Study on Patent Forewarning Evaluation Index System of High & New Technology Enterprises

Wu Jian-long,Wang Hong-qi,Tao Wei-wei 인하대학교 정석물류통상연구원 2009 인하대학교 정석물류통상연구원 학술대회 Vol.2009 No.10

In the era of knowledge economy, patent technology is the key factor for high and new technology enterprises to participate in the global competition and maintain continually competitive advantage, meanwhile, monopoly rent and innovation rent of patent technology is also suffered from the challenge of profession technology change and patent strategy of competitors. Thus, regarding patent risk factors of hight and new technology enterprises, the paper constructs patent forewarning evaluation index which involves four aspects of patent protection, quantity, quality and industrialization level, and proposes using tactics of the evaluation index during the enterprises patent forewarning, so as to provide method support for high and new technology enterprises to operate their patent forewarning mechanism effectively.

Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes

Wu Long-Fei,Zhang Qin,Mo Xing-Bo,Lin Jun,Wu Yang-Lin,Lu Xin,He Pei,Wu Jian,Guo Yu-Fan,Wang Ming-Jun,Ren Wen-Yan,Deng Hong-Wen,Lei Shu-Feng,Deng Fei-Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Rheumatoid arthritis (RA) is an autoimmune disease characterized by infiltration of immune cells in the synovium. However, the crosstalk of immune cells and synovial fibroblasts is still largely unknown. Here, global miRNA screening in plasma exosomes was carried out with a custom microarray (RA patients vs. healthy controls = 9:9). A total of 14 exosomal miRNAs were abnormally expressed in the RA patients. Then, downregulated expression of exosomal miR-204-5p was confirmed in both the replication (RA patients vs. healthy controls = 30:30) and validation groups (RA patients vs. healthy controls = 56:60). Similar to the findings obtained in humans, a decreased abundance of exosomal miR-204-5p was observed in mice with collagen-induced arthritis (CIA). Furthermore, Spearman correlation analysis indicated that plasma exosomal miR-204-5p expression was inversely correlated with disease parameters of RA patients, such as rheumatoid factor, erythrocyte sedimentation rate, and C-reactive protein. In vitro, our data showed that human T lymphocytes released exosomes containing large amounts of miR-204-5p, which can be transferred into synovial fibroblasts, inhibiting cell proliferation. Overexpression of miR-204-5p in synovial fibroblasts suppressed synovial fibroblast activation by targeting genes related to cell proliferation and invasion. In vivo assays found that administration of lentiviruses expressing miR-204-5p markedly alleviated the disease progression of the mice with CIA. Collectively, this study identified a novel RA-associated plasma exosomal miRNA-204-5p that mediates the communication between immune cells and synovial fibroblasts and can be used as a potential biomarker for RA diagnosis and treatment.

Non-linear FEA of mechanical properties of modular prefabricated steel-concrete composite joints

Wu Cheng Long,Kan Jian Cheng,Liu Ji Ming,Mou Ben 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.40 No.4

This paper study the seismic performance of modular prefabricated SRC column to steel beam composite joint (MPCJ) under static loading. Numerical modeling of MPCJs with three different beam-end connections was carried out in ABAQUS. Results of the numerical calculation were compared with existing quasi-static test results to verify the feasibility of the numerical model. The model was then used to analyze changes in the moment-rotation relation, failure mode, ductility, stiffness, and bolt stress in the joint between the column and joint module. Besides, stress distributions in the joints were analyzed along different stress paths. Under monotonic loading, the mechanical performance of the MPCJ is significantly affected by the beam end connection mode. Failure of the MPCJ mainly occurs at the beam end connection, thus, the aim of keeping the plastic hinge away from the joint core and preventing shear failure is achieved. Furthermore, the maximum story drift ratio is 3.9–5 times greater than the recommended limit, which indicates good ductility and deformation performance. The MPCJ of the three-beam end connection methods is all semi-rigid connections. The beam end structure will have a large influence on the bolt tension and stress distribution. According to the test research results, a nonlinear model with three parameters including joint cover plate cantilever section length, flange connecting plate thickness and flange connecting plate weld length was established. The theoretical calculation results was consistent with the results of the numerical simulation. MPCJs can be designed based on the proposed theoretical calculation formula.

Enhancement of Polysialic Acid Yield by Reducing Initial Phosphate and Feeding Ammonia Water to Escherichia coli CCTCC M208088

Jian-Rong Wu,Jin-Long Liu,Xiao-Bei Zhan,Chi-Chung Lin,Hui Zhao 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.4

Polysialic acid (PSA) is a capsular polysaccharide obtained from aerobic fermentation with Escherichia coli. To enhance PSA production and eliminate the influence of phosphate on the PSA purification process, a lower level of initial phosphate was adopted with pH control. The resulting PSA yield reached 4.1 g/L in fed-batch fermentation with 2.5 g/L K2HPO4 and E. coli strain CCTCC M208088. In addition, an ammonia water (NH4OH) feeding strategy to control the pH at 6.4 was developed resulting in PSA production that reached as high as 5.2 g/L. NMR spectra confirmed the purified biopolymer as a α-2,8linked PSA, identical to the published NMR spectra, with a molecular weight in the range of 16 ~ 50 kDa.

