Identification of Redox Sensitive Proteins in Prdx II Deficient Red Blood Cells

Tae‐Hoon Lee,Hee‐Young Yang,Hoon‐In Choi,Gia‐Buu Tran,Ung Yang 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

Red blood cells (RBCs) have been studied as models for infectious diseases, various symptoms of anemia, hemolysis, and erythrocyte aging. Although do not directly affect RBCs, other diseases may cause RBC physiological alterations that could be advanced for diagnostic aim or to convince better understanding of a certain pathological pattern. In this study, comparative RBC proteomics between healthy and abnormal conditions involve to promote aging related‐biomarker discovery. Peroxiredoxin II (Prdx II; a typical 2‐Cys Prdx) has been originally isolated from erythrocytes, and its structure and peroxidase activity have been adequately studied. Mice absent to Prdx II proteins had heinz bodies in their peripheral blood, and morphologically aged cells were detected in the dense RBC fractions, which contained markedly higher levels of reactive oxygen species (ROS). In addition, a labeling experiment with the thiol‐modifying reagent biotinylated iodoacetamide (BIAM) in Prdx II‒/‒ mice revealed that a variety of RBC proteins were highly oxidized. To identify oxidation‐sensitive proteins in Prdx II‒/‒ mice, we performed RBC comparative proteome analysis by nano‐UPLC‐MSE shotgun proteomics with relative protein quantitative analysis. We found oxidation‐sensitive 18 membrane and 41 cytosol proteins from 32 and 85 peptides containing cysteine oxidation, and analyzed comparative expression pattern in healthy RBCs of Prdx II+/+ mice (W1), healthy RBCs of Prdx II‒/‒ mice (K1), and abnormal RBCs of Prdx II‒/‒ mice (K2). These proteins belonged to cellular functions related with RBC lifespan maintain, such as cytoskeleton, stress‐induced proteins, amino acid/nucleic acid metabolic enzymes, signal transduction, and molecular transporters. Furthermore, protein networks among identified oxidation sensitive proteins were analyzed to associate with aging consequence. Consequently, we expected that RBC proteome may provide clues to understand redox‐imbalanced diseases.

Poster Session : PS 0831 ; Upper GI Tract : Clinical Factors to Predict Angiographically Detectable Non-Variceal Upper Gastrointestinal Bleeding in Patients Refractory to Endoscopic Treatment

( Tae Hwan Ha ),( Tae Hoon Oh ),( Sung In Yu ),( Min Kim ),( Jong Wook Kim ),( Won Ki Bae ),( Jae Hyung Kim ),( Seung Suk Baek ),( Mi Jin Ryu ),( Ye Na Choi ),( Ji Young Park ),( Eileen L Yoon ),( Tae 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Non-variceal upper GI bleeding (NVUGIB) is a common medical problem that has signifi cant association with morbidity and mortality. Angiographic detection and subsequent transarterial embolization (TAE) is a primary treatment option when medical and endoscopic treatments fail. We investigated clinical factors that could affect the success of the angiographic detection and prognosis after TAE in patients with NVUGIB refractory to endoscopic therapy. Methods: A retrospective analysis of the clinical data was done in patients with failed endoscopic treatment who underwent angiography for the treatment of acute NVUGIB between May 2002 and May 2013. Patients were divided into detection or non-detection groups according to the presence of bleeding stigmata in angiographic fi nding. Rebleeding defi ned as subsequent bleeding event within 7 days and mortality within 30days were analyzed as outcome parameters after TAE following detection in angiography. Results: A total 45 patients 37 (male, mean age, 65.9±14.9 years) were analyzed and classifi ed as a detection group (n=25, 55.5%) and non-detection group (n=20, 44.6%). Peptic ulcers were the most common cause of refractory NVUGIB. Larger transfusion amount (5.7±3.9 unit vs. 3.5±2.8 unit; P=0.03), prolonged aPTT level (34.2±17.3 sec vs. 21.8±13.8 sec; P=0.01) and short time interval between last endoscopy and angiography (17.5±25.9 hours vs. 34.3±59.5 hours; P=0.04) were found to be signifi cant factors for predicting angiographic detection. TAE was performed in all patients detected in angiography. Rebleeding (44%) was significantly associated with higher Rockall score (8.3±1.5 vs. 6.6±2.4; P=0.046) and mortality (12%) was signifi cantly associated with higher Rockall score (9.3±0.6 vs. 7.1±2.2; P=0.002) and higher level of BUN (55.3±47.4 vs. 27.6±17.4; P=0.01). Conclusions: Clinical characteristics associated with angiographic detection in patients with NVUGIB refractory to endoscopic therapy were severe bleeding, bleeding tendency and early angiographic intervention. The Rockall score is useful parameter for predicting rebleeding and mortality after TAE.

