Identification of Proteins Differentially Expressed in the Striatum by Melatonin in a Middle Cerebral Artery Occlusion Rat Model—a Proteomic and <i>in silico</i> Approach

Shah, Fawad Ali,Zeb, Amir,Ali, Tahir,Muhammad, Tahir,Faheem, Muhammad,Alam, Sayed Ibrar,Saeed, Kamran,Koh, Phil-Ok,Lee, Keun Woo,Kim, Myeong Ok Frontiers Media S.A. 2018 Frontiers in neuroscience Vol.12 No.-

<P>Ischemic stroke is characterized by permanent or transient obstruction of blood flow, which initiates a cascading pathological process, starting from acute ATP loss to subsequent membrane depolarization, glutamate excitotoxicity, and calcium overload. Melatonin is a potent antioxidant that exerts protective effects in different experimental stroke models. In this study, melatonin effects were demonstrated by a proteomic and <I>in silico</I> approach. The proteomic study identified differentially expressed proteins by 2D gel electrophoresis in the striatum 24 h after middle cerebral artery occlusion. Proteomic analysis revealed several proteins with aberrant expression and was validated by western blot and immunofluorescence analysis. Homology modeling was performed to build 3D structures for γ-enolase, thioredoxin (TRX), and heat shock 60 (HSP60) by the template crystal structures using a protein data bank as a sequence database. The structure refinement of each model was achieved by energy minimization via molecular dynamic simulation, and the generated models were further assessed for stability by Procheck and ProSA. The models were processed for docking analysis using AutoDock Vina, and post-docking analysis was determined by discovery studio. The proteomic study showed decreased expression of γ-enolase, TRX, and protein phosphatase 2A subunit B and increased expression of collapsin response mediator protein 2 and HSP60 in the striatum after ischemic injury. Treatment with melatonin modulated the expression profiles of these proteins. This study demonstrated the neuroprotective role of melatonin in the ischemic striatum using a proteomic and <I>in silico</I> approach. Collectively, melatonin may act in a multimechanistic way by modulating the expression of several proteins in the ischemic striatum.</P>

LATTICE ORDERED FUZZY SOFT GROUPS

( Tahir Mahmood ),( Naveed Ahmad Shah ) 호남수학회 2018 호남수학학술지 Vol.40 No.3

In several _elds the soft set theory has a very vast range uses and applications. A soft group is a proper family of subgroups and a fuzzy soft group is a proper family of fuzzy subgroups. Here in this paper the concept of lattice ordered fuzzy soft groups is introduced. Also its some properties are studied and discussed. In addition, the de_ntion of lattice order fuzzy soft right (left) cosets and lattice order soft product of fuzzy soft subgroups and some related results are discussed to clear these ideas.

LATTICE ORDERED FUZZY SOFT GROUPS

Mahmood, Tahir,Shah, Naveed Ahmad The Honam Mathematical Society 2018 호남수학학술지 Vol.40 No.3

In several fields the soft set theory has a very vast range uses and applications. A soft group is a proper family of subgroups and a fuzzy soft group is a proper family of fuzzy subgroups. Here in this paper the concept of lattice ordered fuzzy soft groups is introduced. Also its some properties are studied and discussed. In addition, the defintion of lattice order fuzzy soft right (left) cosets and lattice order soft product of fuzzy soft subgroups and some related results are discussed to clear these ideas.

