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Negotiating Competence with Multiple-Yeah in Academic Contexts
( Hansun Zhang Waring ) 서강대학교 언어정보연구소 2012 언어와 정보 사회 Vol.16 No.-
Hansun Zhang Waring(2012), "Negotiating Competence with Multiple- Yeah in Academic Contexts," Language & Information Society 16. Previous work on "yeah" has shown that it can be used as a continuer, to claim agreement (Schegloff 1982), to indicate speakership incipiency (Jefferson 1993), or to withhold treating the prior as informative (Heritage 1984). On the other hand, Stivers (2004) has shown that multiple sayings (e.g., "No no no," "wait wait wait," etc.) are used to express one`s stance that the prior speaker has persisted unnecessarily and should properly halt his/her course of action. Using the methodology of conversation analysis, the current paper extends this body of research by accounting for the use of multiple-yeah in academic contexts, and in particular, its use as a resource for managing competence negotiation in graduate peer-tutoring and graduate seminar discussions. Findings of this study also contribute to the work on talk and identity, and in particular, on competence negotiation in academic settings.
Utilization of Putrescine by Streptococcus pneumoniae During Growth in Choline-limited Medium
Ware D.,Watt J.,Swiatlo E. The Microbiological Society of Korea 2005 The journal of microbiology Vol.43 No.5
Polyamines such as putrescine are small, ubiquitous polycationic molecules that are required for optimal growth of eukaryotic and prokaryotic cells. These molecules have diverse effects on cell physiology and their intracellular content is regulated by de novo synthesis and uptake from the environment. The studies presented here examined the structure of a putative polyamine transporter (Pot) operon in Streptococcus pneumoniae (pneumococcus) and growth of pneumococci in medium containing putrescine substituted for choline. RT-PCR experiments demonstrated that the four genes encoding the Pot system are co-transcribed with murB, a gene involved in an intermediary step of peptidoglycan synthesis. Pneumococci grown in chemically-defined media (CDM) containing putrescine without choline enter logarithmic phase growth after 36-48 hs. However, culture density at stationary phase eventually reaches that of choline-containing medium. Cells grown in CDM-putrescine formed abnormally elongated chains in which the daughter cells failed to separate and the choline-binding protein PspA was no longer cell-associated. Experiments with CDM containing radiolabeled putrescine demonstrated that pneumococci concentrate this polyamine in cell walls. These data suggest that pneumococci can replicate without choline if putrescine is available and this polyamine may substitute for aminoalcohols in the cell wall teichoic acids.
Utilization of Putrescine by Streptococcus pneumoniae During Growth in Choline-limited Medium
D. Ware,J. Watt,E. Swiatlo 한국미생물학회 2005 The journal of microbiology Vol.43 No.5
Polyamines such as putrescine are small, ubiquitous polycationic molecules that are required for optimal growth of eukaryotic and prokaryotic cells. These molecules have diverse effects on cell physiology and their intracellular content is regulated by de novo synthesis and uptake from the environment. The studies presented here examined the structure of a putative polyamine transporter (Pot) operon in Streptococcus pneumoniae (pneumococcus) and growth of pneumococci in medium containing putrescine substituted for choline. RT-PCR experiments demonstrated that the four genes encoding the Pot system are co-transcribed with murB, a gene involved in an intermediary step of peptidoglycan synthesis. Pneumococci grown in hemically-defined media (CDM) containing putrescine without choline enter logarithmic phase growth after 36-48 hs. However, culture density at stationary phase eventually reaches that of choline-containing medium. Cells grown in CDM-putrescine formed abnormally elongated chains in which the daughter cells failed to separate and the choline-binding protein PspA was no longer cell-associated. Experiments with CDM containing radiolabeled putrescine demonstrated that pneumococci concentrate this polyamine in cell walls. These data suggest that pneumococci can replicate without choline if putrescine is available and this polyamine may substitute for aminoalcohols in the cell wall teichoic acids.
