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Role of the Meckel’s Cartilage in Embryonic Mandibular Development of Mice
J. W Choi,S. B Han,J. H Sung,H. I Shin 대한구강악안면병리학회 2005 대한구강악안면병리학회지 Vol.29 No.5
Mecke!'s car t ilage is one of the ea rliest structu re to appear in a mandible derived from the lï rst branchi a l a rch and serves as the primorclium I"or the formation 01‘ mandible‘ mall eus. incus. and sphenomandibular li gament However. its direct role a nd the mechanism in mandibular clevelopment a re not well elucidated. 1'0 address t his Issue‘ we observed morphol ogical and histological changes and gene expression patterns in the Mecke!'s cart ilage 01" a cleveloping mouse. I"rom E13.5 to E18.5 embryos. using skeletal preparation samples a ncl routinely prepa red s lide secti ons for light mi croscopic observation in various sectional planes. The following methods were per |‘ ormecl : H&E staining I"or general hi st이 og i cal observation ‘ Von Kossafor detection of minerali zation. TRAP activ ity staining for locali zaLion 01’ osteoclastic cell s. immunohistochemistry for !Iα@-1 a ncl -9 forevaluati on of enzy matic activity 01" osteoclasLic cell s. a ncl in situ hybricli zation for detection of collagen type 1. Il. ancl X mRNA ex presslon‘ respecLively. AL E1 3.5 Mec kel's cartilage appeared as a V-shaped rod fused a t the micl line and thin minera li zed ma ndibular buccal plaLe was I"ormed lateral to. and at some clistance from. Meckel’s carti lage in an intramembranous ossi lïcation mocle. WiLh the progression of tooth development. t he Meckel’s in carti lage adjacent incisors revealecl hyperLrophi c chonclrocyte di ff"er entiation with minerali zation of the chondroid matrix. The Meckel’s car Li lage was replacecl with bone by o~ L eoc l asLs . showing strong immunoreact ivity for MMP- l ancl -9 from E16 5 Wi Lh ti me‘ Lhis bony replacement of Meckel's cartilage in an endochondral ossification mode was ex Lenclecl up Lo the mid-porLion of Lhe molar sockets til l EI8.5. The bony replacement of minera li zed hypertrophic chondrocyte zone expressing X collagen mHNA conLri buted to the formation of thick mandibular lingual plate . 1'hese f"i ndings suggesL LhaL mandibular formalion and development is closely relatecl with not only Mecke!'s carLi lage. buL also wiLh Lhc developing LooLh. and thaL C'erLai n in f"l uence from the developing tooth may play a role in detcrmin in g Lhe faLc of Meckel’s ca rLi lage cluring ma ndi bular development.
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축산물 및 가공 : 상업적 응유효소의 탈지유에 대한 단백질 분해 작용
신현수 ( H. S. Shin ),김상범 ( S. B. Kim ),임종우 ( J. W. Lim ) 한국축산학회 2002 한국축산학회지 Vol.44 No.6
Proteolytic activities of some commercial milk clotting enzymes(rennet, trypsin, pepsin, papain W-40, neutrase 1.5 and protease S) in bovine skim milk containing 0.02% CaCl_2 were determined by measuring DH(Degree of Hydrolysis), NPN(Non Protein Nitrogen) and by comparing patterns of SDS-PAGE(Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis). The DH of microbial enzymes(neutrase 1.5 and protease S) and trypsin in bovine skim milk were higher than those of pepsin and papain W-40. The amounts of NPN in the milk treated with trypsin and the other animal enzymes(rennet and pepsin) showed the highest and lowest degrees of proteolysis, respectively. SDS-PAGE showed that trypsin and protease S hydrolyzed α-lactalbumin and papain W-40 hydrolyzed β-lactoglobulin slightly, while neutrase 1.5 hydrolyzed both α-lactalbumin and β-lactoglobulin after treating for 90 min. Trypsin and protease S easily hydrolyzed α_s-casein and β-casein, which were not hydrolyzed by rennet. Papain W-40 hydrolyzed k-casein more than rennet as shown in SDS-PAGE. Based on the results of the experiments, the DH and NPN of trypsin, neutrase 1.5 and protease S were shown to be higher than those of the other enzymes. The SDS-PAGE patterns of papain W-40 and neutrase 1.5 were similar with that of rennet.