Proteomic Analysis of Thiol Specific‐Protein Oxidation

Tae‐Hoon Lee,Hee‐Young Yang,Hoon‐In Choi,Gia‐Buu Tran,Ung Yang 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

Peroxiredoxin II (Prdx II; a typical 2‐Cys Prdx) has been originally isolated from erythrocytes, and its structure and peroxidase activity have been adequately studied. Mice absent to Prdx II proteins had heinz bodies in their peripheral blood, and morphologically abnormal cells were detected in the dense red blood cell (RBC) fractions, which contained markedly higher levels of reactive oxygen species (ROS). In this study, a labeling experiment with the thiol‐modifying reagent biotinylated iodoacetamide (BIAM) in Prdx I‒/‒ mice revealed that a variety of RBC proteins were highly oxidized. To identify oxidation‐sensitive proteins in Prdx II‒/‒ mice, we performed RBC comparative proteome analysis in membrane and cytosolic fractions by nano‐UPLC‐MSE shotgun proteomics. We found oxidation‐sensitive 54 proteins from 61 peptides containing cysteine oxidation, and analyzed comparative expression pattern in healthy RBCs of Prdx II+/+ mice, healthy RBCs of Prdx II‒/‒ mice, and abnormal RBCs of Prdx II‒/‒ mice. These proteins belonged to cellular functions related with RBC lifespan maintain, such as cytoskeleton, stress‐induced proteins, metabolic enzymes, signal transduction, and transporters. Furthermore, protein networks among identified oxidation sensitive proteins were analyzed to associate with various diseases. Consequently, we expected that RBC proteome may provide clues to understand redox‐imbalanced diseases.

Targeting Multiple Myeloma Cell Death with Polyphenol EGCG

Tae‐Hoon Lee,Hee‐Young Yang,Hoon‐In Choi,Ung Yang,Kyoung‐Jin Chung,Lina Ren 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

(‐)‐Epigallocatechin 3‐gallate (EGCG) is a potent antioxidant polyphenol in green tea that acts as an anticancer agent via both direct and indirect pathways. Although the relationship between EGCG’s anticancer effects and its antioxidant activity is not fully understood, it is known that EGCG stimulates production of reactive oxygen species (ROS), which induce oxidative stress leading to cell death. In IM9 multiple myeloma cells, EGCG acted in a dose‐ and time‐dependent manner to induce apoptotic cell death. Among the antioxidant enzymes expressed in IM9 cells, levels of peroxiredoxin (Prdx) V were selectively and significantly reduced by EGCG. Moreover, the ROS scavenger NAC completely inhibited EGCG‐induced apoptosis and PrdxV reduction, while overexpression of PrdxV, but not a PrdxVC48S mutant, protected IM9 cells from EGCG‐induced apoptosis. EGCG‐induced reductions in cell viability and PrdxV levels were also observed in primary CD138+ multiple myeloma cells from patients. These results suggest that PrdxV is a key target via which EGCG mediates its anticancer effects.