Performance evaluation of novel solarpowered domestic air cooler with Peltier modules

Zafar Abbas,A. N. Shah,M. Tahir Hassan,M. Sarfraz Ali,Qamar ud Din,Bilal Naseem,Ammar Asghar,Ali Haider 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.11

Shifting of air conditioning and ventilation plants from conventional to renewable energy systems is one of the effective ways to save energy and attain sustainability. In this experimental study, an effort has been made to design, fabricate, and evaluate the cooling performance of a solar-powered domestic air cooler with Peltier plates to meet the comfort criteria as per ASHRAE standards. It also investigates the effect of Peltier modules on the performance of conventional room air cooler. The experiments were conducted in a 12x12 room with one, two, three, and four Peltier modules operating at various ambient temperatures. The experiments were repeated three times at specified conditions. Peltier effect was used to decrease water temperature, and subsequently cooled water was used to decrease the temperature of the air after coming in contact with this water. The cooled air was then used to create a comfort zone. The results indicated a decrease in temperature of the experimental zone by 5 %, 13 %, 19 %, and 23 % using one, two, three, and four Peltier modules respectively. The increase in relative humidity was recorded as 5 % at 27 °C temperature of the experimental zone. The results of energy analysis showed a substantial amount of energy savings in this study and suggest that more than 200 MW energy can be saved by replacing conventional electric air coolers all over the country with proposed Peltier based domestic solar powered air cooler.

Neuroprotection by vitamin C against ethanol -induced neuroinflammation associated neurodegeneration in developing rat brain

Ahmad, Ashfaq,A. Shah, Shahid,Badshah, Haroon,J. Kim, Min,Ali, Tahir,H. Yoon, Gwang,H. Kim, Tae,B. Abid, Nouman,Ur Rehman, Shafiq,Khan, Sohail,O. Kim, Myeong Bentham Science 2016 CNS & Neurological Disorders Vol.15 No.3

Outcomes of Large Volume Paracentesis in Cirrhotic Patients with Spontaneous Bacterial Peritonitis

( Hafiz Abdul Basit Siddiqui ),( Muhammad Tahir Khan ),( Hasnain Ali Shah ),( Rabeea Azmat ),( Saad Bin Zafar ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims & Background: Bacterial infections are one of the most frequent complication in cirrhosis, particularly in patients with decompensated cirrhosis and accounts for significant mortality. Ascites is one of the most common complications of hepatic cirrhosis (1). In a country like Pakistan where the prevalence of Hepatitis C is on the rise, end stage liver disease is becoming a common scenario (2-4). Spontaneous bacterial peritonitis (SBP) occurs in 10-30% of such patients and is associated with high mortality rate among hospitalized patients (1, 5-7). The diagnosis of SBP through paracentesis is crucial in the management of SBP. SBP should be diagnosed early as for successful treatment of patients. Diagnostic paracentesis facilitates rapid initiation of antimicrobial therapy and permits optimal coverage in case a causative organism identified. However, more recently, the role of paracentesis in management of SBP is also explored through large volume removal of ascitic fluid. Despite the proposed risk of renal failure, Large Volume Paracentesis (LVP) among SBP patients has shown promising results indicated by lesser morbidity and mortality (8). Rationale of the study: Spontaneous bacterial peritonitis is a serious infectious condition with grave outcome in cirrhotic patients. Large volume paracentesis decreases the burden of infective fluid causing further deterioration of condition. Outcomes of LVP in SBP patients are not clearly addressed in previous studies. Furthermore, to date, no study from the country has reported the effect of large volume paracentesis on the management of SBP in hospitalized patients. This study will assess the outcomes of LVP in patients with SBP, both in terms of length of stay, in hospital 6 week mortality. Objectives: To compare the treatment outcomes among patients with and without large volume paracentesis diagnosed with Spontaneous Bacterial Peritonitis Methods: Study design: Analytical Cross-sectional Study Setting: Gastroenterology Unit, The Aga Khan University Hospital, Karachi Study Duration: 2 Years Study population: Patients admitted with Spontaneous Bacterial Peritonitis to Gastroenterology Unit, the Aga Khan University Hospital Sample Size: Using OpenEpi.com online sample size calculator and using the differences in hospital stay among patients with and without delayed paracentesis as 36.84% and 21.48% (8) the sample size was calculated to be 200 at 95% confidence level (100 in each group). Sampling Technique: Consecutive Sampling Inclusion Criteria: Patients admitted with ascites found to have spontaneous bacterial peritonitis on diagnostic paracentesis. 1) Patients with complete records 2) Above 18 and below 75 years of age Exclusion criteria: 1) Incomplete records 2) Secondary peritonitis Data Collection: The data will be extracted from the records and information analyzed will be patient age, gender, whether the patients receive large volume paracentesis or not, the length of hospital stay, and length of ICU stay. No patient will be enrolled for the study and retrospective data analysis will be carried out. Conclusions: Extracted data till now favours that LVP in patients with SBP translates into significantly positive outcomes in terms of length of hospital stay, spcecial care unit stay, need for terlipressin infusions, in hosptial mortality and 6 weeks mortality post large volume paracentesis. Hence LVP shoiuld be performed in patients suffering from SBP.