Three New 29 Carbon Skeletons Pentacyclic Triterpenoids and S-equol from Biogas Slurry

Jian-Feng Xu,Hui-Bin Wu,Ding-Cai Liu,Long Sha,Wen-Hui Wu,Hua Fan,Yishan Song,Hong-Guang Zhu 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.12

Bioactive natural products were firstly obtained from biogas slurry. Three new 29 carbon skeletons of the pentacyclic triterpenoid compounds 24-norolean-12-ene-3,22-dione (1), 3β-hydroxy-24-norolean-12-ene-22-one (2), 3α-hydroxy-24-norolean-12-ene-22-one (3), as well as one known compound S-equol (4) were isolated and purified from the MeOH extract of chicken manure biogas slurry. The molecular structures of the four compounds were elucidated based on the extensive spectroscopic data analysis, and the structure of Compound 1 was further confirmed by single-crystal X-ray diffraction. The structures of Compounds 1, 2, and 3 are similar with oleanolicum and hederagenin that has excellent anti-tumor activities. The cytotoxicity against five cancer cell lines (Hela, A549, MCF7, PC3, and B16) of Compounds 1–4 was tested. Similar to Compound 4 (S-equol), Compounds 1–3 (pentacyclic triterpenoids) showed cytotoxicity activity against different tumor cell lines. Compounds 1–3 showed slightly lower cytotoxicity activities than Compound 4. The IC50 of Compound 4 was determined to be 9.7–27.6 μM, while the IC50 values of 1–3 were 17.6–65.3 μM. There are no significant differences in the cytotoxicity capacities between Compounds 1, 2, and 3.

FEM analysis of the modular prefabricated steel-concrete composite beam-column internal joint under reciprocating action

Cheng Long Wu,Jian Cheng Kan,Qi Hui Wang,Ji Ming Liu,Zun Qiang Li 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.41 No.1

Prefabricated steel-concrete composite structure has the advantages of large bearing capacity, reliable connection, and good durability, which is of great significance to the development of building industrialization. In this paper, a finite element model (FEM) of the modular prefabricated SRC column-steel beam composite joint is established and verified its accuracy through ABAQUS. The effects of various parameter variables on the hysteresis curve, skeleton curve, ductility, energy consumption, and performance deterioration are investigated. The results show that as the axial compression ratio increases, the joint bearing capacity first increases and then decreases, the strength and stiffness have relatively stable degradation characteristics. However, the hysteresis curve has a certain “pinch” phenomenon, which reduces the energy consumption performance. Different column end bolts margin and apertures have little effect on the bearing capacity, strength and stiffness degradation performance, but affect energy consumption and ductility. The width-to-thickness ratio of the square steel tube has a greater impact on the bearing capacity, energy consumption and ductility, and has less impact on the strength and stiffness degradation performance. As the thickness of the joint cover plate increases, the bearing capacity, energy consumption and initial stiffness of the joint gradually increase, the ductility first increases and then decreases.

An improved resonantly driven piezoelectric gas pump

Yue Wu,Yong Liu,Jian-Fang Liu,Long Wang,Xiaoyang Jiao,Zhigang Yang 대한기계학회 2013 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.27 No.3

Piezoelectric pumps have the potential to be used in a variety of applications, such as in air circulation and compression. However, piezoelectric membrane pumps do not have enough driving capacity, and the heat induced during the direct contact between the driving part and the gas medium cannot be dissipated smoothly. When the gas is blocked, the piezoelectric vibrator generates heat quickly, which may eventually lead to damage. Resonantly driven piezoelectric stack pumps have high performance but no price advantage. In this situation, a novel, resonantly driven piezoelectric gas pump with annular bimorph as the driver is presented. In the study, the working principle of the novel pump was analyzed, the vibration mechanics model was deter-mined, and the displacement amplified theory was studied. The outcome indicates that the displacement amplification factor is related with the original displacement provided by the piezoelectric bimorph. In addition, the displacement amplification effect is related to the stiffness of the spring lamination, adjustment spring, and piezoelectric vibrator, as well as to the systematic damping factor and the driving frequency. The experimental prototypes of the proposed pump were designed, and the displacement amplification effect and gas output performance were measured. At 70 V of sinusoidal AC driving voltage, the improved pump amplified the piezoelectric vibrator displacement by 4.2 times, the maximum gas output flow rate reached 1685 ml/min, and the temperature of the bimorph remained normal after 2000 hours of operation when the gas medium was blocked.