Peroxiredoxin V Regulates Aco2, Acadm, and Acox1 Activity in Hypoxic Kidney

Tae‐Hoon Lee,Hee‐Young Yang,Joseph Kwon,Hoon‐In Choi,Lina Ren,Ung Yang,Byung‐Ju Park,Zae Young Ryoo 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

Peroxiredoxin V, an atypical thioredoxin peroxidase, is widely expressed in mammalian tissues. In addition, Prdx V is localized in mitochondria, peroxisome, cytosol, and nucleus. Prdx V has been reported to protect a wide range of cellular environments as antioxidant enzyme, and its dysfunctions may be implicated in several diseases, such as cancer, inflammation, and neurodegenerative disease. Identification and relative quantification of proteins affected by Prdx V may help identify novel signaling mechanisms that are important for oxidative stress response. However, the role of Prdx V in the modulation of hypoxia‐related cellular response is not studied yet. In order to examine the function of endogenous Prdx V in hypoxic condition in vivo, we generated a transgenic mouse model with Prdx V siRNA expression controlled by U6 promoter. Of many tissues, the knockdown of Prdx V expression was displayed in kidney, lung, and liver, but not spleen and skin. We conducted on the basis of nano‐UPLC‐MSE proteomic study to identify the Prdx V‐affected protein networks in hypoxic kidneys. In this study, we identified protein networks associated with oxidative stress, fatty acid metabolism, and mitochondrial dysfunction. Our results indicated that Prdx V affected to regulation of kidney homeostasis under hypoxia stress.

정준상관 행렬도와 군집분석을 응용한 KLPGA 선수의 기술과 경기성적요인에 대한 연관성 분석

최태훈,최용석,Choi, Tae-Hoon,Choi, Yong-Seok 한국통계학회 2008 응용통계연구 Vol.21 No.3

정준상관 행렬도(canonical correlation biplot)는 정준상관분석에서 두 변수 집단에 의해서 측정된 다변량 자료에서 변수 집단 간의 관계와 개체들의 관계를 탐색하기 위한 2차원 그림이다. 이는 일반적으로 최용석 (2006, 1장)의 한 변수 집단에 의한 행렬자료에 대한 일반적인 행렬도를 두 변수 집단에 의한 행렬자료로 확장한 것으로 볼 수 있다. 최근에 Choi와 Kim (2008)은 개체들이 많은 대용량 자료에서 행렬도의 해석상 힘든 문제점을 지적하고 이를 극복하는 데 군집분석을 활용하는 방법을 제시하고 있다. 일반적인 행렬도에서 발생하는 대용량 자료에 대한문제는 정준상관 행렬도에서도 동일하게 발생하곤 한다. 본 연구에서는 2006년도 KLPGA 선수 중 상금 순위 상위 50명을 대상으로 정준상관 행렬도를 통해 기술요인변수군(평균 퍼팅수. 그린 적중율, 파 세이브율, 파 브레이크율)과 경기성적요인변수군(상금, 평균 타수)간의 관련성을 살펴보고 군집분석을 활용하여 각 선수들의 군집을 시도하려한다. Canonical correlation biplot is 2-dimensional plot for investigating the relationship between two sets of variables and the relationship between observations and variables in canonical correlation analysis graphically. In general, biplot is useful for giving a graphical description of the data. However, this general biplot and also canonical correlation biplot do not give some concise interpretations between variables and observations when the number of observations are large. Recently, for overcoming this problem, Choi and Kim (2008) suggested a method to interpret the biplot analysis by applying the K-means clustering analysis. Therefore, in this study, we will apply their method for investigating the relationship between skill and competition score factors of KLPGA players using canonical correlation biplot and cluster analysis.

하악의 위치 변화가 기도저항에 미치는 영향

최재갑,정태훈 경북대학교 의학연구소 1999 경북대학교병원의학연구소논문집 Vol.3 No.1

This study evaluated whether substantial airflow resistence changes occurred by changing jaw position in normal and snoring subjects. A case-control design was utilized to assess group differences. Subjects included 11 snoring patients and 10 non-snoring subjects. Airway resistance was assessed using a whole body plethysmograph. Subjects in this study had their mouth opening standardized to a position of 7 mm of vertical separation and the resistance was measured under the following conditions: normal jaw position and 2/3 maximum protrusive jaw position. The results were as follows: 1. The airway resistance was higher in snoring group than in non-snoring group. 2. Both groups had a significant decrease in their airflow resistance was jaw position. In conclusion, these data document that airflow resistance can be significantly influenced by jaw positioning. Moving the jaw in a protrusive position produced reduction of resistance.