Aberrant Promoter Methylation at CpG Cytosines Induce the Upregulation of the E2F5 Gene in Breast Cancer

Arshad Ali,Farman Ullah,Irum Sabir Ali,Ahmad Faraz,Mumtaz Khan,Syed Tahir Ali Shah,Nawab Ali,Muhammad Saeed 한국유방암학회 2016 Journal of breast cancer Vol.19 No.2

Purpose: The promoter methylation status of cell cycle regulatory genes plays a crucial role in the regulation of the eukaryotic cell cycle. CpG cytosines are actively subjected to methylation during tumorigenesis, resulting in gain/loss of function. E2F5 gene has growth repressive activities; various studies suggest its involvement in tumorigenesis. This study aims to investigate the epigenetic regulation of E2F5 in breast cancer to better understand tumor biology. Methods: The promoter methylation status of 50 breast tumor tissues and adjacent normal control tissues was analyzed. mRNA expression was determined using SYBR® green quantitative polymerase chain reaction (PCR), and methylation- specific PCR was performed for bisulfite-modified genomic DNA using E2F5-specific primers to assess promoter methylation. Data was statistically analyzed. Results: Significant (p<0.001) upregulation was observed in E2F5 expression among tumor tissues, relative to the control group. These samples were hypo-methylated at the E2F5 promoter region in the tumor tissues, compared to the control. Change in the methylation status (Δmeth) was significantly lower (p=0.022) in the tumor samples, indicating possible involvement in tumorigenesis. Patients at the postmenopausal stage showed higher methylation (75%) than those at the premenopausal stage (23.1%). Interestingly, methylation levels gradually increased from the early to the advanced stages of the disease (p<0.001), which suggests a putative role of E2F5 methylation in disease progression that can significantly modulate tumor biology at more advanced stage and at postmenopausal age (Pearson’s r=0.99 and 0.86, respectively). Among tissues with different histological status, methylation frequency was higher in invasive lobular carcinoma (80.0%), followed by invasive ductal carcinoma (46.7%) and ductal carcinoma in situ (20.0%). Conclusion: Methylation is an important epigenetic factor that might be involved in the upregulation of E2F5 gene in tumor tissues, which can be used as a prognostic marker for breast cancer.

The Impact of Foreign Remittances and Financial Development on Poverty and Income Inequality in Pakistan: Evidence from ARDL - Bounds Testing Approach

Rizwana Kousar,Syed Imran Rais,Abdul Mansoor,Khalid Zaman,Syed Tahir Hussain Shah,Shakira Ejaz 한국유통과학회 2019 The Journal of Asian Finance, Economics and Busine Vol.6 No.1

The objective of the study is to examine the impact of financial development and foreign remittances on poverty and income inequality in the context of Pakistan. The study used ARDL-Bounds testing approach for robust inferences. The results show that in the short-run, remittances increases poverty and income inequality, which further translated into its long-run impact. The result confirmed the inverted Ushaped relationship between per capita income and income inequality, while the second order coefficient of per capita income substantially decline poverty incidence in a country. In the long-run, the results disappeared and it’s turned into U-shaped relationship between income inequality and country’s per capita income. Education largely decreases income inequality both in the short and long-run, however, it increases poverty in the long-run. Unemployment rate substantially damaged the pro-poor growth scenario, as high unemployment rate increases both the poverty rates and income inequality, which suffered poor more than non-poor in a country. Financial development has a positive impact on poverty reduction and income inequality in the short-run. The impact of income inequality on poverty incidence is positive both in the short- and long-run, which need pro-poor growth policies and rationale income distribution in a country.