Prevalence of Spina Bifida Occulta and Its Relationship With Overactive Bladder in Middle-Aged and Elderly Chinese People

Jun Wei Wu,Yu Rong Xing,Yi Bo Wen,Tian Fang Li,Quan De Feng,Xiao Ping Shang,Yun Long Li,Jin Jin Feng,Xin Xin Wang,Rong Qun Zhai,Xiang Fei He,Tao Chen,Xin Jian Liu,Jian Guo Wen 대한배뇨장애요실금학회 2016 International Neurourology Journal Vol.20 No.2

Purpose: To investigate the prevalence of spina bifida occulta (SBO) and its relationship with the presence of overactive bladder (OAB) in middle-aged and elderly people in China. Methods: A cross-sectional community-based survey was carried out at 7 communities in Zhengzhou City, China from December 15, 2013 to June 10, 2014, where residents aged over 40 years were randomly selected to participate. All of the participants underwent lumbosacral radiographic analysis and relevant laboratory tests. A questionnaire including basic information, past medical history and present illness, and the OAB symptom score was filled out by all participants. Chi-square tests and logistic regression were used for data analysis with a P-value of <0.05 denoting statistical significance. Results: A total of 1,061 subjects were qualified for the final statistical analysis (58.8±11.7 years; male, 471 [44.4%]; female, 590 [55.6%]). The overall prevalence of SBO was 15.1% (160 of 1,061): 18.3% (86 of 471) in men and 12.5% (74 of 590) in women. Among these subjects, 13.7% (145 of 1,061) had OAB: 13.2% (62 of 471) in men and 14.1% (83 of 590) in women. The results of logistic regression showed that age, SBO, history of cerebral infarction (HCI), and constipation were risk factors for OAB (P<0.05), while sex, history of childhood enuresis (HCE), body mass index (BMI), and diabetes mellitus (DM) were not (P>0.05). In men, age, SBO, and constipation were risk factors for OAB (P<0.05), while HCE, BMI, DM, HCI, and benign prostate hyperplasia were not (P>0.05). In women, age, SBO, and HCI were risk factors for OAB (P<0.05), while HCE, BMI, DM, vaginal delivery, and constipation were not (P>0.05). Conclusions: The prevalence of SBO is high and it is related to OAB in middle-aged and elderly people in China.

Heparanase mRNA and Protein Expression Correlates with Clinicopathologic Features of Gastric Cancer Patients: a Meta-analysis

Li, Hai-Long,Gu, Jing,Wu, Jian-Jun,Ma, Chun-Lin,Yang, Ya-Li,Wang, Hu-Ping,Wang, Jing,Wang, Yong,Chen, Che,Wu, Hong-Yan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.18

Background: Heparanase is believed to be involved in gastric carcinogenesis. However, the clinicopathologic features of gastric cancer with high heparanase expression remain unclear. Aim : The purpose of this study was to comprehensively and quantitatively summarize available evidence for the use of heparanase mRNA and protein expression to evaluate the clinicopathological associations in gastric cancer in Asian patients by meta-analysis. Materials and Methods: Relevant articles listed in MEDLINE, CNKI and the Cochrane Library databases up to MARCH 2015 were searched by use of several keywords in electronic databases. A meta-analysis was performed to clarify the impact of heparanase mRNA and protein on clinicopathological parameters in gastric cancer. Combined ORs with 95%CIs were calculated by Revman 5.0, and publication bias testing was performed by stata12.0. Results: A total of 27 studies which included 3,891 gastric cancer patients were combined in the final analysis. When stratifying the studies by the pathological variables of heparanase mRNA expression, the depth of invasion (633 patients) (OR=4.96; 95% CI=2.38-1.37; P<0.0001), lymph node metastasis (639 patients) (OR=6.22; 95%CI=2.70-14.34, P<0.0001), and lymph node metastasis (383 patients) (OR=6.85; 95% CI=2.04-23.04; P=0.002) were all significant. When stratifying the studies by the pathological variables of heparanase protein expression, this was the case for depth of invasion (1250 patients) (OR=2.76; 95% CI=1.52-5.03; P=0.0009), lymph node metastasis (1178 patients) (OR=4.79 ; 95% CI=3.37-6.80, P<0.00001), tumor size (727 patients) (OR=2.06 ; 95% CI=1.31-3.23; P=0.002) (OR=2.61; 95% CI=2.09-3.27; P=0.000), and TNM stage (1233 patients) (OR=6.85; 95% CI=2.04-23.04; P=0.002). Egger's tests suggested publication bias for depth of invasion, lymph node metastasis, lymph node metastasis and tumor size of heparanase mRNA and protein expression. Conclusions: This meta-analysis suggests that higher heparanase expression in gastric cancer is associated with clinicopathologic features of depth of invasion, lymph node metastasis and TNM stage at mRNA and protein levels, and of tumor size only at the protein level. Egger's tests suggested publication bias for these clinicopathologic features of heparanase mRNA and protein expression, and which may be caused by shortage of relevant studies. As a result, although abundant reports showed heparanase may be associated with clinicopathologic features in gastric cancer, this meta-analysis indicates that more strict studies were needed to evaluate its clinicopathologic significance.

Klotho May Ameliorate Proteinuria by Targeting TRPC6 Channels in Podocytes

Kim, Ji-Hee,Xie, Jian,Hwang, Kyu-Hee,Wu, Yueh-Lin,Oliver, Noelynn,Eom, Minseob,Park, Kyu-Sang,Barrezueta, Nestor,Kong, In-Deok,Fracasso, R. Paul,Huang, Chou-Long,Cha, Seung-Kuy American Society of Nephrology 2017 Journal of the American Society of Nephrology Vol.28 No.1