제주재래돼지와 듀록 참조축군에서 Melanocortin 4 Receptor (MC4R) 유전자형과 지방산 조성간의 관련성 분석

강용준(Yong-Jun Kang),김상금(Sang-Geum Kim),김수연(Su-Yeon Kim),신문철(Moon-Cheol Shin),우제훈(Jae-Hoon Woo),김남영(Nam-Young Kim),신상민(Sang-Min Shin),최재영(Jae-Young Choi),유지현(Ji-Hyun Yoo),박남건(Nam-Geon Park),양병철(Byoung-C 한국생명과학회 2020 생명과학회지 Vol.30 No.1

본 연구에서는 제주재래돼지와 듀록 품종 사이에서 생산된 F₂ 참조축군에서 melanocortin-4-receptor (MC4R) 유전자형과 지방산 조성간의 관련성을 연구하였다. 전체 290 여두의 F₂ 자손을 이용하여 14개의 지방산 조성을 측정하였다. Taq Ⅰ PCR-RFLP 방법을 이용하여 MC4R c.1426A>G (p.Asp298Asn)의 단일염기다형성(single nucleotide polymorphism, SNP)을 확인하였다. MC4R 세 가지 유전자형(AA, AB, BB)이 모두 발견되었고, 그 빈도는 각각 0.299, 0.542, 0.159로 확인되었다. AA 유전자형을 가진 개체에서 palmitic acid (C16:0, p<0.05), stearic acid (C18:0, p<0.01), eicosenoic acid (C20:1n9, p<0.05), saturated fatty acid (SFA, p<0.01) 함량이 GG 유전자형을 가진 개체보다 더 높은 것으로 확인되었다. 반면에 GG 유전자형을 가진 개체는 linoleic acid (C18:2n6, p<0.001), linolenic acid (C18:3n3, p<0.001), linolenic acid (C18:3n6, p<0.001), arachidonic acid (C20:4n6, p<0.001)과 같은 불포화지방산(unsaturated fatty acid) 함량이 AA 유전자형을 가진 개체보다 더 높은 것을 확인하였다. 제주재래돼지와 듀록 품종 사이에서 생산된 F₂ 참조축군에서 MC4R GG 유전자형이 포화지방산은 낮추고, 불포화지방산은 높이는 것으로 확인되었다. 본 연구 결과 MC4R의 유전적 다형성이 듀록과 제주재래돼지 교배 프로그램에서 육질향상과, 고기내의 지방산 함량을 조절할 수 있는 유전적 표지 인자로 활용될 수 있을 것이라 사료된다. This study was conducted to examine association between melanocortin-4-receptor (MC4R) genotypes and fatty acid (FA) composition in an F₂ intercross between Duroc and Jeju (South Korea) Native pigs (JNP). Fourteen FA composition traits were measured in more than 290 F₂ progeny population produced between Duroc and JNP. All experimental pigs were successfully genotyped for the MC4R c.1426A>G (p.Asp298Asn) single nucleotide polymorphism (SNP) by using Taq Ⅰ PCR-RFLP methods. We detected three MC4R genotypes, AA, AG, and GG with 0.299, 0.542, and 0.159 genotype frequencies, respectively. The MC4R AA genotype animals showed higher levels in palmitic acid (C16:0, p<0.05), stearic acid (C18:0, p<0.01), eicosenoic acid (C20:1n9, p<0.05), saturated fatty acid (SFA, p<0.01) than GG homozygotes, respectively. Whereas MC4R GG genotype showed higher values in linoleic acid (C18:2n6, p<0.001), linolenic acid (C18:3n3, p<0.001), linolenic acid (C18:3n6, p<0.001), arachidonic acid (C20:4n6, p<0.001) and unsaturated fatty acid (USFA, p<0.01) than AA pigs, respectively. The MC4R GG genotype was associated with increasing USFA and decreasing SFA in the F₂ progeny population produced between Duroc and JNP. Our finding suggests that the MC4R polymorphisms can be used as a valuable genetic marker for Duroc and JNP breeding programs to improve meat quality and to control FA compositions.

