Simpler Efficient Group Signature Scheme with Verifier-Local Revocation from Lattices

( Yanhua Zhang ),( Yupu Hu ),( Wen Gao ),( Mingming Jiang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.1

Verifier-local revocation (VLR) seems to be the most flexible revocation approaches for any group signature scheme, because it just only requires the verifiers to possess some up-to-date revocation information, but not the signers. Langlois et al. (PKC 2014) proposed the first VLR group signature based on lattice assumptions in the random oracle model. Their scheme has at least O(n²)·logN bit group public key and O (n) ·log N bit signature, respectively. Here, n is the security parameter and N is the maximum number of group members. In this paper, we present a simpler lattice-based VLR group signature, which is more efficient by a(log)ONfactor in both the group public key and the signature size. The security of our VLR group signature can be reduced to the hardness of learning with errors (LWE) and small integer solution (SIS) in the random oracle model.

Quantitatively Decoupling the Impact of Preload and Internal Mechanism Motion on Pyrotechnic Separation Shock

Shihui Xiong,Yanhua Li,Yaokun Ye,Jingcheng Wang,Huina Mu,Yuquan Wen 한국항공우주학회 2021 International Journal of Aeronautical and Space Sc Vol.22 No.5

Pyrotechnic separation devices are widely used in space separation systems. However, these devices produce high-frequency, high-amplitude shock responses during operation, which endangers the safety of space systems. Hence, it is imperative to study the shock mechanism and characteristics during the unlocking process of a pyrotechnic separation device. This paper uses LS-DYNA finite element software and experiments to analyze the separation characteristics of the separation nut and predict its shock response, quantitatively decoupling the impact of preload and internal mechanism motion on separation shock. The results indicate that the shock response caused by the impact on the inner sleeve is the primary contributor to the total shock response of the separation nut (approximately 55% of the total response). The shock response caused by the release of the strain energy is very small, accounting for less than 5% of the total shock response. Adjusting the preload force without changing the charge and structure of the separation device did not have a clear impact on reducing the shock. This study shows that the shock of the separation device can be decreased by reducing the impact on the inner sleeve.

Rational Design and Molecular Engineering of Peptide Aptamers to Target Human Pancreatic Trypsin in Acute Pancreatitis

Weiyi Shao,Wenxian Zhu,Yanhua Wang,Jingwei Lu,Ge Jin,Yixin Wang,Wenli Su 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.1

Human pancreatic trypsin (hPT) is an established target for acute pancreatitis (AP) therapeutics. Here, a bioinformatics protocol of protein docking, peptide refinement, dynamics simulation and affinity analysis was described to perform rational design and molecular engineering of hPT peptide aptamers. Protein docking was employed to model the intermolecular interactions between hPT and its cognate inhibitory protein, the human pancreatic trypsin inhibitor (hTI). A number of peptide fragments were cut out from the interaction sites of docked hPT–hTI complexes, from which a decapeptide fragment 13LNGCTLEYRP22 was found to exhibit potent inhibition against hPT (Ki = 5.3 ± 0.8 μM). We also carried out alanine scanning and virtual mutagenesis to systematically examine the independent contribution of peptide residues to binding affinity, and the harvested knowledge were then used to guide modification and optimization of the decapeptide fragment. Subsequently, inhibition studies of nine promising candidates against recombinant hPT were conducted, from which four samples were successfully identified to have high or moderate potency (Ki < 10 μM). In particular, the peptides LQVCTLEYCN and LQICTLEYCT were found to inhibit hPT activity significantly (Ki = 0.23 ± 0.04 and 0.85 ± 0.18 μM, respectively). Structural analysis of hPT– peptide complex systems unraveled diverse chemical interactions such as hydrogen bonds, salt bridges and hydrophobic forces across the complex interfaces.

mTOR Signal Transduction Pathways Contribute to TN-C FNIII A1 Overexpression by Mechanical Stress in Osteosarcoma Cells

Zheng, Lianhe,Zhang, Dianzhong,Zhang, Yunfei,Wen, Yanhua,Wang, Yucai Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.2