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Park, Y.K.,Nho, S.W.,Shin, G.W.,Park, S.B.,Jang, H.B.,Cha, I.S.,Ha, M.A.,Kim, Y.R.,Dalvi, R.S.,Kang, B.J.,Jung, T.S. Elsevier Scientific Pub. Co 2009 Veterinary microbiology Vol.136 No.1
The rates of antibiotic susceptibility and resistance were investigated in Streptococcus iniae and Streptococcus parauberis isolates obtained from diseased olive flounders (Paralichthys olivaceus) collected from fish farms in Jeju Island, Korea. Isolates of S. iniae (n=65) were susceptible to cefotaxime, erythromycin, ofloxacin, penicillin, tetracycline and vancomycin, as demonstrated by the minimum inhibitory concentration (MIC) test. Isolates of S. parauberis (n=86) were highly resistant to erythromycin (58% of the 86 isolates tested) and tetracycline (63% of the 86 isolates tested). Fifty-four isolates of tetracycline-resistant S. parauberis contained the tet(M/O/S) genes, of which 39 and 12 isolates contained the tet(M) and tet(S) genes, respectively, whereas 3 isolates contained both the tet(M) and tet(S) genes. Among the erythromycin-resistant isolates of S. parauberis (n=50) only 14 contained the erm(B) gene. These results suggest that the tet(S) and erm(B) genes of S. parauberis are involved in the acquisition of high-level resistance to erythromycin and tetracycline. Our findings reveal a high rate of antibiotic resistance among strains of S. parauberis and emphasize the need to develop an appropriate vaccine to reduce the use of antibiotics.
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권세원(S. W. Kwon),박용범(Y. B. Park),권신원(S. W. Kwon),권용무(Y. M. Kwon),김점식(J. S. Kim),송현석(H. S. Song),문성원(M. S. Mun),신운용(W. Y. Shin) 한국조명·전기설비학회 2006 한국조명·전기설비학회 학술대회논문집 Vol.2006 No.11월
기존의 인력굴착을 통한 지선근가 매설시공방법을 오거크레인에 의한 기계화시공을 하기 위하여 오거크레인 굴착공에 적합한 원형 지선근가를 개발을 하고 개발된 원형근가를 오거크레인을 이용한 기계화시공에 적용함으로써 공사시간 및 비용 절감과 시공품질의 향상을 이루려 한다.
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The effect of gp130 on Meckel's Ca에 lage Morphogenesis and Subsequent Mandibular Development
J W Choi,J T Kim,J Y chof,s. Y Kim,EK Park,H I Shin 대한구강악안면병리학회 2005 대한구강악안면병리학회지 Vol.29 No.5
gp130-Mediated signaling is involved in both chondrogenesis and osteogenesis. but its direct role in the mecha nism of embryonic Meckel’s cartilage and associated mandibular development has not yet been elucidated . In this s tudy. we examined the influence of gp130 ablation on the morphogenesis of Meckel’s cartilage and subsequent d evelopment of mandible by evaluating the morphological and histological changes as well as the gene expression patterns in developing embryonic gp130 deficient Ullce The abla tion of the gp130 gene showecl 110 change in region- specific collagen mRNA expression except for a s light delay in its expression but caused shortened embry 。nic Mecke]'s cartilage, delayed hypertrophic chondrocyte maturation and subseqllent bony replacement with characteristic bending of the intramandibular Meckel's cartilage. The bending 0 1' Meckel's cartilage leads to a narrow mandibular arch at the rostral area with poor cortical plate formation. These fir띠 ings indi cate that gp130 is im p ortant for the normal morphogen않is 01' Meckel's cartiJage and sllbsequent mandib띠a‘ c1evelopmen
