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- 저자 : Wesam Al Khateeb (검색결과 1 건)
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Wesam Al Khateeb,Razan Kanaan,Tamam El-Elimat,Muhammad Alu’datt,Jamil Lahham,Ahmad El-Oqlah 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.4
Lavenders (Lavandula species) are important aromatic ornamental medicinal plants with wide rangingapplications in perfume and pharmaceutical industries. We developed an in vitro propagation protocol for Lavandulacoronopifolia. Murashige and Skoog (MS) medium supplemented with 0.5 mg・L-1N6-benzyladenine was the bestmedium for the proliferation of microshoots, while the highest rooting frequency was obtained using MS mediumsupplemented with 1.0 mg・L-1indole-3-butyric acid. Inter-simple sequence repeat analysis revealed that the invitro-propagated microshoots were highly genetically stable, even after subculture. The highest callus fresh weight(667.9 mg) was obtained by propagating on medium supplemented with a combination of 1.0 mg・L-1naphthaleneaceticacid and 0.5 mg・L-1butyric acid. Using the Folin-Ciocalteu method, methanolic extracts of wild L. coronopifoliarevealed total phenolic content of 4.9 mg expressed in gallic acid equivalents (GAE) (mg GAE・g-1dry matter). Radicalscavenging activity was estimated at 85% using the free radical 2,2-diphyenyl-picrylhydrazyl assay. Using the brineshrimp assay for cytotoxicity, the methanolic extract was found to be nontoxic. Finally, liquid chromatography tandemmass spectrometry with standard reference compounds was used to quantify the key phenolic compounds in both invitro and in vivo-grown L. coronopifolia. Six major phenolic compounds were identified: caffeic acid, rosmarinic acid,rutin, quercetin, luteolin, and hesperidin. Levels of these phenolic compounds were highest in wild plant extracts.
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