14.6 A GeV ^28Si 중이온이 원자핵건판내에서 발생시킨 핵반응에서 생성된 2차입자의 발생각 분포

김종오,김태연,남신우,신택수,우종관,이세병,임계엽,장세덕,조재희,천병구,임인택,김기영 慶尙大學校 기초과학연구소 1990 基礎科學硏究所報 Vol.6 No.-

14.6A GeV^28Si 중이온이 원자핵 건판내에서 발생시킨 N_h=1인 핵반응에서 생성된 47개의 파쇄 α 입자와 537개의 단일하전 2차입자의 발생각들을 측정하여 변수 exp(γ-η_b)의 포괄적 분포를 회귀함수 dN=exp[a+χ{exp(γ-η_b)d{exp(γ-η_b)}로 적합시켰다. 여기서 의사신속도 γ=arctanh(cosθ)=-ln tan(θ/2)이고, 입사 중이온의 신속도 η_b=3.445이다. 그 적합결과 파쇄 α입자의 경우 χ=-0.052±0.011이고, 파쇄 p입자의 경우 χ=-0.141±0.015이었다. For LS emission angles of 47 α fragments and 537 single-charged shower particles, produced by the N_h (the number of heavyprongs)=1 interactions of 14.6 A GeV^28Si nuclei in the nuclear emulsion, the distribution of the parameter exp(γ-η_b) is well expressed by dN=exp[a+χ{exp(γ-η_b)d{exp(γ-η_b)}with χ=-0.052±0.011 for αfragments and χ=-0.141±0.015 for p 'fragments', where the pseudorapidity of secondaries γ=arctanh(cosθ)=-ln tan(θ/2) and the rapidity of incident heavy ions, η_b=3.445.

Enhanced rate capability of LiMn_0.9Mg_0.1PO₄ nanoplates by reduced graphene oxide/carbon double coating for Li-ion batteries

Wi, S.,Kim, J.,Nam, S.,Kang, J.,Lee, S.,Woo, H.,Lee, M.,Sonu, C.H.,Moon, T.,Park, B. Elsevier 2014 Current Applied Physics Vol.14 No.5

The reduced graphene oxide (RGO)/carbon double-coated LiMn<SUB>0.9</SUB>Mg<SUB>0.1</SUB>PO<SUB>4</SUB> (LMP) nanoplates are introduced as a cathode material for Li-ion batteries with excellent rate capability. The double coating of RGO and carbon simultaneously brings the unique advantages of conformal carbon layer on each particle surface, and soft RGO sheets connecting the nanoplates to each other, thereby provides easy conduction pathways for the whole LMP aggregates. In particular, the simple self-assembly process driven by the electrostatic interactions enables conducting RGO sheets effectively to wrap the carbon-coated LMP, establishing three-dimensional RGO network. The RGO/C/LMP nanocomposites exhibit remarkably enhanced rate capability compared to the only C- or RGO-coated LMP, which is well explained by the reduced charge-transfer resistance achieved from electrochemical impedance spectroscopy.

LTCC를 이용한 MCM VCO 제작에 관한 연구

이영신,곽승범,유찬세,이우성,강남기,박종철 한국마이크로전자및패키징학회 2000 추계 기술심포지움 논문집 Vol.2 No.1

레이저흡광분석기(Laser Absorption Spectrometry)를 이용한 CO₂가스의 탄소안정동위원소비 보정식 산출

정태양,우남칠,신우진,봉연식,최승현,김연태,Jeong, Taeyang,Woo, Nam C.,Shin, Woo-Jin,Bong, Yeon-Sik,Choi, Seunghyun,Kim, Youn-Tae 대한자원환경지질학회 2017 자원환경지질 Vol.50 No.6

