Kdr allelic variation in a sodium channel gene from a population of South Carolina Heliothis virescens (Fabricius)

Junmo Cho,Sujin Park,Chunkeun Lim,Yong Chul Park,Jang Hyun Hur,Soonsung Hong,Thomas M. Brown,Saeyoull Cho 한국응용곤충학회 2008 Journal of Asia-Pacific Entomology Vol.11 No.3

Mutations at V421M and L1029H in the hscp sodium channel gene are known to contribute to knockdown resistance (kdr) in the Woodrow, Dalzell, and PTJ strains of H. virescens (tobacco budworm) from the cotton fields of South Carolina, USA. In the IS6 region of the sodium channel gene, the frequencies of the mutant allele methionine in the Woodrow and Dalzell strains were 0.07 and 0.1, respectively. For the IIS6 region, the frequencies of the mutant allele histidine in Woodrow and Dalzell strains were 0.175 and 0.263, respectively. In the PTJ strain, the frequencies of methionine and histidine alleles were 0 and 0.1, respectively. The Hpy3 allele, which is strongly linked to the histidine mutant allele, was also found in Woodrow and Dalzell strains. In addition, we found a new allele, which is one nucleotide different from Hpy3, called Hpy3-1, and found that it is also linked to histidine. Mutations at V421M and L1029H in the hscp sodium channel gene are known to contribute to knockdown resistance (kdr) in the Woodrow, Dalzell, and PTJ strains of H. virescens (tobacco budworm) from the cotton fields of South Carolina, USA. In the IS6 region of the sodium channel gene, the frequencies of the mutant allele methionine in the Woodrow and Dalzell strains were 0.07 and 0.1, respectively. For the IIS6 region, the frequencies of the mutant allele histidine in Woodrow and Dalzell strains were 0.175 and 0.263, respectively. In the PTJ strain, the frequencies of methionine and histidine alleles were 0 and 0.1, respectively. The Hpy3 allele, which is strongly linked to the histidine mutant allele, was also found in Woodrow and Dalzell strains. In addition, we found a new allele, which is one nucleotide different from Hpy3, called Hpy3-1, and found that it is also linked to histidine.

Development of Efficient Dynamic Aeroelasticity Model for High Fidelity Pointing Accuracy Assessment of VLBI Earth-Based Radio Antennas

Nieto Michelle Guzman,Thomas Paul V.,ElSayed Mostafa S. A.,Saad Mohamed,Brown Gary L.,Hilliard Lawrence M. 한국항공우주학회 2020 International Journal of Aeronautical and Space Sc Vol.21 No.3

Modern Earth-based radio antennas of very-long-baseline interferometry system are furnished with robust control systems for their pointing control. Their pointing accuracy is critical to the quality of the radio wave-front captured. External disturbances, particularly those of wind gusts, produce a non-negligible dynamic aeroelastic response that degrades its pointing accuracy, and yet are not mitigated by the antenna’s control system. In this paper, a high fidelity and efficient dynamic aeroelastic model of an earth-based antenna is developed which is used to study the effects of wind gusts on the antenna’s pointing accuracy. Model order reduction of the antenna structural model is carried out using Craig–Bampton method taking into consideration the dominant modal characteristics of the antenna. The aerodynamic forces are approximated using the 2D Doublet-Lattice Method. The Davenport Spectrum is used to model aerodynamic turbulences near the earth surface. The developed dynamic aeroelastic model is employed to investigate the effects of discrete and random gusts on the pointing accuracy of the antenna. It is found that the deviation in the pointing angle is more prominent in the z (azimuth) direction and it displays a quadratic dependency with respect to the mean wind speed. This behavior is attributed to the inertial component of the aeroelastic response solution represented by the gravitational field acting on the center of gravity of the main reflector and the counterweights. The developed efficient aeroelastic model can be integrated into the antenna control system for its response prediction and mitigation.

Low versus standard central venous pressure during laparoscopic liver resection: A systematic review, meta-analysis and trial sequential analysis

Mina Stephanos,Christopher M. B. Stewart,Ameen Mahmood,Christopher Brown,Shahin Hajibandeh,Shahab Hajibandeh,Thomas Satyadas 한국간담췌외과학회 2024 Annals of hepato-biliary-pancreatic surgery Vol.28 No.2

