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세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구
이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1
Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.
홍택근,이용림,임무현,정낙현 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2004 한국식품저장유통학회지 Vol.11 No.1
솔잎의 기능성에 관한 연구를 위하여 솔잎발효추출물(PFE)과 에탄올추출물(PE 80, PE 50)로 효소적 저해활성과 아질산염 소거작용에 대하여 연구하였다. Tyrosinase의 저해효과는 솔잎발효 추출물인 PFE가 솔잎 에탄올 추출물 PE 80과 PE 50에 비해 약 5∼38%정도 저해활성 효과가 높게 나타났다. XOase 저해효과는 PFE가 62.77%, PE 80이 64.90%, PE 50이 55.91%의 저해율을 나타내였으며 ACE저해효과는 PFE가 78.02%, PE 80이 69.82%, PE 50이 21.75%의 순으로 솔잎발효 추출물인 PFE가 가장 높은 저해효과를 나타내었다. 아질산염 소거작용은 솔잎추출물 모두 pH 3.0이하에서 높은 분해능력을 나타내였다. 유기산 함량 분석 결과, PFE, PE 80, PE 50의 세 시료 모두 항산화작용에 관여하는 ascorbi acid가 가장 많이 함유되어 있었으며, 시료별 ascorbic acid의 함량은 솔잎 발효추출물인 PFE가 가장 많이 함유되어 있는 것으로 나타났다. Effects on the physiological functionality, such as tyrosinase, xanthine oxidase, angiotensin converting enzyme and Nitrite scavenging ability were also observed by pine needle fermentation extract(PFE) and the difference in the consistency of pine needle ethanol extracts(PE 80, PE 50) was found. In the inhibition effect on tyrosinase, PFEP showed 5-38% higher than that of PE 80 and PE 50. In the inhibition on XOase, PFE, PE 80 and PE 50 showed 62.77%, 64.390%, 55.91% respectively. In the inhibition effect on ACE, PFE, PE 80 and PE 50 showed 78.02%, 69.82% and 21.75% respectively. Among these, PFE showed the highest ACE inhibition effect. In the inhibition effect on nitrite scavenging anility, the pine needle extracts showed a high effect in pH 3.0. As the result of the research using HPLC for the organic and, all the samples(PFE, PE 80, PE 50) showed higher contents of the ascorbic acid concerned with the effect of the antioxidative. PFE showed the highest contents of the ascorbic acid.
A Study on Effective Satellite Selection Method for Multi-Constellation GNSS
Taek Geun Lee,Yu Dam Lee,Hyung Keun Lee 사단법인 항법시스템학회 2023 Journal of Positioning, Navigation, and Timing Vol.12 No.1
In this paper, we propose an efficient satellite selection method for multi-constellation GNSS. The number of visible satellites has increased dramatically recently due to multi-constellation GNSS. By the increased availability, the overall GNSS performance can be improved. Whereas, due to the increase of the number of visible satellites, the computational burden in implementing advanced processing such as integer ambiguity resolution and fault detection can be increased considerably. As widely known, the optimal satellite selection method requires very large computational burden and its realtime implementation is practically impossible. To reduce computational burden, several sub-optimal but efficient satellite selection methods have been proposed recently. However, these methods are prone to the local optimum problem and do not fully utilize the information redundancy between different constellation systems. To solve this problem, the proposed method utilizes the inter-system biases and geometric assignments. As a result, the proposed method can be implemented in real-time, avoids the local optimum problem, and does not exclude any single-satellite constellation. The performance of the proposed method is compared with the optimal method and two popular sub-optimal methods by a simulation and an experiment.
P128 : Treatment of recalcitrant warts with quadrivalent HPV vaccine
( Taek Geun Lee ),( Hyun Hwang Bo ),( Tae Gwang Kwon ),( Se Won Jung ),( Young Seok Lee ),( Sook Kyung Lee ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2
Background: Warts are benign epidermal proliferations cause by HPV. Although two-thirds of warts in children show spontaneous regression in two years, recalcitrant warts can be very difficult to treat with traditional modalities. Objectives: This is a study to investigate the effect of quadrivalent HPV vaccine injection for patients with recalcitrant wart. Methods: From August 2012 to March 2013, thirteen patients were enrolled in the study after informed consent. HPV vaccine was administered in 3 doses at 0, 2, and 6 months. Each visit, the grade of improvement and satisfaction in treatment were measured by patients and investigator. Results: Overall, the response rate of recalcitrant warts was 84.6%. Six patients (46.2%) had complete remission, five patients(38.5%) had partial remission and 2 patients(15.4%) had none remission. There are no statistically significant differences in sex(p=0.612), age(p=0.332), number of lesion(p=0.575), and history of previous therapy(p=0.222) and combination therapy(p=0.777) among the complete, partial and none remission groups. Adverse effects were observed in 1 patients, and were syncope. Conclusion: Quadrivalent HPV vaccine treatment is an effective alternative treatment for recalcitrant wart.
Agmatine Reduces Hydrogen Peroxide in Mesangial Cells under High Glucose Conditions
( Geun Taek Lee ),( Hun Joo Ha ),( Hyun Chul Lee ),( Young Dong Cho ) 생화학분자생물학회 2003 BMB Reports Vol.36 No.3
Agmatine, an amine and organic cataion, reduced H₂O₂ that was generated by hyperglycemia, and transcription factors such as NF-кB and AP-1 activity in the mesangial cells that were exposed to high glucose. However, spermine which shares a strong nucleophillic structure with agmatine decreased the H₂O₂levels and AP-1, but not the NF-кB activity. Possible roles for agmatine and spermine in decreasing fibronectin are discussed, and the signaling pathway for agmatine-reduced fibronectin accumulation is presented.
Agmatine Reduces Hydrogen Peroxide in Mesangial Cells under High Glucose Conditions
Lee, Geun-Taek,Ha, Hun-Joo,Lee, Hyun-Chul,Cho, Young-Dong Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.3
Agmatine, an amine and organic cation, reduced $H_2O_2$ that was generated by hyperglycemia, and transcription factors such as NF-${\kappa}B$ and AP-1 activity in the mesangial cells that were exposed to high glucose. However, spermine which shares a strong nucleophilic structure with agmatine decreased the $H_2O_2$ levels and AP-1, but not the NF-${\kappa}B$ activity. Possible roles for agmatine and spermine in decreasing fibronectin are discussed, and the signaling pathway for agmatine-reduced fibronectin accumulation is presented.