Prominent Malar의 교정

최시호,설정현,정영식,이대훈 大韓成形外科學會 1987 Archives of Plastic Surgery Vol.14 No.1

The well defined prominent high cheek bones is classically a sign of beauty in western culture, since the ideal facial configuration is oval and persons with high cheek bones seem to retain their youghful appearance longer than average. But in our cultural background, the persons with prominent malar regions may be unlucky or unfortunate during her or his life andso want to reduce prominent malar regions. We performed reduction malarplasty with various approach methods in 6 patients who complained prominent malar regions with or without prominent zygomatic arch area, and after the operation the patients were satisfied with the results. This article present materials, preoperative check, operative method, result and discussion for the following malarplasty.

Acryl系 共重合體의 合成과 金屬添加物에 따른 光分解特性 硏究

최승재,김태훈 단국대학교 1998 論文集 Vol.33 No.-

The study of many kinds of degradable polymer is actively proceeding to solve the problems of the environmental pollution which are due to sudden increasement of the plastic waste matters. Nowadays, the MMA(Methyl methacrylate) and the BA(n-Butyl acrylate) are being in use variously, making use of them synthesized copolymer with a method of the free radical initiated solvent polymerization. used Ethyl acetate as a solvent, Benzoyl peroxide as an initiator. The structure of the synthetic copolymers were confirmed making use of the ^1H-NMR and the FT-IR. And 0.001mol, 0.005mol and 0.01mol of photodegradable activators, such as Cu_2O and FeCl_3, to the synthetic copolymer were added. The film of the synthetic copolymer were make with a method of the solvent casting and then the properties of photodegradation effects of metal additives were studied. For photodegradation of the copolymers, irradiated ultraviolet light of wave length of longer than 250nm from the 450W medium pressure mercury lamp was irradiated. By 24hours intervals, the sample was gathered to measure the change of the characteristic peaks making use of the ^1H-NMR and the FT-IR. After irradiation: acrylic moidies of the copolymer were based on cain scission. The degree of photodegradation was founded on the irradiated time and the metal additives. For the photodegradation, the copolymer which have the metal additives was more efficient than a simple copolymer. Among the metal additives, FeCl_3 was more effective than Cu_2O. In Cu_2O’s case, the photodegradation tended to be decreased according th toe increasing concentration.

양극산화와 절연파괴로 형성한 인산칼슘 함유 티타늄 산화피막의 특성

최원호,이용승,김동환,권용훈,배태성 大韓齒科器材學會 2003 대한치과재료학회지 Vol.30 No.1

This study was performed to investigate the surface properties of electrochemically oxidized pure titanium by anodic spark deposition technique. Titanium plates of 10×10×1 ㎜ were polished sequentially from # 300 to # 1,000 emery paper. They were ultrasonically washed in ethyl alcohol and acetone for 5 min and dried in a desiccator. Titanium plates and titanium screw implants were anodized in an electrolytic solution that was dissolved calcium and phosphate in water. The electrolytic voltage was set in the range of 190-340 V and the current density was 30 ㎃/㎠. The surface oxide film was characterized by X-ray deffractometer(XRD), scanning electron microscope(SEM) and X-ray photoemission spectroscopy(XPS). The main crystal structure of the titanium oxide was anatase type as analyzed with thin-film X-ray diffractometery. The diameter of micropores increased with increasing the spark forming voltage. The porous coatings showed the growth of cell structure and more compact texture with increasing the spark forming voltage. Microcracks were examined in the coating formed at voltage of 340 V. Ca/P ratio in coatings was increased with increasing the voltage.