Stable carbon isotope ratio of carbon dioxide (${\delta}^{13}C_{CO2}$) is used as an important indicator in the researches for global climate change and carbon capture and sequestration technology. The ${\delta}^{13}C$ value has been usually analyzed with Isotope Ratio Mass Spectrometer (IRMS). Recently, the use of Laser Absorption Spectrometry (LAS) is increasing because of the cost efficiency and field applicability. The purpose of this study was to suggest practical procedures to prepare laboratory reference gases for ${\delta}^{13}C_{CO2}$ analysis using LAS. $CO_2$ gas was adjusted to have the concentrations within the analytical range. Then, the concentration of $CO_2$ was assessed in a lab approved by the Korea Laboratory Accreditation Scheme and the ${\delta}^{13}C_{CO2}$ value was measured by IRMS. When the instrument ran over 12 hours, the ${\delta}^{13}C$ values were drifted up to ${\pm}10$‰ if the concentration of $CO_2$ was shifted up to 1.0% of relative standard deviation. Therefore, periodical investigation of analytical suitability and correction should be conducted. Because ${\delta}^{13}C_{CO2}$ showed the dependency on $CO_2$ concentration, we suggested the equation for calibrating the concentration effect. After calibration, ${\delta}^{13}C_{CO2}$ was well matched with the result of IRMS within ${\pm}0.52$‰. 기후변화 연구 및 이산화탄소($CO_2$) 지중저장 분야 등에서 $CO_2$ 가스의 탄소안정동위원소비(${\delta}^{13}C_{CO2}$)는 주요한 인자로서 인식되고 있다. ${\delta}^{13}C$는 주로 안정동위원소질량분석기(Isotope Ratio Mass Spectrometry: IRMS)로 분석하지만, 최근 분석비용 및 현장적용성의 문제로 레이저흡광분석기(Laser Absorption Spectrometry: LAS)의 사용이 확대되고 있다. 본 연구는 LAS를 이용한 ${\delta}^{13}C_{CO2}$ 분석 시 분석적합성을 판단하는데 필요한 표준가스의 실질적 확보 방안과 주의사항을 제시하는 것을 목표로 하였다. 실험실 표준가스는 분석할 농도범위로 $CO_2$ 가스를 조제한 후, 한국인정기구 인증시험기관에서 $CO_2$ 농도를 측정한 후에 IRMS로 ${\delta}^{13}C_{CO2}$를 측정하여 사용하였다. 장시간 측정 시, 농도가 상대표준편차 1.0% 이하로 변이하면 ${\delta}^{13}C$는 최대 ${\pm}10$‰까지 변동할 수 있으므로, 표준가스를 주기적으로 측정하여 분석 적합성을 판단하고 필요 시 보정을 하여야 한다. ${\delta}^{13}C_{CO2}$는 $CO_2$ 농도에 의존성을 나타내므로, 분석하고자 하는 영역의 최소 및 최대 농도를 갖는 표준가스의 혼합실험을 통해 보정식을 산출하였으며, 보정 후 ${\delta}^{13}C_{CO2}$는 IRMS 측정값에서 ${\pm}0.52$‰ 이내의 편차를 나타내었다.

Quantitative analysis of hydrocephalic ventricular alterations in Yorkshire terriers using magnetic resonance imaging

Woo, D-C,Choi, C-B,Nam, J-W,Ryu, K-N,Jahng, G-H,Lee, S-H,Lee, D-W,Kim, S-Y,Kim, H-Y,Ahn, K-J,Choe, B-Y Czech Academy of Agricultural Sciences 2010 VETERINARNI MEDICINA -PRAHA- Vol.55 No.3

<P>The purpose of this work was to evaluate hydrocephalic ventricular changes using three quantitative analysis methods. The height, area and volume of the ventricles and brain were measured in 20 Yorkshire terriers (10 normal and 10 hydrocephalic dogs) using low-field MR imaging (at 0.2 Tesla). All measurements were averaged and the relative ventricle size was defined as a percentage (percent size of the ventricle/size of the brain). The difference between normal and hydrocephalic dogs was statistically significant for the average of each ventricle as well as for the percentage value. Five hydrocephalic symptoms were identified: circling, head tilting, seizures, ataxia, and strabismus. With respect to height, area and volume of the brain/ventricle, the difference between normal and hydrocephalic dogs was not significant. The ventricle/brain with height (1D) was related to the area (2D) and volume (3D). The correlations with area and volume were as good as the ventricle/brain height ratio in the case of hydrocephalic dogs. Therefore, one-, two- and three-dimensional quantitative methods may be complementary. We expect that the stage of hydrocephalic symptoms can be classified if statistical significance for ventricular size among symptoms is determined with the analysis of a large number of hydrocephalic cases.</P>

