: The POLARIS-4 Study

( Paul Kwo ),( Stefan Zeuzem ),( Steven L. Flamm ),( Myron Tong ),( John M Vierling ),( Stephen Pianko ),( Peter Buggisch ),( Victor de Lédinghen ),( Robert H. Hyland ),( Xiaoru Wu ),( Evguenia S. Sva 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: DAAs provide safe and highly efficacious therapies for HCV infection. However, the small proportion of patients who do not achieve a sustained virologic response with DAA-based regimens represent a population with an unmet medical need. Sofosbuvir(SOF) and velpatasvir(VEL) are pangenotypic inhibitors of the HCV NS5B and NS5A proteins, respectively, and voxilaprevir(VOX) is a pangenotypic HCV NS3/4A protease inhibitor. This study evaluates treatment with a SOF/VEL/VOX for 12weeks and a SOF/VEL for 12weeks as salvage regimens in DAA-experienced patients who had not previously received an NS5A inhibitor. Methods: Patients with genotypes 1-3 were randomized 1:1 to receive open-label SOF/VEL/VOX or SOF/VEL for 12weeks, stratified according to genotype and cirrhosis status. Patients of all other genotypes were assigned to receive SOF/VEL/VOX for 12weeks. DAA-experienced patients who previously were treated with an NS5A inhibitor or with only an NS3/4A protease inhibitor in combination with ribavirin and Peg-IFN were excluded. The primary endpoint evaluates the superiority of the SVR12 of each treatment to a prespecified goal of 85%. Results: Of the 333 patients who were randomized and treated, 77% were male, 19% had the IL28B CC genotype, 46% had compensated cirrhosis and 43% had genotype 1 infection. Most patients had prior DAA experience with either an NS5B inhibitor alone(73%) or an N5SB inhibitor and an NS3/4A protease inhibitor(25%); the most common prior treatment regimens were SOF with ribavirin ±Peg-IFN and SOF combined with simeprevir. Treatment was well tolerated.No SAE was assessed to be attributable to study drug. Overall, SVR12 was achieved in 97%(177/182) of patients treated with SOF/VEL/VOX and 90%(136/151) patients treated with SOF/VEL. SOF/VEL/VOX met the prespecified 85% SVR12 performance goal(p<0.001); SOF/VEL did not. Conclusions: SOF/VEL/VOX for 12 weeks provides a safe, well tolerated and effective retreatment options for patients who did not previously achieve SVR following treatment with non-NS5A inhibitor-containing DAA regimens.

Performance of the CREAM-III Calorimeter

Moo Hyun Lee,Ho Seok Ahn,Ganel, O.,Ji Hye Han,Jeon, J.A.,Chan Ho Kim,Ki Chun Kim,Lutz, L.,Malinin, A.,GoWoon Na,Shinwoo Nam,Park, I.H.,Na Hee Park,Eun-Suk Seo,Vartanyan, A.,Walpole, P.,Jayoung Wu,Jong IEEE 2009 IEEE transactions on nuclear science Vol.56 No.3

<P>Cosmic Ray Energetics And Mass (CREAM) is a balloon-borne experiment to directly measure the elemental spectra of protons to iron nuclei with energies up to ~ 10<SUP>15</SUP> eV. Energies of these cosmic-ray particles are measured by an ionization calorimeter comprised of 20 layers of 1 radiation length thick tungsten plates and 20 layers of 0.5 mm diameter scintillating fibers. Each tungsten plate is 500 times 500 times 3.5 mm<SUP>3</SUP> and the fibers are grouped into fifty 1 cm wide ribbons. After construction, the CREAM-III calorimeter was tested at CERN, the European high energy physics lab, in the H2 beam line of the SPS. Following the CERN test, the calorimeter was integrated into the CREAM-III instrument, and flown successfully in the 3rd flight of the project, during the 2007/8 Antarctic campaign. We present the performance of the CREAM-III calorimeter in lab and beam tests.</P>

Intramuscular Administration of Zinc Metallothionein to Preslaughter Stressed Pigs Improves Anti-oxidative Status and Pork Quality

Li, L.L.,Hou, Z.P.,Yin, Y.L.,Liu, Y.H.,Hou, D.X.,Zhang, B.,Wu, G.Y.,Kim, S.W.,Fan, M.Z.,Yang, C.B.,Kong, X.F.,Tang, Z.R.,Peng, H.Z.,Deng, D.,Deng, Z.Y.,Xie, M.Y.,Xiong, H.,Kang, P.,Wang, S.X. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.5