To compare the outcomes of low central venous pressure (CVP) to standard CVP during laparoscopic liver resection. The study design was a systematic review following the PRISMA statement standards. The available literature was searched to identify all studies comparing low CVP with standard CVP in patients undergoing laparoscopic liver resection. The outcomes included intraoperative blood loss (primary outcome), need for blood transfusion, mean arterial pressure, operative time, Pringle time, and total complications. Random- effects modelling was applied for analyses. Type I and type II errors were assessed by trial sequential analysis (TSA). A total of 8 studies including 682 patients were included (low CVP group, 342; standard CVP group, 340). Low CVP reduced intraoperative blood loss during laparoscopic liver resection (mean difference [MD], –193.49 mL; 95% confidence interval [CI], –339.86 to –47.12; p = 0.01). However, low CVP did not have any effect on blood transfusion requirement (odds ratio [OR], 0.54; 95% CI, 0.28–1.03; p = 0.06), mean arterial pressure (MD, –1.55 mm Hg; 95% CI, –3.85–0.75; p = 0.19), Pringle time (MD, –0.99 minutes; 95% CI, –5.82–3.84; p = 0.69), operative time (MD, –16.38 minutes; 95% CI, –36.68–3.39; p = 0.11), or total complications (OR, 1.92; 95% CI, 0.97–3.80; p = 0.06). TSA suggested that the meta-analysis for the primary outcome was not subject to type I or II errors. Low CVP may reduce intraoperative blood loss during laparoscopic liver resection (moderate certainty); however, this may not translate into shorter operative time, shorter Pringle time, or less need for blood transfusion. Randomized controlled trials with larger sample sizes will provide more robust evidence.

Utilization of cryopreserved sperm cells based on the indication for storage

Graham Luke Machen,Stephanie E. Harris,Erin T. Bird,Monica L. Brown,Dale A. Ingalsbe,Milaida M. East,Michelle Reyes,Thomas J. Kuehl 대한비뇨의학회 2018 Investigative and Clinical Urology Vol.59 No.3

Purpose: Cryopreservation of sperm from human semen has been available since the 1950s. The actual utilization of available cryopreservation technology has been infrequently reported. We set out to examine the utilization and outcomes of cryopreserved sperm cells based on the indication for storage. Materials and Methods: A dataset was developed from retrospective review. The purposes for cryopreservation, eventual utilization, and outcomes of use for insemination were recorded. The types of utilization were accumulated as proportions for different purposes. The timing for use of samples for insemination procedures was evaluated using survival statistics. The frequency of patients arranging to destroy samples was also reported. Results: From September 1988 through March 2015, 1442 samples were cryopreserved. Samples were cryopreserved for four primary purposes: infertility treatments focused on intrauterine insemination (IUI) or in vitro fertilization/intracellular injection (IVF/ICSI), for fertility preservation related to cancer treatment, or prior to military deployment. Total utilization rates were 19.3%. Samples cryopreserved for IUI were more likely to be used (64.3%), while samples cryopreserved as backup for IVF/ICSI were more likely to be destroyed (29.8%). Pregnancy rates varied based on the indication and ART used. Pregnancies per cycle were 35% for IVF/ICSI and were 10% for IUI. Conclusions: Cryopreservation of sperm is a valuable and underutilized resource, particularly amongst male cancer patients. This technology can facilitate infertility treatments based on a variety of indications, including deployment—a patient cohort unique to our dataset.

Function biomedical informatics research network recommendations for prospective multicenter functional MRI studies

Glover, Gary H.,Mueller, Bryon A.,Turner, Jessica A.,van Erp, Theo G.M.,Liu, Thomas T.,Greve, Douglas N.,Voyvodic, James T.,Rasmussen, Jerod,Brown, Gregory G.,Keator, David B.,Calhoun, Vince D.,Lee, H Wiley Subscription Services, Inc., A Wiley Company 2012 JOURNAL OF MAGNETIC RESONANCE IMAGING Vol.36 No.1

<P><B>Abstract</B></P><P>This report provides practical recommendations for the design and execution of multicenter functional MRI (MC‐fMRI) studies based on the collective experience of the Function Biomedical Informatics Research Network (FBIRN). The study was inspired by many requests from the fMRI community to FBIRN group members for advice on how to conduct MC‐fMRI studies. The introduction briefly discusses the advantages and complexities of MC‐fMRI studies. Prerequisites for MC‐fMRI studies are addressed before delving into the practical aspects of carefully and efficiently setting up a MC‐fMRI study. Practical multisite aspects include: (i) establishing and verifying scan parameters including scanner types and magnetic fields, (ii) establishing and monitoring of a scanner quality program, (iii) developing task paradigms and scan session documentation, (iv) establishing clinical and scanner training to ensure consistency over time, (v) developing means for uploading, storing, and monitoring of imaging and other data, (vi) the use of a traveling fMRI expert, and (vii) collectively analyzing imaging data and disseminating results. We conclude that when MC‐fMRI studies are organized well with careful attention to unification of hardware, software and procedural aspects, the process can be a highly effective means for accessing a desired participant demographics while accelerating scientific discovery. J. Magn. Reson. Imaging 2012;36:39–54. © 2012 Wiley Periodicals, Inc.</P>