GSTP1 Gene Ile105Val Polymorphism Causes an Elevated Risk for Bladder Carcinogenesis in Smokers

Pandith, Arshad Ahmad,Lateef, Adil,Shahnawaz, Sheikh,Hussain, Aashaq,Malla, Tahir Mohiuddin,Azad, Niyaz,Shehjar, Fahim,Salim, Mosin,Shah, Zafar Amin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Background: The glutathione S transferase (GST) family of enzymes plays a vital role in the phase II biotransformation of environmental carcinogens, pollutants, drugs and other xenobiotics. GSTs are polymorphic and polymorphisms in GST genes have been associated with cancer susceptibility and prognosis. GSTP1 is associated with risk of various cancers including bladder cancer. A case control study was conducted to determine the genotype distribution of GSTP1 A>G SNP, to elucidate the possible role of this SNP as a risk factor in urinary bladder cancer (UBC) development and to examine its correlation with clinico-pathologic variables inUBC cases. Materials and Methods: Using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) approach, we tested the genotype distribution of 180 bladder cancer patients in comparison with 210 cancer-free controls from the same geographical region with matched frequency in age and gender. Results: We did not observe significant genotype differences between the control and bladder cancer patients overall with an odds ratio (OR)=1.23 (p>0.05). The rare allele (AG+GG) was found to be present more in cases (28.3%) than in controls (24%), though the association was not significant (p<0.05). However, a significant risk of more than 2-fold was found for the variant allele (AG+GG) with smokers in cases as compared to controls (p>0.05). Conclusions: Thus, it is evident from our study that GSTP1 SNP is not implicated overall in bladder cancer, but that the rare, valine-related allele is connected with higher susceptibility to bladder cancer in smokers and also males.

Impact of the extent of resection of neuroendocrine tumor liver metastases on survival: A systematic review and meta-analysis

Rugved Kulkarni,Irfan Kabir,James Hodson,Syed Raza,Tahir Shah,Sanjay Pandanaboyana,Bobby V. M. Dasari 한국간담췌외과학회 2022 Annals of hepato-biliary-pancreatic surgery Vol.26 No.1

In patients with neuroendocrine tumors with liver metastases (NETLMs), complete resection of both the primary and liver metastases is a potentially curative option. When complete resection is not possible, debulking of the tumour burden has been proposed to prolong survival. The objective of this systematic review was to evaluate the effect of curative surgery (R0-R1) and debulking surgery (R2) on overall survival (OS) in NETLMs. For the subgroup of R2 resections, outcomes were compared by the degree of hepatic debulking (≥ 90% or ≥ 70%). A systematic review of the literature was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guidelines using PubMed, Medline, CINAHL, Cochrane, and Embase databases. Hazard ratios (HRs) were estimated for each study and pooled using a random-effects inverse-variance meta-analysis model. Of 538 articles retrieved, 11 studies (1,729 patients) reported comparisons between curative and debulking surgeries. After pooling these studies, OS was found to be significantly shorter in debulking resections, with an HR of 3.49 (95% confidence interval, 2.70–4.51; p < 0.001). Five studies (654 patients) compared outcomes between ≥ 90% and ≥ 70% hepatic debulking approaches. Whilst these studies reported a tendency for OS and progression-free survival to be shorter in those with a lower degree of debulking, they did not report sufficient data for this to be assessed in a formal meta-analysis. In patients with NETLM, OS following surgical resection is the best to achieve R0-R1 resection. There is also evidence for a progressive reduction in survival benefit with lesser debulking of tumour load.