This study was conducted to determine the effects of exogenous zinc-metallothionein (Zn-MT) on anti-oxidative function and pork quality. After feeding a corn-soybean meal-based diet for two weeks, 48 pigs ($Duroc{\times}Landrace{\times}Chinese\;Black Pig$) were assigned randomly to four groups. Pigs in Group 1 were maintained under non-stress conditions, whereas pigs in Groups 2, 3 and 4 were aggressively handled for 25 min to produce stress. Pigs in Groups 1, 2, 3, and 4 received intramuscular administration of saline (control group; CON), 0 (negative control group; NCON), 0.8 (low dose group; LOW), and 1.6 (high dose group; HIGH) mg rabbit liver Zn-MT per kg body weight, respectively. Pigs were slaughtered at 3 and 6 h post-injection. Zn-MT treatment increased (p<0.05) the activities of superoxide dismutase (SOD) and glutathione-peroxidase (GSH-PX) while decreasing the concentration of malondialdehyde (MDA) in liver. These responses were greater (p<0.05) at 6 h than at 3 h post Zn-MT injection. Zn-MT treatment increased (p<0.05) hepatic SOD mRNA levels in a time and dose-dependent manner and decreased (p<0.05) serum glutamate-pyruvate transaminase and lactate dehydrogenase activities (indicators of tissue integrity). Zn-MT administration decreased (p<0.05) lactate concentration and increased (p<0.05) pH and water-holding capacity in the longissimus thorasis meat. Collectively, our results indicate that intramuscular administration of Zn-MT to pre-slaughter stressed pigs improved tissue anti-oxidative ability and meat quality.

COMPLEX STOCHASTIC WHEELBASE PREVIEW CONTROL AND SIMULATION OF A SEMI-ACTIVE MOTORCYCLE SUSPENSION BASED ON HIERARCHICAL MODELING METHOD

Wu, L.,Chen, H.L. The Korean Society of Automotive Engineers 2006 International journal of automotive technology Vol.7 No.6

This paper presents a complex stochastic wheelbase preview control method of a motorcycle suspension based on hierarchical modeling method. As usual, a vehicle suspension system is controlled as a whole body. In this method, a motorcycle suspension with five Degrees of Freedom(DOF) is dealt with two local independent 2-DOF suspensions according to the hierarchical modeling method. The central dynamic equations that harmonize local relations are deduced. The vertical and pitch accelerations of the suspension center are treated as center control objects, and two local semi-active control forces can be obtained. In example, a real time Linear Quadratic Gaussian(LQG) algorithm is adopted for the front suspension and the combination of the wheelbase preview and LQG control method is designed for the rear suspension. The results of simulation show that the control strategy has less calculating time and is convenient to adopt different control strategies for front and rear suspensions. The method proposed in this paper provides a new way for the vibration control of multi-wheel vehicles.

Free vibrations of arbitrary quadrilateral thick plates with internal columns and uniform elastic edge supports by pb-2 Ritz method

Wu, L.H. Techno-Press 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.44 No.3

Free vibration analysis of arbitrary quadrilateral thick plates with internal columns and elastic edge supports is presented by using the powerful pb-2 Ritz method and Reddy's third order shear deformation plate theory. The computing domain of arbitrary quadrilateral planform is mapped onto a standard square form by coordinate transformation. The versatile pb-2 Ritz functions defined by the product of a two-dimensional polynomial and a basic function are taken to be the admissible functions. Substituting these displacement functions into the energy functional and minimizing the total energy by differentiation, leads to a typical eigenvalue problem, which is solved by a standard eigenvalue solver. Stiffness and mass matrices are numerically integrated over the plate by using Gaussian quadrature. The accuracy and efficiency of the proposed method are demonstrated through several numerical examples by comparison and convergency studies. A lot of numerical results for reasonable natural frequency parameters of quadrilateral plates with different combinations of elastic boundary conditions and column supports at any locations are presented, which can be used as a benchmark for future studies in this area.

Quantitative Trait Loci Mapping for Porcine Backfat Thickness

Wu, X.L.,Lee, C.,Jiang, J.,Peng, Y.L.,Yan, H.F.,Yang, S.L.,Xiao, B.N.,Liu, X.C.,Shi, Q.S. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.7

A partial genome scan using porcine microsatellites was carried out to detect quantitative trait loci (QTL) for backfat thickness (BFT) in a pig reference population. This population carried QTL on chromosomes 1, 13 and 18. The QTL on chromosome 1 was located between marker loci S0113 and SW1301. The QTL corresponded to very low density lipoprotein receptor gene (VLDLR) in location and in biological effects, suggesting that VLDLR might be a candidate gene. The QTL found on chromosome 13 was found between marker loci SWR1941 and SW864, but significance for the marker-trait association was inconsistent by using data with different generations. The QTL on chromosome 18 was discovered between markers S0062 and S0117, and it was in proximity of the regions where IGFBP3 and GHRHR were located. The porcine obese gene might be also a candidate gene for the QTL on chromosome 18. In order to understand genetic architecture of BFT better, fine mapping and positional comparative candidate gene analyses are necessary.