Hydrogeochemical Changes in the Groundwater System at the Gyeongju LILW Disposal Site After Construction

Nam C. Woo,Eunhye Kwon,Jae-Min Lee,Youn-Tae Kim,Sang Min Ryu,Soon-Il Ok 한국방사성폐기물학회 2017 한국방사성폐기물학회 학술논문요약집 Vol.2017 No.05

논문 : 지하수 모니터링 이상변동 자료를 이용한 소규모 지진 영향 유추

우남칠 ( Nam C Woo ),( Jize Piao ),이재민 ( Jae Min Lee ),이찬진 ( Chan Jin Lee ),강인옥 ( In Oak Kang ),최두형 ( Doo Houng Choi ) 대한지질공학회 2015 지질공학 Vol.25 No.1

이 연구는 국내에서 운용되고 있는 지하수 장기관측망의 자동 관측자료를 이용하여, 발생빈도가 상대적으로 높은 M3.0내외의 소규모 지진에 의한 영향과 지진 전조현상에 의한 지하수의 변화 관측 및 구분가능성을 평가하고자 수행되었다. 사용된 지하수 관측자료는 2012년 4월~6월 기간의 1시간 단위로 김천지좌, 강진성전, 공주정안 3개 관측소의 암반관측공에서 관측된 자료이다, 지하수위, 수온 및 EC 값의 시계열 자료에서 부분적으로 급격한 이상변동이 관측되었으며, 이변동은 2012년 5월 30일 경북 영덕에서 발생한 M3.1 지진의 전조와 그 영향으로 해석된다. 그럼에도 불구하고 지진의규모 및 발생위치와 관측공까지의 거리와 지하수 변동의 크기는 선형관계를 보이지 않으며, 이는 관측공 자체의 구조적 특성과도 연관된다. 따라서 지진관측을 고유목적으로 하는 관측공의 설치, 운용이 필요하며, 이를 통한 장기적 모니터링은 지진 재난지역에서의 비상수자원 확보와 지하공간 안전성 확보의 기반정보를 제공할 수 있을 것이다. This study tests the potential of detecting small-magnitude earthquakes (~M3.0) and their precursors using a long-term groundwater-monitoring database. In groundwater records from April to June 2012, abnormal changes in water level, temperature, and electrical conductivity were identified in the bedrock monitoring wells of the Gimcheon- Jijwa, Gangjin-Seongjeon, and Gongju-Jeongan stations. These anomalies could be attributed to the M3.1 earthquake that occurred in the Youngdeok area on May 30th, although no linear relationship was found between the scale of changes and the distance between each monitoring station and the epicenter, which is attributed in part to the wide screen design of the monitoring wells. Groundwater monitoring networks designed specifically for monitoring earthquake impacts could provide better information on the safety of underground space and on the security of emergency water-resources in earthquake disaster areas.

2016 경주 지진에 따른 지하수의 반응

우남칠(Nam C. Woo),이재민(Jaemin Lee),조선주(Sunju Cho),권은혜(Eunhye Kwon) 대한지질학회 2016 대한지질학회 학술대회 Vol.2016 No.10

Smart Reset Control for Wide-Dynamic-Range LWIR FPAs

Woo, D H,Nam, I K,Lee, H C IEEE 2011 IEEE SENSORS JOURNAL Vol.11 No.1

<P>A new readout circuit involving a pixel-level reset control was studied for 2-D long-wavelength infrared (LWIR) focal plane arrays. The integration time of each pixel can be optimized individually and automatically. Hence, the readout circuit has a wide dynamic range and good signal-to-noise ratio (SNR) characteristics. The readout circuit was fabricated with a 0.35-μm 2-poly 4-metal CMOS process for a 128 × 128 LWIR HgCdTe array with a pixel size of 50 μm × 50 μm. The smart reset control with two-step background suppression improves the SNR to 87 dB and the dynamic range to 95.8 dB.</P>