Evaluation of Neutron Cross Sections for Hafnium in the Resolved Resonance Range
T. Ware,D. Weaver,M. Moxon,C. Dean,R. Hiles,P. Schillebeeckx,S. Kopecky 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.23
The international High Priority Request list notes: - "In the nuclear industry hafnium is used as neutron absorbing material to regulate the fission process. Interpretation of critical experiments with UOx fuel conducted by CEA in the AZUR zero-power reactors has shown systematic underestimation of the reactivity worth that may be attributed to an overestimated natural hafnium capture cross section in the epi-thermal energy range"To service the request for improved resonance data a PhD project has:-a) Improved REFIT R-matrix evaluation code.b) Obtained hafnium oxide samples enriched in Hf176, 177, 178, 179 isotopes.c) Gained support from NUDAME and EUFRAT projects.d) Prepared enriched and natural Hf samples.e) Performed capture and transmission Time of Flight measurements at the GELINA linear accelerator.f) Analysed the capture counts to generate yields using AGS and AGL codes.g) Used REFIT to perform least squares analysis of GELINA measurements.(Included previous ORNL, Harwell and RPI transmissions and capture yields.)h) Submitted results to EXFOR.i) Included resolved resonance parameters in JEFF evaluations taking the resolved range to over 1keV. j) Tested evaluations with suitable benchmarks.k) Passed resolved resonance data to CEA Cadarache for unresolved analysis.Resultant Hf evaluations will be included in JEFF3.2.
Development of integrated waste management options for irradiated graphite
Alan Wareing,Liam Abrahamsen-Mills,Linda Fowler,Michael Grave,Richard Jarvis,Martin Metcalfe,Simon Norris,Anthony William Banford 한국원자력학회 2017 Nuclear Engineering and Technology Vol.49 No.5
The European Treatment and Disposal of Irradiated Graphite and other Carbonaceous Waste projectsought to develop best practices in the retrieval, treatment, and disposal of irradiated graphite includingother irradiated carbonaceous waste such as structural material made of graphite, nongraphitized carbonbricks, and fuel coatings. Emphasis was given on legacy irradiated graphite, as this represents a significantinventory in respective national waste management programs. This paper provides an overview ofthe characteristics of graphite irradiated during its use, primarily as a moderator material, within nuclearreactors. It describes the potential techniques applicable to the retrieval, treatment, recycling/reuse, anddisposal of these graphite wastes. Considering the lifecycle of nuclear graphite, from manufacture to finaldisposal, a number of waste management options have been developed. These options consider thetechniques and technologies required to address each stage of the lifecycle, such as segregation, treatment,recycle, and ultimate disposal in a radioactive waste repository, providing a toolbox to aid operatorsand regulators to determine the most appropriate management strategy. It is noted that nationalwaste management programs currently have, or are in the process of developing, respective approachesto irradiated graphite management. The output of the Treatment and Disposal of Irradiated Graphite andother Carbonaceous Waste project is intended to aid these considerations, rather than dictate them.
Akshay P. Ware,Faiyaz K. Shaikh,Archana N. Panche,Sanjay N. Harke 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.1
Gut proteases are accountable for survival of Helicoverpa armigera on protein rich parts of plant devastating many important agricultural crops. The aim of present study was to identify potential natural compounds having inhibitory potency against Helicoverpa armigera gut proteases. We have modeled structure of H. armigera serine protease (UniProt ID: O18447) and analyzed its interactions with maslinic acid (Zinc ID: ZINC38140521). A 3D model was generated using bovine trypsin in complex with analogues of sunflower inhibitor 1 as template with the help of Chimera Modeler 1.11. The PROCHECK and Modfold analysis have revealed 81.8% of residue in favored region. The POOL and COACH analysis have revealed 18 amino acids in the active site. In the 10 ns MD simulations of modeled structure, the RMSD of the protein backbone increased slightly and later stabilized from 7 ns to 10 ns. The modeled structure was stabilized at gyration distance of about 1.65 nm at 7 ns. Potential hit compounds from the ZINC database identified in this study showed good inhibitory bindings with modeled structure. Among these compounds maslinic acid, a plant based pentacyclic triterpenes was found to be potent lead compound with good binding affinity (−9.5 kcal/mol). RMSD profile was < 0.45 nm for complex with stabilization at about 18,000 ps (18 nm) suggesting stable interaction. This work demonstrates reasonable in silico inhibitory action of maslinic acid against H. armigera serine protease and depicts utility of in silico methodologies for designing competent strategies against dreaded insect pests like H. armigera.