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Consumer response tendency and its implications for satisfaction and recommendation intention
Takumi Kato 한국마케팅과학회 2022 마케팅과학연구 Vol.32 No.2
It is important to understand customer satisfaction and loyalty from the perspective of business and services. However, when compar- ing these indicators among multiple services, it is doubtful whether superiority or inferiority in customer satisfaction and loyalty is due to different levels of service quality. In other words, there is a possibility that consumer response tendency may differ depend- ing on the service and the attributes of the respondent. Few aca- demic studies address response tendency in this context. This study filled this gap in the literature and clarify response tendency by service type, which has not been sufficiently addressed until now. This study evaluates the response tendencies of satisfaction and recommendation intention by service and respondent using an integrated survey covering 20 types of services. As a result of one- way analysis of variance, a high score was confirmed for the car sharing and streaming industries, for which consumer recommen- dations are incorporated into services, and the moving industry scored highly due to a high level of transparency in operations. Additionally, the younger generation, who habitually post recom- mendations on social networks, showed a significantly higher recommendation intention. This study shows that understanding respond tendency in advance and excluding bias from data pro- vides truer results.
Dosimetric properties of Mg-doped Al2O3 transparent ceramics
Takumi Kato,Noriaki Kawaguchi,Takayuki Yanagida 한양대학교 세라믹연구소 2019 Journal of Ceramic Processing Research Vol.20 No.5
Transparent ceramics of Al2O3 doped with different concentrations of Mg2+ ions (0.001, 0.01, 0.1 and 1.0%) were synthesized by the Spark Plasma Sintering (SPS) method, and scintillation and dosimetric properties were investigated. Under X-ray irradiation, all the samples showed emission peaks around 310, 380 and 693 nm. Based on the measured scintillation decay times and previous studies, the origins of the emission peaks at 310, 380 and 693 nm were attributed to F+ centers, F centers and Cr3+ impurity ions, respectively. The 0.1% Mg-doped sample showed a glow curve consisting of peaks around 50, 105, 135, 230, 350 and 400 oC. All the samples had a linear TSL response in a dose range from 0.1 to 1,000 mGy.
Radio-photoluminescence properties of CaF2 transparent and opaque ceramics
Kato Takumi,Nakauchi Daisuke,Kawaguchi Noriaki,Yanagida Takayuki 한국물리학회 2020 Current Applied Physics Vol.20 No.11
Radio-photoluminescence (RPL) phenomenon was observed in CaF2 transparent and opaque ceramics by X-ray irradiation. The RPL appeared as a formation of photoluminescence (PL) emission bands located around 660 and 720 nm caused by F2+ and (F2+)A centers, respectively. The RPL signal can be deleted by heating, and reusability was observed over ten experimental cycles. The fading of the RPL signal was confirmed to be ~4% in 10 days. The detectable RPL response of CaF2 transparent ceramics was as low as 1 mGy, and the dynamic ranges were confirmed over 1–10000 mGy with linear response.
Paramagnetic-assisted NMR Analyses of Conformational Dynamics of Gangliosides
Ying Zhang,Takumi Yamaguchi,Sayoko Yamamoto,Yoshinori Uekusa,Koichi Kato 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
For better understanding the molecular basis of the mechanism underlying oligosaccharides functions, it is quite desirable to gain detail information on their conformational dynamics in solution. However, the conformational analysis of oligosaccharides is still a remaining challenge, because of their branched covalent structures and dynamic conformational multiplicities, which hinder conventional analytical methods. For conformational characterization of flexible oligosaccharides, their structures should not be dealt with as a single well-defined global free energy minimum but as an ensemble of low energy conformers. Hence, we have been developing a methodology for evaluating a dynamic ensemble of oligosaccharide conformations by employing paramagnetic-assisted NMR methods based on paramagnetic effects such as pseudocontact shift (PCS) and paramagnetic relaxation enhancement (PRE) in conjunction with molecular dynamics (MD) simulations [1,2]. We applied this approach to the conformational dynamics analyses of branched GM2 tetrasaccharide, βGalNAc-(1-4)-[αNeu5Ac-(2-3)]-βGal-(1-4)-βGlc, which share the common core structure of gangliosides forming an integral part of cellular membranes. We synthesized the GM2 tetrasaccharide covalently attached to a lanthanide chelating-tag (Fig. 1). Upon complexation with paramagnetic lanthanide ions, the tagged sugar exhibited NMR spectral changes due to PCSs. The observed PCS values were in excellent agreement with those back-calculated from the 3D ensemble models derived from the MD calculations. In addition, we have developed spin-labeling method of gangliosides for their NMR analyses in amphipathic environments. These methodologies open a new prospect for conformational analyses of dynamic structures of gangliosides toward decoding glycocodes from the 3D structural aspects.
Kotaro Yanagi,Yukiko Kamiya,Toshihiko Kitajima,Takumi Yamaguchi,Yasunori Chiba,Koichi Kato 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
High-mannose-type oligosaccharides are enzymatically trimmed in the endoplasmic reticulum, giving rise to various processing intermediates with exposure of specific glycotopes that are recognized by a series of lectins involved in glycoprotein-fate determination in cells. Atomic information of dynamic oligosaccharide conformations is essential for a quantitative understanding of energetics of the carbohydrate-lectin interactions. Although carbohydrate NMR spectroscopy is useful for characterizing such conformational dynamics, it is often hampered by poor spectral resolution and the lack of recombinant technique to produce homogeneous glycoforms. To overcome these difficulties, we have recently developed a methodology for preparation of a homogeneous high-mannose-type oligosaccharide with 13C labeling using genetically engineered yeast strain. We herein successfully extended this method to overexpression of 13C-lebeled Man9GlcNAc2 (M9) using a newly engineered yeast strain with deletion of four genes involved in N-glycan processing. This enabled high-field NMR analyses of 13C-labeld M9 in comparison with its processing product M8B, which lacks the terminal mannose residue ManD2. Long-range NOE data indicated that the outer branches can interact with the core in both glycoforms and such foldback conformations are enhanced upon the removal of ManD2. The observed conformational variabilities might be associated with the lectins and the glycan-trimming enzymes.