Osteosarcoma is the most common primary malignant bone tumor with a very poor prognosis. Treating osteosarcoma remains a challenge due to its high transitivity. Tenascin-C, with large molecular weight variants including different combinations of its alternative spliced FNIII repeats, is specifically over expressed in tumor tissues. This study examined the expression of Tenascin-C FNIIIA1 in osteosarcoma tissues, and estimated the effect of mechanical stimulation on A1 expression in MG-63 cells. Through immunohistochemical analysis, we found that the A1 protein was expressed at a higher level in osteosarcoma tissues than in adjacent normal tissues. By cell migration assay, we observed that there was a significant correlation between A1 expression and MG-63 cell migration. The relation is that Tenascin-C FNIIIA1 can promote MG-63 cell migration. According to our further study into the effect of mechanical stimulation on A1 expression in MG-63 cells, the mRNA and protein levels of A1 were significantly up-regulated under mechanical stress with the mTOR molecule proving indispensable. Meanwhile, 4E-BP1 and S6K1 (downstream molecule of mTOR) are necessary for A1 normal expression in MG-63 cells whether or not mechanical stress has been encountered. We found that Tenascin-C FNIIIA1 is over-expressed in osteosar-coma tissues and can promote MG-63 cell migration. Furthermore, mechanical stress can facilitate MG-63 cell migration though facilitating A1 overexpression with the necessary molecules (mTOR, 4E-BP1 and S6K1). In con-clusion, high expression of A1 may promote the meta-stasis of osteosarcoma by facilitating MG-63 cell migration. Tenascin-C FNIIIA1 could be used as an indicator in metastatic osteosarcoma patients.

Composite group vector space method for estimating melting and boiling point of pure organic compound

Wei Wenying,Wang Zhen,Yin Yanhua,Han Jinyu,Xu Wen 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.3

Composition Group Vector Space (CGVS) method for estimating melting and boiling point Tm, Tb of organic compound has been proposed, and the principle of this method has been elucidated. The models for estimating Tm, Tb have been established and the numerical values of relative parameters have been presented. The average percentage deviations of Tm, Tb estimation are 7.53 and 1.58, respectively, which show that the present method demonstrates significant improvement in applicability to predict the above properties, compared to conventional group methods

mTOR Signal Transduction Pathways Contribute to TN-C FNIII A1 Overexpression by Mechanical Stress in Osteosarcoma Cells

Lianhe Zheng,Dianzhong Zhang,Yunfei Zhang,Yanhua Wen,Yucai Wang 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.2

Osteosarcoma is the most common primary malignant bone tumor with a very poor prognosis. Treating osteosarcoma remains a challenge due to its high transitivity. Tenascin-C, with large molecular weight variants including different combinations of its alternative spliced FNIII repeats, is specifically over expressed in tumor tissues. This study examined the expression of Tenascin-C FNIIIA1 in osteosarcoma tissues, and estimated the effect of mechanical stimulation on A1 expression in MG-63 cells. Through immunohistochemical analysis, we found that the A1 protein was expressed at a higher level in osteosar-coma tissues than in adjacent normal tissues. By cell migration assay, we observed that there was a significant correlation between A1 expression and MG-63 cell migra-tion. The relation is that Tenascin-C FNIIIA1 can promote MG-63 cell migration. According to our further study into the effect of mechanical stimulation on A1 expression in MG-63 cells, the mRNA and protein levels of A1 were significantly up-regulated under mechanical stress with the mTOR molecule proving indispensable. Meanwhile, 4E-BP1 and S6K1 (downstream molecule of mTOR) are necessary for A1 normal expression in MG-63 cells whether or not mechanical stress has been encountered. We found that Tenascin-C FNIIIA1 is over-expressed in osteosar-coma tissues and can promote MG-63 cell migration. Furthermore, mechanical stress can facilitate MG-63 cell migration though facilitating A1 overexpression with the necessary molecules (mTOR, 4E-BP1 and S6K1). In con-clusion, high expression of A1 may promote the meta-stasis of osteosarcoma by facilitating MG-63 cell migration. Tenascin-C FNIIIA1 could be used as an indicator in metastatic osteosarcoma patients.