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허학영(Hag Young Heo),M. Hockings,신원우(W. W. Shin),정혁진(H. J. Chung),N. Dudley,P. Shadie,R. Vaisanen,G. Vincent,김현(H. Kim),박소영(S. Y. Park),양성우(S. W. Yang) 국립공원연구원 2010 국립공원연구지 Vol.1 No.3
생물다양성협약(CBD) 제7차 당사국총회에서 채택된 보호지역실행프로그램(PoWPA)은 당사국들에게 그들의 보호지역에 대한 관리효과성 평가 수행을 권고하고 있다. 이 연구의 목적은 생물다양성협약의 권고사항 이행과 더불어 한국 보호지역시스템에 대한 진단 및 관리 개선을 위한 권고사항을 도출하고자 하였다. 평가 대상지는 환경부에서 관할하고 있는 국립공원, 생태경관보전지역, 습지보호지역, 야생동식물특별보호지역 총 39개소(6,898 km2)를 대상으로 하였다. 평가는 2가지 차원에서 수행되었는데 39개 보호지역(한국 보호지역의 면적 대비 40%정도)에 대한 개별 평가와 한국보호지역시스템에 대한 평가가 이뤄졌으며, 시스템 평가는 국제전문가들과 함께 현장조사, 관리자 및 이해관계자인터뷰, 문헌검토 등을 통해 수행되었다. 평가 결과 개별 보호지역이 국제적으로 잘 관리되고 있었으며, 거버넌스와 행정부분이 다른 나라에 비해 상대적으로 강점으로 나타났고, 생물다양성보호와 자연자연관리에 대한 조화와 협력 부분이 상대적으로 미흡한 것으로 나타났다. 또한 비전과 계획 부분이 특히 강하며, 전반적인 국민의 높은 지지가 매우 인상적이지만, 다른 여러 나라들과 마찬가지로 지역사회협력 부분이 약한 것으로 나타났다. 보호지역의 관리를 개선하기 위한 10가지 주요 권고사항은 보전을 위한 지역적 접근 강화, 보호지역시스템계획 강화, 기관간 통합적 관리 개선, 지역사회와 협력 개선, 이해관계자와 협력 강화, 지방정부와의 협력 개선, 인력관리 재고, 재원 다양화, 연구과제 다양화, 자연자원과 문화자원의 관리 조화 개선 등이다. The Convention on Biological Diversity (CBD)’s Programme of Work on Protected Areas (PoWPA), which was adopted in CoP-7 in 2004, calls on countries to undertake assessments of the effectiveness of management of their protected areas (PA). The objectives of this study are to implement a recommendation of PoWPA, to review the current management status of PA system, and to find out some recommendations for the better management of protected areas in Korea. Assessment Sites are 39 protected areas (6,898 km2) managed under Ministry of Environment which are National parks, Ecosystem & landscape conservation areas, Wetland protected areas, and Wildlife special protected areas. The assessment was conducted at two levels: a site assessment of 39 protected areas, representing about 40% by area of the Korean PA system and a system-level assessment conducted by an international review team incorporating field visits, interviews with staff and stakeholders and review of relevant documentation. The assessment results generally show that individual protected areas are well managed on any international comparison. Governance and administration appears to be relatively stronger in Korea than in other countries, however harmonization and coordination of biodiversity protection and natural resource management remains relatively weaker. Korea also appears to be particularly strong in vision and planning. Korea’s trend toward increasingly high levels of overall public approval is globally impressive; however, as is the case in the rest of the world, community engagement at local levels remains weak and problematic. Ten key recommendations for improving management were to develop a more regional approach to conservation, strengthen PA system planning, better integrate management across agencies within Korea, improve local community relations, enhance interaction with stakeholders, improve integration with regional government, review staff management practices, diversify the funding base, broaden the focus of internal research work and better harmonize natural and cultural resource management.
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